[PDF] マニュアル AKTApurifier UNICORN 5.0
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- みさき あさま
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1 ÄKTApurifier UNICORN
2 UNICORN Ver.5.0 UNICORN Ver.5.0 PumpWash PurifierContinue PumpWash Pause Continue Frac-950 Frac Manual Flowpath OutletValve; F2 Execute Manual Frac Man_Fractionation Execute Frac-950 WizardElution Fractionation Start at First Tube Segment1 Fraction Volume Fraction Volume 0 First Tube Segment Segment1First Tube OFF10ON UV Cond/pH Frac-900 WizardElution Technique Gradient Segmented Gradient Advanced Segmented Gradient Advanced
3 Contents ÄKTApurifier ÄKTApurifier UNICORN UNICORN PumpWash Frac Frac Wizard Equilibration Elution Segment Segment Variables...40
4 7.11 Run Setup Copy to External UNICORN CD Copy from External Rename...66
5 PV-908 INV ÄKTApurifier On-line Help 1.1 ÄKTApurifier Box-900 INV-907 ph/c-900 UV-900 PV-908 M-925 P-903 M-925 (W1, W2 and W3) A SV-903 B SV-903 UV G1 Pump B A3 G3 Pump A G4 On-line filter Mixer G2 B3 New capillary of 180 mm ph Flow cell G5 (Bypass) Injection Valve (V1) Column Fraction Collector NC NO NC NO Sample loop A2 A1 B2 B1 W2 W W Outlet Valve (V4) 4 5 G6 (H6) COND UV G8 (H8) New capillary of 130 mm Flow restrictor G9 (H9) ÄKTApurifier F3 um 4
6 P ml/min25MPa UV nmUV-Vis3 10mm 8µl 2mm 2µl ph/c-900 ph 5
7 6 Frac mm mm 4030 mm 3 Frac-950 XY4 A & 30 mm B mm C mm 8 D 4530 mm Frac-900 Frac-950 V1 37 Load 3 Inject Waste PumpWash Column Sample syringe Pump Column Pump Column Pump Waste Load Waste Inject Waste Waste Waste um 6
8 1 V µl 100 µl 1 ml 150 ml 10 ml 50 ml 150 ml 3 FR MPaFR MPa2 FR-902 FR-902 FR µm 2M NaOH
9 1.2 ÄKTApurifier & 500 ml 20 % ÄKTA % Evaluation ÄKTA 9 10 um 8
10 UNICORN ÄKTA 21 1 CU-950 PC4 1. CU ÄKTA ONPC OFF ON 3. ON Login Windows 4. Windows UNICORN 5. Logon User name default Passworddefault 6. ÄKTA UNICORN Manager 9
11 6. ÄKTASystem Control Run data Instruments Ready CU-950 LEDPower PC System 3 um 10
12 2.2 UNICORN UNCORN UNICORN 4UNICORN Manager Method Editor System Control Evaluation 6 UNICORN Manager Copy, Rename, Delete, Move Method Editor System Control Evaluation Instant Run Logon/Logoff UNICORN Logon/Logoff Method Queue Method Queue Editor Display running Method Queue 11
13 Close Manual Manual 5-2 Flowpath Manual Flowpath Pump A Inlet Manual Flowpath... Pump A Inlet 5-4 A2 Manual Flowpath... Pump A Inlet A2 5-5 Execute um 12
14 3 3 System ControlManual 3.1 W1 5 W2 4 W3 1F3 PEEK4 F3 F2 F3 W % A B
15 A1, A2, B1, B A1, B1 2 A ml 5 A 2 4 A1 6B2 7 A2, B2 Manual Flowpath... PumpAInlet A2 Execute A1A2 Manual Flowpath... PumpAInlet B2 Execute B1B A2, B2 1 A1 um 14
