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1 1 SOP

2 Copyright 2007 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. eppendorf, Eppendorf Reference, Eppendorf Research, Eppendorf Research plus, Multipette plus, Multipette stream/xstream, Varipette, Varispenser, Biomaster, Combitip and PICASO are registered trademarks of Eppendorf AG, Hamburg, Germany. AESOP13640-/0310

3 Systematic error Randam error / Xplorer V

4 7.2.5 V M M Xplorer F F Z Z F F V V Xplorer M M Xplorer stream/xstream EN ISO

5 1 1 EN ISO 8655 Volume-measuring equipment with pistons EN ISO μL * 1μL~10μL >10μL~ ~100μL >100μL~1,000μL >1mL~10mL >10mL~ ~200mL mg * 5

6 μL PICASO EN ISO 8655, Part C 30 C 50% ISO 3696 grade

7 /4981 1/ stream/xstream % 10% % 10% / stream Xstream

8 ~3mm 3. 1~3 V 1-10 ml V 1-10 ml % 8

9 Systematic error X Z n n = Z 29 Z =Systematic error e s μl e X X Systematic error% e 100 X X X 3.2 Random error n =Random error (s) s X Z X n 1 CV Random error CV % S X

10 C / / mL 10mL mL 10mL

11 / / stream Xstream Xplorer Xplorer PUSH UP TO RELEASE %

12 μL

13

14 ISO V 3120 M 3122 V 3111 M

15 C ±0.5 C EN ISO 8655, Part 6 V 3120 M 3122 V 3111 M Z % No

16 No B

17 V 3111 No D μL μL 2-20μL μL μL 100-1,000μL 500-5,000μL 1-10mL 0.1μL 0.5μL 1μL 5μL 10μL 50μL 250μL 510μL

18 V No

19 M 3114 No D μL μL μL 0.5μL 5μL 10μL

20 M C Xplorer Xplorer Xplorer 20

21 No B μL 10 20μL 25 50μL 100μL μL 500 1,000μL 1,500 2,000 2,500μL 0.5μL 1μL 2.4μL 5μL 12μL 46μL 118μL 21

22 F 3112 No D μL 20μL 25μL 50μL 100μL 200μL 250μL 500μL 1,000μL 0.8μL 0.8μL 0.8μL 0.8μL 0.8μL 38μL 38μL 38μL 38μL F

23 mg µl 1. μl

24 8 Z 24 8 Z 8.1 Z Z μl/mg EN ISO 8655 C kpa

25 9 9 20~25 C ±0.5 C 10 ISO μl ,000 1,500 2,000 2,500 Systematic error (inaccuracy) % μl 20μL 20μL, L ±2.5 ±0.025 ±2.0 ±0.04 ±1.5 ±0.075 ±1.0 ± μL ±1.0 ±0.8 ±0.8 ±0.7 ±0.6 ±0.6 ±0.6 ±0.6 ±0.6 ±0.6 ±0.6 ±0.6 1,000μL 2,500μL ±0.1 ±0.16 ±0.2 ±0.35 ±0.6 ±1.2 ±1.5 ±3 ±6 ±9 ±12 ±15 Random error (imprecision) % μl ±1.8 ±1.2 ±0.8 ±0.5 ±0.5 ±0.3 ±0.3 ±0.3 ±0.2 ±0.2 ±0.2 ±0.2 ±0.2 ±0.2 ±0.2 ±0.2 ±0.018 ±0.024 ±0.04 ±0.05 ±0.05 ±0.06 ±0.075 ±0.15 ±0.2 ±0.4 ±0.5 ±1 ±2 ±3 ±4 ±5 25

26 F 3112 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 200μL 10 ±1.2 ±0.12 ±0.6 ± ±1.0 ±0.2 ±0.3 ± ±1.0 ±0.25 ±0.3 ± ±0.7 ±0.35 ±0.3 ± ±0.6 ±0.6 ±0.2 ±0.2 1,000μL 200 ±0.6 ±1.2 ±0.2 ± ±0.6 ±1.5 ±0.2 ± ±0.6 ±3 ±0.2 ±1 1,000 ±0.6 ±6 ±0.2 ±2 26

