その科学と技術 No.48( )

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1 45 CI +,,* a-, +0 a-, +0 a-, +., a-, + +, - +, CD CF - CTS CD a-cd b-cd g-cd a- +., CI CI CD CD a- +., CI a- +0, CD Bacillus circulans T--*.*. 13 a-, +0 CI-1 CI-2. CI-3 +* / CI 0 CI-1 CI CI

2 46 + CI-1 b-cd CI 1 CD + 1 CI-1 1 b-cd CI CD CI CI CD CI 1 CI CI,**2 CI-+* CI,,***.* Amide-2* Tosoh HPLC a- +0, a-, +- ao a-, + 0.

3 47 +-, CI-1 +1 C NMR A B C NMR CI-1 +1 C NMR +- +-, A, B, A CI-1 CI-+0 a-, +0, B CI * ++ MH, CI-+* ++ +, / +0 M, H CI-+1, M, Na CI ,30 +./2 +0,* +12, +3..,+*0,,02,.-*,/3,,1/.,3+0 + CI A B CI-1 +0 a- +, 0 CI-+1 NMR CI. CI Bacillus circulans T--*.* B. circulans U-+// Bacillus sp. --* K -/* K -0* K * K Paenibacillus sp. /3+ K /32K 2 CI CI - B. circulans T--*.* CI-1 CI-2 CI-3 3 CI-2 Paenibacillus sp. /32K CI-1 CI-2 CI-3 CI-1 Bacillus sp. --* K CI-2 CI-+* CI-++ CI-+, -/* K -0* K

4 48 - CI HPLC a:ci-1 b:ci-2 c:ci-3 d:ci-+* e:ci-++ f:ci-+, g:ci-+- h:ci-+. i:ci-+j:ci-+0 k:ci-+1 20* K Paenibacillus sp. /3+ K CI-1 CI-2 CI-3 0 CI-+* CI-++ CI-+, CI CI cy- cloisomaltooligosaccharide glucanotransferase, CITase - -+ CI + a-cd b-cd g-cd +* +../ +. 2/,-., CD CD CI 3

5 49. -,. Streptococcus mutans S. sobrinus CI 1 1 S. mutans in vitro /* mm 1 CI-2 / mm 1/ ++ *+. +* *+. *22. mm CI-1 CI CI-1 **,. *+. CI /0 S. mutans CI ++ CI CI-1 CI-+, S. mutans ph //. ph 1*. CI-1 CI-+pH / / CI

6 50 / CI- 1, CI- 2, CI- 3, CI- +*, CI- ++, CI-+, Streptococcus mutans CI ++ CI CI -- CI CD CD CD CI CD CI-1 CI-2 CI-3 2 CD CI-+* CI CI-1 CI-+, 0 CI +** mm ph 1.* *.*-, mm CI CI-1 CI-2 CI-3 CI-+* CI-++ CI-+, Glu a-cd b-cd g-cd +,. mm 0,/ 0,* nm a-cd *-.

7 51 0 +* CI-+*,, /* b-cd / g-cd CI +1 ao+,.o +, CI-+* CI-+* CI-++ CI-+, CI CD CI CI CI 2 -. CI a- +0, CI CI CI CI

8 52,* 0/ CI +, g. g + 3,0-2 CI CI +- ODS C Amide-2* Tosoh HPLC 1 CI-1 CI-2 CI-3 CI-+* CI-++ CI- +, a-, HPLC

9 53 a-, +- ao CI a-, +0 CI CI-1 CI-2 CI-3 2 CI +- CI 2 CI-1 2 3

10 54..+ CI CI CI 3 Leuconostoc a-, a-, +0 Bacillus CITase 3 CI,, CI CI., CI CI CITase a- +0, CI CI a-, +0 Leuconostoc mesenteroides NRRL 3

11 55 +* CI CI CI-1CI-2 CI-3 +. B-/+, F a- +, 0 3/ CI, +* L. mesenteroides NRRL B-/+, F CI Leuconostoc +/ sp. S-/+ S-/+ + B-/+, F a- +, 0 B-/+, F / +* ph */. +/ S-/+ a-, +0.- CI CI

12 56 ++ CITase CITase ,, DNA CITase B. circulans T--*.* CITase CITase ++*, - CITase.. CI CI CI CI CI CI

13 57 + CI CI CI-13 CI-+* +, IG-, 1 A B C D E ++43,-4* * /41,24- *43,,42 +04*, , * *41 +*4* +*41,43 *4. IG :,* / CI CI CI-1CI-3 CI, CI-+* CI-+, CI IG-, IG-1 +,* CI C CI CI +** WV CI + CI A B C CI S. mutans S. sobrinus +, M /* CI CI */ * ** +, CI CI CI E S. mutans S. sobrinus CI CI CI,*

14 58 +, AE WP Streptococcus sobrinus, S. mutans : : -* CI CI mix CI-1 CI- +, +... ww CI mix CI-+* +.. ww CI mix+* -* /* wv CI ,. 1,. CI- +* *+.. *20. mm *.,.,/2. mm *1..-,. mm +- CI CI

15 59 +. A: CI B : CI-1 CI-2 CI-3 CI-+* CI-++ CI-+, CI C: A 0 +* CI CI-+* 0 1,, /* +., mm CI-+* CI CI-+* CI-+* CI b-cd CI CD CI CD b-cd CI CI CI CI / + CI E CI,**2 CI,**3,+

16 60 CI-1 CI-2 CI-3 CI-+* CI-++ CI-+,,**3 CI +. CI CI CI CI + EB. Tilden and CS. Hudson, J. Am. Chem. Soc., 0-,,3**,3*, ( +3-3)., M. Kawamura, T. Uchiyama, T, Kuramoto, Y. Tamura and K. Mizutani, Carbohydr. Res., +3,, 2-3* ( +323). - G.L. Côté and P. Biely. Eur. J. Biochem.,,0, ( +33. ).. T. Oguma, T. Horiuchi and M. Kobayashi, Biosci. Biochem. Biotechnol., /1, +,,/ +,,1 ( +33-). / K. Funane, K. Terasawa, Y. Mizuno, H. Ono, T. Miyagi, S. Gibu, T. Tokashiki, Y. Kawabata, YM. Kim, A. Kimura and M. Kobayashi, J. Biotechnol., +-*, , (,**1). 0 K. Funane, K. Terasawa, Y. Mizuno, H. Ono, S. Gibu, T. Tokashiki, Y. Kawabata, YM. Kim, A. Kimura and M. Kobayashi, Biosci. Biotechnol. Biochem., 1,, -,11-,2* (,**2). 1 M. Kobayashi, K. Funane, T. Oguma, Biosci. Biochem. Biotechnol., /3, / ( +33/ ). 2 T. Oguma and H. Kawamoto, Trends Glycosci. Glycotechnol., +/, (,**-). 3 T. Oguma, K. Tobe and M. Kobayashi, FEBS. lett., -./, +-/ +-2 ( +33. ). +*,*** pp GTF p,+*,,/ , T. Takaha, M. Yanase H. Takata, S. Okada and SM. Smith, J. Biol. Chem.,,1+,,3*,,3*2 ( +330).

17 61 +- J. Appl. Glycosci., /.,1-*,**1 +. EJ. Hehre, Adv. Enzymol., ++,,31--1 ( +3/+ ). +/ K. Funane, T. Matsuo, H. Ono, T. Ishii, S. Gibu, T. Tokashiki and Kobayashi, M., J. Appl. Glycosci., /*, -13-2, (,**-). +0 T. Oguma, T. Kurokawa, K. Tobe and M. Kobayashi, J. Appl. Glycosci.,.,,.+/.+3 ( +33/ ). +1,**1 +233*/,**1. 2., ,**0 +3 K. Ochi, S. Okamoto, Y. Tozawa, T. Inaoka, T. Hosaka, J. Xu and K. Kurokawa, Adv. Appl. Microbiol., /0, +// +2. (,**.).,*.3,3-1,**0,+ FFI,+- +**+ +**2,**2

Correlation between molecular evolution of P450s and evolution of plant secondary metabolism Masaharu Mizutani E-mail : mizutani@scl.kyoto-u.ac.jp URL : http://biofun.kuicr.kyoto-u.ac.jp/index-j.html 13)

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