16 3.4 3 UV 1 System Control Run Data Curves 2 AA1 Manual Pump... Flow 5 ml/min Execute Pressure 3 BB1 Manual Pump... Gradient Target 100 %B, Length 0 min Execute Pressure 4 BB2 Manual Flowpath... PumpBInlet B2 Execute 5 AA2 Manual Pump... Gradient Target 0 %B Length 0 min Execute Manual Flowpath... PumpAInlet A2 Execute 6 End A1, A2, B1, B20.05MPa 3.5 PumpWash 1 System Control 25 Manual Pump... PumpWash Purifier ON, ON, ON, ON Execute PumpWashContinue Pause Continue PumpWash wastew1 15
17 UV G5 2. Manual Pump... Flow 5ml/min Execute 5UV Cond Pressure 3. END LoadG5 UV ph W3 um 16
18 ÄKTAdesign1/16HiTrap FPLC HR M HiTrap HiTrap1/16 PEEKÄKTApurifier0.5 mm i.d. PEEK0.25 mm i.d. PEEK TricornRESOURCE1/16 M6 1/16 1/16 M6 ÄKTAdesign HiTrapHiLoad FPLC HRM6 Union M6 female / 1/16 male PEEK Union 1/16 female / M6 male PEEK Union 1/16 male / M6 female PEEK Union 1/16 male / Union 1/16 male PEEK Fingertight connector, 1/16 male PEEK Stop plug, 1/16 male PEEK Union, Luer female 1/16 male PEEK SRTC-2, M6 female (1.5 mm i.d.) PEEK Tubing (0.25 mm id, blue) PEEK 2 m Tubing (0.50 mm id, orange) PEEK 2 m Tubing (0.75 mm id, green) PEEK 2 m Tubing (1.0 mm id, beige) PEEK 2 m
19 PV-908 INV HiTrap HiPrep UNICORN Manual Alarms&Mon... Alarm_Pressure High Alarm Execute 1 Manual Pump... Flow 0.5 ml/min Execute 2 UV 3 END % 1 Pressure limit Manual Alarms&Mon... Alarm_Pressure High Alarm Execute 2. 20% Manual Pump... Flow 1/2 Execute 1/4 33UV Cond Pressure END um 18
20 4 HiPrep ÄKTA HiPrep 0.15MPa ÄKTA 0.35 MPaHiPrep0.15 MPa FR MPa HiTrap ÄKTA HiTrap 0.5 MPaHiTrap0.3 MPa FR MPa Manual Pump... Pumpwash Purifier ON, OFF, ON, OFF Execute 1 A1 2 A1/B1 BufferPrep A1 Buffer A2 Acid/Base B1 Water B2 Salt 19
21 20 um mm Sample syringe Waste Column Waste Pump Inject Column Waste Pump Load Column Waste Waste Pump
22 Superloop 150 ml 50 ml 10 ml Superloop 10 ml 50 ml 4 MPa Superloop 150 ml 2 MPa 21
23 6 6 Frac-950 Frac Frac DropSync 2 ml/min Accumulator accumulator 2 ml/min Accumulator Tube DropSync Accumulator OFF System Control System Settings... Specials FracParameters um 22
24 V4Frac ml/min DropSync Tube 1 OutletValve F2 Accumulator A 25 mmc 3 AccumulatorPEEK A 2 ml/min Accumulator 1. OutletValve F2 Accumulator B cm PEEK 5 mmc A B C Keying guide Knurled screw 23
25 mm 18mm 30mm 96 30mm 18mm 30mm 2 ml/mindropsync2 ml/minaccumulator Rack A 18mm 15 ml 1 Rack B 12mm 5 ml Rack C 96 2 Rack D 30mm 50 ml Greiner high Nunc low Greiner PP Masterblack 2ml 96well um 24
26 AccumulatorpH System Control System Settings... Specials FracParameters Accumulator ph Cell ph Cell 0.25 mm 238 µl 0.5 mm 382 µl 527 µl 0.25 mm 88 µl 0.5 mm 232 µl 354 µl PEEK ph Cell PEEK mm ) µl / 10 cm PEEK PEEK ÄKTApurifier 25
27 6 6.2 Frac Pause Continue um 26
28 mm 6 PEEK5 mm 27
29 7PEEK mm mm 12 mm Eppendorf tube holder for Tube Rack mm 1.5 ml 0.5 ml 30 mm um 28
30 ph System Control System Settings... Specials FracParameters_900 ph Cell ph Cell 0.25mm 88 µl 0.5mm 232 µl 354 µl PEEK ph Cell PEEK mm ) µl / 10 cm PEEK PEEK ÄKTApurifier 29
