MACS機器予算申請カタログ

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1 MACS Instruments MACSQuant automacs MultiMACS gentlemacs MACSmix TM

Orange, VioGreen TM Blue 488 nm Red 635 nm Blue 488 nm B1 B2 B3 B4 R1 R2 FSC SSC 525/50 585/40 655-730 750 LP 655-730

2 MACSQuant 3 MACSQuant Analyzer 10 MACSQuant VYBMACSQuant X MACSQuant X Coming soon! 384 well plate Laser Channel Filter (nm) Dye or Parameter Violet 405 nm V1 450/50 CFP, VioBlue V2 525/50 Pacific Orange, VioGreen TM Blue 488 nm Red 635 nm Blue 488 nm B1 B2 B3 B4 R1 R2 FSC SSC 525/50 585/ LP LP 488/10 488/10 GFP, FITC, VioBright TM FITC PE PI, PerCP, PE-Cy 5.5, PerCP-Vio700 PE-Cy7, PE-Vio770 TM APC APC-Cy7, APC-Vio770 TM Size Granularity 1

MACSQuant Analyzer 10 Laser Channel Filter (nm) Dye or Parameter Violet 405 nm V1 V2 450/50 525/50 CFP, VioBlue Pacific Orange, VioGreen TM Blue 488 nm

5, PerCP-Vio700 PE-Cy7, PE-Vio770 TM Red 635 nm R1 R2 655-730 750 LP APC APC-Cy7, APC-Vio770 TM Blue 488 nm FSC SSC 488/10 488/10 Size Granularity 12.

3 MACSQuant Analyzer 10 Laser Channel Filter (nm) Dye or Parameter Violet 405 nm V1 V2 450/50 525/50 CFP, VioBlue Pacific Orange, VioGreen TM Blue 488 nm B1 B2 B3 B4 525/50 585/ LP GFP, FITC, VioBright TM FITC PE PI, PerCP, PE-Cy 5.5, PerCP-Vio700 PE-Cy7, PE-Vio770 TM Red 635 nm R1 R LP APC APC-Cy7, APC-Vio770 TM Blue 488 nm FSC SSC 488/10 488/10 Size Granularity ,E+07 Counts/mL Cells/mL 2,E+07 1,E+07 A B Dilution factor A Spiked SKB-BR3 cells in 10 8 WBCs B Original fraction Enriched fraction CD CD45 2

MACSQuant MACSQuant VYB Yellow (561nm) Laser Channel Filter (nm) Dye or Parameter Violet 405 nm Blue 488 nm Yellow 561 nm Yellow 561 nm V1 V2 B1 B2 Y1 Y2 Y3 Y4 FSC SSC 450/50 525/50 525/50 614/50

4 MACSQuant MACSQuant VYB Yellow (561nm) Laser Channel Filter (nm) Dye or Parameter Violet 405 nm Blue 488 nm Yellow 561 nm Yellow 561 nm V1 V2 B1 B2 Y1 Y2 Y3 Y4 FSC SSC 450/50 525/50 525/50 614/50 586/15 615/20 661/ LP 561/10 561/10 CFP, VioBlue Pacific Orange, GFP, YFP, VioGreen TM GFP, FITC, YFP, VioBright TM FITC PI, Lss-mKate, YFP PE mcherry, DsRed, Texas Red Pe-Cy5, APC, mkate PE-Cy7, PE-Vio770, APC-Vio770 Size Granularity A Relative cell number D MFI : 30.7 SI: 26.7 Relative cell number MFI : 32.8 SI: ¹ 10² ¹ 10² CD3-FITC CD3-FITC CFP egfp YFP B Relative cell number MFI FI: 49.3 SI: 64.8 E Relative cell number MFI: 88.8 SI: ¹ 10² ¹ 10² mcherry mkate 2 PI CD4-PE CD4-PE C Relative cell number MFI: SI: ¹ 10² F Relative cell number MFI: SI: ¹ 10² CD8-APC CD8-APC A PE 10² 10¹ ¹ 10² B PE 10² 10¹ ¹ 10² GFP GFP 3