27 F 3121 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 20μL 40mm 10 ±1.2 ±0.12 ±0.6 ± μL, L 46mm 20 ±0.8 ±0.16 ±0.3 ± μL 53mm 10 ±1.2 ±0.12 ±0.6 ± ±1.0 ±0.2 ±0.3 ± ±1.0 ±0.25 ±0.3 ± ±0.7 ±0.35 ±0.3 ± ±0.6 ±0.6 ±0.2 ± ±0.6 ±1.2 ±0.2 ±0.4 1,000μL 71mm 200 ±0.6 ±1.2 ±0.2 ± ±0.6 ±1.5 ±0.2 ± ±0.6 ±3 ±0.2 ±1 1,000 ±0.6 ±6 ±0.2 ±2 27

28 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 10μL μL 0.25 ±12.0 ±0.03 ±6.0 ± ±2.5 ±0.031 ±1.5 ± ±1.4 ±0.035 ±0.7 ± μL 20μL 20μL, L μL 1 ±2.5 ±0.025 ±1.8 ± ±1.5 ±0.075 ±0.8 ± ±1.0 ±0.1 ±0.4 ± μL 20μL, L 2-20μL 2 ±3.0 ±0.06 ±2.0 ± ±1.0 ±0.1 ±0.5 ± ±0.8 ±0.16 ±0.3 ± μL 2-20μL 2 ±5.0 ±0.1 ±1.5 ± ±1.2 ±0.12 ±0.6 ± ±1.0 ±0.2 ±0.3 ± μL 10 ±3.0 ±0.3 ±0.7 ± ±1.0 ±0.5 ±0.3 ± ±0.8 ±0.8 ±0.15 ± μL 50 ±1.0 ±0.5 ±0.3 ± ±0.9 ±0.9 ±0.3 ± ±0.6 ±1.2 ±0.2 ±0.4 1,000μL μL 50 ±1.4 ±0.7 ±0.3 ± ±1.1 ±1.1 ±0.3 ± ±0.6 ±1.5 ±0.2 ± ,000μL 100 ±3.0 ±3 ±0.3 ± ±1.0 ±5 ±0.2 ±1 1,000 ±0.6 ±6 ±0.2 ±2 2,500μL 500-2,500μL 500 ±1.5 ±7.5 ±0.3 ±1.5 1,000 ±0.8 ±8 ±0.2 ±2 2,500 ±0.6 ±15 ±0.2 ±5 28

29 V 3111 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 10μL μL 0.25 ±12.0 ±0.03 ±6.0 ± ±2.5 ±0.031 ±1.5 ± ±1.4 ±0.035 ±0.7 ± μL 20μL 20μL, L μL 1 ±2.5 ±0.025 ±1.8 ± ±1.5 ±0.075 ±0.8 ± ±1.0 ±0.1 ±0.4 ± μL 2-20μL 2 ±5.0 ±0.1 ±1.5 ± ±1.2 ±0.12 ±0.6 ± ±1.0 ±0.2 ±0.3 ± μL 10 ±3.0 ±0.3 ±1.0 ± ±1.0 ±0.5 ±0.3 ± ±0.8 ±0.8 ±0.2 ± μL 20 ±2.5 ±0.5 ±0.7 ± ±1.0 ±1 ±0.3 ± ±0.6 ±1.2 ±0.2 ±0.4 1,000μL 100-1,000μL 100 ±3.0 ±3 ±0.6 ± ±1.0 ±5 ±0.2 ±1 1,000 ±0.6 ±6 ±0.2 ±2 5,000μL 500-5,000μL 500 ±2.4 ±12 ±0.6 ±3 2,500 ±1.2 ±30 ±0.25 ±6.25 5,000 ±0.6 ±30 ±0.15 ±7.5 5,000μL 1-10mL 1,000 ±3.0 ±30 ±0.6 ±6 5,000 ±0.8 ±40 ±0.2 ±10 10,000 ±0.6 ±60 ±0.15 ±15 29

30 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 20μL μL 1 ±2.5 ±0.025 ±1.8 ± ±1.5 ±0.075 ±0.8 ± ±1.0 ±0.1 ±0.4 ± μL 5-100μL 10 ±2.0 ±0.2 ±1.0 ± ±1.0 ±0.5 ±0.3 ± ±0.8 ±0.8 ±0.2 ± μL μL 30 ±2.5 ±0.75 ±0.7 ± ±1.0 ±1.5 ±0.3 ± ±0.6 ±1.8 ±0.2 ±0.6 1,000μL 50-1,000μL 100 ±3.0 ±3 ±0.6 ± ±1.0 ±5 ±0.2 ±1 1,000 ±0.6 ±6 ±0.2 ±2 5,000μL 100-5,000μL 500 ±3.0 ±15 ±0.6 ±3 2,500 ±1.2 ±30 ±0.25 ±6.25 5,000 ±0.6 ±30 ±0.15 ±7.5 30

31 V 3120 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 10μL μL 0.1 ±48.0 ±0.048 ±12 ± ±12.0 ±0.03 ±6.0 ± ±2.5 ±0.031 ±1.5 ± ±1.4 ±0.035 ±0.7 ± μL 20μL 20μL, L μL 0.5 ±8.0 ±0.04 ±5.0 ± ±2.5 ±0.025 ±1.8 ± ±1.5 ±0.075 ±0.8 ± ±1.0 ±0.1 ±0.4 ± μL 20μL, L 2-20μL 2 ±5.0 ±0.1 ±1.5 ± ±1.2 ±0.12 ±0.6 ± ±1.0 ±0.2 ±0.3 ± μL 2-20μL 2 ±5.0 ±0.1 ±1.5 ± ±1.2 ±0.12 ±0.6 ± ±1.0 ±0.2 ±0.3 ± μL 10 ±3.0 ±0.3 ±1.0 ± ±1.0 ±0.5 ±0.3 ± ±0.8 ±0.8 ±0.2 ± μL 20 ±2.5 ±0.5 ±0.7 ± ±1.0 ±1.0 ±0.3 ± ±0.6 ±1.2 ±0.2 ± μL μL 30 ±2.5 ±0.75 ±0.7 ± ±1.0 ±1.5 ±0.3 ± ±0.6 ±1.8 ±0.2 ±0.6 1,000μL 100-1,000μL 100 ±3.0 ±3 ±0.6 ± ±1.0 ±5 ±0.2 ±1 1,000 ±0.6 ±6 ±0.2 ±2 31

32 9 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 5,000μL 500-5,000μL 500 ±2.4 ±12 ±0.6 ±3 2,500 ±1.2 ±30 ±0.25 ±6.25 5,000 ±0.6 ±30 ±0.15 ±7.5 10mL 1-10mL 1,000 ±3.0 ±30 ±0.6 ±6 5,000 ±0.8 ±40 ±0.2 ±10 10,000 ±0.6 ±60 ±0.15 ± Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 2 ±6.0 ±0.12 ±4.0 ± ±5.0 ±0.15 ±3.0 ± ±4.0 ±0.2 ±2.0 ± ±3.0 ±0.3 ±1.5 ± ±2.0 ±0.4 ±0.8 ± Systematic error (inaccuracy) Random error (imprecision) ml % ml % ml S 2.5 ±1.0 ±0.025 ±0.2 ± ±0.4 ±0.02 ±0.2 ± ±0.3 ±0.03 ±0.2 ± P 1 ±0.6 ±0.006 ±0.2 ± ±0.5 ±0.025 ±0.1 ± ±0.3 ±0.03 ±0.1 ±