31 7 Wizard 1 Wizard Wizard RESOURCE S 1 ml 4 ml/min 100 µl 100 µl Segmented Gradient Advanced 50 %B 10 CV 280 nm 254 nm Frac-950 Frac Wizard Method Editor File New... New Method Wizard OK um 30
32 7 7.2 Main Selection Affinity Anion Exchange Cation Exchange Chromatofocusing CIP HIC Installation test RPC Size Exclusion Cation Exchange Column RESOURCE S 1 ml Flexible Flow Rates 3 Variables Flow Regulation of the System Pump Flexible Flow Rate BufferPrep phon / OFF Next 31
33 7.3 WaveLength UV1 WaveLength UV2 WaveLength UV3 Pump A Inlet A A1 Pump B Inlet B B1 Next 280 nm 254 nm 215 nm 7.4 Equilibration Start Concentration B B% 0 %B Equilibration Volume Watch Equilibration CV 3 5 CV Conductivity, ph, UV OFF Next um 32
34 7 7.5 Injection Technique Manual System Pump Direct Loading Manual Empty Loop With Next Frac-950 Frac Frac-950 Setting / Flowthrough Fractionation Frac-950 Rack 18 & 30 mm Tube Wash Out unbound Sample [2 CV] 2 CV Variables 7.10 Flowthrough Fractionation Frac-950/OutletValveF3/OFF Frac
35 Fraction Volume Tube Type Start at Next tube First tube 18 mm30 mm 1 Fraction Volume 50 ml Tube Type 30 mm Start at First Tube Next Elution Fractionation Frac-950 OFF OutletValve Frac-950 OutletValve Frac-950 Frac Fixed Volume and Peak Fractionation Fixed Volume Fractionation Peak Fractionation Fixed Volume Fractionation Fraction Volume Tube Type Start at 18 mm30 mm Fraction Volume 1 ml Tube Type 18 mm Start at First Tube Next um 34
![7 7.6.2 Frac-900 Wash Out Unbound Sample Wash Out unbound Sample [2 CV] 2 CV Variables 7.](/docs-images/104/164142099/images/36-1.jpg "10 Flowthrough Fractionation Frac-900/OFF/OutletValveF3 OutletValveF3 Frac-900 Fraction Volume Next Elution Fractionation Frac-900 Frac-900 OFF OutletValve OutletValve Frac-900 Frac-900 3 Fixed")
36 Frac-900 Wash Out Unbound Sample Wash Out unbound Sample [2 CV] 2 CV Variables 7.10 Flowthrough Fractionation Frac-900/OFF/OutletValveF3 OutletValveF3 Frac-900 Fraction Volume Next Elution Fractionation Frac-900 Frac-900 OFF OutletValve OutletValve Frac-900 Frac Fixed Volume and Peak Fractionation Fixed Volume Fractionation Peak Fractionation Fixed Volume Fractionation Fraction Volume 1 ml Next 35
37 7.7 Elution Elution Technique Isocratic Isocratic Isocratic with Delayed Fractionation Linear Gradient 1 Segmented Gradient Advanced 9 Step Gradient StepWize Gradient Segmented Gradient Basic 9 Gradient Segmented Gradient Advanced Gradient Segments 2 Reequilibration after Elution (5CV) 5 CV Next um 36
![7 7.8 Segment 1 12 Segment 1 1 Elution-Segment 1[2] --- Page 1[2] Step / Gradient Gradient Pump Inlet A and B A B A1 B1 Target Concentration B Segment%B100% Length of Gradient Segment CV20CV.](/docs-images/104/164142099/images/38-0.jpg "..................Gradient Pump Inlet A and B..... A1,B1 Buffer Valve A Inlet...... A11 Target Concentration B...50% Length of Gradient.")
