RNA_Brochure_2012JPCS55_0913.indd

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1 RNA produced by RNA Ambion

2 RNA Ambion Confidence comes naturally Ambion 20RNA RNA RNA 2

3 4 6 Total RNA 12 mrna mirna 20 RNase 22 RNA 3

4 RNA Ambion RNA RNA RT-PCR qpcr RNA RNA PureLink RNA Mini Kit PureLink Pro 96 Total RNA Purification Kit MagMAX -96 Total RNA Isolation Kit 24 High Throughput Web : Total RNA purification P6 mirna solutions P18 Cells-to-CT kits P14 NEW Single Cell-to-CT kits P17 RNA purification products selection guide Ambion Technical Resources F MagMAX FFPE Total Nucleic Acid Isolation Kit RecoverAll Total Nucleic Acid Isolation Kit for FFPE 1 50 Low-Middle Throughput 17 High Throughput 16 Low Throughput 4

5 TRIzol Plus RNA Purification System 1 50 Low-Middle Throughput PureLink Pro 96 Total RNA Purification Kit MagMAX -96 for Microarrays Total RNA Isolation Kit 24 High Throughput TRIzol Plus RNA Purification System MagMAX Total Nucleic Acid Isolation Kit Cells-to-CT kits cells Single Cell-to-CT Kit Single cell PureLink RNA Mini Kit 1 50 Low Throughput PureLink Pro 96 Total RNA Purification Kit MagMAX -96 Total RNA Isolation Kit 24 High Throughput 24 or PCR PCR PCR FFPE 500 µl LCM 1 50 Low-Middle Throughput 24 High Throughput RNA Single Cell-to-CT Kit PureLink RNA Micro Kit 3 ml TRIzol LS Reagent MagMAX -96 Blood RNA Isolation Kit mirvana mirna Isolation Kit or RNA PureLink Total RNA Blood Kit Tempus tubes Stabilized Blood-to-CT kits LeukoLOCK Kit MagMAX for Stabilized Blood Tubes kits Tempus kits 5

6 Total RNA PureLink RNA Mini Kit Total RNA PureLink RNA Mini Kit Total RNA 1PureLink RNA Mini Kit 1 RNA RNA RNase 20RNA 1,000 µg RNA RNA PureLink 20 RNARNA DNA RT-PCR qpcr cdna Mini KitMidi Kit Total RNA23 PureLink RNA Mini Kit 1 PureLink RNA Mini Kit 1 4 RNA SPIN mg 250 mg 0.2 ml 5 x , RNA RNA (µg) PureLink RNA Mini Kit Qiagen Q RNA RNeasy Midi Kit RNA Midi Kit Q RNA Mini Kit SPIN 3 RNA Tissue (mg) 2 PureLink RNA Mini Kit: 200 mg PureLink RNA Mini Kit Q Kit Mini Midi25 mg 100 mg 200 mg RNA Total RNA PureLink RNA Mini Kit Q Midi Kit PureLink RNA Mini Kit 6

PureLink RNA Micro Kit RNA PureLink RNA Micro KitLCM FACS RNA TRIzol Reagent 10 mg 15100 µg RNA Total RNA 85 Total RNA Yield (ug) 20 18 16 14 12 10 8 6 4 2 0 Liver Spleen Kidney Brain Heart Testes

7 PureLink RNA Micro Kit RNA PureLink RNA Micro KitLCM FACS RNA TRIzol Reagent 10 mg µg RNA Total RNA 85 Total RNA Yield (ug) Liver Spleen Kidney Brain Heart Testes Rat Tissue Sample 1 Sample 2 Sample 3 3 PureLink RNA Micro Kit 5mg RNA RNA 10 l RNA 260nm 2 PureLink RNA Micro Kit Cells HeLa µg HEK µg Rat liver 5 mg 17 µg Rat brain 5 mg 2 µg Rat spleen 5 mg 13.1 µg Rat heart 5 mg 1.8 µg Rat testes 5 mg 7.1 g 3 PureLink PureLink RNA Mini Kit Q Mini Kit Q Midi Kit PureLink RNA Micro Kit Q Micro Kit 200 mg 5 x mg mg 5 x x mg 5 x mg 5 x ,000 µg 100 µg 1,000 µg 100 µg 45 µg µl µl µl µl µl Ordering information PureLink RNA Mini Kit 10 preps preps A 250 preps PureLink RNA Micro Kit 50 preps

