Sourcebook_本文_ob_0706.indd
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- ゆきさ なみこし
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1 Primary Cell Culture Sourcebook
2 Welcome! GIBCO 3 in vivo 3 GIBCO 3D
3 D Neon Transfection System
4
5 (BPE) FREE A N O R I M A L I G I N (HIV-1 B C ) 70% 500,000cells (HEKn) HKGS S7 EpiLife Medium 30(HEKa) HKGS S7 EpiLife Medium25 BPE BPE HEKn-APFHEKa-APF EpiLife Medium S7 / Synth-a-Freeze HEKnHEKa EpiLife Medium EDGS /EDTA / Synth-a-Freeze HEKnHEKa EpiLife Medium HKGS /EDTA / Synth-a-Freeze HEKnHEKa Medium 154 HKGS /EDTA / Synth-a-Freeze 1
6 (HEK) A N O R I M A L FREE I G I N HEKn-APF ( R-011-K 15) EpiLife A N O R I M A L FREE I G I N (15 ) EpiLife in vitro 60 M 1 ( >500,000cells) C-020-5C M-EPI-500-CA HEKn 1 ( >500,000cells) C-001-5C (HEK) HEKa-APF ( R-011-K 15) 1 ( >500,000cells) C-021-5C A N O I M A L FREE R I G I N FREE EpiLife CF() (3 ) M-EPIcf-500 Medium 154CF Medium 154CF/PRF 0.5 M EpiLife CF/PRF( FREE ) EpiLife CF/PRF EpiLife (3 ) A N O R I M A L I G I N A N O R I M A L I G I N M-EPIcf/PRF-500 HEKa 1 ( >500,000cells) C-005-5C Medium 154 HKGS( S-001-5) HKGS( S-001-K) 200 M 2 M
7 Medium 154CF() Medium 154CF Medium 154 HKGS( S-001-5)HKGS( S-001-K) M-154CF-500 Medium 154CF/PRF( ) Medium 154CF/PRF Medium 154 HKGS( S-001-5) HKGS( S-001-K) M-154CF/PRF-500 EpiLife CF CF/PRF 0.65 M (HKGS) EpiLife Medium Medium 154 (100 ) (BPE)* 5ml S EpiLife MediumMedium 154 *BPE (HKGS) EpiLife Medium Medium 154HKGS HKGS (BPE)* / B (GA) GA 1 S-001-K EpiLife MediumMedium 154 EpiLife +HKGS EpiLife (EDGS) EpiLife Medium (100 ) (Medium 154 ) BSA( >95% Medium 154+HKGS HEKn 1 HEKn 3 HEKn 5 EpiLife Medium 154HEKn( C-001-5C) <0.005%FA) ( >95%) rhigf-1( >95%) rhegf( >95%) ( >96%) PGE-2( >98%) EDGS ( R-011-K 15) 5ml S
8 FREE S7 EpiLife Medium (100 ) S7 S7 5ml EpiLife Medium 154 S7 ( R-011-K 15) A N O R I M A L 5ml EpiLife Medium I G I N S mM(10 0.5ml) S-013-EPI mM(10 0.5ml) S (15) A N O R I M A L FREE I G I N (HIV-1 B C ) 70% 500,000cells HEMn-MP HEMn-DP12 HEMn-LP HEMa-LP16 4 HEMa-LP Mel-5 (HEM) HEMn-LP 1 ( >500,000cells) C-002-5C HEMn-MP 1 ( >500,000cells) C-102-5C HEMn-DP 1 ( >500,000cells) C-202-5C (HEM) HEMa-LP -2( S-016-5) 1 ( >500,000cells) C-024-5C 4
9 Medium 254 HMGS( S-002-5)HMGS-2( S-016-5) M Medium 254CF() Medium 254CF * Medium 254 HMGS( S-002-5)HMGS-2( S-016-5) M-254CF-500 * Medium 254CF 0.5 M (HMGS) Medium 254 Medium 254CF (100 ) (BPE) ml S BPE -2(HMGS-2) Medium 254 Medium 254CF (100 ) (BPE) -1 HEMa-LP 5ml S (15) BPE HEMa 6 Mel-5 HEMa 6 5
