Vol.42 No.1/◇0 目次[三]3.2
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3 Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1 1 1, Sakuragaoka, Setagaya-ku, Tokyo Hitachi Health Management Center, , Osecho, Hitachi-shi, Ibaraki Alpha Food Co., Ltd., 645, Kitaaraki, Taisya-cho, Izumo, Shimane Key wordspregelatinized brown rice, cholesterol, CYP7A1, obesity, Zucker fatty rat αα
4 γ γ α α α α α α α αα αα α αα α tert
5 α et al µ µ α β p p αα p
6 α p p p p α p p α β pp p p p p p
7 α β p p αα α α α α α α α α αα Lancet
8 Am J Cardiol Lancet Am J Clin Nutr Nutr Res. Phytother Res γ Diabetes JNutri α α J. Biol. Chem Diabetes Metab Res Rev Eur J Nutr Biosci Biotechnol Biochem Prev Nutr Food Sci
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13 Food Processing Center, Faculty of Applied Bioscience, Tokyo University of Agriculture, Sakuragaoka, Setagaya-ku, Tokyo Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agriculture, Sakuragaoka, Setagaya-ku, Tokyo Key wordsdough, bread, gliadin, protein disulfide isomerase, disulfide bond E. coli E. coli -
14 µ µ Ex Em
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16 µ ω µ µ µ
17 Bulletin of Tokyo Food Tech. Res Food Preser.Sci Food Preser.Sci Escherichia coli, Food Preser.Sci Food Preser.Sci J. Bio. Chemi J. Bio. Chemi Proc. Natl. Acad. Sci. USA Cereal Chem., J Food Sci, Anal Biochem β Food Preser. Sci
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19 The Yamanashi Prefectural Industrial Technology Center, 2094 Otsumachi, Kofu-shi, Yamanashi Food and Nutrition, Yamanashi Gakuin junior college, Sakaori,Yamanashi Health and Nutrition, Yamanashi Gakuin University, Sakaori, Yamanashi Graduate school, Course of Functional Studies of Basic Necessities for Living, Showa Women s University, Taishido, Setagaya-ku, Tokyo Momo Hanamomo Hanamomo Momo Hanamomo Momo Momo Hanamomo Momo Hanamomo Hanamomo Hanamomo Hanamomo Key wordspetals of edible peaches Momo, petals of ornamental peaches Hanamomo, anthocyanin, cyanogenic compound, prunasin
20 Momo Hanamomo Momo Hanamomo Momo Hanamomo Momo Hanamomo
21 µ β µ µµ µµ µµ
22 Momo Hanamomo Momo Hanamomo Momo Hanamomo Momo Hanamomo Momo Hanamomo Momo Hanamomo Hanamomo
23 f f Momo Hanamomo Momo Hanamomo Momo Hanamomo µ µ Momo Hanamomo Momo Hanamomo
24 J. Exp. Bot
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26 Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1 1 1, Sakuragaoka, Setagaya-ku, Tokyo Key wordspolyphenol, hydroxytyrosol, olive, intracellular kinetic analysis
27 Olea europea N N µ µ HO O OH HO O OH HO HO 01 OH + SCN 02 O O DMF 120 C HO HO O S N H O O 03
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29 26 日本食品保蔵科学会誌 VOL 4 2 NO 1 2 0 1 6 26 a b c d e f g h i j k l Fig 3 FITC-HTのCaco-2細胞内におけるミトコンドリアへの局在性の検討と動的イメージング Caco-2細胞をFITC-HTで処理した後に ミトコンドリアマーカー MitoTracker および核マーカー Hoechst3 3 34 2 で30 分間反応後 生細胞イメージング装置 KEYENCE BZ-X7 0 0 を用いて 6時間イメージングムービーを取得した 位相差画像 b FITC-HT 緑 画像 c MitoTracker 赤 画像 d Hoechst33 3 42 青 画像 e l 重ね合わせ画像のタイムラプス画像 スケールバー 5 0! a b c d e f g h i j Fig 4 FITC-HTおよびフルオロセインのCaco-2細胞への取り込み Caco-2細胞をFITC-HTで処理した後に リソソームマーカー LysoTracker で反応させ 固定した その後に 核マー カー Hoechst3 3 3 4 2 で反応後 共焦点レーザー顕微鏡 OLYMPUS FV12 0 0 を用いて観察した FITCの構造 b FITC-HT 緑 画像 c LysoTracker 赤 画像 d Hoechst3 33 4 2 青 画像 e b e の重ね合わせ画像 f フルオロセインの構造 g FITC-HT 緑 画像 h LysoTracker 赤 画像 i Hoechst3 3 3 4 2 青 画像 HTが最適であると考えた そこで HTをFITCで標識 でドット状に散在していること その小胞状部分は 一 することにした その結果 高純度の標識化合物を得る 部リソソームと共局在していた Tokyo Greenという ことに成功した 次に この化合物が細胞内に取り込ま 蛍光発色団で標識したエピガロカテキンガレート れるかどうかを確認した その結果 FITC-HTがCaco- EGCG を用いて ヒト臍帯静脈内皮細胞 HUVEC 2細胞内に取り込まれるものの 核への局在は観察され における細胞内動態を検討したところ 細胞内にドット なかった HTが核に局在するという報告はないことか 上に取り込まれるという報告があり13 14 本結果と類似 ら 本結果とHTの挙動は類似しているものと示唆され したものであった したがって HTとEGCGは 細胞 た また 細胞内へ取り込まれたFITC-HTは 細胞内 内で同様の動態を示す可能性があると考察した 一方
30 in vivo Micron Methods Cell Biol Life Sci Planta Med J Ethnopharmacol J Nutr Sci Vitaminol Tokyo J Nutr Á J Nutr Biochem Food. Chem. Toxicol
31 α Nutr Cancer Bioorg Med Chem Lett Biol Pharm Bull Bioorg Med Chem Chem CommunCamb
32 National Food Research Institute, NARO, Kannondai, Tsukuba-shi, Ibaraki Key words fresh produce, modified atmosphere packaging, cushioning packaging, quality MA
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35 Food Preservation Science Transactions of the ASAE Society of Agricultural Structures, Japan Journal of Food Research Postharvest Biology and Technology Packaging Technology and Science Japan Agricultural Research Quarterly Packaging Technology and Science Japan (SASJ) Journal of Food Engineering
36 Agriculture & Environment Agriculture & Environment Food Preservation Science Journal of Packaging Science & Technology, Japan
Fragaria x ananassa Bioengineering College of Jimei University, Jimei, Xiamen 361021, Fujian Province, China Department of Food Science and Technology, Tokyo University of Marine Science and Technology,
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