untitled

Size: px

Start display at page:

Download "untitled"

ひろみわかはら
5 years ago
Views:

1 ORYZA OIL & FAT CHEMICAL CO., LTD. WHITE JELLY FUNGUS EXTRACT -P () Ver. 1.3SJ

2 Ver. 1.3SJ ph 1

3 Ver. 1.3SJ SOD in vitro 3-2. in vivo 3-3. in vivo 3-4. in vivo in vivo 4-2. in vivo 4-3. in vivo in vitro 5-2. in vitro in vitro 7-2. in vivo in vivo in vivo

4 Ver. 1.3SJ

5 Ver. 1.3SJ α-1,3- D-D- D- 9143moler 17.6 % D-L- Man Man Man Man Man Man Man Man Man GluA GluA GluA Man Xyl Fuc Xyl Xyl Xyl

6 Ver. 1.3SJ 2-3. ph ph ph ph ph 1.8 ph () ph 1.8pH g No

7 Ver. 1.3SJ 2-4. SOD 4 Fenton SODNU/L mmol /L % % % % cont Cont. 4. SOD 0 0% 0.1% 0.5% 1.0 % Cont. 5. 6

8 Ver. 1.3SJ in vitro Kiho 1) Tremella sp.tptp G-6-P TP 2. TP Group Dose (mg/kg) Enzyme activity (mean ± S.E, n=5) 0 3 6(h) Glucokinase N: Control 10.8 ± ± ± 2.1 N: TP ± 2.6* 15.4 ± 2.0 D: Control 12.5 ± ± ± 1.6 D: TP ± 1.1* 19.5 ± 2.0* Hexokinase N: Control 19.1 ± ± ± 3.7 N: TP ± 0.6* 28.1 ± 2.0* D: Control 17.7 ± ± ± 1.9 D: TP ± 1.5* 21.4 ± 1.6* Glucose-6-phosphate (G-6-P) dehydrogenase N: Control 12.7 ± ± ± 3.1 N: TP ± 3.1* 27.1 ± 5.8 D: Control 15.7 ± ± ± 2.3 D: TP ± 1.7* 30.4 ± 8.0* A enzyme unit is defined as the amount that catalyzes the formation of each product per min. * Significant difference from the control at p < 0.05, by Student s t-test. * Normal mouse (N) and streptozocin-induced diabetic mouse (D) 1) Kiho, T., Morimoto, H., Kobayashi, T., Usui, S., Ukai, S., Aizawa, K., Inakuma, T. Effect of a polysaccharide (TAP) from the fruiting bodies of Tremella aurantia on glucose metabolism in mouse liver. Biol. Pharm. Bull., 64, (2000). 7

9 Ver. 1.3SJ 3-2. in vivo 1 % 0.5 ml Group (g) (mg/g ) Control n = (g) mg/g Control 0 Control g ± % 0.5 ml in vivo Kiho 2) Tremella sp. 0.5 g/l 2) Kiho, T., Morimoto, Kochi, M., T., Usui, S., Hirano, K., Aizawa, K., Inakuma, T.,Effect of an acidic polysaccharide (TAP) from Tremella aurantia and Its degredation product (TAP-H). Biol. Pharm. Bull., 24, (2001). 8

10 Ver. 1.3SJ 3-4. in vivo -P 50, 200 mg/kg 30 5 ml/kg mg/kg 2 4 (mg/dl) * (hr) Control 50 mg/kg 200 mg/kg 8. n = 6 ± *p < 0.05 * 18 ddy , 200 mg/kg 1 5 ml/kg 2,4 6 E- 9

11 Ver. 1.3SJ in vivo 3 4. Group Triglyceride (mg/dl) Control ± 12.4 (0.1 %) ± 25.4 (0.2 %) ± 15.4 Each value represents mean with the S.E. of 5-6 mice. 5 ddy CRF-1, 0.1 % 0.2 % in vivo Kiho 1) Tremella sp.tp TP50 mg/kg 5. TP Group Dose Relative plasma cholesterol level (mean ± S.E.) (n=15) (mg/kg) (h) Normal mice Control ± ± 2.4 TP ± 4.7* 91.7 ± 2.7 Diabetic mice Control ± ± 4.8 TP ± 7.9* 78.6 ± 2.7* Each plasma cholesterol level at 0 h normal mice (N=15) ± 3.9 mg/dl, diabetic mice (n=15) ± 8.2 mg/dl was adjusted to 100. * Significant difference from the control at p < 0.05, by Student s t-test. * Normal mouse (N) and streptozocin-induced diabetic mouse (D) 10

