MicroSeq ID Analysis Software Version 1.0 Getting Started Guide
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1 MicroSeq ID Analysis Software Version 1.0 Getting Started Guide
2 Copyright 2003, Applied Biosystems. All rights reserved. For Research Use Only. Not for use in diagnostic procedures. Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Applied Biosystems be liable for incidental, special, multiple, or consequential damages in connection with or arising from the use of this document. NOTICE TO PURCHASER: DISCLAIMER OF LICENSE Purchase of this software product alone does not imply any license under any process, instrument or other apparatus, system, composition, reagent or kit rights under patent claims owned or otherwise controlled by Applera Corporation, either expressly or by estoppel. NOTICE TO PURCHASER: PLEASE REFER TO THE MICROSEQ IDENTIFICATION KIT PROTOCOL FOR LIMITED LABEL LICENSE OR DISCLAIMER INFORMATION TRADEMARKS: ABI PRISM, Applied Biosystems, BigDye, and MicroSeq are registered trademarks of Applera Corporation or its subsidiaries in the U.S. and/or certain other countries. AB (Design), Applera, and POP-6 are trademarks of Applera Corporation or its subsidiaries in the U.S. and/or certain other countries. All other trademarks are the sole property of their respective owners. Part Number Rev. A 08/2003
3 vii vii vii vii vii viii viii viii viii ix 1 : MicroSeq ID Analysis Options MicroSeqID Manager Project MicroSeq ID Analysis 1.0 Getting Started Guide iii
4 2 : / Authentication Audit / : Master Analysis Protocol MicroSeqID Manager Master Analysis Protocol Clear Range iv MicroSeq ID Analysis 1.0 Getting Started Guide
5 4 : Display Settings : Analysis QC Analysis QC Specimen Analysis Sample Analysis : Library Search Library Search Hit List Concise Alignment Phylogenetic Tree A Software Warranty Information Computer Configuration A-2 Limited Product Warranty A-2 Limited Warranty A-2 Warranty Period Effective Date A-2 Warranty Claims A-2 Warranty Exceptions A-3 Warranty Limitations A-3 MicroSeq ID Analysis 1.0 Getting Started Guide v
6 vi MicroSeq ID Analysis 1.0 Getting Started Guide
7 Applied Biosystems MicroSeq ID Analysis v1.0 Getting Started Guide MicroSeq ID Analysis MicroSeq ID Analysis CD-ROM MicroSeq ID Analysis Microsoft Winows MicroSeq ID Analysis 0 [Enter] (>) File > Open > Spot Set View Filter > View All Runs MicroSeq ID Analysis 1.0 Getting Started Guide vii
8 Applied Biosystems 2 Control Console Oracle ID Sample Entry MicroSeq ID Analysis v1.0 - MicroSeq ID Analysis MicroSeq ID Analysis [F1] Help > Contents & Index MicroSeq ID Analysis v1.0 Quick Reference Card - MicroSeq ID Analysis Quick Reference Card PDF CD-ROM ix Applied Biosystems jptechsupport@appliedbiosystems.com viii MicroSeq ID Analysis 1.0 Getting Started Guide
9 PDF Applied Biosystems MicroSeq ID Analysis 1.0 Getting Started Guide ix
10 x MicroSeq ID Analysis 1.0 Getting Started Guide
11 1 : MicroSeq ID Analysis MicroSeq ID Analysis 1.0 Getting Started Guide 1-1
12 1 : Applied Biosystems MicroSeq ID Analysis v1.0 Getting Started Guide Administrator MicroSeq ID Analysis MicroSeq ID Analysis CD-ROM Getting Started Guide MicroSeq ID Analysis MicroSeq ID Analysis v1.0 MicroSeq ABI PRISM MicroSeq ID Analysis MicroSeq ID Analysis 1.0 Getting Started Guide
13 3 Audit Analysis Protocol MicroSeq ID Analysis 1.0 Getting Started Guide 1-3
14 1 Getting Started Guide CD-ROM 1-5 : 2 : Analysis Protocol 3 : MicroSeq ID Analysis 1.0 Getting Started Guide
15 Getting Started Guide MicroSeq ID Analysis CD-ROM \AppliedBiosystems\MicroSeqID\Tutorial Data\Bacterial500Samples Bacterial500CustomLibrary 500KitCustomLibrary.fsta 500EColi MicroSeq041703_C03_H_5F_05.ab1 MicroSeq041703_D03_H_535R_07.ab1 POP-6 50 cm ABI PRISM 3100 SR BigDye Terinator v1.1 MicroSeq ID Analysis 1.0 Getting Started Guide 1-5