38 7 7.8 Segment 1 12 Segment 1 1 Elution-Segment 1[2] --- Page 1[2] Step / Gradient Gradient Pump Inlet A and B A B A1 B1 Target Concentration B Segment%B100% Length of Gradient Segment CV20CV Gradient Pump Inlet A and B..... A1,B1 Buffer Valve A Inlet A11 Target Concentration B...50% Length of Gradient CV Next Segment 1 2 Elution-Segment 1[2] --- Page 2[2] Segment 1Wash Flush Wash Flush [Segment 1] Wash Inlet A APumpWash 1 37
39 Wash Inlet B BPumpWash 1 Flush System with Set Concentration B 5ml A,B 1 ConcB% Fraction Volume Segment Fraction Volume Wash Inlet A Wash Inlet B Flush System with Set Concentration B 5ml... Fraction Volume ml Next um 38
![7.9 Segment 2 7 Segment 2 1 Elution-Segment 2[2] --- Page 1[2]...................Step Pump Inlet A and B...... A1,B1 Target Concentration B...100% Length of Gradient.](/docs-images/104/164142099/images/40-0.jpg "......3CV Next Segment 2 2 Elution-Segment 2[2] --- Page 2[2] Wash Inlet A............................... Wash Inlet B............................... Flush System with Set Concentration B 5ml.")
40 7.9 Segment 2 7 Segment 2 1 Elution-Segment 2[2] --- Page 1[2] Step Pump Inlet A and B A1,B1 Target Concentration B...100% Length of Gradient CV Next Segment 2 2 Elution-Segment 2[2] --- Page 2[2] Wash Inlet A Wash Inlet B Flush System with Set Concentration B 5ml... Fraction Volume ml Finish 39
41 7.10 Variables Wizard VariablesValue Variables Wash Out Unbound Sample Reequilibration Flexible Flow Rates Variables Show details um 40
42 7.11 Run Setup 7 Frac-950 Rack Fraction order Wizard 4 Gradient Xmin / ml / CV 41
43 7.12 File Save As... Method Name Technique OK 001Result 7.13 UNICORN Manager Method um 42
44 8 8 System Control µm ph Load3 3 Column Sample syringe Pump Column Pump Column Pump Waste Load Waste Inject Waste Waste Waste
45 8.3 Frac Frac-900 Outletvalve F3F System Control File Run OK 3. Next 4. Frac-950Rack Fraction order 5. Start Next Start um 44
46 ENDEnd Method 2. save partial resultok View Panes Rundata Curves OK 3. 45
47 Run Data 1. Run Data 2. Properties Run Data Groups New Group 5. OK um 46
48 8.7.2 Curves 8 1. Curves 2. Properties Curves 3. Select All 4. OK 1. Curves Marker XY 4. XY 5. Marker 47
49 1. Curves Properties 2. Curve Style and Color OK Y Y 1. Curves Properties 2. Y-Axis 3. Fixed OK um 48
50 X 8 XTime minvolume ml 1. Curves Properties 2. X-Axis 3. Time minvolume ml 4. Total WindowTotal Window 5. OK Undo Zoom 5. Reset Zoom 49
51 View Panes Flow Scheme Logbook 1. View Panes Logbook Result um 50
52 8.8 8 System Control Run Hold Pause Continue End Continue Continue Pause Hold Pause 1. Manual Execute 51
53 Evaluation 2. File Open Result Open Result OK um 52
54 Properties 3. Chromatogram Layout Chromatogram Layout Curves OK 53
55 9.2.2 Y 1. Chromatogram Layout Y-Axis AutoFixed 4. 3UVAll with this unit 5. OK X 1. Chromatogram Layout X-Axis 2. Auto Fixed 3. Adjust retention zero to injection number 0 min ml 4. OK um 54
56 Page Down Page Up Reset zoom 55
57 Add Text abc 3. abc 4. texttext Edit Text Mode File Print... Print Chromatograms 2. Print Format 3. Landscape 4. Preview...Customise Report Exit 5. OK um 56
