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5 マ ウ スの病 態 生 理 に 関 す る実 験 的 研究 表4 従 つ てCa排 泄 量 は,対 痙 攣 群 で 約60%多 く,ま 尿17-Hydroxycorticosteroidl 照 のCF-1系 マウスより た 未 痙 攣 群 よ り約7.5%痙 に 述 べ た 方 法 に 従 つ て 総 量 遊 離 型 結 合 型 に わ け て 得 た 成 績 を 示 せ ば 表4の お, マ ウ ス:痙 攣 群 で は 平 均 値: 4.74μg 揺 範 囲 μg/ml.未 群 で は 平 均4.70μg/ml,動 痙攣 揺 範 囲 μg/ml. CF-1系 マ ウ ス 平 均 値4.92μg/ml,動 揺 範 囲 μg/ml. 遊離型 /ml,動 マ ウ ス:痙 攣 群 で は 平 均 値2.32μg 揺 範 囲 μg/ml.未 群 で は 平 均 値2.31μg/ml,動 痙攣 揺 範 囲 μg/ml. CF-1系 マ ウ ス:平 均 値2.38μg/ml,動 揺 範 囲 μg/ml. 結合型 マ ウ ス:痙 /ml,動 攣 群 で は 平 均 値2.42μg 揺 範 囲 μg/ml.未 群 で は 平 均 値2.39μg/ml,動 マ ウ ス:平 痙攣 揺 範 囲 μg/ml. CF-1系 如 くで マ ウ ス:痙 未 図1 総量 合 型(C)と 遊 離 型(F)の 攣 群 で は 平 均 値1.06,動 痙 攣 群 で は 平 均 値1.04,動 マ ウス は痙 攣 群 と 未 痙 攣 群 と に わ け て し ら べ た. /ml,動 な お,結 比(C/F) C/F 尿 中17-Hydroxycorticosteroid 第2章,第5節 あ る.な 揺 範 囲 μg/ml. につ いて み るに 攣 群 に 多 い. 第3節 183 均 値2.53μg/ml,動 尿 ア ミ ノ 酸( マ ウ ス) 揺範囲 揺範囲

6 184 杉 図2 尿 ア ミノ 酸(CF-1系 生 マ ウ ス) 了 亮 量, serine+glycineは1/2量, acidは2/3量, 量 で, glutamic leucine+isoleucineは1/4 alanineお よ びvalineは ほ とん ど 同 量 で あ つ た. 第4章 総 括 な ら びに 考 按 マ ウ ス の 尿 成 分 を 対 照 のCF-1系 マ ウ ス の 尿 成 分 と 比 較 す る に,ま ず一般 検 査 に お い て ほ と ん ど 差 が な く,ま 解 質 に お い て もNa, K, に は 大 差 が な い が,た み 対 照 の 約60%高 ら に こ れ を Clお だCaに お いての い 値 を 示 して い る,さ マ ウス の痙 攣 群 と未痙 攣 群 に わ け て 測 定 し て み た と こ ろ,痙 群 で は 未 痙 攣 群 の7.5%の れ た.ま た電 よ びMg 攣 増加 が認 め ら た 尿 中17-OHCSも 総 量,遊 離 型 お よ び 結 合 型 と も対 照 お よ び 未 痙 攣 群 と 差 が み られ な か つ た.尿 に お い て もpaper 中amino chromatogaaphyは 照 の もの と 同 一patternを acid 対 示 し て お り, 量 的 に も有 意 の 差 は 見 出 しえ な か つ た. 実 験 成 績 の 項 で は か な り の 差 が み られ た 表 CF-1系 マ ウ ス:平 均 値1.06,動 揺 範 囲 以 上 の 如 く に 尿 中17-OHCSは マ ウス の痙 攣 群 お よ び 未 痙 攣 群 と 対 照 のCF-1系 総 量,遊 離 型,結 マ ウス の間 に 合 型 お よ びC/Fに ほ とんど差が み ら れ な か つ た. 第4節 尿amino 第2章,第6節 acid に 記 載 の 方 法 に 従 い の 尿 中 に 排 泄 され るamino 成 績 を 示 せ ば 図1の threonine, acid, arginine, glycine, tyrosine, aspartic acid, cystein, isoleucine, valineら 他 確 認 し て な い が,文 methylhiatidineの leucine, serine, が 検 出 さ れ る.そ の 献 か ら 考 え て β-alanine, 位 置 にspotが これ を 対 照 のCF-1系 graphyと マウス 二 次 元展 開 し た 如 く で あ る. す な わ ち, alanine, glutamic acidを 認 め られ る. マ ウ ス のpaper 比 較 す る と(図2), が ほ と ん ど 同 一 のpatternで spotの あ り,と chromato 大 小 は あ る くに注 目すべ き 変 化 は 認 め ら れ な か つ た. な お,一 量 し た.そ 部 の も の は 標 準 液 と570mμ の 成 績 を 示 せ ば 表5の 如 く, aspartic acidは にて 比 色定 如 く で あ る.表 対 照 のCF-1系 の マ ウ ス の2倍 尿 ア ミ ノ 酸

