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1 GE imagination at work

2 Typhoon Typhoon Molecular Dynamics 1989 PhosphorImager 1992 CLSM 1

3 View it all from your benchtop 2000 Gen III System 1998 MegaBACE 1995 STORM 1993 FluorImager 2

4 Storage Phosphor Autoradiography RI PhosphorImager Typhoon 1 10 DNA Typhoon cm Typhoon 25 µm MCID Analysis ❶ BaFBr:Eu 2+ ❷ ❸ ❹ 2 RI Typhoon

5 TyphoonKodak cm 1 ImageEraser Imaging Plate 33 P CLONTECH Atlas array Typhoon Typhoon 3 H 14 C 32 P 35 S 125 I 10 5 Typhoon LOG (Typhoon SIGNAL) O.D. 3 O.D. 2 O.D. 1 O.D LOG (DISINTEGRATIONS/MM 2 ) Typhoon Film OPTICAL DENSITY 4

6 Fluorescence & Luminescence Typhoon nm TLC nm 10% DNA Vistra Green Typhoon TyphoonSYBR Green Band Shift FAM HEX DNA Mnt Protein Band Shift4 DNA5 8 Washington University, School of Medicine, Department of Genetics, Cris Man ECF Substrate AttoPhos NonRI ECF SubstrateDDAO-phosphate ECL Plus Typhoon tublin ECL Plus Substrate Typhoon PMT 5

7 Reason... 3 mm 10 1,000 µm 10 µm EGFP-F 6

8 Typhoon FluorSep Molecular Dynamics ECF nm HRP 7

9 Applications Typhoon Typhoon µm TyphoonCy3 Cy5 SYPRO Ruby Typhoon Ettan DIGE 13 DNA AFLPTyphoon Typhoon FluorSep ImageQuant 1 4 JIRCAS 8

10 Analysis ArrayVision Array Analysis ArrayVision ArrayVision ArrayStat ImageMaster 2D ImageMaster 2D 2 2D PAGE Analysis ImageMaster 1D DNASDS-PAGE IEF- PAGE Fragment Analysis 9

11 Typhoon 9410 Typhoon 4 10 µ Typhoon 9210 Typhoon µ Typhoon Typhoon 9400 Typhoon Cy2 Cy3 Cy5SYPRO Ruby FITC ECL Plus Typhoon 9200 Typhoon PMT Typhoon 10

12 Selection Typhoon ImageQuant ImageQuant Typhoon Typhoon FluorSep ImageMaster 2D ImageMaster 1D 11

13 Upgrading Typhoon Typhoon , 633 nm Typhoon , 633 nm Typhoon Typhoon , 488, 532, 633 nm Typhoon , 488, 532, 633 nm Ettan DIGE Typhoon 9400 / Cy2,Cy3,Cy / 9210 / Cy3,Cy5 Ettan DIGE ArrayVison ArrayStat MCID Analysis ArrayVison Study type 12

The Difference in Protein Expression Ettan DIGE 2D DIGE Ettan

DIGE CyDye DIGE fluors DeCyder Typhoon ImageMaster 2D

14 The Difference in Protein Expression Ettan DIGE 2D DIGE Ettan DIGE Typhoon 10 % Ettan DIGE 2D DIGE Typhoon 2D DIGE Ettan DIGE CyDye DIGE fluors DeCyder Typhoon ImageMaster 2D Percentage within D DIGE / DeCyder 2D DIGE / / Ettan DIGE DeCyder 2D DIGE % Ettan DIGE 2D DIGEDeCyder 95 % Ettan DIGE

Acquisition Typhoon12 Typhoon Image Analysis Typhoon DeCyder DeCyder Software 2D DIGE

