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1 , Kaoru ARIYAMA 10 17N, Na, Mg,Al,Si,P,S,Cl,K,Ca,Fe,Cu,Zn,Se,Br,Rb Sr 14r Tricholoma matsutake,,, JAS ICP-OESICP-MS 2ICP-OES ICP-MS,, Tricholoma matsutake -24-
2 ,, 2007,, 10 17,,,,,,, 14 44, 22, 22, 28, 15, 5 25 g ICP-OES ICP-MS 2-25-
3 Ariyama, SRMNational Institute of Standard & Technology Gaithersburg, MD, USA Wheat FlourSRM1567aRice FlourSRM1568aSpinach Leaves SRM1570aApple LeavesSRM1515Tomato LeavesSRM1573aPine NeedlesSRM1575a Corn KernelRM8413China National Analysis Center for Iron and SteelBeijing, China TeaGBW07605CabbageGBW08504Bush Branches and LeavesGBW
4 -27-
5 -28-
6 B-400 FC-610 MP XRF MilliQ Element 18 MΩcm 1cm 56 g g 35 mm 30 t 20 5mm 30 mm TiN, Na, Mg, Al, Si, P, S, Cl, K, Ca, Fe, Cu, Zn, Se, Br, Rb Sr SRM Bush Branches and Leaves BG 2θ a n b r 2 (s) (s) (deg) N Kα c raw % Na Kα raw µg/g Mg 10 5 Kα Net % Al Kα raw µg/g Si Kα Net % P 10 5 Kα raw % S 10 5 Kα Net/BG % Cl Al Kα raw % K 10 5 Kα raw % Ca 10 5 Kα Net % Fe Kα Net/BG µg/g Cu Kα Net/BG µg/g Zn Kα Net/BG µg/g Se Kα Net µg/g Br Kα Net/BG µg/g Rb Kα Net µg/g Sr Kα Net/BG µg/g a BG1 BG2 b c raw d 0 e Net -29-
7 STATISTICA Pro 06J Micrisoft Excel 2002Micrisoft corporation, Redmond, WA, USA Welch t Micrisoft Excel fold cross validation raw NetNet Net BG SRM NetNet BG 0 r Net BG r Net BG Fe 3 r Net BG Net r NetBG Na Fe 3 r Net BG r raw SRM SRM N S 3 r r N Net S Net BG Mg rawnet r Net BG r Net 0 P Net BG r raw Net BG r BG BG1 BG2 BG -30-
8 Ca Net BG raw Net r Net 0 Cl K raw Net BG BG1 BG2 raw BG raw BG1BG2 17 N, Na P r SRM Net raw BG1 BG BG2 (a) raw (b) Net (c) Net /BG Fe 3 (a-1) raw (a-2) raw (b) Net (c) Net / BG Na 3-31-
9 (a) raw (b-1) Net (b-1) Net (c) Net / BG Mg 3 (a) raw (b-1) Net (b-1) Net (c) Net / BG Ca 3 SRM Bush Branches and Leaves 7 repeatability 5 reproducibility Se repeatability 7 reproducibility 9 SRM Bush Branches and Leaves repeatability reproducibility repeatability reproducibility (%) (%) N 1.5 % Na 1.96 % Mg 0.48 % Al 2000 µg/g Si 0.60 % P % S 0.73 % Cl 1.92 % K 0.92 % Ca 1.68 % Fe 1070 µg/g Cu 6.6 µg/g Zn 55 µg/g Se 0.12 µg/g Br 3.0 µg/g Rb 4.5 µg/g Sr 246 µg/g
10 ICP-OES ICP-MS 2 Mg P Mg SRM P SRM ICP-OES ICP-MS,, 10 Na Mg Na Mg P SRM Fundamental Parameter FP ICP ICP ICP N % Na µg/g Mg % Al µg/g Si ND % P % S % Cl % K % Ca % Fe µg/g Cu µg/g Zn µg/g Se ND ND µg/g Br µg/g Rb µg/g Sr ND 0.68 µg/g -33-
11 10 ICP ICP ICP ICP ICP N ND 0.5 % Na µg/g Mg % Al ND ND ND ND µg/g Si ND ND ND ND % P % S % Cl % K % Ca % Fe ND ND ND ND µg/g Cu ND 3 ND 5 µg/g Zn µg/g Se ND ND ND ND µg/g Br µg/g Rb µg/g Sr µg/g n=44 n=22 n=22 n=28 n=15 n=4 Mean SD Mean SD Mean SD Mean SD Mean SD Mean SD N ** % Na µg/g Mg ** ** % Al ** ** µg/g Si ** ** ** % P ** ** ** % S ** % Cl ND ** % K ** % Ca ** ** ** % Fe ** ** ** ** µg/g Cu ** ** 19 4 µg/g Zn ** µg/g Se ** ** ** µg/g Br ND ** ND ** µg/g Rb ** µg/g Sr ND ND ND µg/g ** 1% -34-
12 ,,, 4 4Mg, Si, P, K, Ca Cu ,, Mg, Si, P, K, Ca Cu fold cross validation fold cross validation , 64, n crossvalidation crossvalidation Al, Si, P, K, Zn Na,P,K,Ca,Se,Rb N, K, Ca
13 5g 0.3 g FP 10 17N, Na, Mg,Al,Si,P,S,Cl,K,Ca,Fe,Cu,Zn,Se,Br,Rb Sr 14 r2 0.96,, Mg, Si, P, K, Ca Cu 6 10-fold cross validation Ariyama, K; et al, J. Agric. Food Chem., 55,
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