16 1 Getting Started Guide MicroSeq ID Analysis 3 MicroSeqID Manager Options Project MicroSeq ID Analysis : Options MicroSeqID Manager Project Project Report Manager Sample Manager Specimen Analysis QC Analysis Protocol Master Analysis Protocol Sample Library Search Audit Trail Options Options Tools > Options MicroSeqID Manager MicroSeqID Manager Tools > MicroSeqID Manager Options Authentication MicroSeqID Manager Master Analysis Protocol Project Project File > Open Project Project Project Specimen Sample Sample Manager Report Manager 1-6 MicroSeq ID Analysis 1.0 Getting Started Guide
17 MicroSeq ID Analysis New Project [Ctrl] + [N] Save Project [Ctrl] + [S] Import Samples To Project [Ctrl] + [M] Find Again [F3] Print [Ctrl] + [P] Delete MicroSeqID Manager MicroSeqID Manager Undo Base Change [Ctrl] + [Z] Open Project [Ctrl] + [O] Find [Ctrl] + [F] /IUB New Specimen MicroSeq ID Analysis 1.0 Getting Started Guide 1-7
18 1 Analyze Samples [Ctrl] + [R] Analysis Settings [Ctrl] + [B] Analysis Protocol Analysis Settings Sample Manager Sample Manager Report Manager [Ctrl] + [1] Report Manager MyScience Applied Biosystems MyScience Web Display Settings [Ctrl] + [Y] Display Settings Show/Hide Electropherogram Specimen Assembly Show/Hide sample QV [Ctrl] + [K] Full View of Electropherogram [Ctrl] + []] Zoom Out [Ctrl] + [-] Inverse View Inverse View Aligned EP Show/Hide All Electropherograms Zoom In [Ctrl] + [=] Show/Hide consensus QV View Original Sequence Sample Electropherogram View Column Selector 1-8 MicroSeq ID Analysis 1.0 Getting Started Guide
19 % ( / 100 = = Analysis Protocol Clear Range Clear Range Analysis Protocol MSID_A03_C_781F.ab1 _ _ A03 Quality Values QV Quality Values QV MicroSeq MicroSeq 50016S rdna Bacterial Identification Kit MicroSeq Ful Gene 16S rdna Bacterial Identification Kit MicroSeq D2 DNA Fungal Identification Kit MicroSeq ID Analysis Clear Range Basecaller MicroSeq ID Analysis QV MicroSeq ID Analysis 1.0 Getting Started Guide 1-9
20 1 MicroSeq ID Analysis DNA \ / : *? < > Ç C Getting Started Guide MicroSeq ID Analysis MicroSeq ID Analysis CD-ROM MicroSeq ID Analysis [F1] Help > Contents & Index GPS Explorer MicroSeq ID Analysis 1-10 MicroSeq ID Analysis 1.0 Getting Started Guide
21 : 2 MicroSeq ID Analysis Authentication Audit Authentication Audit MicroSeq ID Analysis 1.0 Getting Started Guide 2-1
22 2 : MicroSeq ID Analysis Getting Started Guide : MicroSeq ID Analysis 1.0 Getting Started Guide
23 A Software Warranty Information A MicroSeq ID Analysis MicroSeq ID Analysis Applied Biosystems Applied Biosystems MicroSeq ID Analysis : MicroSeq ID Analysis 1.0 Getting Started Guide 2-3
24 2 : MicroSeq ID Analysis CPU CD-ROM 733 MHz Intel Pentium III IV Intel Xeon Microsoft Winows 2000 OS Service Pack 2 Service Pack 3 Microsoft Winows XP OS Service Pack 1 RAM 256 MB 512 MB 2 GB MicroSeq ID Analysis MicroSeq ID Analysis HP cxi Epson MicroSeq ID Analysis 1.0 Getting Started Guide
25 MicroSeq ID Analysis :\Applied Biosystems\MicroSeqID Applied Biosystems MicroSeq ID Analysis C <10% <200 MB 10% MicroSeq ID Analysis C D 2-2 D C D Applied Biosystems 3730/3730xl DNA Analyzer Data Collection E MicroSeq ID Analysis 1.0 Getting Started Guide 2-5
26 2 : MicroSeq ID Analysis 2 GB MicroSeq ID Analysis 1. MicroSeq ID nalysis v1.0 CD CD-ROM 2. CD-ROM setup.exe InstallShield Wizard Complete Finish 2-6 MicroSeq ID Analysis 1.0 Getting Started Guide
27 MicroSeq ID Analysis MicroSeq ID Analysis Admin 1. MicroSeq ID Analysis 2. MicroSeq ID Analysis Registration User Name Password OK Login 5. MicroSeq ID Analysis 1.0 Getting Started Guide 2-7
28 2 : 6. OK MicroSeq ID Analysis 2-8 MicroSeq ID Analysis 1.0 Getting Started Guide
29 C:\ 1. MicroSeq ID Analysis Tools > Options Options 2. General 3. Display Reports after Analysis 4. Export Reports after Analysis Format MicroSeq ID Analysis 1.0 Getting Started Guide 2-9
