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1 Weigert's Iron Hematoxylin Staining Set 1set 12, % 96% 1 2% 0.25%, 1: Weigert's Resorcin Fuchsin Solution 3,600 1g 2% 100ml 70% 400ml 2% 70% Van Gieson Solution F 1% Fuchsin Acid Solution 3,500 5g Van Gieson Solution P Picric Acid Solution, Saturated 2,600 P F 100ml15ml WAKO BIO WINDOW. 1999

2 SX % G G 5 61% p.27 No Aniline Blue Solution 1,800 No Immunopen WAKO BIO WINDOW ,200 4,000

3 , G 0.75% G 50ml Aniline Blue Orange G Solution 0.5g G 2g Azocarmine G Solution G 0.1g 100ml 1 10 G G G 7 4, ml 8ml 3,500 1ml WAKO BIO WINDOW. 1999

4 4. PTAH Phosphotungstic Acid Hematoxylin Solution PTAH Solution 100ml 2,500 5,500 1g 20g 1,000ml 5. Berlin blue stain, Prussian blue stain Berlin Blue Staining Set 1set 3,800 2% 1 2% 1 WAKO BIO WINDOW. 1999

5 6. OG-6 GEA-50 Y SF G Y SF 5g 44g 730ml 0.52g orange G 5g 95% 1,000ml 0.15g 0.1% 10% lightgreen SF yellowish 2ml 95% 198ml 0.5% 10% eosin Y 10ml 95% 190ml 0.5% 10% Bismarck brown 2.5ml 95% 47.5ml 45ml 45ml 10ml 0.2g 1 WAKO BIO WINDOW. 1999

6 No PapanicolaouGill' s Hematoxylin Gill-5Stain Solution Papanicolaou OG-6 Stain Solution Papanicolaou EA-50 Stain Solution 3,200 3,200 3,200 EA100, OG100, No PapanicolaouHematoxylin Stain Solution Papanicolaou EA-100 Stain Solution Papanicolaou OG-100 Stain Solution 2,300 2,800 2, ml ml B Y WAKO BIO WINDOW. 1999

7 No Wright Stain Solution May-Gr unwald Stain Solution Giemsa' s Stain Solution 1,400 1,250 2,300 MGE, ml 3 No May-Gr u nwald' s Eosin Methylene Blue Solution 2,600,, p WAKO BIO WINDOW. 1999

8 8. 500mg 70% 100ml 70% m No Villanueva Bone Stain Reagent Villanueva Bone Stain Solution 500mg 10 15,000 9,500 1Villanueva, A. R. et al. : Stain Technology, 49, Villanueva, A. R. et al. : Am. J. Med. Technol., 43, WAKO BIO WINDOW. 1999

9 . 750cps 550cps 2 550cps Ap A No Protein Digestion Enzyme TdT Softmount Softmount 550 TdT Substrate Solution 100 POD-Conjugated Antibody cps 550cps 1. in situ 1ml 40 l 4.4ml 44 l DAB Solution DAB Enhancer DNase 10 DNase Reaction Buffer HE HE ,000 7,000 TUNEL TdT-mediated dutp nick end labeling 4.4ml 200 l 4 l 40 l 2 T cell HE DAB No Apoptosis in situ Detection Kit wako 40 60,000 WAKO BIO WINDOW. 1999

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