Total RNA TRIzol Reagents TRIzol Reagents DNATotal RNA 4 Chomczynski Sacchi RNA TRIzol Reagents RNA RNA TRIzol ReagentsRNA 50 100 mg 5 x10 6 1 g >10 7 TRIzol Reagent 1 TRIzol Reagent Total RNADNA 4

8 Total RNA TRIzol Reagents TRIzol Reagents DNATotal RNA 4 Chomczynski Sacchi RNA TRIzol Reagents RNA RNA TRIzol ReagentsRNA mg 5 x g >10 7 TRIzol Reagent 1 TRIzol Reagent Total RNADNA 4 TRIzol Reagents TRIzol 100 mg ml TRIzol LS 5 x ml TRIzol Max Bacterial RNA Isolation Kit 10 8 Max Bacterial Enhancement Reagent 0.2 ml + TRIzol Reagent 1 ml RNA RNase RNase RNA RNA RNase Ordering information TRIzol Reagent 100 ml ml TRIzol LS Reagent 100 ml ml TRIzol Max Bacterial RNA Isolation kit TRIzol Reagent 100mL + Enhancement Reagent 20mL TRIzol Reagent 200mL + Enhancement Reagent 40mL

9 TRIzol Plus RNA Purification System RNA TRIzol Plus RNA Purification SystemTRIzol Reagent PureLink RNA Mini Kit 4 TRIzol Plus RNA Purification System 1 RNA 55 Total RNAqPCR 1,000 µg Total RNA RNA Total RNAg 1, TRIzol Plus RNA Purification System Q Mini Kit ,000 Starting material mg 5 TRIzol Plus RNA Purification System TRIzol Plus RNA Purification System Q Total RNA TRIzol Plus System 1,000 µg RNA Q 1/10 4 TRIzol Reagent RNA RNA TRIzol RNA SPIN SPIN RNA PureLink RNA RNA RNase RNA RNase RNA 5 TRIzol Plus RNA Purification System TRIzol Plus RNA Purification System Q Reagent+ Mini Kit TRIzol Reagent 100 ml + 50 Q Reagent 200 ml mg mg10 7 1,000 µg 100 µg µl µl Ordering information TRIzol Plus RNA Purification System 50 preps

Total RNA MagMAX purification systems RNA MagMAX TM MagMAX TM 96 6 7MagMAX TM 10 5 RNA RT- PCR100 RNA RNA 1 C t 40 35 30 Slope = -3.7 ±0.1 R 2 = 0.996 20 50 µlrna ΔRn 25 20 0.

10 Total RNA MagMAX purification systems RNA MagMAX TM MagMAX TM MagMAX TM 10 5 RNA RT- PCR100 RNA RNA 1 C t Slope = -3.7 ±0.1 R 2 = µlrna ΔRn Cell # Cycle 2. RNA 7 Total RNA MagMAX TM -96 Total RNA Isolation Kit x 10 6 K562 RNA 8 n=8 4 qrt-pcr 10 µl RNA Polymerase II mrna 8 n=8 Ct CV RNA 6 The MagMAX Isolation Kit 10

11 6 MagMAX TM Kits: MagMAX -96 Total RNA Isolation Kit MagMAX -96 for Microarrays Total RNA Isolation Kit 2 x x mg 10 mg Plant RNA Isolation Aid 100 mg gdnaturbo DNase TM mirna MagMAX -96 Viral RNA Isolation Kit MagMAX Total Nucleic Acid Isolation Kit MagMAX -96 Blood RNA Isolation Kit RNA DNA RNA DNA µl 175 µl 300 mg 175 µl 300 mg 50 µl DNA 50 µl gdna Ordering information MagMAX -96 Total RNA Isolation Kit 96 rxns AM1830 MagMAX -96 for Microarrays Total RNA Isolation Kit 96 rxns AM1839 MagMAX -96 Viral RNA Isolation Kit 50 rxns AM rxns AM x 96 rxns AMB18365 MagMAX Total Nucleic Acid Isolation Kit 100 rxns AM1840 MagMAX -96 Blood RNA Isolation Kit 96 rxns AM