12 Ver. 1.3SJ 4-3. in vivo Kiho 2) Tremella sp.tp 4-2 TP 6. TP Group (n=4-5) Total bile acid (mmol/g feces) Total cholesterol (mg/g feces) Control TP 96 ± ± 4.3* 4.73 ± ± 0.13* Feces were gathered for one week after 8 weeks of the test. Each value represents the mean ± S.E.M. of 4-5 mice. Significatly different from the control : * p< P50 mg LDL TG 3 HDL 7. LDL mg/dl 131 ± ± 31.7 HDL mg/dl 61.3 ± ± 28.5 mg/dl 206 ± ± 27.9 TG mg/dl 121 ± ± 43.3 meq/l 0.52 ± ± * LDL mg/dl 70~139 HDL mg/dl 40~77 mg/dl 130~219 TG mg/dl 30~149 meq/l 0.13~

13 Ver. 1.3SJ 200 LDL 120 HDL mg/dl mg/dl mg/dl TG) mg/dl meq/dl TG 150 mg/dl 2 TG mg/dl 150 mg/dl mg/dl TG TG TG LDL 110 mg/dl 2 LDL TG LDL 12

14 Ver. 1.3SJ in vitro MRS Lactobacillus bulgaricus 1.25 % control 1.0 OD = /ml Control 0 8h 16h 24h 36h 48h 10. OD 5-2. in vitro MRS Bifidobacterium bifidum 1.25 % control OD = /ml OD 2 1 Control 0 8h 16h 24h 36h 48h

15 Ver. 1.3SJ P50 mg Clostridium (C.) perfringens 4 Bifidobacterium spp.lactobacillus spp CFU/g Escherichia coli CFU/g 10.5 Bifidobacterium spp. 6 Lactobacillus spp. 7 log CFU/g log CFU/g log CFU/g C. perfringens 8 Escherichia coli log CFU/g log CFU/g

16 Ver. 1.3SJ % % % 0.20% 0.30%

17 Ver. 1.3SJ in vitro Kim 3) TFPC12h neurite The neurite length is expressed as the mean S.E.M. of the individual cell (n=150) in each group. Separate measures of one-way ANOVA of the swimming time among the groups were followed by Scheffe test. ## p <0.01, ### p <0.001 vs.control (vehicle). 14. PC12h DFDMEM/Hams F12, 1:1PC CO 2 3) Kim. J.H., Ha.H.C., Lee.M.S., Kang.J.I., Kim.H.S., Lee.S.Y., Pyun.K.H., Shim.I. Effect of Tremella fuciformis on the neurite outgrowth of PC12h cells and the improvement of memory in rats. Biol. Pharm. Bull., 30, (2007). 16

18 Ver. 1.3SJ 7-2. in vivo Kim 3) Morris TF TF sham The mean values of the four trials per day for 6 d for each of the four groups are shown. Repeated measures of ANOVA for swimming time among the groups were followed by the LSD test. * p <0.05, ** p < 0.01 vs. sham; # p <0.05 vs. control. 15. TF sham2 mg/kgcontrol 100 mg/kg 400 mg/kg 14 TF-100TF-400Morris Water Maze Test 17

19 Ver. 1.3SJ in vivo Kiho 1, 2) Tremella sp.tp 0.05 %10 30 % 30 % mg/dl mg/dl

20 Ver. 1.3SJ in vivo 8n = 10 Group mm 2 Control 5.41 ± ± 1.49 p < % ml ml ml % ml *

21 Ver. 1.3SJ ph -P ph ph % ph 2 ph 4 ph 7 ph ph ph ph P B 200 [1/s] 25 ph 30 ph Pas Pas ph ph ph 20

22 Ver. 1.3SJ P (%)

23 Ver. 1.3SJ (LD50) n = 10-P1000 mg/kg14 P LD mg/kg n = 20-P227 mg/kg Ames TA98,TA100 -P Ames µg/plate n = 15-P1.25 g/kg P50 mg ASTALTLDγGTLDL HDL TIBCUIBC P

24 Ver. 1.3SJ 12. P 1.0 g/100 g g/100 g 5.6 g/100 g 0.0 g/100 g kcal/100 g g/100 g 2300 mg/100 g 5.8 g/100 g P 1 -P mg 14. ) () P *-P 1 % 23

25 Ver. 1.3SJ 15. -P () 5 kg <> P 24

26 Ver. 1.3SJ (Tremella fuciformis) 10~30 %80 %

27 Ver. 1.3SJ TEL(0586) () FAX(0586) URL/ F TEL(03) FAX(03) * * * * P

白キクラゲ多糖体 White Jelly Fungus 1. はじめにシロキクラゲは, 日本を始めとするアジア温帯地域の広葉樹林に見られる一般的な食用キノコですキクラゲと名前は付いていても, シロキクラゲはキクラゲとは生物学上全く別種のもので非常にやわらかく美しい白色透明の子実体を形成します

ORYZA OIL & FAT CEMICAL CO., LTD. 白キクラゲ多糖体 WITE JELLY FUNGUS Ver. 1.4KT 白キクラゲ多糖体 White Jelly Fungus 1. はじめにシロキクラゲは, 日本を始めとするアジア温帯地域の広葉樹林に見られる一般的な食用キノコですキクラゲと名前は付いていても, シロキクラゲはキクラゲとは生物学上全く別種のもので非常にやわらかく美しい白色透明の子実体を形成します