248 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /-, No./,,.2,/. (,**0) 12 * * * Microencapsulation of Glutamine with Zein by a Solvent Evaporation Metho

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1 248 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /-, No./,,.2,/. (,**0) 12 * * * Microencapsulation of Glutamine with Zein by a Solvent Evaporation Method in Reverse Multiple Emulsion Masato Tanaka*, Yoshinari Taguchi* and Chieko Shima Department of Science and Technology, Graduate School, Niigata University,,2*/* Ikarashi, Niigata-shi, Niigata 3/*,+2+ * Department of Chemical Engineering, Niigata University,,2*/* Ikarashi, Niigata-shi, Niigata 3/*,+2+ We prepared zein microcapsules containing glutamine using a solvent evaporation method in reverse multiple emulsion. In the experiment, fatty acids were added to the shell material to give the shell hydrophobicity ; as a result, zein microcapsules containing glutamine were successfully prepared by the microencapsulation method adopted here. It was found possible to change the release rate of the core material by addition of fatty acids. Moreover, it was found that the addition of oleic acid resulted in the strongest hydrophobicity and that the use of palmitic acid had the greatest e#ect on the content of the core material. (Received Sep.,2,,**/ ; Accepted Feb. +-,,**0) +, -/ 3/*,+2+, 2*/* * 3/*,+2+, 2*/* Corresponding author tanaka@eng.niigata-u.ac.jp PH Zein +

2 13 : 249 L Gln OA SA + LA PA + DBS +, Fig. + 2./ cm, /* cm - /.* cm cm, +.* cm. Fig., Gln SO ; solid-in-oil. g 2 g, s + 3* vol 0 SO -.- s + SOW SOW -* /.* s + SOWO, DBS +* Table + Fig. - SO Fig., Flowchart of microcapsule preparation Table + Experimental conditions Experimental conditions Fig. + Schematic diagram of experimental apparatus Volume of continuous phase Amount of Gln Amount of Zein Volume of solvent Lecithin Impeller speed Amount of Additives : +2 cm - :,4/ g :.4* g : +0** cm - : / g : -4- s + : *+4, g

3 250 /- /,**0 / 14 Fig. - Mechanism of microencapsulation - Fig...*,/ + mm +** cm - H L q / Fig.. qdegree,tan +,HL W A W AW nw OW O W O W n -** Dp Sautar Dpm ndp- ndp, Fig.. Schematic diagram of preparation and estimation of Zein film VM-+A, CBVP-A- SEM, JSM-/2** SEM /* mg +* cm -,* HPLC GL Inertsil ODS--..0 f+/* mm.* UV,+* nm,,* mm,.. +* - kg ++* - m - ph,./ *.1 mlmin F F Gln Gln +**

4 ῌ : (a) Oleic acid (b) Stearic acid (d) Palmitic acid (c) Lauric acid Fig. / E#ect of additives on contact angle of water droplet with Zein film

5 252 /- /,**0 / 16,/* mg /* cm - Gln HPLC R R Gln +** + Fig. / OA q/2./ 0,., +.0 g SA, LA, PA *..+ SA PA OA 3 LA +, PA +0 SA +2, Fig. 0 W A *.2 g OA OA LA PA SA - Fig. 1 *.. g Dp Dp +0* m SA, LA, OA, PA +3* m, -/* m, -2* m, /** m g Table,. Fig * PA / Fig. 3 *.. g Fig. 0 E#ect of additives on water absorption of Zein film Fig. 1 E#ect of additives on mean diameter of microcapsules

6 17 : 253 Table, Interfacial tension and viscosity at the addition of *4. g Non SA LA OA PA g N/m Pa s.4*+* o. *4,- -4,+* o. *4,0-4/+* o. *4-* -41+* o. *4-/ -40+* o. *4.+ Fig. 2 E#ect of additives on content of core material Fig. +* SEM photographs of microcapsules Fig. 3 E#ect of additives on release of core material PA PA 0 Fig. +* SEM OA SEM Fig. ++ OA Fig. ++ SEM photographs of surface of microcapsule

7 254 /- /,**0 / ,**-,, +33* ,* ,/* +332 / /, 2*21,**/ *0-+, * +,./ +33/ +1 3,2 +2, +-

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