12 mrna Total RNA Total RNAmRNA mrna Total RNA mrna 2 mrna Total RNA mrna dt/dt / 7 mrna dt/ Poly A Purist TM KitMicroPoly A Purist TM Kit rrna mrna 1 2mRNA FastTrack 2.0 mrna Isolation Kit mrnatotal RNA mrna FastTrack 2.0 mrna Isolation Kit mrna Total RNA mrna dt / Poly A Purist TM MAG Kit Poly A Purist TM Kit mg Total RNA µg Total RNA 7 mrna Total RNA mrna Poly(A)Purist MicroPoly(A)Purist Poly (A) Purist MAG Dynabeads mrna Purification MICROBExpress Bacterial mrna mrna FastTrack 2.0 mrna Isolation Dynabeads mrna DIRECT FastTrack MAG mrna Catcher PLUS dt Poly A RNA 96 mrna Total RNA 95rRNA 12

13 Dynabeads mrna Purification Kit for mrna Purification from Total RNA Preps Total RNAmRNA 15 5 µlmrna cdna Dynabeads mrna DIRECT TM 15 5 µlmrna cdna FastTrack MAG Kit mrna Total RNA dt FastTrack MAG Micro mrna Isolation Kit 50 mg Total RNA mg FastTrack MAG Maxi mrna Isolation Kit 2,000 µg Total RNA 5 x mg mrna Catcher TM PLUS Kit 100 ng 100 µg Total RNA mg 40 µl 80 ng MICROBExpress TM Bacterial mrna Enrichment mrnamrna mrna Total RNA16S 23S rrna 95% mrna Ordering information Poly(A)Purist Kit 6rxns AM1916 MicroPoly(A)Purist Kit 20rxns AM1919 FastTrack 2.0 mrna Isolation Kit 6rxns K rxns K Poly(A)Purist MAG Kit 1 kit AM1922 Dynabeads mrna Purification Kit 2mL Dynabeads mrna DIRECT Kit 5mL mL Dynabeads mrna DIRECT Micro Kit 2mL FastTrack MAG 96 mrna Isolation Kit 2 96 preps K FastTrack MAG Maxi mrna Isolation Kit 6 preps K FastTrack MAG Micro mrna Isolation Kit 12 preps K mrna Catcher PLUS Kit 1 x 96 preps K x 96 preps K MICROBExpress Bacterial mrna Enrichment Kit 20rxns AM

14 Cells-to-CT kits RNA RT-PCR Cells-to-CT TM Kits RT-PCRRNA RNA Cells-to-CT TM Kits TaqMan SYBR GreenPCR 8 8 Cell-to-CT TM Kits TaqMan Gene Expression Cells-to-CT Kit TaqMan PreAmp Cells-to-CT Kit TaqMan Fast Cells-to-CT Kit Application KitPCR master mix TaqMan Gene Expression Master Mix TaqMan PreAmp Reagents TaqMan Gene Expression Master Mix TaqMan Fast Universal PCR Master Mix 7 60 PCR PCR PCR , , ,000 sirna Pre-PCR Pre-PCR Fast PCR PCR 40 Fast Ordering information TaqMan Gene Expression Cells-to-CT Kit 40rxns 100PCRs rxns 500PCRs 400rxns 2,000PCRs AM1728 AM1729 TaqMan PreAmp Cells-to-CT Kit 40rxns 500PCRs TaqMan Fast Cells-to-CT Kit 100rxns 500PCRs TaqMan MicroRNA Cells-to-CT Kit 100rxns 500PCRs Power SYBR Green Cells-to-CT Kit 40rxns 100PCRs rxns 500PCRs rxns 2,000PCRs