15 Ettan DIGE Sample Labeling Sample1 Sample2 Sample3 Cy2 Cy3 Cy5 Protein Separation 2D PAGE Ettan DALTsix Electrophoresis Unit Ettan IPGphor Isoelectric Forcusing Unit Image Acquisition Typhoon12 Typhoon Image Analysis Typhoon DeCyder DeCyder Software 2D DIGE DeCyder DeCyder DIA: difference in-gel analysis BVA: biological variation analysis 2 DIATyphoon Cy3 Cy5 BVA 14

Accessories Typhoon Unmounted,LE 35 43 cm Screen & Cassette 63-0033-55 Unmounted,LE 20 25 cm Screen & Cassette 63-0033-57 Unmounted,GP 35 43 cm Screen & Cassette 63-0034-79 Unmounted,GP 20 25 cm

16 Accessories Typhoon Unmounted,LE cm Screen & Cassette Unmounted,LE cm Screen & Cassette Unmounted,GP cm Screen & Cassette Unmounted,GP cm Screen & Cassette Tritium Screen cm Unmounted Screen Exposure Cassette for Unmouted Screen cm Kapton Tape Roll WonderWedge MicroArray Slide Holder Fluorescence Imaging 72-HB HB01-01 ECL Plus Western Blotting Starter Kit Full ECL Plus Western Blotting Detection Reagents ECF Random-Prime Labeling Module ECF Random-Prime Labeling and Detection System ECF Western Blotting Kit ECF Western Blotting Reagent Pack for Mouse Antibody ECF Western Blotting Reagent Pack for Rabbit Antibody ECF Substrate Gene Images 3 -Oligolabeling Module 3 -Oligolabeling and ECF Signal Amplification System Vistra Green Nucleic Acid Stain µl 72-AS00-41 RPN2132 RPN5751 RPN5752 RPN5780 RPN5781 RPN5783 RPN5785 RPN5770 RPN5775 RPN5787 Option Software ImageQuant satellite licence ArrayVision ArrayStat MCID Analysis ImageMaster 1D ImageMaster 2D MCID Analysis 15

Specification Typhoon9210 9200 Typhoon9410 9400 Typhoon 9200 Typhoon

633 nm Ar 457,488 nm 25, 50, 100, 200 10, 25, 50, 100, 200 25, 50, 100,

1,000 µm Storage Phosphor Screen Kodak Imaging Plate 35 43 cm 10 µm35 14

17 Specification Typhoon Typhoon Typhoon 9200 Typhoon 9210 Typhoon 9400 Typhoon 9410 SYAG 532 nm SYAG 532 nm HeNe 633 nm HeNe 633 nm Ar 457,488 nm 25, 50, 100, , 25, 50, 100, , 50, 100, , 25, 50, 100, , 1,000 µm 500, 1,000 µm 500, 1,000 µm 500, 1,000 µm Storage Phosphor Screen Kodak Imaging Plate cm 10 µm35 14 cm 1 100,000 65,53616bit Windows / 230 V cm H W D 160 kg 16

2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE) technology is covered by US patent numbers US6,043,025 and US6,127,134 and foreign equivalents and exclusively licensed from Carnegie Mellon

18 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE) technology is covered by US patent numbers US6,043,025 and US6,127,134 and foreign equivalents and exclusively licensed from Carnegie Mellon University. CyDye: this product or portions thereof is manufactured under licence from Carnegie Mellon University under US patent number US5,268,486 and other patents pending. Some of these products may only be available to collaborators and customers within certain of our technology access programmes. The purchase of CyDye DIGE Fluors includes a limited license to use the CyDye Fluors for internal research and development, but not for any commercial purposes. A license to use the CyDye Fluors for commercial purposes is subject to a separate license agreement with GE Healthcare.

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Unknown Cell Sorting S3e EVOLUTION 1 STREAMLINED 2 PRINCIPLE 3 HIGH PERFORMANCE 4 AUTOMATION 5 6 INNOVATIVE 7 8 VERSATILE 9 INTUITIVE 10 BIO SAFETY 11 BioSafety Air in Air out Air in Air in System airflow 12 SOLUTIONS