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1 N AMY Mycobacteria Growth Indicator Tube 4,7 1,10 2OADC Enrichment 3 PANTAPANTA Antibiotic Mixture, Lyophilized B600g/ 4,7 1,10 7H PANTA 1 PANTA 365nm UV 1 1 Centers for Disease Control and PreventionCDC Public Health Mycobacteriology. A Guide for the Level Laboratory 3, 4 N L NALC NALC/NaOH NALC/NaOH 1 2 NALC/NaOH ph g 20 PBS0.6mL NALC/NaOH4% 50mL 2.9% 50mL NALC 0.5g 1
2 2NALC/NaOH PBSpH6.8 3,000g20 PBS0.6mL 2 10mL 3,000g mL 3,000g 20 5mL 1 2mL 4 3,000g mL 2 NALC/NaOH ph6.83,000g 20 PBS0.6mL NALC/NaOH4% 50mL 2.9% 50mL NALC 0.5g 2 CDC Clinical Microbiology Procedures Handbook 3, 4, NALC/NaOH mL NALC/NaOH 1 50mL 2NALC/NaOH 5mL mL ph6.8 53,000g 20 6 ph mL PANTA 2
3 2 1 PANTA 1 4L PANTA 3mL 2 7mL 1 2 /PANTAPANTA 15mL 2 4mL UV 365nm 7mL BD MGIT MGIT 3 1 4mL 1 PANTA 3mL 4mL 0.5mL 3mL PANTA 0.1mL NALC/NaOH 0.5mL 0.1mL 1% 372 UV 365nm PANTA3mL 4mL 0.5mL PANTA0.1mL NALC/NaOH0.5mL 37UV365nm 2 4mL 0.4% 5mL mL 1 7mL PANTA0.8mL NALC/NaOH 0.5mL 0.1mL 1% 2MGIT BD MGIT 3
4 PANTA15mL 7mLPANTA0.8mL 7mLNALC/NaOH0.5mL MGIT 1 4mL 2 UV CFU/mL 4mL UV 365nm UV 4mL 8 2 7mL MGIT n %581/ % 82.7% DDH PCR 4
5 70 30 DDH 2 1n %67/68 100% 97.8% 2 3 7H11 Mycobaterium tuberculosis Complex 3 n %57/57 100% 100% 4 n %50/50 100% 100% 5 n50 7H %50/50 100% 5
6 100% 2 24 ATCC CFU/mL M.africanum M.gordonae M.nonchromogenioum M.terrae M.avium complex M.haemophilum M.phlei M.triviale M.chelonae M.intracellulare M.scrofulaceum M.tuberculosis M.flavescens M.kansasii M.simiae M.vaccae M.fortuitum M.malmoense M.smegmatis M.xenopi M.gastri M.marinum M.szulgai M.abscessus Mycobacterium tuberculosis CDC mL 3 UV 365nm UV 4 Mycobacterium haemophilum 30 BBL X 1 MGIT mL 3 4 7mL 6 7 PANTA
7 PANTA 2 8 PANTA PANTA 9 7H9 ATCC 115 7H ATCC 0.5 M.tuberculosis M.kansasii M.fortuitum CFU/mL MGIT CDC 3 4 2% ph PANTA mL 7mL MGIT 960 PANTA mL 4mL mL 4mL PANTA PANTA
8 1. Cohn, M. L. R. F. Waggoner, and J. K. McClatcry The 7H11 medium for the cultivation of mycobacteria. mycobacteria. Am. Rev. Resp. Dis Youmans.G.P.1979.Cultivation of mycobacteria,the morphology and metabolism of mycobactera.p Tuberculosis. W. B. SaundersCompany,Philadelphia. 3. Kent.P.T., and G.P.Kubica, Public health mycobacteriology : A guide for the level III laboratory. USDHHS, Centers for Disease Control, Atlanta Isenberg,Henry D.1992 Clinical microbiology procedures handbook,vol.1.american Society for Microbiology. 6. Washington.D.C.Bloodborne pathogens.code of Federal Regulations.Title 29, Part Federal Register 1991, 56 : BD BD TEL FAX TEL
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