15 7 10 min total 5x 5' 5' 3' 3' 3' mrna 5' RT primer 3' 5' cdna RT 3. PCR 1PBS RT 2 RT65 PCR cdna 2 PCR PCR 8 Cells-to-CT TM Kit TaqMan MicroRNA Cells-to-CT Kit Power SYBR Green Cells-to-CT Kit Fast SYBR Green Cells-to-CT Kit Stabilized Blood-to-CT Single Cell-to-CT mirna TaqMan Universal PCR Master Mix Power SYBR Green PCR Master Mix Fast SYBR Green Master Mix TaqMan Gene Expression Master Mix PCR PCR PCR , , , µl 1 10 TaqMan Micro RNA Assays Fast PCR PCR 40 Fast Total RNA mirna PAXgene Tempus TM Blood RNA Tubes RT-PCR Ordering information Fast SYBR Green Cells-to-CT Kit 100rxns 500PCRs rxns 2,000PCRs Stabilized Blood-to-CT Nucleic Acid Preparation Kit for qpcr (PAXgene or Tempus Blood RNA Tubes) Stabilized Blood-to-CT Nucleic Acid Preparation Kit for qpcr (PAXgene Blood RNA Tubes) Stabilized Blood-to-CT Nucleic Acid Preparation Kit for qpcr (Tempus Blood RNA Tubes) 50rxns rxns rxns Ambion Single Cell-to-CT Kit 50rxns rxns Cells-to-CT family 15

Cells-to-CT kits RNA Cells-to-CT Cells-to-CT 8 RT-PCR RNA 9 RNA 10 Panel A. 18S rrna Cells-to-CT Lysate Purified RNA Cells-to-CT Lysate Purified RNA Panel B.

ACTB 9 Cells-to-CT Kit RT-PCR HeLa RNA Cell-to-CT Kit 128 TaqMan Gene Expression Assays Cell-to-CT Kit CT Cell-to-CT Kit RT- 10 Cells-to-CT Kit RNA 10 100,000 HeLa RNA Taq- Man Gene Expression Master

16 Cells-to-CT kits RNA Cells-to-CT Cells-to-CT 8 RT-PCR RNA 9 RNA 10 Panel A. 18S rrna Cells-to-CT Lysate Purified RNA Cells-to-CT Lysate Purified RNA Panel B. GAPDH Cells-to-CT Lysate Purified RNA Panel C. ACTB 9 Cells-to-CT Kit RT-PCR HeLa RNA Cell-to-CT Kit 128 TaqMan Gene Expression Assays Cell-to-CT Kit CT Cell-to-CT Kit RT- 10 Cells-to-CT Kit RNA ,000 HeLa RNA Taq- Man Gene Expression Master Mix TaqMan Human Endogenous Control Arrays Panel A~C Endogenous Control Arrays 16 3 Ambion Cells-to-CT TM PCR YouTubeAmbion Cells RNA R 2 = 1.00 TaqMan Gene Expression Cells-to-CT TM Kit R 2 = 0.99 A R 2 = 1.00 Q R 2 = 0.99 C t TaqMan Gene Expression Cells-to-CT TM Kit Hela ,000 RNA TaqMan Gene Expression Cells-to-CT TM Kit Q A RNA PCR PPIA TaqMan Gene Expression Assay 3 Cells-to-CT TM Kit Kit RNA RNA E2 10 E3 10 E4 10 E5 16

Single Cell-to-CT Kit RT-PCR 5 13 14 Ambion Single Cell-to-CT Kit1-10 12 1. 2. 3. RT 4. 5. PCR 1. 10 Single Cell Solution 1 10 2. 5 1. 1 Stop Solution 2. 2 1. 4-5 RT Mix 2. 25 10 42 60 85 5 1.

17 Single Cell-to-CT Kit RT-PCR Ambion Single Cell-to-CT Kit RT PCR Single Cell Solution Stop Solution RT Mix PreAmp Mix PCR 1. Preamplified 1 20 PCR 12 Ambion Single Cell-to-CT Kit Single Cell-to-CT Kit 13 Single Cell-to-CT Kit CT Single Cell Lysis Buffer PBS / 5 3. PBS RT cdna 14 Pre-Amplification B2M GAPDH HPRT1 TGFB1 5 CT 17

18 mirna mirna mirna Small RNA RNA mirnanon-coding RNA22 mrna mirna Small RNA mirna mirna RT-PCR 9 RNA 200 nt Small RNA mirvana mirna Isolation Kit RNA Total RNA RNA 200 nt mirna sirna shrna snrna 9miRNA mirvana mirna Isolation Kit mirvana PARIS Kit RNA mirna Total RNA DNA ü ü ü mg mg mirna RNA 30 mrna mirnasirna RNA Small RNA Ordering information mirvana mirna Isolation Kit 40 rxns AM1560 mirvana PARIS Kit 40 rxns AM