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8 2) Naruse, H., Kato, M., Kurokawa, M., Haba, R. land Yabe, T.. J. Neurochem., 5, 359,

9 ) Kurokawa, M., Kato, M. and Machiyama, Y.: Biochem. Biophys. Acta, 50, 385, ) Butrer, A. M. and Tuthill: J. Biol. Chem., 93, 171, ) Hoffmann, W. S.. J. Biol. Chem., 120, 57, ) Schwartzenbach, G. und Ackermann, H.. Helv. Chim. Aceta., 30, 1798, ) Sobel, A. E. et al: Proc. Soc. Exp. Biol. & Med., 77, 737, ) Lefheiz, H. C.. J. Lab. & Clin. Med., 47, 623, ) Sharp, F. R., Elliott, T. & Leuris: Clin. Chem., 5, 488, ) Kolthoff, I. M.. Biochem. Z., 185, 384, ) Heagy, F. C.. Canad. J. Res., (Sec. E.), 26, 295, ) Orange, M. and Rhein, H. C.. J. B. C., 189, 379, ) Silber, R. H. and Poiter, G. C.. J. Biol. Chem., 210, 923, ) Moore, S., Spackmann, D. H. and Stein, W. H.: Anal. Chem., 30, 1185, ) Kay, R. E., Haris, D. C. and Entenman, C.: Arch. Biochem. Biophys., 63, 14, ) Golfer, M. F. and Essex, H. E.. Am. J. Physiol., 150, 27, ) Flesch, J.: Neurol. Centralbl., , ) Lennox, W. G. and Allen, M. B.: Arch. Neur. and Psychiat., 24, 1199, ) McQuarrie, I.: Am. J. Dis. Child., 72, 472, ) Bigwood, E. J.: Arch. Neur. and Psychiat., 24, 1199, ) Fletscher, R. T., Glasy, M. B. and Peden, O. D.: Lancet, 228, 1382, ) Griffiths, G. M.: J. Neur. and Psychopath., 15, 29, ) Scott, M. and Pigott, A. W.: Arch. Neur. and Psychiat., 36, 590, ) Belford, J. and Bonnycastle, D. D.: Arch. Neur. Psychiat., 64, 797, ) Hirschfelder, A. D. and Haury, V. G.: J. A. M. A., 102, 1138, ) Marco, C.. Acta Paediat. Latina, 10, 216, ) Lowell, F. C., Franklin, W., Beale, H. and Schiller, I. W.: New Engl. J. Med., 244, 49, ) Geppert, L. J., Dietrick, A. C., Johnston, E. H. and Lind, C. J.: Amer. J. Die. Child., 84, 416, ) Wayne, H. L.: J. Clin. Endocrin., 14, 1039, ) Stephen, E. H. M. and Noad, K. B.; Med, J. Aust., 2, 334, ) Pine, I., Engel, F. L. and Schwartz, T. B.: Electroenceph. Olin. Neurophysiol., 3, 301, ) Glaser, G. H.. Epilepsia, 2. 7, 1953.

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