遺伝子組換え技術を用いたユニフォームポリマーの合成
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1 1 Synthesis of Uniform Polymers with Recombinant DNA Technique Kazuhisa Yano p / = 1 + p 2 M w ( / = 1 ) 1956 Szwarc / = 1.03 M w M w M w M n M n M n M n M w M n DNA
2 Natta AlEt 3 -TiCl 4 Ziegler TiCl 4 TiCl 3 Ziegler- NattaZiegler-Natta Natta TiCl 3 AlEt 2 Cl (PP) Montedison MgCl 2 Ti Classification of polymerization. Difference of molecular weight distribution between a conventional polymer and a living polymer.
3 3 Kaminsky AlMe 3 (Al(Me)O) TiCl 4 Ti(OEt) 4 CpTiCl 3 Ti 100 ( 267 ) ( 225 ) β Stereoregularity of polymer F. Miescher ( (G) (A) (C) (T) ) 1929 DNA RNA 1928 F. Griffith S S R S O. T. Avery DNA DNA 1953 J. D. Watson F. H. C. Crick DNA DNA 1960 DNA DNA DNA restriction endonuclease 1970 Smith Haemophilus influenzae d DNA
4 4 Structure of DNA. Watson-Crick model of DNA. Boyer EcoR I Roberts DNA 1970 Mandel Higa DNA λ 2Cohen R6-5 DNA DNA 1972 ClewellE1 ColE
5 5 DNA ColE1 Cohen psc101 DNA RSF2124 ColE1 ( 7.8MDal ) 1977 Bolivar R7268 Cleavage with restriction endonucleases. RSF2124 psc101 ColE1 pmb1 pbr Cohen Boyer Boyer EcoR I R6-5 Cohen psc101 2 DNA 1 DNA 2 DNA DNA C600 psc101 R6-5 Staphylococcus aureus p1258 Structure of pbr322. psc101 1 Xenopus laevis RNA psc101 DNA 1973
6 6 mrna DNA α β Cloning of tetracycline resistent plasmid.
7 7 α β 30 3 (GlyProHyp) Gly ProHyp 20 (GlyProPro) Salerno GGT CCG 2 ( 18 ) DNA 32 DNA Principle of the synthesis of uniform polymers. Potential problems in biosynthesis.
8 8 9 β Bombyx mori ( GlyAlaGlyAlaGlySer ) Ferrari 6 DNA 160 DNA DNA Cappello ( GlyAlaAlaValThrGlyArgGlyAspSerProAla- SerAlaAlaGlyTyr ) 6 1 ( ValProGlyValGly ) Waite Structure of protein.
9 9 Salerno Mytilus edulis 10 AlaLysProSerTyrHypHypThrDopaLys Dopa = 3,4-dihydroxy phenylalanine; Hyp = Hydroxy proline Dopa Hyp Phe Pro λpl E. coli K12 IG110 T7 E. coli BL DNA α β DeGrado C 4 α ( GlyGluLeuGluGluLeuLeuLysLysLeuLysGluLeu- LeuLysGly ) E. coli Lawton ( AsnLeuAlaPheValAlaGlnTrp ) α 36bp 576bp DNA A Tirrell GlyAla β β β (AlaGly) 3 ProGluGly E. coli 100mg / 10L (AlaGly) β ProGluGly β (AlaGly) 4 - ProGluGly X β 30A Structure of four helix bundle. Monomer DNA sequence of [ (AlaGly) 3 ProGluGly ] n.
10 10 β β (AlaGly) 3 GluGly X β ProGluGly GluGly (AlaGly) Strategy for cloning and expression of [ (AlaGly) 3 ProGluGly ] n.
11 11 Expected structure of [ (AlaGly) 3 ProGluGly ] n γ- α L- PBLG α γ- α L- HGluAsp(Glu 17 Asp) 4 GluGluOH E. coli / α L- M w M n PLGA) 20 (SeMet) (GlyAla) 3 GlySeMet SeMet SeMet 100 Comparison of molecular weight distribution of HGluAsp(Glu 17 Asp) 4 - GluGluOH with two commercial PLGAs on a 12% polyacrylamide gel. Lane 1, PLGA of molecular weight 9050 and polydispersity Index 1.20; Lane 2, HGluAsp(Glu 17 Asp) 4 GluGluOH; Lane 3, PLGA of molecular weight 21,000 and polydispersity Index ) P. J. Flory (1980) 304 2) Szwarc, M., et al. : J. Am. Chem. Soc., (1956), ) Miyamoto, M., et al. : Macromolecules, (1984), 265 4) Faust, R. and Kennedy, J. P. : Polym. Bull., (1986), 317
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Database Center for Life Science Online Service Vol.48 No.16 (2003)
Database Center for Life Science Online Service Key words Kikuya Kato, E-mail : kkato@bs.aist-nara. ac.jp http://love2.aist-nara.ac.jp Shin Ishii, E-mail : ishii@is.aist-nara.ac.jp http://hawaii.aist-nara.
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