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1 , 2016, ｨｰ06ｨｬ 51, 01 3, : : doi: /agrobiology rus (Triticum aestivum L.) , , , , , ﾁ ｨｰ0208ｨ 0404 ｪ ｨ 0605ｨｹ03ｨ ｨｮ0707ｨ ｨｬ02ｨｪｨｰ0609, ｨｺｨ ｨｰｨ 05ｨｨ04ｨｨ08ｨｮ06ｨｴｨｨ01 08ｨ 09ｨｴ ｨｪｨｨ ｨｪ060500ｨｨ08ｨｪ ｨｦ. 02ｨ 04 ﾂｨｪ06 ｨｨ01 08ｨ ｨｰ ｨｪｨ ﾂ02ｨｰ ｨｰｨｨ07ｨ : ｨｨｨｪ0509ｨｰ0208ｨ 0404 (ｨｪｨ ｨｨｨ ﾂｨ 09ｨｰ06 ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ08ｨｮ06ｨｰ ｨｨ ｨｰ08ｨ 01ｨｨ02ｨｨ06ｨｪｨｪ06ｨｬ ｨｺｨｰ ｨｰｨｨ ﾂ0209ｨｺ06ｨｬ ｨ ｨｪｨ 05ｨｨ0402), ｨ 0202ｨｰｨｨ050509ｨｰ0208ｨ 0404, ｨ 08ｨｨ050509ｨｰ0208ｨ 0404 ｨｨ ｨｺｨ 08ｨ 06ｨｺ09ｨｨ050509ｨｰ0208ｨ ｨｰ0208ｨ ｨ 09ｨｰ02ｨｪｨｨｨｦ ｨｺｨ ｨｰｨ 05ｨｨ04ｨｨ08ｨｮ06ｨｰ ｨ ｨｴ02ｨｪｨｨ02 ｨｺｨ 08ｨ 06ｨｺ09ｨｨ05ｨｹｨｪ ｨｨ ｨ ｨｨ06ｨ ｨｺｨｰｨｨ09ｨｪ0402 ｨｺｨｨ090506ｨｰ04 ｨｨ 0907ｨｨ08ｨｰ04, ｨ 05ｨ ｨ 0807 ﾂ02ｨｬｨｮ ｨｨ0008ｨ 06ｨｰ ｨｺ0506 ﾂ0209ｨｮ ｨｹ 0906 ｨｬｨｪ0600ｨｨ01 ｨ ｨｨ ｨｨ ﾂ0209ｨｺｨｨ ｨ ｨｰ09ｨｮｨｰ09ｨｰ09ｨｨ ｨｨ09ｨｰｨ ｨｰｨｨ ﾂ0209ｨｺｨｨ ｨ ｨｨｨｬ060102ｨｦ09ｨｰ09ｨｨｨｦ ｨｨ ｨｺ060106ｨｬｨｨｨｪｨ ｨｪｨｰｨｪ04ｨｦ 01ｨ 08ｨ ｨｺｨｰ0208 ｨｪｨ ｨ ｨｪｨｨ07 ｨｨ ｨｬ 0509ｨｰ0208ｨ ｨ 06ｨｰ ｨｨ01 04ｨｪｨ ﾂｨｨｨｬ04ｨｬｨｨ ｨ 0409ｨｰ ｨｨ ｨｰｨｮ07ｨｪ ｨｨ04ｨｮ ﾂ02ｨｪｨｨ ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺ06ｨｦ ｨ 01ｨ 07ｨｰｨ 02ｨｨｨｨ ｨｺ ｨｨ04ｨｬ02ｨｪ0706ｨｴｨｨｨｬ0907 ｨｮ ｨｨ07ｨｬ 06ｨｺ08ｨｮ03ｨ 06ｨｴ02ｨｦ ｨｰ06ｨｰ ｨｰｨｨ07 ｨｬｨ 08ｨｺ ｨｮ0106ｨ 02ｨｪ ｨｨ ｨｪｨｨ ｨ ｨｺｨｰｨｨ ﾂ0209ｨｺｨｨ01 04ｨ 01ｨ ﾂ ｨｺ02ｨｨｨｨ 09 ｨｺｨ ﾂ0209ｨｰ ｨｰ09ｨ, ｨ ｨｪ ｨｮ09ｨｺ0608ｨｨｨｰｨｹ ｨｨ ｨｮ ｨｰｨｨｨｰｨｹ ｨｰｨ 0608ｨ ｨｺ02ｨｨ06ｨｪｨｪ06 04ｨｪｨ ﾂｨｨｨｬ ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ ｨｹ06 ｨｪｨ 09ｨｰ0607ｨｴ02ｨｦ 08ｨ ｨ 06ｨｰ04 ｨ 0405ｨ ｨｪｨｺｨ ｨｨ ｨｬ02ｨｪｨｰｨｪ ｨｨ ｨｰ0208ｨ 04, ｨｪｨｪ0401 ｨｨ ｨｬ07ｨｪ, ｨｨ ｨｮ09ｨｰｨ ｨｪ ｨｪｨｨ ｨｨｨｬ060800ｨｨ04ｨｬｨ ｨｬ020301ｨｮ 06ｨ 08ｨ 0402ｨ ｨｬｨｨ ｨｺｨｰｨｨ09ｨｪ ｨｺ02ｨｨ06ｨｪｨｪ ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ ｨｮ 0002ｨｺ09ｨ ｨｨ01ｨｪ06ｨｦ ｨｪｨｨ0204 (Triticum aestivum L.) ｨｰ06ｨｬｨｮ ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺ06ｨｬｨｮ ｨｬｨ 08ｨｺ0208ｨｮ ｨ 05ｨｨ ｨｬ02ｨｪｨ ｨｰ ｨｪｨｨ ｨｺ02ｨｨｨｨ ｨｺ060909ｨｺ ｨｹ09ｨｺ ｨｦ09ｨｰ09ｨ ｨｬ ﾂｨｨｨｪ0609ｨｺｨ 03 (000609ｨｺ060909ｨｺｨ 07 06ｨ 05.) ｪ 0505ｨ ｨｰｨ, 0906ｨ ｨ 09ｨ, 0800ｨ ｨｰｨ, 09ｨｨ04ｨ ( ) ｨｨ ｨ (0604ｨｨｨｬ04ｨｦ); ｨｺ02ｨｨｨｨ ｨｨ04ｨｨ ﾂ0209ｨｺ (080806, ｨ ｨｪｨｺｨｰ-0302ｨｰ0208ｨ ｨｮ0800) ｪ 05ｨｨｨｪｨｨｨｨ ｨｨ ( ), ｨ ｨｰｨ ｨｺ ｨｺ06ｨｬｨ ｨｨｨｪｨ ｨｪｨｰｨｪ0401 ｨｨｨｪｨ ｨｪ ｨｨｨｪｨｨｨｦ ｨｺｨ 08ｨｰｨｨ08ｨｮ06ｨｴ02ｨｦ ｨｮ050702ｨｨｨｨ ITMI ｪ 7, 10, 29, 32, 44, 47, 57, 83, 88, 89 ｨｨ 115 ( ). 0502ｨｬ02ｨｪｨ 08ｨ 04ｨｬｨ ｨ 05ｨｨ 09 00ｨ ｨｦ 09ｨｰｨｮ07ｨｺ02, ｨｬｨｮｨｺｨｮ ｨｨ09ｨ 05ｨｨ ﾂ ｨｨｨｰ ｨｺ09ｨｰ08ｨ ｨｺ02ｨｨｨｨ ｨｬ02ｨｪｨｰ ｨ 08ｨ ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ ｨｨ 06ｨｰｨ ｨｨ08ｨ 05ｨｨ ｨｪｨ ｨ 0106 ﾂｨｪｨｮ06 03ｨｨ01ｨｺ0609ｨｰｨｹ, 04ｨ ｨｰ02ｨｬ 0609ｨｮｨｴ0209ｨｰ ｨｨ ｨｰｨｨｨｺｨ 05ｨｹｨｪ04ｨｦ ｨｪｨ ｨｰｨｨ09ｨｪ04ｨｦ ｨｺｨｰ ｨｺｨ ﾂ0209ｨｰ ｨ ｨｴ ｨｨ ｨｹ040609ｨ 05ｨｨ 8 % ｨｨｨ ｨｺ08ｨｨ05ｨ ｨｬｨｨ01ｨｪ04ｨｦ ｨｹ, 09 ｨｺｨ ﾂ0209ｨｰ0902 ｨｺ06ｨｪ0202ｨｪｨｰ08ｨｨ08ｨｮ06ｨｴ ｪ 4 % ｨｨｨ ｨｺ08ｨｨ05ｨ ｨｬｨｨ01ｨｪ04ｨｦ ｨｹ ｨｺｨｮ050708ｨｪ04ｨｬｨｨ ｨｬｨ 08ｨｺ0208ｨ ｨｬｨｨ 0905ｨｮ03ｨｨ05ｨｨ 06ｨｺ08ｨ 0302ｨｪｨｪ ｨｰｨ ｨｪ01ｨ 08ｨｰ04 Page Ruler Prestained Protein Ladder (00Thermo Scientific03, 09ｨｨｨｰ09ｨ ) ｨｺ06ｨｪ ﾂｨ ｨｪｨｨｨｨ ｨｺｨｰ ｨ ｨｹ 06ｨ 08ｨ ｨ ｨ ｨｰ0409ｨ 05ｨｨ 0802ｨ ｨｺｨｰｨｨ0906ｨｬ ｨｪｨ ｨｪ ｨｨ00ｨｨ ﾂ0209ｨｺｨｮ ｨｰ0208ｨ 04ｨｮ. 05ｨｺｨ ｨｪｨｨ080609ｨ 05ｨｨ ｨｮ ﾂ02ｨｪｨｪ ｨｺｨｰ ｨ ｨｬｨｬ04, ｨｪｨｨ09ｨ 05ｨｨ ｨｨｨｪ01ｨｨ09ｨｨ01ｨｮｨ 05ｨｹｨｪ04ｨｦ ｨｺｨｰ ｨｰｨｨ ﾂ0209ｨｺｨｨｨｦ ｨｨ05ｨｹ ｨｺｨ ｨ 08ｨ 0402ｨ, 08ｨ 0909 ﾂｨｨｨｰ0409ｨ 05ｨｨ 0002ｨｰ ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ ｨｨ ｨｨ ｨｨ ｨｰ0208ｨ 04ｨｪ04ｨｦ ｨｺ06ｨｬ070502ｨｺ09 09 ｨｨ ｨ ｨｪｨｪ ｨｬ02ｨｪｨ ｨｪｨｨ0204 ｨ ｨｰｨ ｨｪ ｨｬｨ ｨｬｨｨ ｨｰ ｨ ｨｰｨ, 0906ｨ ｨ 09ｨ, 0800ｨ ｨｰｨ, 09ｨｨ04ｨ ｨｨ ｨ ｨｨ05ｨｨ 06ｨｰ ｨｨ ｨｬ 09 08ｨ 04ｨｪ06ｨｦ ｨｺｨｰ ｨｰｨｨ ﾂ0209ｨｺ06ｨｦ ｨｨ03ｨｪ0609ｨｰｨｹ06, ｨｮ ｨ ｨｪｨ 08ｨｮ0302ｨｪ06 7, ｪ 8, ｨｮ 05ｨｨｨｪｨｨｨｦ ITMI ｪ 06ｨｰ ｨｨ ｨｬ 0509ｨｰ0208ｨ ｨ 08ｨ ｨ ｨ 08ｨ ｨｺｨｰ0208ｨｪ06 ｨｪｨ 05ｨｨ ﾂｨｨ02 ｨｨ ｨｬ 0509ｨｰ0208ｨ 04 Est-8, Est-9 ｨｨ Est ｨｰ ｨｪｨｪ0609ｨｰｨｹ 06ｨｰｨｬ02 ﾂｨ 05ｨｨ ｨｰ0605ｨｹｨｺ ｨｺｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ ｨｨ ｨｺ0605ｨｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ ｨｰｨ 09ｨｮ 0509ｨｰ0208ｨ ｨ 0605ｨｹ0302ｨｦ ｨｬ060502ｨｺｨｮ050708ｨｪ06ｨｦ ｨｬｨ ｨｦ ｪ Est-1, Est-2, Est-3, Est-4, Est-5, Est-6 ｨｨ Est-7. 