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1 * * Fischer 344 Elastica-Masson trichrome NIH Image K + phenylephrine carbachol QOL quality of life

2 Fischer 344 camp, Elastica-Masson trichrome Photoshop Adobe

3 Louis, MO, USA) atropine sulfate mono- O2CO2 hydrate, potassium chloride Krebs-Henseleit ml organ bath 7T mmnacl, KCl, NaHCO, MgSO 4, KH 2 PO 4, glucose, organ bath g Welch's -test KCl mm KCl K + K + Krebs-Henseleit NaCl, KCl mm Elastica-Masson trichrome carbachol propranolol 10-6 M n phenylephrine 10-8 M 10-4 M phenylephrine prazosin 10-7 M n phenylephrine K + prazosin 10-7 M propranolol 10-6 MK + carbachol M M carbamylcholine chloride, phenylephrine ver.5.0 NIH Image ver.1.63 hydrochloride, prazosin hydrochloride, propranolol hydrochloride Sigma chemical Co. (St. ascorbic acid, CaCl 2, ph Student's -test P atropine 10-7 Mn n n n n g /mm 2 n g /mm 2 n g / mm 2 n g / mm 2 n K +

4 會田 今野 石幡 片野 図2 ラット膀胱体部および三角部における 筋組織 線維組織の割合 縦軸 組織面積の割合 若齢ラット 老齢ラット N.S. not significant 内は実験例数 値は 平均値±標準誤差で表している 図3 ラット膀胱体部および三角部における 高濃度 K+ による最大収縮反応 縦軸 収縮張力 若齢ラット 老齢ラット N.S. not significant 内は実験例数 値は 平均値±標準誤差で表している 52

5 phenylephrine K + phenylephrine phenylephrine prazosin prazosin10-7 M prazosin prazosin10-7 M *P. vs phenylephrine carbachol K + phenylephrine n n P 6),7) EC M 10-6 M phenylephrine 1 prazosin carbachol n n EC M, 10-7 M 4)

6 carbachol K + carbachol carbachol 8),9),10) 12) Elastica- KCl Masson trichrome

7 Ca 2+ Ca 2+ KCl KCl mm K + Ca A K + phenylephrine carbachol carbachol EC50 phenylephrine Ordway 13) Fischer 344 Ordway 8) Fischer 344 phenylephrine, norepinephrine, clonidine acetylcholine bethanechol oxotremorine Suzuki 10) EC50 Fischer 344 phenylephrine Fischer ) 1 14) phenylephrine 1 prazosin 1 KCl 1 1 A M 15),16) Suzuki 10) 1 A EC50 2),3)

8 Nishimoto T, Latifpour J, Wheeler MA, Yoshida M, Weiss RM: Age-dependent alterations in -adrenergic responsiveness of rat detrusor smooth muscle. J Urol 1995; 153: Ordway GA, Esbenshade TA, Kolta MG, Gerald MC, Wallace LJ: Effect of age on cholinergic muscarinic responsiveness and receptors in the rat urinary bladder. J Urol 1986; 136: Kolta MG, Wallace LJ, Gerald MC: Agerelated changes in sensitivity of rat urinary bladder to autonomic agents. Mech Ageing Dev 1984; 27: Suzuki Y, Moriyama N, Kanada A, Okaya Y, Kawabe K, Aisaka K: The role of 1Ladrenoceptor in rat urinary bladder: Comparison between young adult and aged rats. Life Sci 1999; 65: Ordway GA, Kolta MG, Gerald MC, Wallace LJ: Age-related change in -adrenergic responsiveness of the urinary bladder of the rat is regionally specific. Neuropharmacol 1986; 25: Andersson KE: The pharmacology of lower urinary tract smooth muscles and penile erectile tissue. Pharmacol Rev 1993; 45: Marshall I, Burt RP, Chapple CR: Noradrenaline contractions of human prostate mediated by 1A-( 1C-) adrenoceptor subtype. Br J Pharmacol 1995; 115: Nasu K, Miriyama N, Fukusawa R: Quantification and distribution of 1-adrenoceptor subtype mrnas in human proximal urethra. Br J Pharmacol 1998; 123: Pagala MK, Tetsoti L, Nagpal D, Wise GJ: Aging effects on contractility of longitudinal and circular detrusor and trigone of rat bladder. J Urol 2001; 166:

9 Yamagata Med J (1) Tomomi Aita, Kinuko Konno, Akira Ishihata*, Yumi Katano Course of Clinical Nursing, *Course of Organ Functions and Controls, Yamagata University School of Medicine, Yamagata, Japan ABSTRACT The urinary bladder dysfunction increases with aging. We previously reported that -adrenoceptors-mediated contraction increases in aged trigone. To further elucidate the mechanisms of age-related urinary bladder dysfunction, we studied the contractile response to phenylephrine and carbachol, in addition to histological examination. Urinary bladders from young (2-3 months) or aged (27 months) male Fischer 344 rats were isolated and the developed tension was recorded. In Elastica- Masson trichrome staining, smooth muscle and collagen were assessed quantitatively using NIH Image. The percentage of smooth muscle and collagen area was not different between young and aged rats. High K + -induced contractions were not significantly changed by aging. Phenylephrine elicited greater contractions in the aged body than those in young rats. In contrast, carbachol-induced contraction in trigone were not significantly changed by aging. It has been reported that relaxing response mediated by -adrenoceptors was decreased in the aged body. These results suggest that the facilitation of contractile response mediated by -adrenoceptors in both body and trigone and the decrease in relaxation to -adrenoceptors in body may be a cause of dysfunction in the aged urinary bladder. Key words : bladder, smooth muscle, aging, -adrenoceptors

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