10 (HIV-1 B C ) 70% 500,000cells HDFa12 HDFn 16 (HDF) HDFn 1 ( >500,000cells) C-004-5C (HDF) HDFa 1 ( >500,000cells) C-013-5C 6
11 Medium 106 LSGS( S )LSGS( S-003-K) M (LSGS) LSGS LSGS / (BSA ) / B (GA) GA 1 S-003-K (15) (LSGS) Medium 106 (50 ) 10ml S HDFn 3 HDFn 5 HDFa 5 HDFa D 7
12 (HIV-1 B C ) 70% 500,000cells 16 dii-ac-ldl von Willebrand (vwf) CD31CD36( ) - ( ) dii-ac-ldl -vwf -CD36 -CD31 (HMVEC) HMVECnd HMVECad 1 HMVECad 3 1 ( >500,000cells) C-010-5C (HMVEC) HMVECad HMVECad 5 1 ( >500,000cells) C-011-5C 8
13 Medium 131+ MVGS( S ) 1 (100ml S ) (MVGS) Medium 131 (20 ) dbcamp M ml S (15) (AF) (1X) AF (HIV-1 B C ) 70% 16 dii-ac-ldl von Willebrand (vwf) CD31( ) - ( ) 100ml S (15) HAEC 1 ( >500,000cells) C-006-5C HPAEC 1 ( >500,000cells) C-008-5C 9
14 HUVEC 1 ( >500,000cells) C-003-5C HUVECp Medium 200 LSGS( S )LSGS( S-003-K) M >500,000 / 1 C-015-5C ( >500,000cells) >1,000,000 / 1 C C ( >1,000,000cells) Medium 200PRF() Medium 200PRFMedium 200 LSGS( S ) LSGS( S-003-K) M-200PRF-500 HUVEC 3 HUVEC 5 HUVEC 7 HUVEC -CD31 HUVEC dii-ac-ldl HUVEC -vwf 10
15 11 (LSGS) Medium 200 Medium 200PRF (50 ) 10ml S (LSGS) LSGS LSGS / (BSA ) / B (GA) GA 1 S-003-K (15) (HIV-1 B C ) 70% 500,000cells16 SMDS- ( )von Willebrand (vwf ) HASMC 1 ( >500,000cells) C-007-5C HCASMC 1 ( >500,000cells) C-017-5C HPASMC 1 ( >500,000cells) C-009-5C 3D HASMC 5 HPASMC 5 HCASMC 5
16 Medium 231 SMGS( S ) SMDS( S-008-5) M / (SMGS) Medium 231 (20 ) BSA 25ml S (SMDS) Medium 231 (100 ) 5ml S (15) 12
17 13 EpiLife Medium HCGS( S-009-5) 60 M M-EPI-500-CA EpiLife CF() * HCGS( S-009-5) M-EPIcf-500 HCEC-2 5 HCEC-2 9 HCEC-2 7 HCEC-3 5 HCEC-3 9 HCEC-3 7 (HIV-1 B C ) 70% 500,000cells (HCEC) HCEC-2 1 ( >500,000cells) C-018-5C EpiLife CF/PRF( ) EpiLife CF/PRF * EpiLife HCGS( S-009-5) M-EPIcf/PRF-500 * EpiLife CFEpiLife CF/PRF 0.65 M (HCGS) EpiLife (100 ) (BPE) () 5ml S (15) BPE
18 5/6 8 18E- 26 HMEC 1 ( >500,000cells) A10565 (MEGS) Medium 171 (100 ) (BPE)* 5ml S *BPE Medium 171 MEGS( S-015-5) M MEGM M171/MEGS Medium 171/MEGS2 (MEGS) Medium 171 (HMEC) HMEC (8 )9 14
19 /EDTA (TE) EDTA (1X) 100ml R Synth-a-Freeze 10% DMSO 50ml A A N O I M A L FREE R I G I N (TN) (1X) 10 A N O I M A L FREE R I G I N 100ml R mM ml S-013-EPI mM ml S (DTI) (1X) 100ml A N O R I M A L FREE I G I N R / B FREE (GA) 10 A N O R I M A L I G I N B (500X) (10 1ml / ) GA ml R T-25 1 R-011-K A N O R I M A L FREE I G I N 15