08ｨ ｨｦ ｨｨ ｨｰ0609 ｨｨ 05ｨｨｨｪｨｨｨｦ 06ｨ 05ｨ 01ｨ 05 06ｨｰ05ｨｨ ﾂｨｪ04ｨｬ 06ｨｰ 0108ｨｮ00ｨｨ01 06ｨ 08ｨ ｨｪ06ｨｰｨｨ0706ｨｬ ｨｪ ｨｰ ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ (H) 06ｨ 08ｨ ｨｪｨｪ04ｨｬ 0506ｨｺｨｮ09ｨ ｨｬ, ｨｺ0601ｨｨ08ｨｮ06ｨｴｨｨｨｬ ｨｨ ｨｬ ｨｰ0208ｨ 04, ｨｰｨ 09ｨｨ05ｨ 0,924; 01ｨｨ ｨｨ ｨｰ ｨｨ0006ｨｰｨｪ0609ｨｰｨｨ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ ｨｪｨ ｨ 0608ｨ 06ｨ 08ｨ Var(H) = 0, ｨｮ05ｨｹｨｰｨ ｨｰ02 ｨｨ ｨ ｨｪｨｨｨｦ ｨｪ04 ｨｪｨ ｨｨｨ ｨｺｨｰｨｨ09ｨｪ ｨｺ02ｨｨ06ｨｪｨｪ ｨｨｨｰ0205ｨｹ09ｨｺｨｨ ｨｬ04 ｪ ｨｰｨ 0505ｨ ｨｰｨ, ｨ ｨｨ 05ｨｨｨｪｨｨｨｨ , , ITMI7, ITMI44, ITMI83 ｨｨ ITMI ｨ 05ｨｨ ﾂｨｨ02 10 ｨｨ ｨｬ ｨ 02ｨｰ 0509ｨｰ0208ｨ ｨｺ09ｨ ｨｨ01ｨｪ06ｨｦ ｨｪｨｨ0204 ｨｮ0106ｨ ｨｪ04ｨｬ ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺｨｨｨｬ ｨｬｨ 08ｨｺ020806ｨｬ ｨｨ ｨｰ ｨｨｨｰｨｹ ｨｺｨ ｨｺ 00ｨｨ04ｨｨ ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺｨｨ02, ｨｰｨ ｨｺ ｨｨ 0002ｨｪ02ｨｰｨｨｨｺ ｨｺ02ｨｨ06ｨｪｨｪ0402 ｨｨ ｨ ｨｪｨｨ07 06ｨ 08ｨ ｨｺ09ｨ ｨｨ01ｨｪ06ｨｦ ｨｬ0700ｨｺ06ｨｦ ｨｪｨｨ ﾂ ｨ : 0002ｨｺ09ｨ ｨｨ01ｨｪｨ 07 ｨｬ0700ｨｺｨ ｨｪｨｨ02ｨ, ｨｬ02ｨｪｨ, ｨｨ ｨｬ02ｨｪｨｰｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ04, 0509ｨｰ0208ｨ ｨ 0909ｨｨ ﾂ0209ｨｺｨ ｨｪ06ｨｰｨｨ07ｨｨ ﾂ0209ｨｺｨ ｨｪｨｺｨ ｨｺ02ｨｨ06ｨｪｨｪ06 04ｨｪｨ ﾂｨｨｨｬ ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ 09ｨｰｨ ｨｪ0609ｨｨｨｰ ｨｬ02ｨｪ ｨｪ06ｨｦ 09 ｨｮ090205ｨｨ ﾂ02ｨｪｨｨ02ｨｬ ﾂｨｨ0905ｨ ｨｺ ｨｺ02ｨｨｨｦ 0002ｨｪ02ｨｰｨｨ ﾂ0209ｨｺｨｨ ｨｮ ｨ 09ｨｰ02ｨｪｨｨｨｦ, ｨｺ0605ｨｹｨｺｨｮ ｨｰ0802ｨ ｨｮ02ｨｰ ｨｬ02ｨｪｨｪ0401 ｨｨ 0102ｨｪ0203ｨｪ ｨ ｨｰ08ｨ ｨｰ, ｨ ｨｮ ﾂ02ｨｪｨｪ ｨｪ06ｨｰｨｨ07ｨ ｨｮ05ｨｹｨｰｨ ｨｰ04 09ｨ 08ｨｹｨｨ08ｨｮ06ｨｰ 0708ｨｨ ｨ ｨｴｨｨ09ｨ ｨｪｨｨｨｨ 