カズノコの栄養機能性.ppt
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- がんま にかどり
- 5 years ago
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5 R-COOH R CH -O-CO-R 2 1 CH-O-CO-R 2 R CH -O-CO-R CH -O-CO-R 2 1 CH-O-CO-R2 O CH -O-P-O-X 2 - O 4. R X HO
6 CH 3 (CH 2 ) 14 COOH 16 0 CH 3 (CH 2 ) 7 CH=CH(CH 2 ) 7 COOH 18 1 CH 3 (CH 2 ) 4 (CH=CH-CH 2 ) 2 (CH 2 ) 6 COOH CH 3 (CH 2 ) 4 (CH=CH-CH 2 ) 4 (CH 2 ) 2 COOH CH 3 CH 2 (CH=CH-CH 2 ) 3 (CH 2 ) 6 COOH CH 3 CH 2 (CH=CH-CH 2 ) 5 (CH 2 ) 2 COOH CH 3 CH 2 (CH=CH-CH 2 ) 6 CH 2 COOH
7 !"#$%&'()* +,-.'/ ,56'/7189:;<=4 CDEFG6HC6'4 IJKLM/NOB19PQ4 ISTLM/NOB19PQ UV(WX/4 YZ[\(]^_`ab\)*Zc(d+,-.'e4 Yfg9hiejklmn]^<=_dSTLop*
8
9
10
11
12 :DHA :EPA 0
13
14
15 UCP UCP UCP
16
17 TG Gs AC camp HSL PKA R Retinoids Heat FFA UCP1 PPAR mrna UCP1 CREB RXR PGC-1 PGC-1 PPAR RXR R PGC-1 T 3 R TR RXR EPA, DHA BAT
18 a 10 b (a:p<0.05; b:p<0.01)
19 b a mg/dl 40 mg/dl a a,b (a:p<0.05; b:p<0.01). 0
20 a DHA+EPA BAT EPA or DHA DNA PPARγ + PPARγ RXR PPRE 9-cis-Retinoic acid (Endogenous ligand for RXR) RXR Up-regulation Of UCP1 Target gene a (P<0.05)
21
22 100µM
23 GLUT DHA DHA (100µM) (50µM) EPA EPA (100µM) (50µM) DHA DHA (100µM) (50µM) Leptin (RT-PCR) EPA EPA (100µM) (50µM)
24
25
26 (mg/dl) (mg/dl) (mg/dl) (mg/dl) (mg/dl)
27
28
29 12 10 %
30
31
32
33 a 50 0
34 a a 20 0
35
36 (18:2n-6; LA) 2 (18:3n-3; α-ln) 3 (18:3n-6; γ-ln) 3 (20:4n-6; AA) 4 (20:5n-3; EPA) 5 (22:6n-3; DHA) CH=CH-CH2-CH=CH (RH) CH=CH-CH-CH=CH (R ) ROOH H O2, H
37
38 [DHAEPA]
39
40
41
42 16:0() 16:1n-7 18:1n-9() 18:1n-7 20:5n-3() 22:6n-3()
43
44 PA PA P PA,PA PA AA P PA,AA PA OA P PA,OA PA DHA P PA,DHA PA:16:0; OA:18:1n-9; AA:20:4n-6; DHA:22:6n-3
45 PA PA OA PA OA OA P Ethanolamine P Ethanolamine P PA,PA PA,OA OA,OA Ethanolamine PA PA PA LA AA DHA P Ethanolamine P Ethanolamine P PA,LA PA,AA PA,DHA Ethanolamine PA:16:0; OA:18:1n-9; LA:18:2n-6; AA:20:4n-6; DHA:22:6n-3
46 (%) PA,DHA PC PA,OA PA,PA PA,AA (hr) (%) OA,OA PE PA,OA PA,PA (hr)
47 (%) PC - - : - - :+Toc. - - :Toc.+ PA,PA - - :Toc.+ PA,OA - - :Toc.+ PA,AA - - :Toc.+ PA,DHA (hr) (%) 100 PE (hr) (Toc.) Toc. Toc.+ PA,LA Toc.+ PA,PA Toc.+ PA,DHA Toc.+ OA,OA Toc.+ PA,AA
48 DHA (%) DHA Toc.+ OA,OA +Toc. Toc.+ PA,PA (hr) Toc.+ OA,DHA (x10 4 ) Toc. Toc.+ OA,OA Toc.+ OA,DHA (hr) (Toc.)
49 CH 3 O CH 3 O O O CH 3 CH 3 n H OH HO
50
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