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1 Studies on the Antigens Expressed on Trophoblasts Related to MLR-blocking Antibodies Shigenori GOTO Department of Obstetrics and Gynecology, Niigata University School of Medicine (Director: Prof. Shoshichi TAKEUCHI) The purpose of this study is to investigate the antigenic nature of trophoblasts and its relation to mixed lymphocyte reaction-blocking antibodies (MLR-BAbs). Mixed culture of lymphocytes and syncytiotropho blasts plasma membrane (STPM) (MLSC) and absorption study of MLR-blocking effect with STPM were perfomed in the present experiments. Lymphocytes were obviously stimulated in the 9 MLSCs. And like MLR, this stimulation was specifically inhibited by corresponding mothers' sera, suggesting that lymphocytes were stimulated in MLSCs by alloantigens expressed on STPM. Nineteen sera were obtained from normal pregnant women and habitual aborters after immunotherapy. And these sera were absorbed with STPM from respective mothers' placenta (autologous STPM) and unrelated mothers' (allogeneic STPM) as a control. The HLA types of cord blood lymphocytes corresponding to these STPM were then determined. The MLR-blocking effect of every serum was removed by autologous STPM absorption, nevertheless it was removed or unaffected by allogeneic one. Comparison of paternal stimulator and cord blood lymphocytes HLA-DR types showed that some of positive absorptions with allogeneic STPM could be explained by coincidence of HLA-DR types. Reprint requests to: Shigenori GOTO, Department of Obstetrics and Gynecology, Niigata University School of Medicine, Niigata City, 951 JAPAN.

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semen quality or those without WBC in semen. In the patients with azoospermia and normal FSH levels (normogonadotropic azzospermia), the antibody (IgG

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