09 08ｨ 0405ｨｨ ﾂｨｪ0401 ｨｮ ｨｨ ｨｪｨ 05ｨｨ04 ｨｪｨ 0609ｨｪ0609ｨ ｨｪｨｨｨｨ ｨｨｨｬ060800ｨｪ ｨｺｨｰ ｨｰｨｨ ﾂ0209ｨｺ ｨｺｨｰ08ｨ ｨ 0205ｨｺ ｨｨ ｨｬ02ｨｪｨｰｨｪ0401 ｨｬｨ 08ｨｺ ｪ 05ｨｰ06 ｨ ｨｦ , ｨｺ06ｨｰ060804ｨｦ 09 ｨｮ ｨｬ ｨｬ060302ｨｰ 0708ｨｨｨｬ02ｨｪ07ｨｰｨｹ ｨｺ ｨｪｨｺｨｨ 0002ｨｪ02ｨｰｨｨ ﾂ0209ｨｺｨｨ ｨｮ ｨ 09ｨｰ02ｨｪｨｨｨｦ, ｨｮ09ｨｰｨ ｨｪ ｨｪｨｨ07 00ｨｨ ｨｪ02ｨｰｨｨ- ﾂ0209ｨｺｨｨ01 06ｨｰｨｪ060302ｨｪｨｨｨｦ ｨｬ020301ｨｮ 08ｨ 04ｨｪ04ｨｬｨｨ 09ｨｨ01ｨ ｨｬｨｨ ｨｨ ｨｪｨｨ ｨｦ00ｨ 0002ｨｪ0609 ｨｨ05ｨｨ ｨｨｨｪ ｨｪ02ｨｰｨｨｨｺ ｨｨ06ｨｪｨｪ ｨｪｨ ｨｮ050702ｨｨ06ｨｪｨｪ06ｨｬ ｨｮ080609ｨｪ02 (1-5). 327

2 130609ｨｰ0208ｨ 0404 ｪ ｨ 0605ｨｹ03ｨ ｨｮ0707ｨ ｨｬ02ｨｪｨｰ0609 (ｨｪ02 ｨｬ02ｨｪ ), ｨｺｨ ｨｰｨ 05ｨｨ04ｨｨ08ｨｮ06ｨｴｨｨ01 08ｨ 09ｨｴ ｨｪｨｨ ｨｪ060500ｨｨ08ｨｪ ｨｦ ｨｨ0806ｨｺ06ｨｬ 09ｨｬ ｨｺ 0509ｨｰ0208ｨ 04ｨ ｨｬ 06ｨｰｨｪ060907ｨｰ ｨｨ07ｨ 0404, ｨ ｨｰｨ 0404, 09ｨｮ05ｨｹ00ｨ ｨｰｨ 0404 ｨｨ 0906ｨ 09ｨｰ0902ｨｪｨｪ ｨｰ0208ｨ ｨｪｨｨ02 09ｨｺ0506 ﾂｨ 06ｨｰ ｨ 07 ｨｬｨｪ ﾂｨｨ090502ｨｪｨｪ ｨｨ00ｨｨ ﾂ0209ｨｺｨｨ ｨｬ02ｨｪｨｰ04: ｨｨｨｪ0509ｨｰ0208ｨ 04ｨｮ, ｨｨ0505ｨ 04ｨｮ, ｨｰｨ ｨｪｨｪｨ 04ｨｮ, 0702ｨｺｨｰｨ 04ｨｮ ｨｨ ｨ 04 ﾂｨｪ ｨｰ0208ｨ ｨ ｨｰ ｨｪｨ ﾂ02ｨｰ ｨｰｨｨ07ｨ : ｨｨｨｪ0509ｨｰ0208ｨ ﾂｨｨｨｰｨ 06ｨｰ 04ｨ ｨｪｨｪ ｨｮｨ 09ｨｰ08ｨ ｨｰ04 (0500ｨｨ ｨｨｨｪｨ 09 ｨｮｨｺ09ｨｮ09ｨｪ06ｨｦ, ｨｨ06ｨｪ060906ｨｦ ｨｨ05ｨｨ ｨｬｨ ｨｪ06ｨｦ ｨｺｨｨ090506ｨｰｨ ｨｬｨｨ), 05ｨｰ06ｨｰ ｨｰｨｨ ｨｰ0208ｨ 04 ｨｪｨ ｨｨｨ ﾂｨ 09ｨｰ06 ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ08ｨｮ02ｨｰ ｨｨ ｨｰ08ｨ 01ｨｨ02ｨｨ06ｨｪｨｪ06ｨｬ ｨｺｨｰ ｨｰｨｨ ﾂ0209ｨｺ06ｨｬ ｨ ｨｪｨ 05ｨｨ0402; ｨ 0202ｨｰｨｨ050509ｨｰ0208ｨ 0404 ｨｨ ｨｹ04ｨｮ06ｨｰ 09 ｨｺｨ ﾂ0209ｨｰ ｨｮｨ 09ｨｰ08ｨ ｨｰ0609 ｨ 05ｨｨ00ｨ ｨｰｨｨ ﾂ0209ｨｺｨｨ02 ｨｮ , 09 ﾂｨ 09ｨｰｨｪ0609ｨｰｨｨ ｨｮｨｺ09ｨｮ09ｨｪｨｮ06 ｨｺｨｨ090506ｨｰｨｮ; ｨ 08ｨｨ050509ｨｰ0208ｨ 0404 ｨｨｨｪ00ｨｨｨ ｨｨ08ｨｮ06ｨｰ ｨｪ04ｨｬｨｨ ｨｺ06ｨｬ0706ｨｪ02ｨｪｨｰｨ ｨｬｨｨ, 09ｨｮｨ 09ｨｰ08ｨ ｨｰｨ ｨｬｨｨ ｨｪｨｨ ｨｮ03ｨ ｨｰ ｨ 0806ｨｬｨ ｨｰｨｨ ﾂ0209ｨｺｨｨ02 ｨｮ ; ｨｺｨ 08ｨ 06ｨｺ09ｨｨ050509ｨｰ0208ｨ ﾂｨｨｨｰｨ 06ｨｰ 0500ｨｨ0804 ｨ 05ｨｨ00ｨ ｨｰｨｨ ﾂ0209ｨｺｨｨ01 (010609ｨｰｨ ｨｰ06 ﾂｨｪ ｨｨｨｪｨｪ040103) ｨｨｨｪ02ｨｪｨｨｨｦ, ｨｨｨｪ00ｨｨｨ ｨｨ08ｨｮ06ｨｰ0907, ｨｺｨ ｨｺ ｨｨ ｨｨｨｪ0509ｨｰ0208ｨ 0404, ｨ ｨｪｨｨ ﾂ0209ｨｺｨｨｨｬｨｨ 0902ｨｴ0209ｨｰ09ｨ ｨｬｨｨ (6-9). 0609ｨｰ0208ｨ ｨ 09ｨｰ02ｨｪｨｨｨｦ (EC X) ｪ 05ｨｰ06 08ｨ 04ｨｪ0609ｨｨ01ｨｪ0609ｨｰｨｹ 0509ｨｰ0208ｨ 04, 0708ｨｨ09ｨｮｨｰ09ｨｰ09ｨｮ06ｨｴｨｨ01 ｨｮ 0906ｨｨ, ｨｪｨｨ0204, , 08ｨｨ09ｨ ｨｨ 0108ｨｮ00ｨｨ01 09ｨｨ (6, 10, 11). 02ｨｪｨｨ ｨｺｨ ｨｰｨ 05ｨｨ04ｨｨ08ｨｮ06ｨｰ ｨ ｨｴ02ｨｪｨｨ02 ｨｺｨ 08ｨ 06ｨｺ09ｨｨ05ｨｹｨｪ ｨｨ ｨ ｨｨ06ｨ ｨｺｨｰｨｨ09ｨｪ0402 ｨｺｨｨ090506ｨｰ04 ｨｨ 0907ｨｨ08ｨｰ04, ｨ 05ｨ ｨ 0807 ﾂ02ｨｬｨｮ ｨｨ0008ｨ 06ｨｰ ｨｺ0506 ﾂ0209ｨｮ ｨｹ 0906 ｨｬｨｪ0600ｨｨ01 ｨ ｨｨ ｨｨ ﾂ0209ｨｺｨｨ ｨ 01, ｨｪｨ 0708ｨｨｨｬ ｨ ｨｺｨｰｨｨ09ｨ 02ｨｨｨｨ 09ｨｨ00ｨｪｨ 05ｨｹｨｪ0401 ｨｬ060502ｨｺｨｮ05 (12) ｨｨ ｨｮ050702ｨｨｨｨ ｨ ｨｨ06ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｨ 05ｨｪ ｨｪｨｪ ｨｮｨｺｨｰ0609 (13), 09ｨｺ0506 ﾂｨ 07 00ｨｨ ｨｨｨｰｨｨ ﾂ0209ｨｺ ｨ 09ｨｴ ｨｪｨｨ ｨｨ08ｨｪ ｨｦ ｨｰｨｨ02ｨｨ ｨｨ 0108ｨｮ00ｨｨ01 ｨｺ0902ｨｪ06ｨ ｨｨ06ｨｰｨｨｨｺ0609 (6). 06ｨ ｨｺ, ｨ ｨｨ02ｨｨ0104 ｨｰｨｨｨ ｨｨ08, 01ｨｨｨｺ ｨｬ02ｨｰｨｨ05 ｨｨ ｨ 0806ｨｬ06ｨｺ09ｨｨｨｪｨｨ0506ｨｺｨｰｨ ｨｪ06ｨ ｨｰ, ｨ ｨｰｨ ｨｺ ｨｮｨｪ00ｨｨ02ｨｨ01 ｨ ｨｨｨｪｨ 07ｨ ｨｺ08ｨｨ05 ｨｨｨｪｨ ｨｺｨｰｨｨ09ｨｨ08ｨｮ06ｨｰ ｨｦ09ｨｰ09ｨｨ02ｨｬ 0509ｨｰ0208ｨ 04 (6, 14). 