30 2 : 5. Default Path for Import/Export a. BrowseImport/Export Default Directory Location b. c. Set Directory 6. OK Options 2-10 MicroSeq ID Analysis 1.0 Getting Started Guide
31 MicroSeq ID Analysis Applied Biosystems MicroSeq ID Analysis Users 1. MicroSeq ID Analysis Tools > Options Options 2. Users 3. NewUser Management 4. User Name First Name Last Name Password User Name Microsoft Windows User Group Administrator Scientist Analyst MicroSeq ID Analysis 1.0 Getting Started Guide 2-11
32 2 : 6. OK Users 7. OKOptions MicroSeq ID Analysis 1. MicroSeq ID Analysis Tools > Options Options 2. Users 3. OpenUser Management 4. Inactive 3 Unlock 5. OK Users 6. OKOptions / / 2-10 MicroSeq ID Analysis / MicroSeq ID Analysis [F1] Help > Contents & IndexIndex user account export import 2-12 MicroSeq ID Analysis 1.0 Getting Started Guide
33 Authentication Audit Authentication Audit MicroSeq ID Analysis 21 CFR Part MicroSeq ID Analysis Tools > Options Options 2. Authentication & Audit 3. Authentication Settings Lockout user after invalid login attempts Within minutes Maintain lockout for minutes Automatic timeout after minutes MicroSeq ID Analysis 1.0 Getting Started Guide 2-13
34 2 : Change password every days Authentication & Audit Audit Trail Audit Trail On - Silent Audit Trail On- 2. New Audit Reason Editor 3. Reason Getting Started Guide Base Change 4. Description 5. OK Options Audit Reason 6. OK / / 2-10 Authentication & Audit MicroSeq ID Analysis Authentication & Audit / MicroSeq ID Analysis [F1] Help > Contents & Index Index Authentication and Audit settings export import 2-14 MicroSeq ID Analysis 1.0 Getting Started Guide
35 : 3 MicroSeq ID Analysis Master Analysis Protocol Master Analysis Protocol MicroSeq ID Analysis 1.0 Getting Started Guide 3-1
36 3 : MicroSeq ID Analysis Analysis Protocol Getting Started Guide 2 Getting Started Guide : 3-3 Analysis Protocol MicroSeq ID Analysis Log In 2. OK 3-2 MicroSeq ID Analysis 1.0 Getting Started Guide
37 MicroSeq ID Analysis Applied Biosystems 1. MicroSeq ID Analysis MicroSeqID Manager Tools > MicroSeqID Manager MicroSeqID Manager 2. Libraries 3. New Library Editor General MicroSeq ID Analysis 1.0 Getting Started Guide 3-3
38 3 : 4. General a. Name AB_Bacterial500CustomLibrary b. Kit Bacterial500Kit c. Comments Custom library for the Getting Started Guide (Bacterial 500 Kit) 5. EntriesImportImport Library Sequence Entries 3-4 MicroSeq ID Analysis 1.0 Getting Started Guide
![6. a. Files of typefasta format (*.fsta) b.](/docs-images/88/116844367/images/39-1.jpg "\AppliedBiosystems\MicroSeqID\Tutorial Data\Bacterial500Samples\")
39 6. a. Files of typefasta format (*.fsta) b. \AppliedBiosystems\MicroSeqID\Tutorial Data\Bacterial500Samples\ Bacterial500CustomLibrary c. 500KitCustomLibrary.fsta d. Import 7. OK 8. AB_Bacterial500CustomLibrary Library Editor Entries Library Sequence Entry MicroSeq ID Analysis 1.0 Getting Started Guide 3-5
40 3 : 9. OK Library Editor MicroSeqID Manager Libraries 3-6 MicroSeq ID Analysis 1.0 Getting Started Guide
41 Master Analysis Protocol Master Analysis Protocol Master Analysis Protocol MicroSeq ID Analysis Clear Range Master Analysis Protocol Basecalling Mixed Bases Clear Range Filter Getting Started Guide GSG_AnalysisProtocol GSG_AnalysisProtocol 4 Master Analysis Protocol MicroSeqID Manager Master Analysis Protocol Master Analysis Protocol MicroSeqID Manager Analysis Protocol Editor Master Analysis Protocol Master Analysis Protocol 1. MicroSeq ID Analysis MicroSeqID Manager Tools > MicroSeqID Manager MicroSeqID Manager 2. Analysis Protocols 3. New Analysis Protocol Editor General 4. Name GSG_AnalysisProtocol MicroSeq ID Analysis 1.0 Getting Started Guide 3-7
