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1 Agilent 4200 TapeStation Agilent 4200 TapeStation
2 3 1 2
3 2 3 ScreenTape 3
4 Agilent 4200 TapeStation QC Agilent 4200 TapeStation Ready-to-use ScreenTape 8 PCR 96-well plate qrt-pcr DNA RNA DNA / RNA QC Agilent 4200 TapeStation TapeStation Vortexer ScreenTape 8 2TapeStation TestTape 96-well plate 96-well plate Foil Seal Loading tip 8 PCR tube strip & cap 4
5 Tape Rack well plate 8 96 Loading tip 1 5
6 Agilent 4200 TapeStation ScreenTape Agilent 4200 TapeStation ScreenTape DNA RNA Genomic DNA D5000 / High Sensitivity D5000 D1000 / High Sensitivity D1000 RNA / High Sensitivity RNA ,000 bp 100 5,000 bp 35 1,000 bp Total RNA CGH PCR QC QC PCR qrt-pcr RNA-Seq QC ScreenTape 1 ScreenTape 16 * * 2 Assay Lot 2 Assay Lot 6
7 DNA Genomic DNA D5000 High Sensitivity D5000 D1000 High Sensitivity D >60,000 bp 100 5,000 bp 100 5,000 bp 35 1,000 bp 35 1,000 bp *1 0.5 ng/µl 0.1 ng/µl 5 pg/µl 0.1 ng/µl 5 pg/µl 400 5,000 bp 15% 400 5,000 bp 15% *2 *2, ,000 bp 15% CV ,000 bp 15% ng/µl DIN ng/µl *4 * ng/µl 15% CV 15% CV 1 50 ng/µl 10% CV bp 15%, 300 1,000 bp 10% bp 15%, 300 1,000 bp 10% 5 % CV 10 % CV 5% CV 5% CV 10 % 15 % 10% *3 10% * ng/µl 10 1,000 pg/µl ng/µl 10 1,000 pg/µl 15% CV ng/µl 15% CV 1 50 ng/µl 10% CV 15% CV *4, 5 20% *4 20 % *5 25 % *5 20% *5 20% *5 15 < < mm KCl, 250 mm Tris-HCl, 125 mm NaCl, 50 mm Acetate, 25 mm MgCl2, 25 mm BSA, 25 mm Guanidine-HCl 15 < < mm KCl, 25 mm Tris-HCl, 12.5 mm NaCl, 5 mm Acetate, 2.5 mm MgCl2, 2.5 mm BSA, 2.5 mm Guanidine-HCl 15 < < mm KCl, 60 mm Phosphate Buffer, 60 mm Guanidine-HCl, 240 mm NaCl, 60 mm Acetate 16 < < 90 7 mm KCl, 20 mm Phosphate Buffer, 20 mm Guanidine-HCl, 80 mm NaCl, 20 mm Acetate 16 < < 120 /1 Tape *6 15 *6 15 *6 15 * µl 1 µl 2 µl 1 µl 2 µl *1 S/N 3 *2 *3 20% *4 Genomic DNA * TapeStation *6 1 Tape 1 Ladder RNA RNA High Sensitivity RNA *1 5 ng/µl 100 pg/µl RIN e ng/µl ,000 pg/µl 1,000 25,000 pg/µl * 10% CV 15% CV 20% 30% 200 mm Tris 20 mm EDTA 50 mm NaCl 16 < < mm Tris 1 mm EDTA 16 < < 180 /1 Tape µl 2 µl *1 Total RNA TE S/N 3 * TapeStation 7
8 Agilent 4200 TapeStation DNA Genomic DNA CGH DNA DNA TapeStation DNA DNA Integrity Number DIN DNA DIN D5000 / High Sensitivity D5000 D1000 / High Sensitivity D1000 QC PCR D Size selected human genomic DNA Long-range PCR
9 RNA RNA / High Sensitivity RNA qrt-pcr total RNA TapeStation RNA total RNA RIN e RNA Integrity Number equivalentrin e RNA RIN RIN RIN e 9.0 RIN e 7.1 RIN e 6.1 RIN e 4.6 RIN e 3.6 RIN e 2.9 RINe 2.6 RNA-Seq total RNA TapeStation 1 Agilent Strand-Specific RNA Total RNA Total RNA poly A RNA poly A RNA RNA 2 cdna cdna PCR Total RNA QC QC bp RNA ScreenTape D1000 ScreenTape 9
10 Agilent 4200 TapeStation Agilent 4200 TapeStation / 4200 TapeStation Controller Software Assay ScreenTape 2 Assay CSV Electropherogram Region Assay TapeStation Analysis Software Observation Molarity RIN e DIN 10
![Sample Table Peak Table Well Conc. [ng/µl] Sample Description Alert Observations A1 33.7 Size Selected Human gdna 40ng Size [bp] Calibrated Conc. Assigned Conc.](/docs-images/89/100825886/images/11-0.jpg "Peak Molarity % Integrated Area Peak Comment Observations [ng/µl] [ng/µl] [nmol/l] 15 7.05-723 - Lower Marker 1040 33.7-49.9 100.00 10000 3.60 3.60 0.")