33 Xplorer Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 10μL 20μL 20μL, L μL 1 ±2.5 ±0.025 ±1.8 ± ±1.5 ±0.075 ±0.8 ± ±1.0 ±0.1 ±0.4 ± μL 5-100μL 10 ±2.0 ±0.2 ±1.0 ± ±1.0 ±0.5 ±0.3 ± ±0.8 ±0.8 ±0.2 ± μL μL 30 ±2.5 ±0.75 ±0.7 ± ±1.0 ±1.5 ±0.3 ± ±0.6 ±1.8 ±0.2 ±0.6 1,000μL 50-1,000μL 100 ±3.0 ±3 ±0.6 ± ±1.0 ±5 ±0.2 ±1 1,000 ±0.6 ±6 ±0.2 ±2 5,000μL 250-5,000μL 500 ±3.0 ±15 ±0.6 ±3 2,500 ±1.2 ±30 ±0.25 ±6.25 5,000 ±0.6 ±30 ±0.15 ±7.5 10mL mL 500 ±3.0 ±30 ±0.6 ±6 2,500 ±0.8 ±40 ±0.2 ±10 5,000 ±0.6 ±60 ±0.15 ±15 33

34 M 3114 μl μl Systematic error (inaccuracy) Random error (imprecision) % μl % μl 10μL 20μL 20μL, L μL 8 1 ±8.0 ±0.08 ±5.0 ± ±4.0 ±0.2 ±2.0 ± ±2.0 ±0.2 ±1.0 ± μL μL 8 10 ±3.0 ±0.3 ±2.0 ± ±1.0 ±0.5 ±0.8 ± ±0.8 ±0.8 ±0.3 ± μL μL 8 30 ±3.0 ±0.9 ±1.0 ± ±1.0 ±1.5 ±0.5 ± ±0.6 ±1.8 ±0.3 ±

35 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 10μL 20μL 20μL, L μL 1 ±5.0 ±0.05 ±3.0 ± ±3.0 ±0.15 ±1.5 ± ±2.0 ±0.2 ±0.8 ± μL 5-100μL 10 ±2.0 ±0.2 ±2.0 ± ±1.0 ±0.5 ±0.8 ± ±0.8 ±0.8 ±0.25 ± μL μL 30 ±2.5 ±0.75 ±1.0 ± ±1.0 ±1.5 ±0.5 ± ±0.6 ±1.8 ±0.25 ±0.75 1,250μL 50-1,200μL 120 ±6.0 ±7.2 ±0.9 ± ±2.7 ±16.2 ±0.4 ±2.4 1,200 ±1.2 ±14.4 ±0.3 ± M 3122 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 10μL 20μL 20μL, L μL 0.5 ±12.0 ±0.06 ±8.0 ± ±8.0 ±0.08 ±5.0 ± ±4.0 ±0.2 ±2.0 ± ±2.0 ±0.2 ±1.0 ± μL μL 10 ±3.0 ±0.3 ±2.0 ± ±1.0 ±0.5 ±0.8 ± ±0.8 ±0.8 ±0.3 ± μL μL 30 ±3.0 ±0.9 ±1.0 ± ±1.0 ±1.5 ±0.5 ± ±0.6 ±1.8 ±0.3 ±0.9 35

36 Xplorer Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 10μL 20μL 20μL, L μL 1 ±5.0 ±0.05 ±3.0 ± ±3.0 ±0.15 ±1.5 ± ±2.0 ±0.2 ±0.8 ± μL 5-100μL 10 ±2.0 ±0.2 ±2.0 ± ±1.0 ±0.5 ±0.8 ± ±0.8 ±0.8 ±0.25 ± μL μL 30 ±2.5 ±0.75 ±1.0 ± ±1.0 ±1.5 ±0.5 ± ±0.6 ±1.8 ±0.25 ±0.75 1,250μL 50-1,200μL 120 ±6.0 ±7.2 ±0.9 ± ±2.7 ±16.2 ±0.4 ±2.4 1,200 ±1.2 ±14.4 ±0.3 ±3.6 36

