PAXgene Tissue DNA Kitプロトコールとトラブルシューティング( /2009)

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1 January 2009 PAXgene Tissue DNA Kit PAXgene Tissue Containers DNA PAXgene Tissue Container

2 PAXgene DNA 3 PAXgene DNA 6 PAXgene DNA PAXgene Tissue DNA Kit 01/2009

3 PAXgene DNA DNA PAXgene Tissue Containers 2 5 PAXgene Tissue Container Product Circular 5 10 µm 100 mm 2 DNA DNAPAXgene DNA Spin Column DNA PAXgene Tissue Container Product Circular Buffer TD1 Buffer TD2 56 Buffer TD3 Buffer TD purity grade p.a PAXgene Tissue DNA Kit 01/2009 3

4 µm ml µl µl purity grade p.a ,000 x g 20,000 x g 6. Proteinase KPAXgene Tissue DNA µl Buffer TD1 20 µl Proteinase K ,400 rpm RNA DNA4 µl RNase A 100 mg/ml ,400 rpm ml 4 PAXgene Tissue DNA Kit 01/2009

5 µl Buffer TD2 15 Buffer TD2 RNA Handbook 13 Carrier RNA 1 µg RNA 200 µl Buffer TD2 RNA Buffer TD2 Buffer TD5 Buffer TD µl PAXgene Tissue DNA ml mlpaxgene DNA Spin Column 6,000 x g 1 2 ml * 17. PAXgene DNA Spin Column 500 µl Buffer TD3 6,000 x g 12 ml * 18. PAXgene DNA Spin Column 500 µl Buffer TD4 6,000 x g 1 PAXgene 2 ml ,000 x g 20. PAXgene DNA Spin Column 1.5 ml µl Buffer TD5 PAXgene DNA Spin Column 1 20,000 x g DNA Buffer TD5 PAXgene DNA Spin Column DNA * Buffer TD2 Buffer TD3 Safety information Handbook 6 PAXgene Tissue DNA Kit 01/2009 5

6 PAXgene DNA DNA PAXgene Tissue Containers 20 mg DNA 10 mg DNA PAXgene Tissue Container 20 mg PAXgene DNA Spin Column DNA 2 mm 8 mm mg DNAPAXgene DNA Spin Column DNA PAXgene Tissue Container Product Circular Buffer TD1 Buffer TD PAXgene Tissue DNA Kit 01/2009

7 Buffer TD3 Buffer TD purity grade p.a PAXgene Tissue Container 20 mg10 mg 2 ml 180 µl Buffer TD ml 5 mm 1 TissueLyser Adapter Set 2 x 24 TissueRuptor DNA 3. TissueLyser 15 Hz 20 DNA ml µl Proteome K 1,400 rpm TissueLyser 3 6. RNA DNA4 µl RNase A 100 mg/ml µl Buffer TD2 15 Buffer TD PAXgene Tissue DNA Kit 01/2009 7

8 µl PAXgene Tissue DNA mlpaxgene DNA Spin Column 6,000 x g 1 2 ml * 12. PAXgene DNA Spin Column 500 µl Buffer TD3 6,000 x g 12 ml * 13. PAXgene DNA Spin Column 500 µl Buffer TD4 6,000 x g 12 ml ,000 x g 15. PAXgene DNA Spin Column 1.5 ml µl Buffer TD5 PAXgene DNA Spin Column 1 20,000 x g DNA Buffer TD5 PAXgene DNA Spin Column DNA * Buffer TD2 Buffer TD3 Safety information Handbook 6 8 PAXgene Tissue DNA Kit 01/2009

9 PAXgene DNA DNA PAXgene Tissue Containers 20 mg PAXgene Tissue Container Product CircularDNA 10 mg DNA 20 mg PAXgene DNA Spin Column DNA 2 mm 8 mm mg DNAPAXgene DNA Spin Column DNA PAXgene Tissue Container Product Circular Buffer TD1 Buffer TD2 56 PAXgene Tissue DNA Kit 01/2009 9

10 Buffer TD3 Buffer TD purity grade p.a mg10 mg 2. 2 ml 3. 1 ml ,000 x g 20,000 x g ml purity grade p.a ,000 x g µl Buffer TD ml 5 mm 1 TissueLyser Adapter Set 2 x 24 TissueRuptor DNA 12. TissueLyser 15 Hz 20 DNA ml 10 PAXgene Tissue DNA Kit 01/2009

11 µl Proteinase K ,400 rpm RNA DNA4 µl RNase A 100 mg/ml ,400 rpm ml µl Buffer TD2 15 Buffer TD µl PAXgene Tissue DNA ml mlpaxgene DNA Spin Column 6,000 x g 1 2 ml * 23. PAXgene DNA Spin Column 500 µl Buffer TD3 6,000 x g 12 ml * 24. PAXgene DNA Spin Column 500 µl Buffer TD4 6,000 x g 12 ml * Buffer TD2 Buffer TD3 Safety information Handbook 6 PAXgene Tissue DNA Kit 01/

12 ,000 x g 26. PAXgene DNA Spin Column 1.5 ml µl Buffer TD5 PAXgene DNA Spin Column 1 20,000 x g DNA Buffer TD5 PAXgene DNA Spin Column DNA 12 PAXgene Tissue DNA Kit 01/2009

13 a b Buffer TD2 c Buffer TD3 Buffer TD4 d DNA PAXgene DNA Spin Column A 260 /A 280 Buffer TD2 15 PAXgene DNA Spin Column Buffer TD3 Buffer TD DNA 56 Proteinase K g DNA A 260 /A mm Tris Cl ph 7.5* Handbook 28 Appendix: Determination of Yield, Purity, and Length of DNA DNA A 260 /A 280 RNA RNase * MSDS material safety data sheet PAXgene Tissue DNA Kit 01/

14 DNA a b c DNA DNA a DNA b DNA TissueLyser Buffer TD Buffer TD3 Buffer TD4 Buffer TD4 PAXgene DNA Spin Column 3 PAXgene DNA Spin Column PAXgene DNA Spin Column 1 PCR 100 ng DNA 35 PCR PAXgene TissueLyser 15 Hz PAXgene Tissue DNA Kit 01/2009

15 Trademarks: PAXgene, PreAnalytiX (PreAnalytiX GmbH.); QIAGEN, TissueRuptor (QIAGEN Group). For updated license terms, see PreAnalytiX GmbH, all rights reserved Forefront Tower II Tel: Fax: PreAnalytiX Distributors PreAnalytiX products are manufactured for PreAnalytiX by QIAGEN or BD and are distributed for PreAnalytiX by QIAGEN or BD. Products cannot be ordered at PreAnalytiX GmbH.

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