20 Clostridium histolyticum (Clostridiopeptidase A) E. C /mg L- 1 mol Bacillus polymyxa 5g mg Fungizone TM 1ml250 g B Clostridium histolyticum 1g g/ml Fungizone TM E.R. Squibb & Sons, L.L.C. 20ml Clostridium histolyticum 1g Clostridium histolyticum 50mg/ml g/ml 10mg/ml 10ml ml() mg/ml 10ml ml() g
21 Recovery TM Recovery TM Recovery TM 25% - -DMSO DulbeccoEagle Medium(DMEM)10% DMSO 50ml Recovery TM Medium AE-1 CHO MDCK Swiss 3T3 HeLa Recovery TM CCFM S S SC C SM GM BL / / (AE-1 2 CHO 6 MDCK 6 Swiss 3T3 5 HeLa 6 ) Recovery TM (CCFM)(n=4) TrypLE TM Select(1X) 1mM EDTAD-PBS ml
22 TrypLE TM Express(1X) 0.6 TrypLE TM Express TrypLE TM Select AU mM EDTAD-PBS ml ml() TrypLE TM Express() (1X) 1mM EDTAD-PBS AU TrypLE ml ml() TrypLE TM 0.25%(1X) Hank2.5g/L (1:250) (MgCl 2 6H 2 O) (MgSO 4 7H 2 O) TrypLE 100ml TrypLE TM TrypLE TM Express TrypLE TM Express 1 HPLC 2 TrypLE TM Express 18
23 2.5%(10X) 25g/L(1:250) 8.5g/L 1X 2.5g(1:250)/L -EDTA (0.5%EDTA 4Na)(10X) 5.0g/L(1:250) 2.0g/L EDTA 4Na 8.5g/L 1X 0.5g0.2g EDTA 4Na/L(1:250) 100ml ml EDTA (0.05%EDTA 4Na)(1X) Hank 0.5g/L(1:250) 0.2g/L EDTA 4Na (MgCl 2 6H 2 O) (MgSO 4 7H 2 O) -EDTA (0.25%EDTA 4Na)(1X) Hank 2.5g/L(1:250) 0.38g/L EDTA 4Na (MgCl 2 6H 2 O) (MgSO 4 7H 2 O) 100ml ml() ml ml()
24 GIBCO (SFM) Serum-free media-gibco Protein-free media-gibco Chemically difined media-gibco Animal origin-free media-gibco ( ) Neurobasal Medium Neurobasal Medium Neurobasal -A Medium Neurobasal -A Medium (1 ) B-27 AO 10ml B-27 AO B-27 (AOB-27 10ml B-27 A( ) ) CNS 10ml N-2 G-5 (PC12, B104, N1E- 115NS20) (U-251, MGsp, C62BD, RN-22) ( <125 g/ ml) 5ml ml
25 AIM V Medium* HSA AIM V Medium IL-2 ex vivo (TIL ) T (GM-CSF ) StemPro -34 (StemPro ) * (CD34+) ( )/ 1,000ml 1,000ml 10L DK BK BG01V/hOG hesc hoct4-gfp StemPro hesc StemPro EZPassage KnockOut (KSR) (GFP) Oct-4 BG01V feeder (ESC) FBS hescs ( ) BG01 BG02 BG03 H1 H9 BG01V HUES6 HUES9 CyT49 Cyth203 ES (R366.4) ESCESC / ESC in vitro KSR FBS KnockOut DMEM KnockOut DMEM/F-12 ES ( ) ES (USDA ) StemPro MesenPRO RS Medium MSC (USDA ) ADSC- 1 (2%) ADSC 1 ~ R * 1 A ml 100ml R ml
26 bfgf EGF ( ) (EGF BPE )* ( )* CD ( ) ( ) (9 P450) in vitro BPE (<25 g/ml) HuMEC HuMEC HuMEC ml( )25mg KnockOut D-MEM (ES) ES KnockOut ES LHC 1,000ml LHC LHC-8 ( ) LHC CD- 1ml * 22
