LSM5Pascal Ver 3.2 GFP 4D Image VisArt Carl Zeiss Co.,Ltd.

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1 LSM5Pascal Ver 3.2 GFP 4D Image VisArt

2 LSM5PASCAL V3.2 LSM5PASCAL SW3.2Axiovert200M SingleTrack 9 Multi Track 10,18 5 / ( OFF) 40

3 LSM5PASCAL V3.2 LSM5PASCAL ECU MO

4 ( ) On PC Ar HeNe ON PC Windows Log in HeNe 543nm G 90 HeNe 633nm R 405nm:V Page 1

5 Ar nm B Ar Power Enable ON 90 Standby on Power PC 5 Page 2

6 Pascal OnlineMode Start Online Mode Start Offline ModePC Start Offline Mode Online Mode Page 3

7 7 File Save/Load Exit Acquire Process 3D ViewAcquire D Macro MacroEditor Options Maintain Page 4

8 LSM5Pascal Acquire Page 5

9 Laser Control Page 6

10 Microscope Setup Acquire Micro PC VIS 7 Page 7

11 Transmitted Light ON/OFF Reflected Light ON/OFF Objective Reflector / Reflector Turret Page 8

12 Configuration LSM 2 Single Track Multi Track Acquire Config Single Track Single Track Store/Apply Apply Configuration B FITC G Rhodamin R Cy5 Page 9

13 Multi Track Single Track Multi Track Scan Control Acquire Scan Multi Track 1 Page 10

14 Scan Control 2 Scan Control Acquire Scan Mode Channel AOTF Page 11

15 Find / LSM Acquire Scan Channels Pinhole Pinhole Optical Slice Find Find Cont. Find Page 12

16 Find Saturation Scan Control Channels Cont. Stop Detector Gain Ample.Offset Ample.Offset ( ) Stop Page 13

17 Scan Mode Mean Number Line Frame Single Total ModeLine Frame Method Mean Sum Number Page 14

18 Scan Control New : Find : Fast XY : Scan Speed10 Single : 1 1 Stop : Cont. Cont. Continue : Stop Page 15

Mode Objective Lens Image Size Image Size Speed 13 12 Pixel Depth, Scan

19 Mode Objective Lens Image Size Image Size Speed Pixel Depth, Scan Direction Scan Average 8 or 12 bit Frame Line Zoom, Rotation Offset Channnels Page 16

20 Channel Data Pinhole[m] Optical Slice Z Airy Unit Optical Slice Airy 1 Detector GainAmplOffsetAmplGain & Excitation MOTF MOTF 488nm 510 Page 17

21 Multi Track ( ) LSM5PASCAL Single Track Multi Track 2 Single Track Rhodamine FITC Multi Track 1 Single Track Find Page 18

22 Aquire Config Multi Track Multi Track Config Single Track Apply Find Single Track Find1 Multi Track Pinhole Find Find Page 19

23 Page 20

24 LSM5Pascal V3.2 Page 21 5 LSM5PASCAL 2 Windows Zeiss - Image Browser Export 2 A A: 3.5 C C: Windows LSM D D: I I: CD-RW CD NeroBurningROM : MO 640MB Windows C/D LSM510 D MO HDD MO MO

25 5 File New -.mdb fig.1.mdb.lsm fig.2 Open fig.1 Page 22

26 File Save OK Save 5 Open - /.mdb.lsm mdb LSM510 ImageBrowser Page 23

27 File Open Open Reuse Apply Save Page 24

28 6 TIFF/JPEG etc Export File Export TIFF Tagged Image File Save Image Type Image Type Raw data single series Series 000.tif 001.tif 002.tif 3 Contents of the Image Window single series pixel Full resolution image window single series Page 25

29 Import File Import Single Series All Supported File Open Page 26

30 6 3D 6 Z Sectioning Scan 6 Sectioning Scan Control Z stack Z setting Page 27

31 Mark First/Last XY Cont. Mark First Mark Last Scan Stack Z Size Page 28

32 (2) Z Slice Optimal Interval Optical Interval Num Slice Start Start Average Page 29

33 6 SliceAnim OrthoCut Depth Coding 1 Rotation Stereo Slice Anim Gallery Slice Anim Slice Anim Slice Gallery Page 30

34 Ortho Cut 2 Ortho Cut 5 Page 31

35 Depth coding 3D view DepthCod ThresholdContrast Brghtness Apply Scale Bar Page 32

36 3D Projection Sectioning 1 3D View Projection First Angle0 First Angle Number Projections 32 Difference Angle Apply Anim 1 First Angle Number Projection Difference Angle Page 33

37 Stereo Image 3D 3D Stereo Mode 2 Red/Green Image Split Image Right Left Angle 510 Apply Number Image Difference Angle Page 34

38 Page 35

39 1 9 HAL 2 10 FL Page 36

40 / HAL on/off ON H H Page 37

41 2 DIC DIC 3DIC DIC DIC LSMDIC LSM Page 38

42 Page 39

43 ON HAL on/off OFF FL on/off Page 40

44 Page 41

Q&A目次.PDF

Q&A目次.PDF LSM5Pascal Q A Q1 Timeseries Q2 Timeseries Q3 Q4 Q5 1 Q6 Image Browser Q7 Q8 Q9 Acquire Time Series Manual Time 1 Unit sec 1 7 Cycle Delay n n 1 Time Interval n n 1 Options Settings TimeSeries Page 40