研究会プログラム.doc
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1 10 6 0:30-0:40 Session 0:40-1:10 Rac Michelle Lennartz 1:10-1:40 nnos 1:40-2:10 2:10-2:20 Session 2:20-2:50 2:50-3:20 NMDA NR1 3:20-3:50 GABA 3:50-4:00 Session 4:00-4:30
2 4:30-5:00 Vacuolar-type H + -ATPase 5:00-5:30 ph 5:30-6:00 6: Session 8:45-9:15 KCNQ/M PIP2 PKC 9:15-9:45 HERG 9:45-10:15 Ca2+ TRPM2 10:15-10:25 Session 10:25-10:55 TRPV2 10:55-11:25 PLC-zeta Ca2+ 11:25-11:55 Ca2+ 11:55-12:25
3 Rac Michelle Lennartz Albany Medical College NADPH Rac1: 6 ; Rac3: 4 ; Rac2: 3 oxidase (Nox2, p22 phox ) PB GFP (p47 phox, p67 phox, p40 phox, Rac) PB small GTPase Rac protein lipid overlay assay Rac1 PB PI(3)P PI(4)P PI(5)P p47 phox -p67 phox -p40 phox PI(3,4,5)P3PA Rac2 PB Rac2 Rac (Rac(Q61L)) GFP Rac (1, 2, 3) FcγR PB Rac PB Rac C 10 polybasic region (PB) GFP Rac Rac1 GFP Rac Rac PB Rac2 PB Rac3Rac2 C PB PB
4 nnos 1) 1) 2) 2) 3) 3) 1) MD L-Arginine (1 mm) (NO) NO TGF MD NaCl ([NaCl]l) TGF NO NO BAPTA-AM (Ca 2+ chelator) 50 µm 7-nitroindazole (nnos ) [NaCl][Na + ] (nnos) [Cl - ] [Na + ] NO NO MD (NE-MD) [NaCl]furosemide (Na + -K + -2Cl - Na + -H + MD nnos )amiloride (Na + -H + furosemide [C l - ]l ) nnos (Western blotting)no (NO ) Ca 2+ ph nnos furosemide
5 ( ) UV EPSC (VGLUT) Trypan bluevglut EPSC 5.1 H calyx of H + -ATPase (V-ATPase) bafilomycin A1 Held UV VGLUT 2
6 1,2, JST ICORP/SORST (PREGS) PREGS PREGS LTP 2 NMDA LTD LTP 1Hz LTD, 100Hz LTD EPSP S PREGS PREGS
7 NMDA NR1 NMDA NR1 3 ACPCACBC cycloleucine ACPCACBC NR1NR2B NMDA 80% 42% NR1 cycloleucine NR1 Non-NMDA GluR2 cycloleucine NR1 GluR2 2
8 GABA mglur G GPCR mglur Ca 2+ o mglur1 (5) GABABR Ca 2+ o GABABR Gi/o (1) GABABRmGluR1 Ca 2+ o GABABR 1 mglurmglur1 mglur1 mglur1 (2, 3) Ca 2+ o mglur1 GPCR GPCR co-factor 1.Kubo Y, Miyashita T, Murata Y (1998) Science 279, , mglur1 B GABA GABABR Ca 2+ o GPCR (4) Ca 2+ ogababr 2. Tabata T, Aiba A, Kano M (2002) Mol Cell Neurosci 20, , 3. Tabata T, Kano M (2004) Mol Neurobiol 29, , 4. Kulik A, Nakadate K, Nyiri G, Notomi T, Malitschek B, Bettler B, Shigemoto R (2002) Eur J Neurosci 15, , 5. Tabata T, Araishi K, GABABR Hashimoto K, Hashimotodani Y, Van der Putten H, Bettler B, Kano Ca 2+ o M (2004) PNAS 101,
9 pairing NMDA F-actin PA-GFP-actin F-actin
10 Vacuolar-type H + -ATPase ATP V-ATPase DCCD ruffled border H + V1 actin vacuolar-type H + -ATPase (V-ATPase) H + ATP V-ATPase H + actin filament membrane sectorvoatpase catalytic sector (V1) V-ATPase Vacuole V-ATPase H + ATP 0 mv DCCD H + V-ATPase holoenzyme ATP Vo/V 1 diassembly Vo H +
11 ph 1) 2) 1) 1 2) ph Na + /HCO3-4 (NBC4g) NBC4g NBC4c camp RNA NBC4 NBC4
