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1 ORYZA POLYAMINE ORYZA POLYAMINE -P -PC -LC 1. Ver.1.0 MM

2 ver. 1.0MM 1) Roseeuw D.I. et al., Epidermal keratinocytes actively maintain their intracellular polyamine levels. Cell Tissue Kinet. 16, (1983). 2) Ha.C. et al., The natural polyamine spermine functions directly as a free radical scavenger. Proc. Natl. Acad. Sci. USA 95, (1998). 3) Fujisawa S. et al., Kinetic evaluation of polyamines as radical scavengers. Anticancer Res. 25, (2005). 4) Soda K. et al., Spermine, a natural polyamine, suppresses LFA-1 expression on human lymphocyte. J. Immunol. 175, (2005). 5) Zhang M. et al., Spermine inhibits proinflammatory cytokine synthesis in human mononuclear cells:a counterregulatory mechanism that restrains the immune response. J. Exp. Med. 185, (1997). 6) Gugliucci A. et al., The polyamines spermine and spermidine protect proteins from structural and functional damage by AGE precursors: a new role for old molecules?life Sci. 72, (2003). 7) Gugliucci A., Alternative antiglycation mechanisms: are spermine and fructosamine-3-ki nase part of a carbonyl damage control pathway? Med. Hypotheses (2005). 8) Soda K., Polyamine intake, dietary pattern, and cardiovascular disease. Med. Hypotheses. 75, (2010). 9) De la Pena N.C. et al., Inhibition of platelet aggregation by putrescine, spermidine, and spermine in hypercholesterolemic rabbits. Arch. Med. Res.75, (2000). 1

3 ver. 1.0MM 10)Ramot Y. et al., Polyamines and hair: a couple in search of perfection. Exp. Dermatol. 19, (2010). 11) Ramot Y., Spermidine promotes human hair growth and is a novel modulator of human epithelial stem cell functions. Plos One, 6,1-11(2011) H N NH H 2 N 2 H 2 N N H H N NH2 H 2N NH 2 1. ODC 2 12,13) 3 14) 4 2

4 ver. 1.0MM ODC S

5 ver. 1.0MM 12) Yoshinaga K. et al., Age-related changes in polyamine biosynthesis after fasting and refeeding. Exp. Gerontol. 28, (1993). 13) Scalabrino G. et al., Polyamines in mammalian ageing: an oncological problem, too? A review. Mech. Ageing Dev. 26, (1984). 14) Soda K. et al., Long-term oral polyamine intake increases blood polyamine concentrations. J Nutr. Sci. Vitaminol. 55, (2009)

6 ver. 1.0MM Atiya A. M. et al., Polyamines in foods: development of a food database. Food Nutr. Res. 55,3402 (2011) = (diamine oxidase) 5

7 ver. 1.0MM 6

8 ver. 1.0MM 4. (1) 7

9 ver. 1.0MM 8 40~60 % / ( 0.33 %) µg/ml 100 µl 24 MTT Assay p0.01 p0.05 mean±sen=3) (µg/ml ) 8

10 ver. 1.0MM / ( 0.33 %) µg/ml 500µL 1~2 Total RNA c DNA PCR (2) 9

11 ver. 1.0MM 10. (3) () % % 11 10

12 ver. 1.0MM % 1,3- ph () :0.33 % (%) %

13 ver. 1.0MM (4) Normal ( 5 ) ( 6 ) ( 7 ) ( 8 ) ( 9 ) (10) (11) (12) 12

14 ver. 1.0MM ( 13) ph 1 ( 14). 13

15 ver. 1.0MM 7. -P 50 ~ 100 mg / 8. -P 100 g 6.3 g 9.0 g (6.25) 1.0 g 34.4 g 40.8g 100g (+++) 225 kcal 8.5g 68.8mg * 4; 9; 4; 2 -P : No (1)

16 ver. 1.0MM (2) (LD 50 ) 30 g 5 ICR, 2,000 mg/kg 23±2 50±10% 14 LD 50 2,000 mg/kg (3) Salmonella typhimuriumta100ta98 Ames 19.52,500 µg/plate P () 5 kg -PC () 5 kg -LC ) 5kg

17 ver. 1.0MM 13. P INCI PC ORYZA SATIVA (RICE) EXTRACT DEXTRIN CITRIC ACID LC BG WATER BUTYLENE GLYCOL ORYZA SATIVA (RICE) EXTRACT CITRIC ACID 16

18 ver. 1.0MM 0.2 % (HPLC ) 10.0 % (1 g1052 ) (1) 20 ppm () Pb (2) 1 ppm ( 3 B ) As 2 O g () g ( ) (BGLB ) 53 % 30 % 17 % 100 % 17

19 ver. 1.0MM 0.2 % (HPLC ) 10.0 % (1 g1052 ) (1) 20 ppm ( 2 ) Pb (2) 1 ppm ( 3 ) As 2 O g () g ( ) (BGLB ) 53 % 30 % 17 % 100 % 18

20 ver. 1.0MM % (HPLC ) (1) 10 ppm ( 2 ) Pb (2) 1 ppm ( 3 ) As 2 O g () g ( ) (BGLB ) % 10 % 0.65 % 0.21 % 100 % 19

21 ver. 1.0MM OEM TEL(0586) () FAX(0586) URL/ F TEL(03) FAX(03) * *

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untitled POLYAMINE -P Ver.1.0 MM ver. 1.0MM POLYAMINE 1. 1) Roseeuw D.I. et al., Epidermal keratinocytes actively maintain their intracellular polyamine levels. Cell Tissue Kinet. 16, 493-504 (1983). 2) Ha.C. et

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