37 ~25 C ±0.5 C 10 ISO 8655 stream/xstream DIS speed level ml μl Systematic error (inaccuracy) Random error (imprecision) % μl % μl ±1.6 ±0.032 ±3.0 ± ±1.0 ±0.2 ±2.0 ± ±1.3 ±0.052 ±2.0 ± ±0.8 ±0.32 ±1.5 ± ±0.9 ±0.09 ±1.5 ± ±0.8 ±0.8 ±0.6 ± ±0.9 ±0.18 ±0.9 ± ±0.6 ±1.2 ±0.4 ± ±0.8 ±0.4 ±0.8 ± ±0.5 ±2.5 ±0.3 ± ±0.6 ±0.6 ±0.6 ±0.6 1,000 ±0.5 ±5 ±0.25 ± ±0.5 ±1 ±0.6 ±1.2 2,000 ±0.5 ±10 ±0.25 ± ±0.4 ±2 ±0.6 ±3 5,000 ±0.3 ±15 ±0.25 ± ,000 ±0.3 ±3 ±0.5 ±5 10,000 ±0.3 ±30 ±0.3 ±30 37

38 stream/xstream ml μl Systematic error (inaccuracy) Random error (imprecision) % μl % μl ±1.6 ±0.16 ±2.5 ± ±1.0 ±0.5 ±1.5 ± ±1.0 ±1 ±0.5 ± ±1.3 ±0.26 ±1.5 ± ±1.0 ±1 ±1.0 ±1 200 ±1.0 ±2 ±0.5 ± ±0.9 ±0.45 ±0.8 ± ±0.9 ±2.25 ±0.5 ± ±0.9 ±4.5 ±0.3 ± ±0.9 ±0.9 ±0.55 ± ±0.6 ±3 ±0.3 ± ±0.6 ±6 ±0.2 ± ±0.8 ±2 ±0.45 ± ,250 ±0.5 ±6.25 ±0.3 ±3.75 2,500 ±0.5 ±12.5 ±0.15 ± ±0.8 ±4 ±0.35 ±1.75 2,500 ±0.5 ±12.5 ±0.25 ±6.25 5,000 ±0.5 ±25 ±0.15 ± ,000 ±0.5 ±5 ±0.25 ±3 5,000 ±0.4 ±20 ±0.25 ±13 10,000 ±0.4 ±40 ±0.15 ± ,500 ±0.3 ±8 ±0.35 ±8.8 12,500 ±0.3 ±38 ±0.25 ±31 25,000 ±0.3 ±75 ±0.15 ± ,000 ±0.3 ±15 ±0.50 ±25 25,000 ±0.3 ±75 ±0.20 ±50 50,000 ±0.3 ±150 ±0.15 ±75 38

39 ~25 C ±0.5 C 10 ISO Systematic error (inaccuracy) Random error (imprecision) mL 2.5mL ±0.6% 0.1% mL 5.0mL ±0.5% 0.1% 2-10mL 10mL ±0.5% 0.1% 5-25mL 25mL ±0.5% 0.1% 10-50mL 50mL ±0.5% 0.1% mL 100mL ±0.5% 0.1% Systematic error (inaccuracy) Random error (imprecision) M 25mL ±0.2% 0.1% H 50mL ±0.2% 0.1% 39

40 9 9.6 EN ISO 8655 μl Error limit for systematic error (inaccuracy) 1μL 2μL 5μL 10μL 20μL 50μL 100μL 200μL 500μL 1,000μL 2,000μL 5,000μL 10,000μL ±5.0% ±4.0% ±2.5% ±1.2% ±1.0% ±1.0% ±0.8% ±0.8% ±0.8% ±0.8% ±0.8% ±0.8% ±0.6% ±0.05μL ±0.08μL ±0.125μL ±0.12μL ±0.2μL ±0.5μL ±0.8μL ±1.6μL ±4.0μL ±8.0μL ±16.0μL ±40.0μL ±60.0μL Error limit for random errors (imprecision) ±5.0% ±0.05μL ±2.0% ±0.04μL ±1.5% ±0.075μL ±0.8% ±0.08μL ±0.5% ±0.1μL ±0.4% ±0.2μL ±0.3% ±0.3μL ±0.3% ±0.6μL ±0.3% ±1.5μL ±0.3% ±3.0μL ±0.3% ±6.0μL ±0.3% ±15.0μL ±0.3% ±30.0μL 2 40