42 3 : 5. Comments New master analysis protocol for the Getting Started Guide 6. Analysis Protocol Editor OK Basecalling 1. Analysis Protocol Editor Basecalling 2. Basecalling a. Basecaller Basecaller-3100POP6SR.bcp b. DyeSet/Primer DT3100POP6{BD}v2.mob 3. Ending BaseAt PCR Stop 3-8 MicroSeq ID Analysis 1.0 Getting Started Guide
43 Master Analysis Protocol 4. Basecalling Basecalling Processed Data Quality Threshold KB Basecaller 5. Analysis Protocol Editor OK 2 1. Analysis Protocol Editor Mixed Bases 2. IUB Use Mixed Base Identification % 4. Mixed Bases MicroSeq ID Analysis 1.0 Getting Started Guide 3-9
44 3 : Clear Range Analysis Protocol Editor OK Clear Range Clear Range Spacing Clear Range 5 3 Clear Range 1. Analysis Protocol Editor Clear Range 2. Use quality values 3. Clear Range fewer than 4 bases out of 20 with QVs less than QV MicroSeq ID Analysis Quality Values QV QV QV 20 = 1% 4. Clear Range Analysis Protocol Clear Range Clear Range 5. Analysis Protocol Editor OK 3-10 MicroSeq ID Analysis 1.0 Getting Started Guide
45 Master Analysis Protocol Analysis Settings 1. Analysis Protocol Editor Filter 2. Maximum Mixed Bases (%) = 20.0 Maximum Ns (%) = 10.0 Minimum Clear Length (bp) = 50 Minimum Sample Score = 20 Clear Range Clear Range Analysis QC Clear Range N Clear Range N Analysis QC Clear Range Clear Range Analysis QC Clear Range QV 1 50 Clear Range Analysis QC 3. Filter 4. OK Analysis Protocol Analysis Protocol Editor GSG_AnalysisProtocol MicroSeqID Manager Analysis Protocols MicroSeq ID Analysis 1.0 Getting Started Guide 3-11
46 3 : 3-12 MicroSeq ID Analysis 1.0 Getting Started Guide
47 : 4 MicroSeq ID Analysis Display Settings : Analysis QC : Library Search MicroSeq ID Analysis 1.0 Getting Started Guide 4-1
48 4 : MicroSeq ID Analysis Getting Started Guide 2 Getting Started Guide 3 Master Analysis Protocol GSG_AnalysisProtocol 3-7 Getting Started Guide : MicroSeq ID Analysis Log In 2. OK 4-2 MicroSeq ID Analysis 1.0 Getting Started Guide
49 Display Settings Display Settings Display Settings Display Settings Display Settings 1. MicroSeq ID Analysis Analysis > Display Settings Display Settings 2. General 3. Display Settings For User 4. Comments My display settings 5. Bases MicroSeq ID Analysis 1.0 Getting Started Guide 4-3
50 4 : 6. Electropherogram 7. Views General Sample Specimen 8. Save Display Settings Display Settings Display Settings Display Settings 4-4 MicroSeq ID Analysis 1.0 Getting Started Guide
51 1. MicroSeq ID Analysis File > New Project New Project Select Project Settings 2. Project Name 500KitProject 3. Matches to Display 5 Matches to Display Library Search Results Project KitBacterial500Kit Kit MicroSeq Bacterial500Kit MicroSeq S rdna Bacterial Identification Kit 5. Analysis ProtocolGSG_AnalysisProtocol GSG_AnalysisProtocol 3 Analysis Protocol MicroSeq ID Analysis 1.0 Getting Started Guide 4-5
52 4 : 6. Libraries AvailableBacterial500Lib 4 Applied Biosystems Applied Biosystems Applied Biosystems 7. Select Project Settings MicroSeq ID Analysis 1.0 Getting Started Guide
53 MicroSeq ID Analysis.ab1 Getting Started Guide 2 MicroSeq041703_C03_H_5F_05.ab1 MicroSeq041703_D03_H_535R_07.ab1 New Specimen Getting Started Guide 4-8 MicroSeq ID Analysis [F1] Help > Contents & IndexIndex new project New Project - Create Specimens Dialog Box MicroSeq ID Analysis 1.0 Getting Started Guide 4-7
54 4 : 1. New Project Select Project Settings Next Create Specimens 2. New Specimen Specimen1 Samples to Add Specimen1 Project Navigator Files 500Ecoli \AppliedBiosystems\MicroSeqID\Tutorial Data\Bacterial500Samples\ 500EColi Getting Started Guide 4-8 MicroSeq ID Analysis 1.0 Getting Started Guide
55 4. Add Sample 500Ecoli Samples To Add Specimen New Project Create Specimens Next Finish a. Back Create Specimens Select Project Settings b. c. Next Finish MicroSeq ID Analysis 1.0 Getting Started Guide 4-9
56 4 : Finish and Analyze Finish Getting Started Guide MicroSeq ID Analysis [F1] Help > Contents & IndexIndex analyzing data analyzing 4-10 MicroSeq ID Analysis 1.0 Getting Started Guide
