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1 Vol. 1 (2005) pp * ( ) Approach from Bioelectronics to Bio-Interface Masashi YAOITA * Our studies for approach to bio-interface were fabrication of a needle type glucose sensor for continuous in vivo measurement and investigation of electrochemical cell communication. A micro glucose sensor system of needle type was fabricated. A response current of the sensor was measured in tissue of a goldfish with a needle type glucose sensor system. In this study, we concluded that the needle type glucose sensor system was able to measure about 25 hours in the goldfish tissue, continuously. On the other hand, electrochemical effects of living cells were studied on an electrode. On a potential controlled electrode, we observed burst of cells. From our investigations, cell burst results from inflow of K + and Na + (and H2O) in disorder of K + and Na + channels. These studies suggested that it is possible to use electrochemical devices as cell communication tool. It means that bioelectronics devices could be applied to bio-interface. Key words : Bioelectronics, Biosensor, Cell Communication, Electrochemistry, Bio-Interface 1. * 91
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12 - 10 Y. Ikariyama, S. Yamauchi, T. Yukiashi, and H. Ushioda: Micro-Enzyme Electrode Prepared on Platinized Platinum. Anal. Lett., 20(9) : , Y. Ikariyama, S. Yamauchi, M. Aizawa, T. Yukiashi, and H. Ushioda: High Performance Micro-Enzyme Sensor Using Platinized Microelectrode. Bull. Chem. Soc. Jpn., 61 : , S. Yamauchi, A. Katayama, M. Yaoita, and Y. Ikariyama: Micro Enzyme Electrode with Gold-Black. Technical Digest of the 8th Sensor Symposium : , S. Yamauchi, M. Yaoita, R. Nagai, Y. Yoshida and Y. Ikariyama: Enzyme Embodied Electrode for Cholesterol. Technical Digest of the 9th Sensor Symposium : , S. Yamauchi, M. Yaoita, R. Nagai, Y. Yoshida and Y. Ikariyama: Screening of a Stable Cholesterol Oxidase for the Fabrication of Microbiosensor of Cholesterol by the Embodiment of Cholesterol Oxidase and a Platinized Platinum Electrode. DENKI KAGAKU, 58(12) : , S. Yamauchi, M. Yaoita and Y. Ikariyama: Development of a Pulse Voltammetric Biosensing Device Using an Enzyme Embodied Electrode. Analytical Science, 7 : , M. Yaoita, S. Yamauchi, S. Usami, S. Uchiyama and Y. Ikariyama: Enzyme Embodied Electrode-Based Planer Microbiosensing Device for the Determination of Glucose in Whole Blood. The 7th International Conference on Solid-State Sensors and Actuators, Turans- ducers 93 : , (2)27-32, (1) (6)
13 42(10) M. Yaoita, H. Shinohara, Y. Hayakawa, T. Yamashita, Y. Ikariyama and M. Aizawa : Potential- Controlled Morphological Change and Lysis of HeLa Cells Cultured on Electrode Surface. Bioelectrochemistry and Bioenergetics, 20 : , :. 56: M. Yaoita, Y. Ikariyama and M. Aizawa : Electrically Regulated Cellular Morphological and Cytoskeletal Changes on an Optically Transparent Electrode. Expl. Cell Biol., 57 : 43-51, M. Yaoita, Y. Ikariyama and M. Aizawa : Electrical Effect on the Proliferation of Living HeLa Cells Cultured on Optically Transparent Electrode Surface. J. Biotechnology, 14 : , (8): , : (1996). M. Iwasaka, M. Yaoita, T. Kuno, and S. Ueno : Magnetically Disturbed Activity of Immobilized Catalase on an Electrode in Hydrogen-Peroxide Buffer. IEEE Trans. Magn., 37(4) : (2001).. A121-A(12) : (2001). M. Yaoita, M. Iwasaka, and S. Ueno : Magnetosensitive Enzyme Electrode for Hydrogen Peroxide Sensing. J. Apply. Phys., 93(10) : (2003).. 103(564) : (2004). 103
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