41 Error limit for systematic error (inaccuracy) Error limit for random errors (imprecision) 5μL ±2.5% ±0.13μL ±1.5% ±0.08μL 10μL ±2.0% ±0.2μL ±1.0% ±0.1μL 20μL ±2.0% ±0.4μL ±0.8% ±0.16μL 50μL ±1.4% ±0.7μL ±0.6% ±0.3μL 100μL ±1.5% ±1.5μL ±0.6% ±0.6μL 200μL ±1.5% ±3.0μL ±0.4% ±0.8μL 500μL ±1.2% ±6.0μL ±0.4% ±2.0μL 1,000μL ±1.2% ±12.0μL ±0.4% ±4.0μL Error limit for systematic error (inaccuracy) Error limit for random errors (imprecision) 0.001mL ±5.0% ±0.05μL ±5.0% ±0.05μL 0.002mL ±5.0% ±0.1μL ±5.0% ±0.1μL 0.003mL ±2.5% ±0.075μL ±3.5% ±0.11μL 0.01mL ±2.0% ±0.2μL ±2.5% ±0.25μL 0.02mL ±1.5% ±0.3μL ±2.0% ±0.4μL 0.05mL ±1.0% ±0.5μL ±1.5% ±0.75μL 0.1mL ±1.0% ±1.0μL ±1.0% ±1.0μL 0.2mL ±1.0% ±2.0μL ±1.0% ±2.0μL 0.5mL ±1.0% ±5.0μL ±0.6% ±3.0μL 1mL ±1.0% ±10.0μL ±0.4% ±4.0μL 2mL ±0.8% ±16.0μL ±0.4% ±8.0μL 5mL ±0.6% ±30.0μL ±0.3% ±15.0μL 10mL ±0.5% ±50.0μL ±0.3% ±30.0μL 25mL ±0.5% ±125.0μL ±0.3% ±75.0μL 50mL ±0.5% ±250μL ±0.25% ±125.0μL 100mL ±0.5% ±500μL ±0.25% ±250.0μL 200mL ±0.5% ±1,000μL ±0.25% ±500.0μL 41

42 Error limit for systematic error (inaccuracy) Error limit for random errors (imprecision) 0.01mL ±2.0% ±0.2μL ±1.0% ±0.1μL 0.02mL ±2.0% ±0.4μL ±0.5% ±0.1μL 0.05mL ±1.5% ±0.75μL ±0.4% ±0.2μL 0.1mL ±1.5% ±1.5μL ±0.3% ±0.3μL 0.2mL ±1.0% ±2.0μL ±0.3% ±0.6μL 0.5mL ±1.0% ±5.0μL ±0.2% ±1.0μL 1mL ±0.6% ±6.0μL ±0.2% ±2.0μL 2mL ±0.6% ±12.0μL ±0.2% ±4.0μL 5mL ±0.6% ±30.0μL ±0.2% ±10.0μL 10mL ±0.6% ±60.0μL ±0.2% ±20.0μL 25mL ±0.6% ±150.0μL ±0.2% ±50.0μL 50mL ±0.6% ±300.0μL ±0.2% ±100.0μL 100mL ±0.6% ±600.0μL ±0.2% ±200.0μL 200mL ±0.6% ±1,200μL ±0.2% ±400.0μL Error limit for systematic error (inaccuracy) Error limit for random errors (imprecision) 1mL ±0.6% ±6.0μL ±0.1% ±1.0μL 2mL ±0.5% ±10.0μL ±0.1% ±2.0μL 5mL ±0.3% ±15.0μL ±0.1% ±5.0μL 10mL ±0.3% ±30.0μL ±0.1% ±10.0μL 10mL ±0.2% ±40.0μL ±0.1% ±20.0μL 25mL ±0.2% ±50.0μL ±0.1% ±25.0μL 50mL ±0.2% ±100.0μL ±0.1% ±50.0μL 100mL ±0.2% ±200μL ±0.1% ±100.0μL 42

43

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