![Project Navigator a. Specimen1 b. Uknown_1 c. [Enter] [Enter] 3.](/docs-images/88/116844367/images/57-1.jpg "Project Navigator a. % 500KitProject MicroSeq ID Analysis 1.")
57 1. New Project Finish Finish and Analyze Analysis Status Analysis QC Tools > Options > General Display Reports after Analysis Project 2. Project Navigator a. Specimen1 b. Uknown_1 c. [Enter] [Enter] 3. Project Navigator a. % 500KitProject MicroSeq ID Analysis 1.0 Getting Started Guide 4-11
58 4 : b. Layout Assembly Unknown_ : Analysis QC 4-12 MicroSeq ID Analysis 1.0 Getting Started Guide
59 : Analysis QC : Analysis QC Analysis QC Analysis QC Analysis QC 1. Project Project Navigator 500KitProject 2. Analysis > Report Manager 500KitProject Report Manager 3. Reports Analysis QC Report Reports Report Settings Wrap Text Analysis QC Project Window > Tile 4. Specimen Analysis 4-14 Specimen Analysis 5. Specimen Analysis 4-16 Sample Analysis : Library Search MicroSeq ID Analysis 1.0 Getting Started Guide 4-13
60 4 : Specimen Analysis Specimen Analysis Specimen - Specimen # Samples - Basecalling Filter Assembly - Analysis Quality Specimen Score - Quality Values QV 4-15 Top Match - % % Match - Top Match 4-15 % Analysis Quality Analysis Quality Basecalling Filter Assembly 4-1 Analysis Quality : : : Analysis QC Sample Analysis Partial Output No Output Sample Analysis MicroSeq ID Analysis 1.0 Getting Started Guide
61 : Analysis QC Specimen Score QV QV QV QV QV = 10log 10 (Pe) Pe QV QV QV QV 1 50 [F1] Help > Contents & IndexIndex quality value 4-2 QV Pe QV Pe 10 a 10% % 20 a 1% 40 a 0.01% % % 30 a 0.1% 50 a 0.001% a. Low Confidence Medium Confidence High Confidence % % % Match Top Match % ( / 100 = = MicroSeq ID Analysis 1.0 Getting Started Guide 4-15
62 4 : Sample Analysis Specimen Analysis Partial Output No Output Sample Analysis Sample Analysis Sample - Step - Description Specimen1 Filter Partial Output Sample Analysis Sample Analysis MicroSeq041703_C03_H_5F_ Specimen1 Specimen 4-2 Sample 4-2 Sample 4-16 MicroSeq ID Analysis 1.0 Getting Started Guide
63 : Library Search : Library Search Library Search Neighbor Joining Tree Library Search Library Search 1. Project Project Navigator 500KitProject 2. Analysis > Report Manager 500KitProject Report Manager 3. Reports Library Search Report Reports Report Settings Wrap Text Library Search Project Window > Tile 4. Hit List 4-18 Hit List 5. Concise Alignment 4-18 Concise Alignment MicroSeq ID Analysis 1.0 Getting Started Guide 4-17
64 4 : 6. Phylogenetic Tree 4-19 Phylogenetic Tree Hit List Concise Alignment Hit List Specimen - Library - Sequence Name - % Match - Top Match 4-15 % # of Bases Searched - Clear Range Total Mismatches - Concise Alignment% <100 MicroSeq ID Analysis 0 Concise Alignment Unknown_3 Brevibacterium casei49? 50 C - C T G G? 4-18 MicroSeq ID Analysis 1.0 Getting Started Guide
65 : Library Search Phylogenetic Tree Phylogenetic Tree Hit List Specimen - N.Join % - - MicroSeq ID Analysis Neighbor Joining Tree 16S rrna Unknown_1 MicroSeq ID Analysis 1.0 Getting Started Guide 4-19
66 4 : 4-14 Analysis Quality Getting Started Guide Clear Range MicroSeq ID Analysis 1. File > Open Project Project 2. Project Navigator 3. Analysis > Report Manager 4. Reports Analysis QC Report Specimen 5. Specimen Analysis Specimen 4-20 MicroSeq ID Analysis 1.0 Getting Started Guide
67 6. Specimen Assembly Assembly QV QV 7. QV QV Project Show/Hide consensus QV Show/Hide sample QV 8. Project Show/Hide All Electropherograms 9. [Delete] OK Audit Reason Editor OK MicroSeq ID Analysis 1.0 Getting Started Guide 4-21
