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1 3D PCR 3D PCR PCR 2, 3D PCR PCR 3D PCR PCR PCR PCR 1 PCR PCR Ct CNV mrna mirna GMO

2 サンプル調製サンプルの分配 DNA の増幅結果 コピー数 / µl サンプルを多数の反応に分配します ポジティブ反応ネガティブ反応 1. PCRPCR 1 PCR PCR Ct 3D PCR PCR 3D PCR 1 /µl 2 3D PCR 2, 31 PCR TaqMan PCR TaqMan PCR Life Technologies 8 TaqMan 3D PCR 3D PCR 1 2 PCR 2. PCR 3D PCR

3 3. 3D PCR 2K 2, 1 1/ 1 3D PCR copies/µl 4 2 AnalysisSuite 2 Life Technologies USB QC 3D AnalysisSuite D PCR1/µL Life Technologies 3D AnalysisSuite 3D AnalysisSuite * * 3D PCR

4 PCR 数 数 2, 3D PCR 5 6 ー CNV ー A B コピー数 /µl 2 19 コピー数 /µl HPRT1 MYC VEGFA 18S B2M GAPDH GUSB PP1A AssayID AssayID 5. 3D PCR TaqMan Gene Expression 85 1% A HPRT1 MYC VEGFA B 18S B2M GAPDH GUSB PPIA 15 1 コピー数 /ul 5 OA QS3D1 QS3D2 QS3D3 QS3D4 QS3D5 Life Vendor OA QS3D1 QS3D2 QS3D3 QS3D4 QS3D5 Life Vendor QS3D1 dpcr UV dpcr UV dpcr QS3D2 反復数 検出法 15 3 NTC 予想されるコピー数 /ul 6. 3D PCR 3D PCR 2 DNA Promega UV PCR 2 コピー数 /µl UV Promega VendorLife Technologies PCR 12K Flex OpenArray PCR ー OA 5 3D PCR QS3D ー ー 2 3 コピー /µl 1,5 コピー /µl ー 3D PCR

5 3D Coriell repository9 DNA 17 CCL3L1 TaqMan copy Number68 8 7A78 PCR 1.2 7B A CV % NA N/A NA NA NA NA NA NA NA NA B コピー数 NA17245 NA17251 NA17258 NA17132 NA19194 NA1857 NA1711 NA1722 NA18854 Samples CCL3L1 8 9DNA A PCR CV 2.55% B A PCR 78

6 PCR PCR 2, 3D PCR 2K 5log PCR TaqMan SNP Genotyping PCR 1 PCR PCR D PCR GMOCNV 3D PCR A 検出されたコピー数 /µl 9, 8, 7, 6, 5, 4, 3, 2, 1, 予想されるコピー数 /µl R 2 = , 2, 3, 4, 5, 6, 7, 8, B 検出されたコピー数 /µl 予想されるコピー数 /µl R 2 = D PCR 3 検出 ADNA UV 7, copies/µl2.5 copies/ µl 3D PCRB

7 3D PCR 3D PCR PCR H x W x D 3D PCR 2KQS3D Chips 1 LED CMOS FAM VIC ROX * 21 x 13.5 x cm 2.4 kg , 14.5 µl Yes 95% 5 logs 1% *ROX

8 Ordering information PCR 3D PCR 1 QS3D-GA 3D PCR GeneAmp PCR97 3D ChipLoader 2 PCR QS3D Chips Immersion fluid 3 QS3D Lids and Blades QS3D UV Sealing Kit UV 1 QS3D Digital PCR Master Mix 1.5 ml QS3D Digital PCR Master Mix, 5mL 5 ml PCR QS3D Chip Adapter Kit for GeneAmp PCR System QS3D Tilt Base for GeneAmp PCR System QS3D UV Pen Kit UV Pen 1UV Pen 1QS3D UV Sealing Kit 1 USB Wi-Fi Card QS3D AnalysisSuite 公式 Facebook ページ & Twitter もチェック! 取扱店 研究用にのみ使用できます 診断目的およびその手続き上での使用は出来ません 記載の社名および製品名は 弊社または各社の商標または登録商標です 標準販売条件はこちらをご覧ください 最新の価格および在庫数は 弊社ホームページ右上の 製品価格と在庫の検索 でご確認いただけます For Research Use Only. Not for use in diagnostic procedures. 214 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. Printed in Japan. SDS91-A143OB ライフテクノロジーズジャパン株式会社 本社 : 東京都港区芝浦 大阪 : 大阪府吹田市広芝町 1-28 TEL.3(6832)93 FAX. 3(6832)958 TEL.6(6389)121 FAX. 6(6389)126

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