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1 MDA MDA S A Effect of Cinnamic Acid on Physiological Characteristics of Tomato Seedlings and Alleviation by Carbonized Maize Cob Application LI Liang-liang LI Tian-lai ZHANG En-ping WU Zheng-chao ZANG Jian CHEN Bin LIU Wen-e XI Lian-min Shenyang Agricultural University Shenyang China Abstract We examined the effects of cinnamic acid on the growth of tomato seedlings and its alleviation of applied carbonized maize cob was shown. The tomato seedlings were transplanted in the hydroponic system with perlite as substrate and the biomass photosynthesis ultrastructure of root and MDA were investigated. The results showed that cinnamic acid inhibited the biomass photosynthesis and chlorophyll contents of tomato seedlings. Deformation of the ultrastructures of root was observed and the MDA content was increased by the treatment of high content cinnamic acid. But the inhabitations were alleviated by applied carbonized maize cob. Carbonized corn cob can be used to prevent the plants from monocropping obstacles. Key words Cinnamic acid Tomato Autotoxicity Photosynthesis Ultrastructure MDA Carbonized maize cob 6 7 Yu Yu BAD07B

2 RH % 50% ~ 70% 6 Pn Tr Arnon Lycopersicon esculentum Mill C D 20 d B 2 mm 0. 1 mol /L ph % 1. 5 h Epon-812 LKB-5 JEM-1200X 10 cm 10 cm 1. 4 CK 1 /2 Hoagland Excel A mmol /L 1 /2 SPSS Hoagland B 2. 5 mmol /L 1 /2 Hoagland C 2% mmol /L 1 /2 Hoagland D 2% mmol /L 1 /2 Hoagland ml 150 ml Gs CO 2 Ci 1 A 1. 3 B 20 d 40% ~ 50% 36% a b 23% 36% 20 d Li-6400 Treatment Tab. 1 1 Effect of different disposal on biomass and chlorophyll content of tomato seedlings /g /g /g root aboveground leaf /cm Root length a / mg /g Chlorophyll a b / mg /g Chlorophyll b CK ± 0. 54a ± 0. 44a ± 0. 75a ± 0. 92a ± 0. 04a ± 0. 05a A ± 0. 32a ± 0. 43b ± 0. 68a ± 1. 42a ± 0. 42a ± 0. 05a B ± 0. 25c ± 0. 59c ± 0. 25c ± 1. 95c ± 0. 21b ± 0. 04b C ± 0. 21b ± 0. 39b ± 0. 34b ± 1. 54b ± 0. 19ab ± 0. 06ab D ± 0. 42b ± 0. 68b ± 1. 36b ± 1. 87b ± 0. 24ab ± 0. 08ab P < Note Different letters followed means in a column stand for significant difference at leve1. The same as Tab. 2. C D 2. 2 B 2 A B

3 5 141 CO 2 B 3 D C Treatment Tab. 2 2 / μmol / m 2 s Photosynthesis rate Effect of different disposal on photosynthesis of tomato seedlings / mmol / m 2 s Stomatal conductance CO 2 / μl /L Intercellular CO 2 concentration / mmol / m 2 s Transpiration rate CK ± 0. 42b ± a 322 ± 21a ± 0. 13a A ± 0. 42a ± a 337 ± 15a ± 0. 15a B ± 0. 37c ± b 285 ± 12ab ± 0. 11b C ± 0. 24b ± ab 297 ± 19ab ± 0. 10ab D ± 0. 49b ± ab 316 ± 18a ± 0. 14ab Fig MDA Effect of different disposal on MDA of tomato seedlings 1 MDA B MDA 20 MDA 2. 5 MDA 1. 8 D C MDA B MDA 2. 4 C 2 1. CK 10K 2. B 10K 3. C 10K 4. D 10K N. P. V. B. 1. CK 10K 2. B treatment 10K 3. C treatment 10K 4. D treatment 10K N. Cell nucleus P. Plastid V. Vacuole B. Cell wall. 2 CK B C D Fig. 2 Ultrastructure of root tip of tomato C seedling CK B C and D treatment D

4 Cruz R Ayala C G Anaya A L. Allelochemical stress produced by the aqueous leachates of Callicarpa acuminata effect on roots of beans maize and tomato J. Physiologia Piantarum Amarjeet B Mohini S Sangeeta S. Allelopathic effects of MDA Parthenium hysterophorus on the chlorophyll nitrogen MDA protein and ascorbic acid content of Lantana camara J. 19 Allelopathy Journal MDA 3 Yu J Q Matsui Y. Effects of root exudates of cucumber MDA and allelochemicals on ion uptake by cucumber seedling J. Journal of Chemical Ecology J O J O Polymenakou P N Stephanou E G. Effect of temperature and additional carbon sources on phenol degradation by an indigenous soil J. Pseudomonad. Biodegradation J Yu J Q Lee K S Matsui Y. Effects of the addition of activate carbon on the growth of tomato pants grown by hydroponics J. Soil science and plant nutrition Arnon D I. Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris J. Plant Physiology Hodges D M Delong J M Forney C F et al. Improvig the thiobarbituric acid-reactive-substance assay for estimating lipid peroxidation in plant tissues containing antho-

5 5 143 cyanin and other interfering compounds J. Planta Sannigrahi A K Chakrabortty S. Allelopathic effects of weeds on germination and seedling growth of tomato J. Allelopathy Journal Hiradate S Morita S Furubayashi A et al. Plant growth inhibition by cis-cinnamoyl glucosides and cis-cinnamic acid J. Journal of Chemical Ecology Inderjit Rawat D S Foy C L. Multifaceted approach to determine rice straw phytotoxicity J. Canada Journal of Botany Einhelling F A. The science of allelopathy M. New York J. J Calera M R Anaya A L Gavilanes-Ruiz M. Effect of a phytotoxic resin glycoside on the activity of the H '-AT- Pase from plasma membrane J. Journal of Chemical E- cology 1995b Misako K Shimizu S. Chlorophyll metabolism in higher plants. Ⅵ. Involvement of peroxidase in chlorophyll degradation J. Plant Cell Physiology Cruz Ortega R Alvarez Anorve M Romero Romero M T et al. Growth and oxidative damage effects of Sicyos Deppei weed on tomato J. Allelopathy Journal J J 櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂 CN /S ISSN X http / /www. chinaseedqks. cn chinaseedqks@ sina. com chinaseedqks@ 163. com

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