68 4 : 1. File > Open Project Project 2. Project Navigator 3. Analysis > Sample Manager Sample Manager Analysis Protocol Edit Analysis Protocol Apply Analysis Protocol 4. Sample Name BaseCaller DyeSet/Primer Spacing Peak 1 Start Stop MicroSeq ID Analysis [F1] Help > Contents & IndexIndex sample manager parameters 5. OK Audit Reason Editor Reason OK Project Analyze samples 2. Analysis QC Library Search File > Close Project 5. Yes Project 4-22 MicroSeq ID Analysis 1.0 Getting Started Guide
69 Analysis QC Library Search Audit Trail Getting Started Guide 500KitProject Analysis QC 500KitProject Analysis QC 1. File > Open Project 500KitProjectProject 2. Project Navigator 3. Analysis > Report Manager 4. Reports Analysis QC Report 5. Report SettingsReport Settings MicroSeq ID Analysis 1.0 Getting Started Guide 4-23
70 4 : 6. Horizontal Scrolling - Report Manager Font Size Wrap Text OK Analysis QC 500KitProject Analysis QC 1. Analysis QC File > Export > Report Export Report KitProject_Analysis_QC_Report 4. PDF HTML XML TXT 5. Export 4-24 MicroSeq ID Analysis 1.0 Getting Started Guide
71 500KitProject Analysis QC 1. Analysis QC File > Print Print Report Header/Footer 4. Print preview 5. Print 6. OK MicroSeq ID Analysis 1.0 Getting Started Guide 4-25
72 4 : 4-26 MicroSeq ID Analysis 1.0 Getting Started Guide
73 Software Warranty Information A Computer Configuration A-2 Limited Product Warranty A-2 MicroSeq ID Analysis Software Version 1.0 Getting Started Guide A-1
74 A Software Warranty Information Computer Configuration Applied Biosystems supplies or recommends certain configurations of computer hardware, software, and peripherals for use with its instrumentation. Applied Biosystems reserves the right to decline support for or impose extra charges for supporting nonstandard computer configurations or components that have not been supplied or recommended by Applied Biosystems. Applied Biosystems also reserves the right to require that computer hardware and software be restored to the standard configuration prior to providing service or technical support. For systems that have built-in computers or processing units, installing unauthorized hardware or software may void the Warranty or Service Plan. Limited Product Warranty Limited Warranty Warranty Period Effective Date Warranty Claims Applied Biosystems warrants that for a period of ninety (90) days from the date the warranty period begins, its MicroSeq ID Analysis software will perform substantially in accordance with the functions and features described in its accompanying documentation when properly installed on the instrument system for which it is designated, and that for a period of ninety (90) days from the date the warranty period begins, the tapes, diskettes, or other media bearing the software product will be free of defects in materials and workmanship under normal use. If buyer believes that it has discovered a failure of the software to satisfy the foregoing warranty, and if buyer notifies Applied Biosystems of such failure in writing during the ninety (90) day warranty period, and if Applied Biosystems is able to reliably reproduce such failure, then Applied Biosystems, at its sole option, will either (i) provide any software corrections or bugfixes of the identified failure, if and when they become commercially available, to buyer free of charge, or (ii) notify buyer that Applied Biosystems will accept a return of the software from the buyer and, upon such return and removal of the software from buyer's systems, terminate the license to use the software and refund the buyer's purchase price for the software. If there is a defect in the media covered by the above warranty and the media is returned to Applied Biosystems within the ninety (90) day warranty period, Applied Biosystems will replace the defective media. Applied Biosystems does not warrant that the software will meet buyer's requirements or conform exactly to its documentation, or that operation of the software will be uninterrupted or error free. Any applicable warranty period under these sections begins on the earlier of the date of installation or ninety (90) days from the date of shipment for software installed by Applied Biosystems personnel. For all software installed by the buyer or anyone other than Applied Biosystems, the applicable warranty period begins the date the software is delivered to the buyer. Warranty claims must be made within the applicable warranty period. A-2 MicroSeq ID Analysis Software Version 1.0 Getting Started Guide
