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- ともなり とりこし
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1
2 A) " " B a. b. c. i. ii. iii. Bartholomew & Mittwer a. b. a. (Ziehl-Neelsen) b. Truan't Auramine-Rhodamine stain c. "Tbcolor Merck"
3 2. A) 1) 2) (v/v) 3g 30ml 60ml (2% 5ml, 5 ) 3) " "
4 AFB AFB 20ml 20ml 10 AFB ( ) Thimerosol 0.02% 0.50ml 80ml 20ml ( ) B) a Hans Christian Gram Hucker
5 b Davis Davis, J. A.,et al. J.Bacteriol.,156, ,1983. c. i. () ml 1% 1g 2g
6 300ml v/v ml g 100ml 10ml 5 5ml ~20
7 , ii. Hucker 1020% 0.2g 20ml 0.8g 80ml 2g 100ml 20mg 100
8 iii. Bartholomew & Mittwer Bartholomew & Mittwer (B&M)Hucker 5 2 (Hucker ) Hiss halo
9 a % Indian ink( 1%) b. 1 20% % OLT
10 Lembach-Sous
11 a.(ziehl-neelsen)
12 b. Truan't Auramine-Rhodamine stain 1.50g ml 50ml 75ml 25ml B 500nm 530nm
13 c. "Tbcolor Merck" 3 II 0.8% I I II 10 II 10 2% 3%
14 impression film Dienes Dienes 2.5g 1.25g 2.5g 0.25g 100ml 10g Dienes 10g 10g 20g 0.05g 100ml
15 ,18: Davis, J. A.,et al. : Chemical mechanism of the gram stain and synthesis of new electron opaque marker for electron microscopy which replaces the iodine mordant of the stain.j.bacteriol.,156, , ,31, ,1982 MERCK,,1993,
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