＜資料５＞ 除草剤グリホサート耐性クリーピングベントグラス(cp4 epsps, Agrostis stoloniferaL.) (ASR368, OECD UI: SMG )申請書等の概要
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- ありさ いざわ
- 5 years ago
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1 (cp4 epsps, Agrostis stolonifera L.) (ASR368, OECD UI: SMG )
2 (cp4 epsps, Agrostis stolonifera L.) (ASR368, OECD UI: SMG-368ØØ-2) (1) 7, m (2) (3) 1
3 (4) 3,196 2 (1) (2) 5mm () (3) (4) (5) (6) (7) (8) (1)(6) (9) 2
4 (1),. creeping bentgrass. Agrostis stolonifera L. (Gramineae)(Agrostis ) (Agrostis stolonifera L.)(2n=4x=28) Backspin A. stolonifera A. stolonifera Agrostis 7 (1 ) (A. flaccida Hack.) (A. mertensii Trin.) (A. hideoi Ohwi) (A. clavata Trin.) (A. clavata Trin. var. nukabo Ohwi) (A. scabra Willd.) (A. nipponensis Honda) ( ) Agrostis ()(A. stolonifera L.) ()( A. alba L.) 2 3
5 Agrostis ()Agrostis () ( )( ) Agrostis (2) Agrostis (A.capillaris) (A. canina) ( 14 Agrostis 118 4
6 cm 15 20cm 1030g/m 2 810g/ m (3) 5100cm 200cm 130cm 2 3mm ph ph cm 25 5
7 1 Agrostis Polypogon Agrostis Agrostis (F1) (F1)(F1) Polypogon Agrostis 42m m (1) (cp4 epsps, Agrostis stolonifera L.) (ASR368, OECD UI: SMG-368ØØ -2) ( ) 1(p9) 1(p9) 6
8 5- -3-(EPSPS)(E.C ) EPSPS cp4 epsps CP4 EPSPS EPSPS D-- -7-(3-deoxy-D-arabino-heptulosonate-7-phosphate, DAHP) DAHP EPSPS DAHP EPSPS EPSPS 40 EPSPS ()/ EPSPS EPSPS (PEP)-3-(S3P)EPSP (Pi) EPSPS S3P S3P CP4 EPSPS 7
9 (2) (Escherichia coli) puc 119 (nptii ) ori 1(p9) PV-ASGT08 9,308bp (3) nptii puc119 cp4 epsps ([P-ract1/ract1 intron]-[ctp2]-[cp4 EPSPS]-[NOS 3 ][e35s]-[zmhsp70]-[ctp2]-[cp4 EPSPS]-[NOS3 ]) PV-ASGT08 PV-ASGT08 HindIII DNA (PV-ASGT08L)cp4 epsps 2 CP4 EPSPS 8
10 1 ASR368 PV-ASGT08 cp4 epsps P-ract1 1 ract1 intron CTP 2 cp4 epsps epsps EPSPS N Agrobacterium CP p4-5 NOS 3 Agrobacterium tumefaciens T-DNA (NOS) 3 mrna cp4 epsps E35S (CaMV) 35S ZmHsp70 Intron CTP2 cp4 epsps (heat shock protein) ZmHsp70 epsps EPSPS N Agrobacterium CP p4-5 NOS 3 Agrobacterium tumefaciens T-DNA (NOS) 3 mrna Ori NptII E. coli puc119 E. coli Tn5 neomycin phosphotransferase type II(NPTII) 9
11 Sph I Sph I Nde I Sph I Sph I Sma I Hind III Nde I Hind III 5 flank P-ract ract intron ctp2 cp4 NOS 3 P-e35S ZmHSP70 intron ctp2 cp4 NOS 3 3 flank 1. ASR368DNA 10
12 DNA PV-ASGT08L Backspin 1997 PV-ASGT08 HindIII DNA PV-ASGT08L Backspin CP4 EPSPS (4) cp4 epsps p10 1 CP4 EPSPS 77.1g/g fwt g/g fwt g/g fwt g/g fwt g/g fwt CP4 EPSPS (5) 10g DNA PCR (6) cp4 epsps CP4 EPSPS
