Immunological parameters such as lymphocyte subsets, blastogenesis, natural killer (NK) activity of peripheral blood lymphocytes, and serum interferon

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1 Immunological parameters such as lymphocyte subsets, blastogenesis, natural killer (NK) activity of peripheral blood lymphocytes, and serum interferon activity were investigated on 40 oral cancer patients composed of 20 good prognosi cases without metastasis (good group) and 20 bad prognosis/metastasis-positive cases (bad group). The results obtained were as follows. 1) While each lymphocyte subset proportion in good group was not significantly different from that in healthy controls, Leu3a+8+ cell proportion in bad group was significantly lower (p<0.05) than the controls', and statistically significant decreasing cells with chemo/radiotherapy was observed in good group. (p<0.001) of Leu3a+8+ 2) Lymphocyte blastogenesis was correlated with Leu3a+8+ cell proportion (p<0.001), and blastogenic response in bad group was inferior to the controls' lymphocyte response. 3) No significantly different NK activity could be observed among two patient groups and healthy controls, but serum IFN activity in good group was significantly higher than that in controls (p<0.01). From the above, it was revealed that immune condition relates with the clinical feature of oral cancer patients, and Leu3a+8+ cell proportion appears especially useful for the evaluation of the patient's status.

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10 1) Ueda, T., Kohda, H., Ibayashi, Y. et al. : Functional analysis of mononuclear cells infiltrating into tumors. J. Immunol., 135 : , ) Uchida, A., Micksche, M.: Lysis of fresh human tumor cells by autologous large granular lympocytes from peripheral blood and pleural effusions. Int. J. Cancer, 32 : 37-44, ) Biron, C.A., Welsh, R.M. : Blastogenesis of killer cells during viral infection in vivo. J. Immunol., 129 : , ) Shirakawa, F., Tanaka, Y., Oda, S. et al. Immunosuppressive factors from adult T-cell leukemia cells. Cancer Res., 46 : , ) Kedar, E., Weiss, D.W. : The in vitro generation of effector lymphocytes and their employment in tumor immunotherapy. Adv. Cancer Res., 38 : , ) Boldt, D.H., Mills. B.J., Gemlo, B.T. et al. Laboratory correlates of adoptive immunotherapy with recombinant interleukin-2 and lymphokine-activated killer cells in humans. Cancer Res., 48 : 4409^-4416, ) Owen-Schaub, L.B., Gutterman, J.U., Grimm, E.A. : Synergy of tumor necrosis factor and

11 13) Ohno, T., Kanoh, T., Suzuki, T. et al.: Comparative analysis of lymphocyte phenotypes between carriers of human immunodeficiency virus (HIV) and adult patients with primary immunodeficiency using two-color immunofluorescence flow cytometry. Tohoku J. Exp. Med., 154 : , subpopulation of Leu-2+cells that suppresses B cell differentiation. J. Immunol., 133 : , al.: Dissection of immunoregulatory sub- populations of T lymphocytes within the helper and suppressor sublineages in man. J. Immunol., 129 : , ) Kansas, G.S., Engleman, E.G.: Phenotypic identification of suppressor-effector, suppres- sor-amplifier and suppressor-inducer T cells of B cell differentiation in man. Eur. J. Im- munol., 17 : , ) Salmon, M., Kitas, G.D., Bacon, P.A. : Pro- duction of lymphokine mrna by CD45R+ and CD45R- helper T cells from human peripheral blood and human CD4+ T cell clones. J. Immunol., 143 : , ) Handa, K., Suzuki, R., Matsui, H. et al. : Natural killer (NK) cells as a responder to interleukin 2 (IL2). J. Immunol., 130: , ) Kasahara, T., Hooks, J.J., Dougherty, S.F. et al. : Interleukin 2-mediated immune interferon (IFN-y) production by human T cells and T sell subsets. J. Immunol., 130: , ) Abo, T., Miller, C.A., Balch, C.M.: Characterization of human granular lymphocyte subpopulations expressing HNK-1 (Leu-7) and Leu-11 antigens in the blood and lymphoid tissues from fetuses, neonates and adults. Eur. J. Immunol., 14 : , ) Clement, L.T., Grossi, C.E., Gartland, G.L.: Morphologic and phenotypic features of the 23) Gatenby, P.A., Kansas, G.S., Xian, C.Y. et 26) Hirohata, S., Lipsky, P.E.: T cell regulation of human B cell proliferation and differentiation. J. Immunol., 142 : , ) Hedlund, G., Dohlsten, M., Sjogren, H.O. et al. : Maximal interferon-gamma production and early synthesis of interleukin-2 by CD4+ CDw29- CD45R- p80- human T lymphocytes. Immunology, 66 : , ) Damle, N.K., Childs, A.L., Doyle, L.V.: Immunoregulatory T lymphocytes in man. J. Immunol., 139: , 1987.

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