![554 - Upper Marker Region Table From [bp] To [bp] Average Size [bp] Conc. [ng/µl] Region Molarity % of Total Region Comment Color [nmol/l] 350 3000 1070 37.4 59.7 96.](/docs-images/89/100825886/images/11-1.jpg "44 Size Selected Files used in the Profile Comparison File 3 PDF CSV XML D5000 ScreenTapeR Page 2 of 9 D5000 ScreenTapeR Page 1 of 9 Filename: Comparison_7_Lanes_f245.")
11 Sample Table Peak Table Well Conc. [ng/µl] Sample Description Alert Observations A Size Selected Human gdna 40ng Size [bp] Calibrated Conc. Assigned Conc. Peak Molarity % Integrated Area Peak Comment Observations [ng/µl] [ng/µl] [nmol/l] Lower Marker Upper Marker Region Table From [bp] To [bp] Average Size [bp] Conc. [ng/µl] Region Molarity % of Total Region Comment Color [nmol/l] Size Selected Files used in the Profile Comparison File 3 PDF CSV XML D5000 ScreenTapeR Page 2 of 9 D5000 ScreenTapeR Page 1 of 9 Filename: Comparison_7_Lanes_f245.cD5000 Gel Image A1: Size Selected Human gdna 40ng D5000 ScreenTapeR Page 9 of 9 Profile Comparison Default image (Contrast 100%), Image is Scaled to view larger Molecular Weight range Sample Info Well Conc. [ng/µl] Sample Description Alert Observations A Size Selected Human gdna 40ng B Size Selected Human gdna 20ng C Size Selected Human gdna 10ng D Size Selected Human gdna 5ng E Size Selected Human gdna 2.5ng F Size Selected Human gdna 1.25ng G Size Selected Human gdna 0.6ng Samples File Indicator Well ID Comment File 3 A1 Size Selected Human gdna 40ng Size Selected Human gdna 20ng Size Selected Human gdna 10ng D1 Size Selected Human gdna 5ng E1 Size Selected Human gdna 2.5ng F1 Size Selected Human gdna 1.25ng G1 Size Selected Human gdna 0.6ng File 3 File 3 File 3 File 3 B1 File 3 C1 File Comments Filename TapeStation Analysis Software A c Agilent Technologies, Inc Generated: 06-Sep-2015 TapeStation Analysis Software A c Agilent Technologies, Inc Generated: 06-Sep-2015 C:\Users\ytsumoto\Documents\02_4200\ ちらしなど \ カタログ \Comparison_7_Lanes_f245.cD5000 TapeStation Analysis Software A c Agilent Technologies, Inc Generated: 06-Sep-2015 TapeStation TestTape Agilent Lab Advisor Agilent Lab Advisor TapeStation TestTape Agilent Lab Advisor 11
12 G2991AA TapeStation vortex 25.5 kg 43.5 cm 50 cm 45.5 cm VAC, 50 / 60 Hz, 50 VA W / mail _japan@agilent.com Agilent Technologies, Inc Printed in Japan, Aug.15, JAJP
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