5 MACSQuant Analyzer 10 MACSQuant VYB 405 nm, 488 nm, 638 nm 405 nm, 488 nm, 561 nm FSC, SSC8 FSC, SSC8 Violet laser : VioBlue, Hoechst dyes, Pacific Blue, Pacific Orange, VioGreen Blue laser : FITC, PE, PI, PE-Cy5.5, PE-Cy7, PerCP-Vio700, VioBright FITC, Red laser : APC, APC-Cy7, APC-Vio770 Violet laser : VioBlue, Hoechst, CFP, Pacific Orange, GFP, YFP, VioGreen Blue laser : GFP, FITC, PI, Lss-Kate, VioBright FITC Yellow laser : PE, mcherry, dsred, Texas Red, PE-Cy5, APC,mKate,PE-Cy7 PE-Vio770, APC-Vio ,000 events / second 10,000 events / second MACS +/- 5 %; (CV) <5 % 5 decade logarithmic scales MESFs: <200 FITC; <100 PE;<150 APC CV: <5% MACS +/- 5 %; (CV) <5 % 5 decade logarithmic scales MESFs: <200 FITC; <100 PE;<150 APC CV: <5% 25 μl 25 μl 4 MACS 4 MACS 1 96 well 1 96 well 5mL96well : 5 / 15 / 50mL 5mL96well : 5 / 15 / 50mL 2 2 Y-Z Y-Z 2 10 ml / minute 2 10 ml / minute Microsoft Windows / MACSQuantify Microsoft Windows / MACSQuantify 4 USB 2.0, 2 HDMI, VGA, LAN 4 USB 2.0, 2 HDMI, VGA, LAN MACSQuant Data (MQD), Flow Cytometry Standard (FCS) FCS3.0 MACSQuant Data (MQD), Flow Cytometry Standard (FCS) FCS W 100 V 350 W 100 V 50 kg 50 kg (W D H) mm (W D H) mm MACSQuant X 405 nm, 488 nm, 638 nm FSC, SSC8 Violet laser : VioBlue, Hoechst dyes, Pacific Blue, Pacific Orange Blue laser : FITC, PE, PI, PE-Cy5.5, PE-Cy7, PerCP-Vio700, VioBright FITC Red laser : APC, APC-Cy events/second 150 μl/min <15 min per 96 wells 10 μl 5 ml 0.01% 384 well96 well 5 mlchill 5 Rack 4

6 automacs Pro Separator automacs Pro Separator automacs ProMACS automacs Pro 11,000 autolabeling 5

7 CD14 + CD4-PE CD34-PE Side scatter Forward scatter CD45-FITC Forward scatter CD4 + CD34 + CD4-PE CD34-PE CD14-PE Forward scatter CD45-FITC CD45-FITC automacs Pro Starting Kit 5,600,000 automacs Pro Separator MACS MiniSampler MACS Reagent Rack 4 MACS Cooling Tube Rack Chill 5 Chill 15 Chill automacs 10 automacs 4 automacs Pro Buffer Combination 1 1 / ml / ml 605 mm 455 mm mm 25 kg VAC Hz 200 VA * * 1 MACS MiniSampler 2 27 mm62 mm MACS Cooling Tube Racks MACS Cooling Tube Rack Chill ml 6 5 ml 2.5 ml Chill 15 Chill ml 5 5 ml 6 50 ml 3 15 ml 3 5 ml 5 15 ml 12.5 ml ml 50 ml

MultiMACS TM MACS MultiMACS Cell24 Separator Plus MultiMACS Cell24 Separator Plus MACS Whole Blood MicroBeads MultiMACS Cell24 Separator Plus MultiMACS Cell24 Separator

24-well Deep Well Plate, sterile packed 1 130-098-637 Single-Column Adapter MultiMACS 5 ml (130-095-331) Column Blank Holder Column Blanks 9 Tubing Power cord Null modem

8 MultiMACS TM MACS MultiMACS Cell24 Separator Plus MultiMACS Cell24 Separator Plus MACS Whole Blood MicroBeads MultiMACS Cell24 Separator Plus MultiMACS Cell24 Separator Plus 2,800,000 MultiMACS Cell24 Separator MACS Elution Station Elution Chamber Base Elution Chamber Lid I Elution Chamber Lid II Multi-24 Cell Separation ( ) 2 24-well Deep Well Plate, sterile packed Single-Column Adapter MultiMACS 5 ml ( ) Column Blank Holder Column Blanks 9 Tubing Power cord Null modem cable High power CAN cable MultiMACS Cell24 Separator W D H MACS Elution Station W D H mm 11 kg VAC Hz 200 W mm 4.6 kg 24 VDC 21 W 7