19 TaqMan MicroRNA Cells-to-CT Kit RNA mirna , RT-PCRTaqMan MicroRNA Assays RecoverAll Total Nucleic Acid Isolation Kit for FFPE Tissues FFPE RNA RNA overnight ProteinaseK RT-PCR 50% MagMAX -96 for Microarrays Total RNA Isolation Kit TaqMan MicroRNA Cells-to-CT Kit RecoverAll Total Nucleic Acid Isolation Kit for FFPE Tissues ü ü ü ü Cells-to-CT TM Kit DNase 5 x mg , µm RNA RNA RT-PCR TaqMan MicroRNA Assays FFPE RNA RNAovernight Proteinasek RT-PCR 50% RT-PCR PCR Ordering information MagMAX -96 for Microarrays Total RNA Isolation Kit 96 rxns AM1839 TaqMan MicroRNA Cells-to-CT Kit 100 rxns (500 PCRs) rxns (2,000 PCRs) RecoverAll Total Nucleic Acid Isolation Kit for FFPE Tissues 40 rxns AM

RNase Ambion RNA Essentials Products RNase RNase RNase RNA 1 RNase 2 3RNase RNase DNase nuclease-free nuclease RI RNA3714 16 RNA RNaseZap Solution RNaseZap Wipes ElectroZap TM Solution RNase AWAY

20 RNase Ambion RNA Essentials Products RNase RNase RNase RNA 1 RNase 2 3RNase RNase DNase nuclease-free nuclease RI RNA RNA RNaseZap Solution RNaseZap Wipes ElectroZap TM Solution RNase AWAY Reagent RNase RNaseZap Solution RNase RNase RNaseZap Wipes l / Hind III Markers A B RNaseZap Ordering information Conical Tubes (15 ml) (racked) 500 tubes AM12500 Conical Tubes (50 ml) (racked) 200 tubes AM12501 Nuclease-Free Water (not DEPC-treated) 500 ml AM9930 (1 x 500 ml) DEPC-Treated Water (1 x 100 ml) 100 ml AM9915G RNase-free Tip Tube RNase-free Microfuge Tubes 1,000 x 0.5 ml AM12300 RNase-free Microfuge Tubes 500 x 1.5 ml AM12400 RNase-free Microfuge Tubes 500 x 2.0 ml AM12425 Non-stick RNase-free Microfuge Tubes 500 x 0.5 ml AM12350 Non-stick RNase-free Microfuge Tubes Non-stick RNase-free Microfuge Tubes Elution Tubes RNase-free Tips, 200 μl Size RNase-free Tips, 1000 μl Size Barrier (Filter) Tips, 1 ml Size Barrier (Filter) Tips, 200 μl Size Barrier (Filter) Tips, 100 μl Size Barrier (Filter) Tips, 20 μl Size Barrier (Filter) Tips, 10 μl Size -Pipetman Barrier (Filter) Tips, 10 μl Size -Eppendorf Thin-walled, Dome Cap, RNase-free PCR Tubes Thin-walled, Frosted Lid, RNase-free PCR Tubes Thin-walled, Frosted Lid, RNase-free PCR Tubes RNaseZap Solution 250 x 1.5 ml AM x 2.0 ml AM x 2.0 ml AM AM12650 (8 x 12 / ) 10 AM12660 (100 / ) 10 AM12665 (100 / ) 10 AM12655 (8 x 12 / ) 10 AM12648 (8 x 12 / ) 10 AM12645 (8 x 12 / ) 10 AM12640 (8 x 12 / ) 10 AM12635 (8 x 12 / ) 1,000 x 0.5 ml AM ,000 x 0.5 ml AM ,000 x 0.2 ml AM12225 RNaseZap Solution (1 bottle) 250 ml AM9780 RNaseZap Solution (6 bottles) 6 x 250 ml AM S rrna 18S rrna RNase RNaseZap Solution (1 box) 4 L AM9784 RNaseZap Wipe RNaseZap Wipes 100 AM9786 RNaseZap Wipes Refill 3 x 100 AM

RNase RNA RNA RNA RNA RNA RNAlater RNA : RNaseRNA 371251 41-20RNA RNAlater Solution : cdna RNA RNA RNA RNAlater -ICE RNA RNA RNA RNA Storange Solution RNA 0.