27 3D in vivo in vivo Geltrex TM (LDEV ) Geltrex TM (RGF) (BME) Engelbreth-Holm-Swarm(EHS) entactin (3D) Geltrex TM Geltrex TM Geltrex TM LDEV(Lactete Dehydrogenase-Elevating Virus: ) in vivo 3D 3D 10 g/ml DulbeccoEagle LDEV SVEC4-105%FBS Geltrex TM Geltrex TM Geltrex TM 1 ml A * 5 ml A * 1 ml A A * 5 ml A A * * LDEV 23
28 GIBCO AlgiMatrix TM 3D AlgiMatrix TM 3D MCTS 3 / 10 6 / D 2% AlgiMatrix TM Algimatrix TM Firming Buffer ( A ) 6wells Kit MC 3-MC 3-MC 3-MC Furafylline 24 Furafylline 48 1 C3A CYP1A2( P450)3-MC() CYP1A2 2 A B AlgiMatrix TM 3D(MCTS) D 7 in vitro C D 1. Kunz-Schughart, L., et al., (2004) J Biomolec Screening 9, Dvir-Ginzberg, M., et al. (2003) Tissue Engineering 9, Elkayam, T., et al. (2006) Tissue Engineering 12, Kedem, A., et al. (2005) Tissue Engineering 11, Dar, A., et al. (2002) Biotechnology & Bioengineering 80, Kim, J. (2005) Seminars in Cancer Biology 15, Gerecht-Nir, S., et al. (2004) Biotechnology & Bioengineering 88, AlgiMatrix TM A. B. 8- ( )propidium iodide C. ( ) D. PCNA ( ) AlgiMatrix TM 3D Culture System in 96-wells plate 96 / AlgiMatrix TM 3D Culture System in 24-wells plate 24 / AlgiMatrix TM 3D Culture System in 96-wells plate 96 / AlgiMatrix TM 3D Culture System in 24-wells plate 24 / AlgiMatrix TM 3D Firming Buffer 50 ml A AlgiMatrix TM 3D Culture System in 6-wells plate and Buffer 1kit (6 /1) A AlgiMatrix TM 3D Culture System in 6-wells plate and Buffer 1kit (6 /4) A
29 DNA RNA BT Lipofectamine TM LTX PLUS TM Lipofectamine TM LTX 90% Lipofectamine TM LTXPLUS TM (HUVEC) HUVEC (Matrigel) 1 2 Lipofectamine TM LTX HUVEC (>50%) Matrigel Lipofectamine TM LTXPLUS TM 1. Bernardini et al. (2000) I-309 binds to and activates endothelial cell functions and acts as an angiogenic molecule in vivo. Blood 96, Mukherjee et al. (2005) Cytotoxic and anti-angiogenic activity of AW464 (NSC ), a novel thioredoxin inhibitor: An in vitro study. British Journal of Cancer 92, Lipofectamine LTX 1.0ml PLUS 0.85ml ng 3D 5% 10% Lipofectamine TM LTXPLUS TM 50% 500ng 1,000ng Lipofectamine TM LTXPLUS TM PLUS TM (2.5 l) Lipofectamine TM LTX (1.5 l) GFP- 24 GFP Beverly Davidson 25
30 Neon Transfection System DNAsiRNA 80% Neon Transfection Neon Transfection 24 Protocol Exchange GIBCO Neon Transfection Neon Transfection System 1 1 MPK5000 Neon Transfection System Starter Pack 2 1 MPK5000S Neon Transfection System Kit (100 µl) 1 MPK10096 Neon Transfection System Kit (10 µl) 1 MPK1096 Neon Transfection Tubes (100 ) 1 MPT100 Neon Pipette MPP100 Neon Pipette Station MPS100 1 Neon Transfection System(MPK5000) Neon Neon Pipette(MPP100) Neon Pipette Station(MPS100) 2 Starter Pack(MPK5000S)Neon Transfection System (MPK 5000) Transfection Kit(MPK10096 MPK ) OD SCFM07e ED 50 (pg/ml) BioSource = = 6, SCF (pg/ml) (SCF) M07e SCF SCF (ED 50 ) 26
