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1) Zapf J, Walter H and Froesch ER: Radioimmunological determination of insulin-like growth factors I and II in normal subjects and in patients with growth disorders and extrapancreatic tumor

7 1) Zapf J, Walter H and Froesch ER: Radioimmunological determination of insulin-like growth factors I and II in normal subjects and in patients with growth disorders and extrapancreatic tumor hypoglycemia. J Clin Invest (1981) 68, ) Humbel RE: Insulin-like growth factors I and II. Eur J Biochem (1990) 190, ) Zapf J, Schonle E and Froesch ER: Insulin-like growth factors I and II: Some biological actions and receptor binding characteristics of two purified constituents of non-suppressible insulin-like activity of human serum. Eur J Biochem (1978) 87, ) Lindahl A, Isgaard J and Carlsson L: Differential effects of growth hormone and IGF-I on colony formation of epiphyseal chondrocytes in suspension culture in rats of different ages. Endocrinology (1987) 121, ) Ernst M and Rodan GA: Increased activity of insulin-like growth factor (IGF) in osteoblastic cells in the presence of growth hormone (GH): positive correlation with the presence of GH-induced IGF-binding protein-3. Endocrinology (1990) 127, ) Mohan S, Bautista CM, Wergedal JE and Baylink DJ: Isolation of an inhibitory insulin-like growth factor (IGF) binding protein from bone cell-conditioned medium: a potential local regulator of IGF action. Proc Natl Acad Sci USA (1989) 86, ) La Tour D, Mohan S, Linkhart TA, Baylink DJ and Strong DD: Inhibitory insulin-like growth factor-binding protein: Cloning, complete sequence, and physiological regulation. Mol Endocrinol (1990) 4, ) Kiefer MC, Masiarz FR, Bauer DM and Zapf J: Identification and molecular cloning of two new

30 kda insulin-like growth factor binding proteins isolated from adult human serum. J Biol Chem (1991) 266, 9043-9049.

8 30 kda insulin-like growth factor binding proteins isolated from adult human serum. J Biol Chem (1991) 266, ) Rosen C, Donahue LR, Hunter S, Holick M, Kavookjian H, Kirschenbaum A, Mohan S and Baylink DJ: The 24/25-kDa serum insulin-like growth factor-binding protein is increased in elderly women with hip and spine fractures. J Clin Endocrinol & Metab (1992) 74, ) Scharla SH, Strong DD, Rosen C, Mohan S, Holick M, Baylink and Linkhart TA: 1,25 - Dihydroxyvitamin D3 increases secretion of insulin-like growth factor binding protein-4 (IGFBP-4) by human osteoblast-like cells in vitro and elevates IGFBP-4 serum levels in vivo. J Clin Endocrinol & Metab (1993) 77, ) Shimasaki S, Gao L, Shimonaka M and Ling N: Isolation and molecular cloning of insulin-like growth factor-binding protein-6. Mol Endocrinol (1991) 5, ) Malpe S, Strong DD, Baylink DJ and Mohan S: Stimulation of human bone cell proliferation by an antisense oligodexyribonucleotide to IGFBP-4 mrna. J Bone Miner Res (1992) 7 (Suppl. 1), S ) Cheung PT, Smith EP, Shimasaki S, Ling N and Chernausek SD: Characterization of an insulin like growth factor binding protein (IGFBP-4) produced by B104 rat neuronal cell line: chemical and biological properties and differential synthesis by sublines. Endocrinology (1991) 129, ) Culouscou JM and Shoyab M: Purification of a colon cancer cell growth inhibitor and its identifica tion as an insulin-like growth factor binding protein. Cancer Res (1991) 51, ) Chen TL, Liu F, Bates RL and Hintz RL: Further characterization of insulin-like growth factor binding proteins in rat osteoblast-like cell culture: modulation by 17ƒÀ-estradiol and human growth hormone. Endocrinology (1991) 128, ) Greenberg C, Kukreja SC, Bowser EN, Hargis GK, Henderson WJ and Williams GA: Parathyroid hormone secretion: effect of estradiol and progesterone. Metabolism (1987) 36, ) Torring O, Firek AF, Heath HIII and Conover CA: Parathyroid hormone and parathyroid hormone related peptide stimulate insulin-like growth factor-binding protein secretion by rat osteoblast-like cells through a adenosine 3 L,5 L-monophosphate-dependent mechanism. Endocrinology (1991) 128, ) Mohan S and Baylink DJ: Evidence that the inhibition of TE85 human bone cell proliferation by agents which stimulate camp production may in part be mediated by changes in the IGF-II regulatory system. Growth Regulation (1991) 1, ) Lee K, Mohan S, Baylink DJ and Strong DD: Evidence for transcriptional regulation of insulin-like growth factor binding protein-4 (IGFBP-4) gene in human bone cells. J Bone Miner Res (1992) 7 (Supple. 1), ) Scharla SH, Strong DD, Mohan S, Baylink DJ and Linkhart TA: 1, 25-Dihydroxyvitamin D3 differentially regulates the production of insulin-like growth factor (IGF-I) and IGF-binding protein -4 in mouse osteoblast. Endocrinology (1991) 129, ) Mohan S, Strong DD, Lempert UG, Wergedal JE and Baylink DJ: Studies on regulation of insulin-like growth factor binding protein (IGFBP)-3 and IGFBP-4 production in human bone cells. Acta Endocrinol (1992) 127, ) Isaksson OGP, Lindahl A, Nilsson A and Isgaard J: Mechanism of the stimulatory effect of growth hormone on longitudinal bone growth. Endocr Rev (1987) 8,

9 Insulin-like growth factor binding protein-4 (IGFBP-4) and bone growth Yoshitaka in childhood YAMANAKA Department of Pediatrics, Okayama University Medical School, Okayama 700, Japan (Director: Prof. Y. Seino) IGFBP-4 is a 24kD protein, originally isolated from the conditioned medium of human bone cells, and has been implicated as a potent inhibitor of IGF action in vitro. To clarify its biological functions in human bone, I measured the serum concentration of IGFBP-4 from patients with endocrinologic disorders, using Western immunoblot. In normal children, IGFBP-4 increased with age. The serum IGFBP-4 levels of patients with GH deficiency and panhypopituitarism were lower than those of normal children. In addition, the serum levels of IGFBP-4 were also significantly lower in children with Turner's syndrome than in the age-matched normal children who were post pubertal. These findings suggest that GH and the sex steroid increase the production of serum IGFBP-4. Then, I examined the influence of GH on the serum level of of IGFBP-4. In the good responders to GH therapy, IGFBP-4 decreased rapidly during the first month, continued to decrease until the third month, and then increased slowly. On the contrary, in the poor responders to GH therapy, the IGFBP-4 level increased until the third month and then remained at a high level. The increased serum IGFBP-4 level may, at least in part, contribute to the low response to GH therapy in the poor responders.

日本消化器外科学会雑誌第29巻第9号

日本消化器外科学会雑誌第29巻第9号 Table 1 Oligonucleotide primers used for RT-PCR and internal probes used for Southern blot hybridization Cytokine Primer Sequence (5'-3') 5' 3' Internal probe s' 3' Internal probe 5' 3' Internal probe