() ( 2 1)90 (2010) ( 1) QIAGEN DNeasy Blood & Tissue Handbook FAVORGEN Tissue Genomic DNA Extract
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1 2012 (Sekiya et al 2012) ( ) ( 1) 1
2 () ( 2 1)90 (2010) ( 1) QIAGEN DNeasy Blood & Tissue Handbook FAVORGEN Tissue Genomic DNA Extraction Mini Kit DNA (Lian et al 2006) 7 (4_109 4_114 6_167 6_234 6_603 6_571 4_472) (Polymerase Chain Reaction: PCR) 4.5 l 0.5 l DNA l Type-it Microsatellite PCR Kit Type-it Multiplex PCR Master Mix, 2x 2.5 l Q-Solution, 5x 0.5 l H2O 1.0 l PCR TaKaRa PCR Thermal Cycler Dice TM Version 3 Model TP650 DNA Applied Biosystems 3130xl Genetic Analyzers (He) ( 2013)He GenAlEx 6.2 (Peakall and Smouse 2006) 2
3 2 STRUCTURE v (Pritchard et al 2000) 22 () Population Graph (Dyer and Nason 2004) ( 3) ( m (730.4m m)) 10 (cm) ( ) 1 3
4 6 GIS Analysis (Arc GIS) (Topographic wetness index TWI) TWI m TWI (Sørensen and Seibert 2007) (2012) AIC 100m (Hanskii 1994) (42 17 ) () (Martin et al 2005) AIC Akaike weight 95 CI( ) GLM R 3.0.2(R core Team 2013) 4
5 1 (1) STRUCTURE STRUCTURE lnp(d) K=1 K=5 ( 4A) K K=5 ( 4B) K=6 ( 5) K=5 K=2 K=5 K=2 2 K=5 2 (2) (He) 1 He MK RO US (3) Population Graph (22 )( 6 7) 7 5
6 AIC 2 ( 2) AIC 2 AIC 95 0 ( 8) 0 ( 8) 30cm ( 9) ( 9) 6
7 1. (1) (MK RO SD) (OG TS) He He 3 STRUCTURE 3 (K=5 5) 3 (2) Population Graph ( 10) () 7
8 2. 6 TWI TWI TWI 3. STRUCTURE 3 3 8
9 1 2 ( 2010) 9
10 10
11 Dyer R, Nason J (2004) Population graphs: the graph theoretic shape of genetic structure. Mol. Ecol. Lian CL, Wadud MA, Geng Q, et al (2006) An improved technique for isolating codominant compound microsatellite markers. J Plant Res 119: doi: /s Martin TG, Wintle B a, Rhodes JR, et al (2005) Zero tolerance ecology: improving ecological inference by modelling the source of zero observations. Ecol Lett 8: doi: /j x Pritchard JK, Stephens M, Donnelly P (2000) Inference of Population Structure Using Multilocus Genotype Data. Genetics 155: Sekiya K, Miura I, Ogata M (2012) A new frog species of the genus Rugosa from Sado Island, Japan (Anura, Ranidae). Zootaxa 62: Sørensen R, Seibert J (2007) Effects of DEM resolution on the calculation of topographical indices: TWI and its components. J Hydrol 347: doi: /j.jhydrol ,,, &. (2012).. Japanese Journal of Conservation Ecology, 164,
12 1 n He ID n He (W) OG AH TS DM (K) (E) DMD SG KT KD NG(P) YY NG(R) ST MT US AT ON KB GS JK SS TY KG UM GZ MG HN MK RO SD
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15 Forest Paddy fields Salt water Others
16 LnP(D) (A) K (B) STRUCTURE (A)LnP(D) (B) K 16
17 K=2 K=3 K=4 K=5 K=6 5 STRUCTURE 1 17
18 6 Population Graph () 7 Population Graph 9 18
19 2 AIC2 19
20 8 20
21 (/km) (A) (/km) (B) 9 (A) (B) 21
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