31 Human proteins Recombinant human Activin A 5 µg PHG9014 Recombinant human BDNF 10 µg PHC7074 Recombinant human BMP-4 10 µg PHC7914 Recombinant human EGF 200 µg PHG0064 Recombinant human FGFb (truncated) 10 µg PHG0024 Recombinant human FGFb (full length) 10 µg PHG0264 Recombinant human Flt-3 ligand 10 µg PHC1704 Recombinant human EGF 100 µg PHG0311 Recombinant human GDNF 10 µg PHC7045 Recombinant human GM-CSF 10 µg PHC2015 Recombinant human HGF 10 µg PHG0254 Recombinant human IFN-gamma 100 µg PHC4031 Recombinant human IGF µg PHG0075 Recombinant human IL-1 beta 10 µg PHC0815 Recombinant human IL-2 10 µg PHC0026 Recombinant human IL-3 10 µg PHC0034 Recombinant human IL-4 10 µg PHC0044 Recombinant human IL-6 10 µg PHC0065 Recombinant human IL-7 25 µg PHC0076 Recombinant human IL-8 25 µg PHC0084 Recombinant human IL µg PHC0214 Recombinant human IL µg PHC0334 Recombinant human M-CSF 10 µg PHC2044 Recombinant human Noggin 20 µg PHC1506 Recombinant human PDGF BB 10 µg PHG0045 Recombinant human SCF/cKit 10 µg PHC2115 Recombinant human SDF-1 a 10 µg PHC1344 Recombinant human TGF-beta 1 5 µg PHG9204 Recombinant human TNF-alpha 50 µg PHC3016 Mouse proteins Recombinant mouse Fgf basic 10 µg PMG0034 Recombinant mouse G-CSF 10 µg PMC2034 Recombinant mouse GM-CSF 10 µg PMC2015 Recombinant mouse IFN alpha 10 5 units PMC4016 Recombinant mouse IFN Beta 10 5 units PMC4024 Recombinant mouse IFN gamma 15 µg PMC4034 Recombinant mouse IL-3 10 µg PMC0034 Recombinant mouse IL-4 10 µg PMC0045 Recombinant mouse IL-6 10 µg PMC0065 Recombinant mouse IL µg PMC0126 Recombinant mouse IL µg PMC0334 Mouse Laminin (natural) 1 mg Recombinant mouse PDGF BB 10 µg PMG0044 Recombinant mouse SCF/cKit 10 µg PMC2114 Recombinant mouse Sonic Hedgehog 10 µg PMC2094 Recombinant mouse TNF alpha 10 µg PMC
32 Facebook! facebook.com/lifetechnologiesjapan TEL FAX TEL FAX FAX TEL FAX TEL FAX TEL FAX TEL FAX The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. 2012, Life Technologies Japan Ltd. All rights reserved. Printed in Japan. GIB027-B1207OB
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