13 R0 B99061R R1 R0 PenneaglePennlinks ProvidencePutterSouthshore F1 PenneaglePennlinksProvidencePutterSouthshore R0 RS(Roundup Susceptible) R1 F1 1 1 BackspinCrenshowPenn-4SR ( 1,000 ) R1RS R0F1 B99061R
14 () 1,000 R1 RS 2002 R0 Penneagle PennlinksProvidencePutterSouthshore 14 25/ R0 cp4 epsps R0 R1( ) RS() 1:1 R0 4 cp4 epsps 32R R1 5 () 13
15 R0F1 B99061R 3 ()() R0 B99061R m50m185m274m354m Agrostis ( 12 ) Polypogon (3 ) 2m 48.87% 354m 0.15% 50m Agrostis 11 Polypogon 3 2m 50m 2m 2.68%(p. fugax)50m 0.026%(A. capillaris) 185m 185m Agrostis 14
16 CP4 EPSPS 2-(1)-- CP4 EPSPS EPSPS ( )/ CP4 EPSPS (1) (2) (1) 7, m (2) (3) (4) 3,196 (1) (2) 5mm ( ) 1 15
17 (3) (4) (5) (6) (7) (8) (1)(6) (9) (3) (4) (5)
18 (1) 2-(6)- 3-(5) () (2) (3) (4) 17
19 (1) CP4 EPSPS 2-(1)-- CP4 EPSPS EPSPS () / CP4 EPSPS (2) (3) (4) 18
20 (1) (Agrostis stolonifera L.) Agrostis Agrostis (F1)(F1) (F1) Polypogon Agrostis Polypogon (354m) 185m (F1) 2m 50m (F1) (F1)(F1) (F1) 1-(1)-3--Agrostis (A. flaccida Hack.)(A. mertensii Trin.)(A. hideoi Ohwi)(A. scabra Willd.)(A. nipponensis Honda)(A. clavata Trin.)(A. clavata Trin. var. nukabo Ohwi)()(A. alba L.) Polypogon (P. monspeliensis (L.) Desf.) (P. fugax Steud.) 2 10 (1 ) 19
21 2-(6)-- 185m Agrostis Polypogon (2) (3) (4) 20
22 Agrostis Polypogon 21
23 F (cp4 epsps, Agrostis stolonifera L.) (ASR368, OECD UI: SMG-368 ØØ -2) () 22
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SMG-368ØØ -2 1 (1),. creeping bentgrass. Agrostis stolonifera L. (Gramineae)(Agrostis ) (Agrostis stolonifera L.)(2n=4x=28) Backspin A. stolonifera A. stolonifera Agrostis 7 (1 ) (A. flaccida Hack.) (A.
高オレイン酸ダイズ（ GmFad2-1, Glycine max (L.) Merr.）(260-05, OECD UI :DD- Ø26ØØ5-3) 申請書等の概要
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9 3 12 17 15 19 10 19 13 16 16 17 11 17 14 16 17 19 4 4 5 11 11 13 1,739m 1,722m 25km 17km 6,817 1,052m 1,018m 14 1,858,070 668,446 35 1,101,872 59 4 5 6 22 1889 7 20 52 228 1619 5.3.17 32.5 130.6
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和県監査H15港湾.PDF
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第18回海岸シンポジウム報告書
2011.6.25 2011.6.26 L1 2011.6.27 L2 2011.7.6 2011.12.7 2011.10-12 2011.9-10 2012.3.9 23 2012.4, 2013.8.30 2012.6.13 2013.9 2011.7-2011.12-2012.4 2011.12.27 2013.9 1m30 1 2 3 4 5 6 m 5.0m 2.0m -5.0m 1.0m
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