論文

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1 *, 1 1 Selection of superior Frankia spp. for rapid cultivation of Alnus hirsuta seedlings Maki Saito *, 1 and Mikio Hasegawa cm 30 cm 70 % 5 Abstract: In order to develop the rapid cultivation of potted seedlings of Alnus hirsuta, we selected Frankia spp. favorable for the growth of A. hirsuta. The growth of A. hirsuta seedlings inoculated with suspension of root nodules of the species for two years after germination was different among the root nodule suspensions derived from natural seedlings collected from different four sites. Using the root nodule suspensions re-derived from the seedlings with the best and worse growth-performances revealed by the experiments, repeatable results were obtained, where the average size of the 28 potted seedlings with the best growth-performance exceeded 30 cm in the middle of September and the proportion of seedlings higher than 30 cm was about 70 %. This study demonstrated that A. hirsuta seedlings grew to the size suitable for shipping, for about five months, by the inoculation of Frankia spp. with high growth promoting effects as selected in this study, into germinated seedlings. Keywords: labor saving, local planting stocks, low cost, potted seedlings, revegetation plants Alnus hirsuta Frankia , 1994 Yamanaka et al DNA * saito@fes.pref.toyama.jp 1 Forestry Research Institute, Toyama Prefectural Agricultural, Forestry & Fisheries Research Center, 3 Yoshimine, Tateyama-machi, Nakaniikawa-gun, Toyama , Japan

2 cm mg/l 440 mg/l 56 cm 27 cm 25 cm A B C D cm cm 27 cm 25 cm cm g/l 1 2mL mg/l 1 A 690 m B 810 m C 1010 m D 1100 m 1180 m 1010 m 670 m 1100 m g/l ml cm 700 ml 8

3 A B A 1% B 5% 2 % F P -9 ** n.s n.s ** 1 % n.s. 9

4 cm 30 cm 69.2% cm cm 3 30 cm % A 2 B 6 1 A 1 B C % Rhizobium 10

5 Schubert et al Rhizobium cm 30 cm 1 30 cm cm mm mm ph Rhizobium pf pf

6 : : : : ,.,, pp : : : : (1982) Fusarium oxysporum. 22: Schubert KR, Jennings NT, Evans HJ (1978) Hydrogen reaction of nodulated leguminous plants. II. Effects on dry matter accumulation and nitrogen fixation. Plant Physiology 61: En-b : : ,.,, pp Yamanaka T, Akama A, Li C-Y, Okabe H (2005) Growth, nitrogen fixation and mineral acquisition of Alnus sieboldiana after inoculation of Frankia together with Gigaspora margarita and Pseudomonas putida. Journal of Forest Research 10: : USDA. 64: :

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