RNAlater Solution 500 ml AM7021 RNAlater Solution 50 x 1.

21 RNase RNA RNA RNA RNA RNA RNAlater RNA : RNaseRNA RNA RNAlater Solution : cdna RNA RNA RNA RNAlater -ICE RNA RNA RNA RNA Storange Solution RNA 0.1mµ EDTA TE Buffer RNAlater Solution RNAlater Solution XenopusDrosophila RNAlater Solution RNaseRNA 37 C 4 C RNA RNA p53 GAPDH p53 RNAlater Solution RNAlater Solution 100 ml AM7020 RNAlater Solution 500 ml AM7021 RNAlater Solution 50 x 1.5 ml AM7022 RNAlater Solution 20 x 5 ml AM7023 RNAlater Solution 250 ml AM RNAlater RNA GAPDH 5 RNAlater p53 GAPDH RNAlater RNA RNase RNAlater -ICE Solution RNAlater -ICE Solution 25 ml AM7030 RNAlater -ICE Solution ml RNA Strage Solution THE RNA Strange Solution 10 tubes AM7000 THE RNA Strange Solution 50 ml AM

01 10ng/ L 10 replicates of lambda DNA Qubit DNA HS Assay

22 RNA Qubit 2.0 Fluorometer Qubit 2.0 Fluorometer DNA RNA RT-PCR(qPCR) Qubit 2.0 FluorometerMolecular Probes Qubit DNA RNA 17 Qubit 2.0 Fluorometer ng/ L 10 replicates of lambda DNA Qubit DNA HS Assay kit Qubit 2.0 Fluorometer Spectrophotometer Qubit 17DNA RNA Qubit 10 Qubit 10 pg/µl DNA 12.5 µg/ml 16 The Qubit Fluorometric Quantitation System 22

2-3 2 Protein Assay 15 Qubit 2.0 18 Qubit Qubit 2.0Fluorometer 10 Qubit Qubit dsdna HS Assay 0.

23 2-3 2 Protein Assay 15 Qubit Qubit Qubit 2.0Fluorometer 10 Qubit Qubit dsdna HS Assay ng 10 pg 100 ng/µl Qubit dsdna BR Assay 2 1,000 ng 100 pg 1 µg/µl Qubit ssdna Assay ng 50 pg 200 ng/µl Qubit RNA Assay ng 250 pg 100 ng/µl Qubit RNA BR Assay 20 1,000 ng 1 ng 1 µg/µl Qubit Protein Assay* µg 12.5 µg 5 mg/ml Qubit Protein Assay 0.01% SDS2 µl Ordering information Qubit 2.0 Fluorometer Q32866 Qubit 2.0 Quantitation Starter Kit 1 Kit Q32871 Qubit 2.0 Quantitation Lab Starter Kit 1 Kit Q32872 Qubit dsdna BR Assay Kit 100 assays Q assays Q32853 Qubit dsdna HS Assay Kit 100 assays Q assays Q32854 Qubit ssdna Assay Kit 100 assays Q10212 Qubit RNA Assay Kit 100 assays Q assays Q32855 Qubit RNA BR Assay Kit 100 assays Q assays Q10211 Qubit Protein Assay Kit 100 assays Q assays Q33212 Qubit Assay Tubes 500 tubes Q32856 Qubit 23

24 Facebook! facebook.com/lifetechnologiesjapan TEL FAX TEL FAX FAX TEL FAX TEL FAX TEL FAX TEL FAX The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. TaqMan is a registered trademark of Roche Molecular Systems, Inc. TRIzol is a registered trademark of Molecular Research Center, Inc , Life Technologies Japan Ltd. All rights reserved. Printed in Japan. AMB126-A1209OB

RNA_Brochure_2012JPCS55_0913.indd

RNA_Brochure_2012JPCS55_0913.indd RNA produced by RNA Ambion RNA 2014 10 produced by RNA Ambion Total RNA P6 PureLink RNA Mini Kit 10 preps 12183020 9,700 PureLink RNA Mini Kit 50 preps 12183018A 30,000 PureLink RNA Mini Kit 250 preps