0102ｨｺ09ｨ ｨｨ01ｨｪｨ ｨｪｨｨ02ｨ (Triticum aestivum L.) ｪ 0601ｨｨｨｪ ｨｨ ｨ 02ｨｬ ｨｨ010609, ｨｺ06ｨｰ ｨｺ06ｨｬ0706ｨｪ02ｨｪｨｰｨｪ04ｨｦ ｨｰｨ ｨｰ0208ｨ 04 ｨ 0405 ｨｨ04ｨｮ ﾂ02ｨｪ, ｨｪ ｨｪ02ｨｰｨｨ ﾂ0209ｨｺｨｨｨｦ ｨｺ06ｨｪｨｰ080605ｨｹ ｨｨ01 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｨ ｨｨ ｨｪ0609ｨｰｨｹ06 ｨｪ02 ｨｮ09ｨｰｨ ｨｪ ｨｪ. 0207ｨｨ09ｨ ｨｪ04 ﾂ02ｨｰ ｨ 0506ｨｺｨ 0006ｨｬ ｨｨ ﾂｨｪ ｨｺｨｮ090609, ｨｺ06ｨｪｨｰ080605ｨｨ08ｨｮ06ｨｴｨｨ ｨｰ0208ｨ ｨ 0405ｨｨ ﾂｨｪ ｨ ｨｪｨ 01 ｨｨ ｨｰｨｺｨ ｨｪ ｨｪｨｨ ｨｪ ｨｰ0208ｨ 04 ｨｪ ｨｬ07ｨｪ, ｨｺ0608ｨｪ02ｨｦ, 05ｨｨ09ｨｰ ｨ 09ｨｰｨｨｨｪ06ｨｺ ｨｨ ｨｪ ｨｨ09ｨｰｨｹ ｨ 09ｨｰ02ｨｪｨｨｨｦ ｨｺｨ 08ｨｰｨｨ080609ｨ ｨｪ04 ｨｪｨ 3AS, 3BS ｨｨ 3DS ﾂｨ ｨｬ060906ｨｬ (15-17). 0906ｨｺｨｮ ｨｰ0208ｨ 04 05ｨｰｨｨ0605ｨｨ080609ｨ ｨｪｨｪ0401 ｨｺ ｨｰｨｨ0502ｨｦ ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ080609ｨ ｨｪ04 ｨｪｨ 3AL, 3BL, 3DL, 7BS ｨｨ 7DS (18), 05ｨｰｨｨ0605ｨｨ080609ｨ ｨｪｨｪ ｨｨ09ｨｰｨｹ0209 ｪ ｨｪｨ 6AL, 6BL ｨｨ 6DL (18-20), ｨｬ07ｨｪ ｪ ｨｪｨ 0105ｨｨｨｪｨｪ ﾂｨ ｨｬ060906ｨｬ 3AL, 3BL ｨｨ 3DL (20-22). 0609ｨｰ0208ｨ ｨｪｨｨ ｨｰ0902ｨｰ09ｨｰ09ｨｨｨｨ ｨｰ08ｨｮｨｺｨｰｨｮ08ｨｪ06ｨｦ ｨｺ06ｨｬ070604ｨｨ02ｨｨ02ｨｦ ｨ 0405ｨｨ 0607ｨｨ09ｨ ｨｪ04 ｨｺｨ ｨｺ ｨｬ06ｨｪ06ｨｬ ｨｨ05ｨｨ 01ｨｨｨｬ (15, 18, 19). 