75 Limited Product Warranty Warranty Exceptions Warranty Limitations The above warranties do not apply to defects resulting from misuse, neglect, or accident, including without limitation: operation outside of the environmental or use specifications, or not in conformance with the instructions for the instrument system, software, or accessories; improper or inadequate maintenance by the user; installation of software or interfacing, or use in combination with software or products, not supplied or authorized by Applied Biosystems; and modification or repair of the product not authorized by Applied Biosystems. THE FOREGOING PROVISIONS SET FORTH APPLIED BIOSYSTEMS' SOLE AND EXCLUSIVE REPRESENTATIONS, WARRANTIES, AND OBLIGATIONS WITH RESPECT TO ITS PRODUCTS, AND APPLIED BIOSYSTEMS MAKES NO OTHER WARRANTY OF ANY KIND WHATSOEVER, EXPRESSED OR IMPLIED, INCLUDING WITHOUT LIMITATION, WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE, WHETHER ARISING FROM A STATUTE OR OTHERWISE IN LAW OR FROM A COURSE OF DEALING OR USAGE OF TRADE, ALL OF WHICH ARE EXPRESSLY DISCLAIMED. THE REMEDIES PROVIDED HEREIN ARE THE BUYER'S SOLE AND EXCLUSIVE REMEDIES. WITHOUT LIMITING THE GENERALITY OF THE FOREGOING, IN NO EVENT SHALL APPLIED BIOSYSTEMS BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE (INCLUDING WITHOUT LIMITATION ANY TRADE PRACTICE, UNFAIR COMPETITION, OR OTHER STATUTE OF SIMILAR IMPORT) OR ON ANY OTHER BASIS, FOR DIRECT, INDIRECT, PUNITIVE, INCIDENTAL, MULTIPLE, CONSEQUENTIAL, OR SPECIAL DAMAGES SUSTAINED BY THE BUYER OR ANY OTHER PERSON OR ENTITY, WHETHER OR NOT FORESEEABLE AND WHETHER OR NOT APPLIED BIOSYSTEMS IS ADVISED OF THE POSSIBILITY OF SUCH DAMAGES, INCLUDING WITHOUT LIMITATION, DAMAGES ARISING FROM OR RELATED TO LOSS OF USE, LOSS OF DATA, FAILURE OR INTERRUPTION IN THE OPERATION OF ANY EQUIPMENT OR SOFTWARE, DELAY IN REPAIR OR REPLACEMENT, OR FOR LOSS OF REVENUE OR PROFITS, LOSS OF GOOD WILL, LOSS OF BUSINESS, OR OTHER FINANCIAL LOSS OR PERSONAL INJURY OR PROPERTY DAMAGE. NO AGENT, EMPLOYEE, OR REPRESENTATIVE OF APPLIED BIOSYSTEMS HAS ANY AUTHORITY TO MODIFY THE TERMS OF THIS LIMITED WARRANTY STATEMENT OR TO BIND APPLIED BIOSYSTEMS TO ANY AFFIRMATION, REPRESENTATION, OR WARRANTY CONCERNING THE PRODUCT THAT IS NOT CONTAINED IN THIS LIMITED WARRANTY STATEMENT, AND ANY SUCH MODIFICATION, AFFIRMATION, REPRESENTATION, OR WARRANTY MADE BY ANY AGENT, EMPLOYEE, OR REPRESENTATIVE OF APPLIED BIOSYSTEMS WILL NOT BE BINDING ON APPLIED BIOSYSTEMS UNLESS IN A WRITING SIGNED BY AN EXECUTIVE OFFICER OF APPLIED BIOSYSTEMS. THIS WARRANTY IS LIMITED TO THE BUYER OF THE PRODUCT FROM APPLIED BIOSYSTEMS AND IS NOT TRANSFERABLE. Some countries or jurisdictions limit the scope of or preclude limitations or exclusion of warranties, of liability, such as liability for gross negligence or willful misconduct, or of remedies or damages, as or to the extent set forth above. In such countries and jurisdictions, the limitation or exclusion of warranties, liability, remedies or damages set forth above shall apply to the fullest extent permitted by law, and shall not apply to the extent prohibited by law. MicroSeq ID Analysis Software Version 1.0 Getting Started Guide A-3
76 A Software Warranty Information A-4 MicroSeq ID Analysis Software Version 1.0 Getting Started Guide
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DS-30
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準備と設定
ii iii iv v vi 1 2 3 4 vii 5 6 7 8 9 viii This product (including software) is designed under Japanese domestic specifications and does not conform to overseas standards. NEC* 1 will not be held responsible
準備と設定
ii iii iv v vi 1 2 3 vii 4 5 6 7 8 viii 9 ix This product (including software) is designed under Japanese domestic specifications and does not conform to overseas standards. NEC* 1 will not be held responsible
準備と設定
ii iii iv v vi 1 2 3 vii 4 5 6 7 8 viii 9 ix This product (including software) is designed under Japanese domestic specifications and does not conform to overseas standards. NEC* 1 will not be held responsible
2011 Shinano Kenshi Co.,Ltd. ... 2... 12... 12... 18... 19... 21... 21... 23 1.2.1....23 1.2.2....24 1.2.3....26 1.2.4....27... 29... 30... 30... 31... 38... 39... 40 2 ...41...42...43...43 3.1.1... 44
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PX-504A
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PX-434A/PX-404A
NPD4534-00 ...6... 6...10 Mac OS X...11 Mac OS X v10.5.x v10.6.x...11 Mac OS X v10.4.11...15...18...19...19...21...22!ex...22 /...23 P.I.F. PRINT Image Framer...23...24...26...27...27...28...28...31 Web...31...31...35...35...35...37...37...37...39...39...40...43...48
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GT-F740/GT-S640
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EP-704A
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Zinstall WinWin 日本語ユーザーズガイド
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ユーザーズマニュアル
1 2 3 4 This product (including software) is designed under Japanese domestic specifications and does not conform to overseas standards. NEC *1 will not be held responsible for any consequences resulting
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Unify NXJ Release 12 2002-2008 Unify Corporation All rights reserved. Sacramento California, USA No part of this tutorial may be reproduced, transmitted, transcribed, stored in a retrieval system, or translated
2.4 DSOF 4 1 2 3 4 1 2 3 4 5 6 7 8 9 10 11 12 1 2 SET RESET POWER PPP PPP 3 POWER DATA 4 SET RESET WAN PC1 PC2 5 POWER PPP DATA AIR 6 1 2 3 4 5 6 7 II II II 8 1 2 3 4 5 6 7 8 9 10 II
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HT7-0199-000-V.5.0 1. 2. 3. 4. 5. 6. 7. 8. 9. Copyright 2004 CANON INC. ALL RIGHTS RESERVED 1 2 3 1 1 2 3 4 1 2 1 2 3 1 2 3 1 2 3 1 2 3 4 1 2 3 4 1 2 3 4 5 AB AB Step 1 Step
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Introduction Purpose This training course demonstrates the use of the High-performance Embedded Workshop (HEW), a key tool for developing software for
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2.4 DSOF 4 RESET WAN LAN1 LAN2 LAN3 LAN4 DC-IN 12V 1 2 3 4 ON 1 2 3 4 ON 1 2 3 4 5 6 7 8 1 2 3 4 5 1 2 3 4 5 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 1 2 3 4 5 3 1 2 1 2 3 4
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1 2 3 4 5 6 7 2.4 DSOF 4 1 1 POWER LINK AIR 1 1 1 1 1 1 POWER LINK AIR 1 1 DC-IN SET RESET WAN PC1 PC2 PC3 PC4 1 POWER LINK AIR 1 POWER PC1 PC2 PC3 PC4 DC-IN DC5V LINK AIR 1 1 1
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EP-904 EP-804 EP-774A NPD4532-00 ...7... 7...11 Mac OS X...12 Mac OS X v10.5.x v10.6.x...12 Mac OS X v10.4.11...16...19...20...20...23...24...25!ex...25 /...26 P.I.F. PRINT Image Framer...26...27...31...31...32...32...32...36
操作ガイド（本体操作編）
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NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
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NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信
NEXT FUNDS NASDAQ-100 連動型上場投信 TOPIX Exchange Traded Fund Year Index Value Daily NAV Daily Return Unit NAV -Month Day Return (per Unit) Return Differential Outstanding (i) The TOPIX Index Value and the
NEXT FUNDS NASDAQ-100 連動型上場投信
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