M96thermo Separator W D H MultiMACS M96 Separator MultiMACS M96thermo Separator 230 435 230mm 11 kg 100-240 VAC 50-60 Hz 200 W 365

9 MultiMACS M96 Separator MultiMACS M96thermo Separator MultiMACS M96 Separator MultiMACS M96thermo Separator MultiMACS M96 Separator MultiMACS M96 Separator touch-screen control panel tip-touch plate power cord 1,780,000 MultiMACS M96 Separator / M96thermo Separator W D H MultiMACS M96 Separator MultiMACS M96thermo Separator mm 11 kg VAC Hz 200 W 365 W MultiMACS M96thermo Separator 1,980,000 MultiMACS M96thermo Separator touch-screen control panel tip-touch plate power cord 8

Heaters gentlemacs Octo Dissociator gentlemacs Octo Dissociator with

10 gentlemacs TM gentlemacs Dissociator gentlemacs Dissociator gentlemacs Octo Dissociator gentlemacs Octo Dissociator gentlemacs Octo Dissociator with Heaters gentlemacs Octo Dissociator gentlemacs Octo Dissociator with Heaters 36sec 37sec 37 C 30min 37 C 30min 1 10min 10min 30min 37 C 1hr 37 C 1hr 3 9

11 gentlemacs Tubes C tube M tube WEB WEB 4 WEBCODE c a a d gentlemacs OctogentleMACS Octo with Heaters gentlemacs Octo Dissociator with Heaters gentlemacs Octo Dissociator 2,980,000 2,680, , gentlemacs Starting Kit gentlemacs Octo Dissociator gentlemacs Octo Dissociator 1 with Heaters Power Cord Individual heating units 8 User Manual Power Cord User Manual gentlemacs Dissociator C TubesM Tubes 2 Power Cord User Manual gentlemacs Protocols L W H 465 mm 285 mm 185 mm 465 mm 285 mm 185 mm 347 mm 215 mm 195 mm 11 kg 11 kg 4.7 kg 450 W 450 W 320 W VAC Hz VAC, 50/60 Hz VAC Hz 20-4,000 rpm 200-4,000 rpm 200-4,000 rpm 4-30 C 4-35 C 4-35 C 4-35 C Heating unit 37 C C Tubes 50 M Tubes 50 C Tubes 25 + M Tubes

MACSmix TM Tube Rotator MACSmix TM Tube Rotator 178,000 MACSmix AC 0.5 2.

12 MACSmix TM Tube Rotator MACSmix TM Tube Rotator 178,000 MACSmix AC ml ml NiMH 4 L x W H) AC mm 430 g 4 rpm 8 rpm12 rpm 12 rpm(30 )- ( / 5 ) 12 VDC 25 W VAC Hz NiMH 4 / 2 42 C / 0 % 85 % MACSQuant P1 Analyzer 10 VYB X MultiMACS TM Cell24 Separator P7 gentlemacs TM P9 Dissociator Octo Dissociator Octo with Heaters automacs Pro Separator P5 MultiMACS TM M96 P8 Separator thermo Separator MACSmix TM P11 FAX Miltenyi Biotec MACS MACSQuant automacs VioVioBlue Miltenyi Biotec GmbHMACSQuantify MACSmix MultiMACS gentlemacs VioGreen PE-Vio770 APC-Vio770VioBrightMiltenyi Biotec GmbH dE150

MACSQuant - 高機能・利便性を誇るフローサイトメーター

MACSQuant - 高機能・利便性を誇るフローサイトメーターフローサイトメトリーソリューション Discover MACSQuant Best in class MACSQuant - いつも実験の中心にスムーズな実験を実現するミルテニーバイオテク社の製品群細胞の絶対数測定 MACSQuant Analyzer 10 - シンプルで柔軟精密性再現性正確性の高い測定 p.6 高性能なフローサイトメーター p.4 MACS モレキュラー解析 MultiMACS