02ｨｰ09ｨｮｨｰ09ｨｰ09ｨｨ ｨｨ09ｨｰｨ ｨｰｨｨ ﾂ0209ｨｺｨｨ ｨ ｨｨｨｬ060102ｨｦ09ｨｰ09ｨｨｨｦ ｨｨ ｨｺ060106ｨｬｨｨｨｪｨ ｨｪｨｰｨｪ04ｨｦ 01ｨ 08ｨ ｨｺｨｰ0208 ｨｪｨ ｨ ｨｪｨｨ07 ｨｨ ｨｬ 0509ｨｰ0208ｨ ｨ 06ｨｰ ｨｨ01 04ｨｪｨ ﾂｨｨｨｬ04ｨｬｨｨ ｨ 0409ｨｰ ｨｨ ｨｰｨｮ07ｨｪ ｨｨ04ｨｮ ﾂ02ｨｪｨｨ ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺ06ｨｦ ｨ 01ｨ 07ｨｰｨ 02ｨｨｨｨ ｨｺ ｨｨ04ｨｬ02ｨｪ0706ｨｴｨｨｨｬ0907 ｨｮ ｨｨ07ｨｬ 06ｨｺ08ｨｮ03ｨ 06ｨｴ02ｨｦ ｨｰ06ｨｰ ｨｰｨｨ07 ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺｨｨ01 ｨｬｨ 08ｨｺ ｨｮ0106ｨ 02ｨｪ ｨｨ ｨｪｨｨ ｨ ｨｺｨｰｨｨ ﾂ0209ｨｺｨｨ01 04ｨ 01ｨ ﾂ ｨｺ02ｨｨｨｨ 09 ｨｺｨ ﾂ0209ｨｰ ｨｰ09ｨ, ｨ ｨｪ ｨｮ09ｨｺ0608ｨｨｨｰｨｹ ｨｨ ｨｮ ｨｰｨｨｨｰｨｹ ｨｰｨ 0608ｨ ｨｺ02ｨｨ06ｨｪｨｪ06 04ｨｪｨ ﾂｨｨｨｬ ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ. 00 ｨｪｨ 09ｨｰ0607ｨｴ02ｨｦ 08ｨ ｨ 06ｨｰ02 ｨｬ ｨｨ ｨｨ ｨｬ02ｨｪｨｰｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ ｨ 05ｨｨｨｪｨｨｨｦ ｨｨ ｨｰ ｨｦ ｨｨ 0604ｨｨｨｬ06ｨｦ ｨｪｨｨ0204, ﾂｨｰ ｨｨ ｨｨｨｰｨｹ ｨｬ0603ｨｪ ｨｨｨｰ0205ｨｹ09ｨｺｨｨ ｨｬ ｨｮ06ｨｴ ｨｮ ﾂ02ｨｪｨｨ07 ｨｨ ｨｪ ｨｨ 00ｨｨｨ 08ｨｨ01ｨｪ ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ ｨｺ02ｨｨ06ｨｪｨｪ ｨ ｨｬｨｬｨ 01, ｨｪｨ 0708ｨ ｨｪｨｪ0401 ｨｪｨ ｨｪｨｨ02 ｨｮ09ｨｰ06ｨｦ ﾂｨｨ090609ｨｰｨｨ ｨｨ ｨｮｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｨ ｨｮ Triticum aestivum L ｨｹ06 08ｨ ｨ 06ｨｰ04 ｨ 0405ｨ ｨｪｨｺｨ ｨｨ ｨｬ02ｨｪｨｰｨｪ ｨｨ ｨｰ0208ｨ 04, ｨｪｨｪ0401 ｨｨ ｨｬ07ｨｪ, ｨｨ ｨｮ09ｨｰｨ ｨｪ ｨｪｨｨ ｨｨｨｬ060800ｨｨ04ｨｬｨ ｨｬ020301ｨｮ 06ｨ 08ｨ 0402ｨ ｨｬｨｨ ｨｺｨｰｨｨ09ｨｪ ｨｺ02ｨｨ06ｨｪｨｪ ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ ｨｮ 0002ｨｺ09ｨ ｨｨ01ｨｪ06ｨｦ ｨｪｨｨ ｨｰ06ｨｬｨｮ ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺ06ｨｬｨｮ ｨｬｨ 08ｨｺ0208ｨｮ. 0002ｨｰ0601ｨｨｨｺｨ. 00ｨ ