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1 Agilent DNA RNA
2 Lab-on-a-Chip Agilent Agilent 2100 Lab-on-a-Chip Agilent 2100 RNA DNA SDS-PAGE Agilent 2100 Agilent 2100 Agilent 2100 : (1 4 µl) CFR Part 11 Agilent 2100 Agilent Agilent 2100 ( ) Agilent 2100 ( PC ) 2
3 1 Agilent 2100 / 2100 Lab-on-a-Chip DNA RNA Lab-on-a-Chip 2100 SDS-PAGE µl 2. / RNA QC RIN Agilent ,000 qpcr RNA PCR/multiplex PCR RT-PCR DNA ( ) ( )
4 RNA RNA total RNA? mrna Cy5 RNA? RNA total RNA mrna RNA RNA Integrity Number (RIN) RNA 1 µl total RNA 50pg RNA RNA RIN total RNA mrna RNase Free RNase Free RNase Free EtBr) µl 10 RIN = Intact total RNA [ ] RIN = 5,8 Partially degraded RNA [ ] RNA RNase Agilent 2100 () total RNA RNA Integrity Number (RIN) RNA 4
5 DNA DNA DNA DNA multiplex PCR PCR DNA (Laser Induced Fluorescence) 0.1 ng DNA / (2 ) 3 ( bp bp bp ) PCR : PCR multiplex PCR RT-PCR PCR (EtBr) µl multiplex PCR PCR PCR RNA 1000 : 13 primer ( bp 13 ) multiplex PCR QIAGEN ( ) 5
6 Protein Agilent 2100 SDS-PAGE Agilent 2100 ( ) IQ OQ/PV 1 4 µl Protein 80 Protein 230 QA/QC C 12 Agilent
7 * * 2100 Agilent Lab-on-a-Chip Annexin V Caspase-3 / GFP 10 µl ( 20,000 2,500 ) 6 Agilent 2100 DNA RNA (GFP) Cy5 sirna Annexin V -3 DNA (DNA ) Annexin V (PS: ) PS Annexin V Cy5 Annexin V 7
8 Agilent 2100 RNA DNA Agilent 2100 total RNA RNA Integrity Number (RIN) (IQ OQ) 21 CFR Part 11 ( ) 1 48 (Web ) RNA DNA XML 48 ( 48 ) 8
9 Agilent 2100 Agilent 2100 IQ OQ/PV * ( ) (GLP) (GMP) Agilent 2100 DQ IQ OQ/PV CFR Part (DQ) DQ ISO/IEC Guide 22 CEN/ CENELEC EN (IQ) IQ Agilent (OQ) OQ (PQ) PQ PQ 2100 *2001 "B" "C" 1 Dr. Ludwig Huber 9
10 Agilent Agilent 2100 Agilent 2100 (PC ) 1 Agilent PC 1 2 : LabChip Agilent 2100 * (IQ) (OQ) Agilent 2100 IQ OQ ( ) *2001 "B" "C" Agilent 2100 DNA RNA 10
11 : RNA DNA Agilent RNA 6000 Agilent RNA 6000 Agilent RNA 6000 Agilent RNA 6000 Agilent total RNA mrna Assay total RNA Assay mrna Assay Small RNA Assay ng/µl ng/µl pg/µl ng/µl ng/µl 50 5,000 pg/µl 250 5,000 pg/µl ( ) (S/N > 3) ( ) (S/N > 3) 20% CV 20% CV 30% CV 30% CV 25%CV () * 100 mm Tris 100 mm Tris 50 mm Tris 50 mm Tris 125 mm NaCl 125 mm NaCl 50 mm NaCl 50 mm NaCl 15 mm MgCl2 15 mm MgCl2 10% CV 10% CV 20% CV 20% CV 25% CV / 12 / 11 / 11 / 11 / 1 µl 1 µl 1 µl 1 µl 1 µl () * nt 50 pg/µl(3<sn) 40 nt ) RNA 100 ng/µl Agilent DNA 1000 Assay Agilent DNA 7500 Assay Agilent DNA Assay 25 1,000 bp 100 7,500 bp ,000 bp ± 5 bp bp ± 5% 100 1,000 bp ± 5% 100 1,000 bp ± 5% bp ± 15% 1,000 7,500 bp ± 15% 1,000 12,000 bp ± 10% 500 1,000 bp ** ± 10%* ± 10% ± 15% ** 5% CV 5% CV 5% CV ** 20% CV 20% CV 25% CV ** bp: 15% CV 500 1,000 bp: 5% CV 100 1,000 bp: 10% CV 1,000 7,500 bp: 5% CV 100 1,000 bp: 15% CV 1,000 12,000 bp: 10% CV ng/µl ng/µl ng/µl KCL NaCl 250 mm MgCl2 15 mm KCL NaCl 250 mm MgCl2 15 mm KCL NaCl 250 mm MgCl2 15 mm / 12 / 12 / 1 µl 1 µl 1 µl 3 () 3 () 3 () * 70 bp ** Agilent Protein 80 Assay Agilent Protein 230 Assay 5 80 kda kda 10% 10% * 10% CV (CA BLG) 10% CV (BSA CA ) * 3% CV (CA BLG) 3% CV (BSA CA ) (S/N > 3) 6 ng/µl CA (15 ng/µl BSA) PBS 10 ng/µl (CA ) 6 ng/µl CA (15 ng/µl BSA) PBS 30 ng/l (BSA) 0.5 M NaCl (30 ng/µl BSA 0.5 M NaCl ) 0.5 M NaCl 60 2,000 ng/µl CA PBS 15 2,000 ng/µl CA 30 2,000 ng/µl BSA PBS 6 4,000 ng/µl CA BLG 6 5,000 ng/µl CA 15 5,000 ng/µl BSA PBS 20% CV (CA BLG) 20% CV (BSA CA ) / 10 / 4 µl 4 µl 3 () 3 () CA = BSA = BLG = * Agilent Assay 30 6/ 10 µl 4 C 3 11
12 Agilent kg (22 lbs) ( x x ) mm ( ) VAC Hz 60 VA 5 40 C IEC EN CSA UL 2 1 : () () : Copyright 2007 Agilent Technologies February 1, JAJP
3 1 2
Agilent 4200 TapeStation Agilent 4200 TapeStation 3 1 2 2 3 ScreenTape 3 Agilent 4200 TapeStation QC Agilent 4200 TapeStation Ready-to-use ScreenTape 8 PCR 96-well plate 1 96 1 qrt-pcr DNA RNA DNA / RNA
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確実に実験を成功させるために Agilent 2100 バイオアナライザ電気泳動システム AGILENT 2100 バイオアナライザ電気泳動システム 確実に実験を成功させるために サンプル品質の信頼性 実験を成功させるためには サンプルの品質が最も重要です 1999 年に販売開始した Agilent 2100 バイオアナライザ電気泳動システムはサンプル分析に Lab-on-a-Chip テクノロジーを応用した世界初のマイクロチップ型電気泳動装置です
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Agilent 2100 バイオアナライザ 1 台ですべてを可能に DNA RNA タンパク質 細胞分析の可能性を広げるプラットフォーム Agilent 2100 バイオアナライザ Lab-on-a-Chip テクノロジー 分析機器を Lab-on-a-Chip テクノロジーで小型化することにより さまざまな利点が得られます たとえば データの精度や再現性の向上 分析時間の短縮 サンプル消費量の低減
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