58 % 10.1 System Control 1. Manual Pump... PumpWash Purifier ON, OFF, ON, OFF Execute Pressure limit Manual Alarms&Mon Alarm_Pressure High Alarm Execute 2. Manual Pump Flow Execute 3. 3UV, Cond, Pressure END 4. UVG5 5. Manual Flowpath Injection Valve Inject Execute Manual Pump Flow 5ml/min Execute 6. 5END 10.3 Frac Manual Pump Flow 2ml/min Execute 2. Manual Flowpath OutletValve F2 Execute 3. Accumulator Accumulator Manual Frac AccumulatorWash Strokes 5 Execute 4. Accumulator Manual Flowpath OutletValve F3 Execute 5. 3END 57
59 Frac Manual Pump Flow 2ml/min Execute Manual Frac Fractionation_900 FracSize 2ml Execute 4. Manual Frac Fractionation_Stop_900 Execute 5. Manual Flowpath OutletValve F3 Execute 6. 3END um 58
60 UNICORN Manager 2. File Quit program 3. UNICORN Manager YES 4. System ControlLeave Control of System1OK 5. Windows Start Turn off Computer Turn off ÄKTAexplorer 6. OFF OFF 59
61 %20% 20% ph ph 1. 20% 2. System Control Manual Pump... PumpWash Purifier ON, OFF, ON, OFF Execute 3. Pump Wash Manual Pump... Flow 5ml/min Execute 4. 5END % um 60
62 µm 1 ml / min 0.35 MPa2M NaOH Frac
63 HDDOS-V 1.44MB 1. A 2. WindowsMy Computer /2 Floppy (A:) 4. Format Start 6. OK 7. Format completeok 8. Close 9. My Computer um 62
64 12.2 Copy to External UNICORN Manager Methods Results 2. Copy to External 3. Copy 3 1/2 Floppy (A:) Save Copy to External Result zipzip Copy from External 12.3 UNICORN CD 1. CD CD 2. OK 3. CD Delete temporary files 63
65 4. My Computer CDUNICORN Result C:/UNICORN/Local/Fil CD C:/UNICORN/Local/Fil/Defaut /Result Manual Runs 5. CD Write these files to CD 6. CD Writing Wizard CD namenext 7. Finish um 64
66 12.4 Copy from External UNICORN Manager Methods Results Copy from External Copy: 3 1/2 Floppy (A:) 5. OK 6. ResultResults 7. MethodMethod-System Connection 8. Method filesystems system 1 9. OK Copy from ExternalzipCopyzip 65
67 UNICORN Manager Methods Results 2. Ctrl 3. Delete 4. OK 12.6 Rename 1. UNICORN Manager Methods Results 2. Rename 3. OK um 66
68 ÄKTApurifier Pause Continue No Yes Check 2 END 67
69 ÄKTApurifier A2 0.00MPa 0.05MPa No Yes um 68
70 ÄKTApurifier Gradient ; ConcB% 0% Flow ml/min Execute MPa Yes No 0.15MPa Check MPa Gradient; Conc%B 0% InjectionValve Waste Execute 0.10MPa No Yes Check 3 Act 1 69
71 CU-950 CU-950 CU-950 ÄKTA 1 ÄKTA 1. Uninet ÄKTA CU-950 Uninet 2 ÄKTA CU USB CU-950 USB USB USB 3. CU-950 CU CU-950 Power PC System ÄKTA 4. ÄKTA ÄKTA Windows CU-950 Power PC System PC 2 5. UNICORN ÄKTA UNICORN ÄKTA CU-950 Power PC System PC CU-950 USB CU-950 System Uninet CU-950 'Test' 'Network' 'UniNet-1' Input 24-32V 1A USB UniNet-1 Test 3 Fraction Collector Frac-920 Frac-920 Frac Frac Frac-920 ÄKTA Setup and Check ÄKTA UNICORN Never 'Properties' Display Properties Screen Saver 'Power...' Power Options Properties Plugged in System standby 'Never'
72 UPC-900 ÄKTApurifier UPC 10/ nm 254 nm 2. Hg 280 nm 254 nm UV OFF 1. UPC Lamp ON/OFF OK OFF OK Lamp (on) Lamp (on) on off UPC-900 System Control System Settings Specials Keyboard Mode Open OK memo
73 Home Page (CGP)
74 60
75 OFF TEL : : : 30
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