12 1 1, 2, (1) lipid raft lipid raft Transgenic lipid raft 1 m LAT lipid raft CP /GFP lipid raft LAT GFP raft LAT CP LAT CP /GFP MCD cholesterol Transgenic LAT CP /GFP lipid raft lipid raft lipid raft lipid tyrosine raft lipid raft (2) - - RanGTP 30 Ran 8 / Mda 10nM100nM
13 KCNQ/M PIP2PKC 1,2 1,2 1..SORST,JST KCNQ/M Gq KCNQ/M KCNQ/M M 10 M oxo-m M1 KCNQ - (G-V ) PKC PMA KCNQ2 G-V 17.5mV 10 M PIP2 wortmannin PIP 2 PKC G-V PKC PIP 2 M PIP 2 PIP2 KCNQ/M PKC KCNQ M1 5 G-V PKC
14 HERG K 1) 3) 2 2) 3) HEK293 HERG 10 mm [K + ] HERG 500 mosm1500 mosm n [K + ]100 mm n 1 3 1) 1 2) -0.7 mv/? Osm n K [K + ]100 mm n 0.9 KcsA HERG
15 Ca 2+ TRPM2 TRPM2 (H2O2) Ca 2+ TRPM2 Ca 2+ TRPM2 TRPM2 TRPM2 extracellular signal-regulated kinase (ERK) NAD + ADP-ribose TRPM2 TRPM2 U937 C Nudix motif H2O2 IL-8 TRPM2 TRPM2 KO TRPM2 KO TRPM2 TRPM2 TRPM2 H2O2 TRPM2 Ca 2+
16 TRPV2 TRPV2 TRPV TRPV2 sirna Ca 2+ TRPV2 TRPV2 fmlp fmlp PI- fmlp Cs + TRPV ruthenium red TRPV2siRNA Cs + fmlp fmlp Ca 2+ ruthenium red TRPV2 TRPV2 TRPV2 Ca 2+ Ca 2+ ruthenium red
17 PLC-zeta Ca 2+ phospholipase C-zeta (PLCζ ) Ca 2+ portion) PLCζ Venus RNA 30 Ca 2+ Ca 2+ PLCζRNA PLCζ PLCζ N EF-hand domain X Y catalytic domain C C2 domain X Y PLC (hinge point mutation EF-hand domain N 10 Ca 2+ Ca 2+ PLCζ RNA EF-hand domain Ca 2+ -binding site
18 Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ IP 3 Ca 2+ IP 3 Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+ Ca 2+
19 Total Internal Reflection Fluorescence Microscopy: TIRFM 2 nm SN TIRFM TIRFM TIRF
Signal Receptor Ion Channel Transmitter Energy transducion and transport Receptor A signal Energy transduction Cross talk Nuclear Cascades B signal As
) " " " " 21 Signal Receptor Ion Channel Transmitter Energy transducion and transport Receptor A signal Energy transduction Cross talk Nuclear Cascades B signal Assembly ATP Transport Genetic information
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1003shinseihin.pdf
1 1 1 2 2 3 4 4 P.14 2 P.5 3 P.620 6 7 8 9 10 11 13 14 18 20 00 P.21 1 1 2 3 4 5 2 6 P7 P14 P13 P11 P14 P13 P11 3 P13 7 8 9 10 Point! Point! 11 12 13 14 Point! Point! 15 16 17 18 19 Point! Point! 20 21
株主通信：第18期 中間
19 01 02 03 04 290,826 342,459 1,250,678 276,387 601,695 2,128,760 31,096 114,946 193,064 45,455 18,478 10,590 199,810 22,785 2,494 3,400,763 284,979 319,372 1,197,774 422,502 513,081 2,133,357 25,023
ワタベウェディング株式会社
1 2 3 4 140,000 100,000 60,000 20,000 0 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 5 6 71 2 13 14 7 8 9 10 11 12 1 2 2 point 1 point 2 1 1 3 point 3 4 4 5 6 point 4 point 5 point 6 13 14 15 16 point 17
untitled
1 2 3 4 5 6 7 Point 60,000 50,000 40,000 30,000 20,000 10,000 0 29,979 41,972 31,726 45,468 35,837 37,251 24,000 20,000 16,000 12,000 8,000 4,000 0 16,795 22,071 20,378 14 13 12 11 10 0 12.19 12.43 12.40
