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7 Protophormia terraenovae

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25 Gryllus bimaculatus

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30 Neoditrema ransonnetii

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32 Per

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45 Neoditrema ransonnetii

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48 Per 2AH2AH 2AH 2AH

49 in situ

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54 in vivo dilp6 dilp6

55 Marsupenaeus japonicus Oryzias latipes

56 Per PeriodPer Per Per1b Per3

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58 cyp19a2 cyp19a2 cyp19a2

59 O. latipes éô

60 C. elegans daf-2 daf-2 Pichia pastoris in vitro

61 éô

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63 Hasarius adansoni

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65 Cry3 Cry Cry Cry3Cry3 SgCry3 SgCry3Cry3Cry2 Cry2 in situ

66 Cry3 Cry Cry Cry3Cry3 SgCry3 SgCry3Cry3Cry2 Cry2 in situ

67 periodtimeless Clock ClockClk ClkClk mrna Clk Clk Rhodnius prolixus

68 Gryllus bimaculatus Ehab Hassaneen, Yoshiyuki Moriyama, Kenji Tomioka (Okayama Univ., Grad. Nat. Sci. Tech.) Pigment Dispersing Factor (PDF) is a neuromodulator working in various insect circadian systems. In the cricket, G. bimaculatus, injections of pdf dsrna effectively knocked down the pdf mrna levels. The treated crickets maintained the rhythm both under light-dark cycles and constant darkness. However, they showed rhythms with reduced nocturnal activity with prominent peaks at lights-on and -off. The results suggest that PDF is not essential for the rhythm generation but mediates the nocturnality at the downstream of the circadian clock.

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70 Aplysia kurodai in situ

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72 E Nc E Nc E Nc E Nc

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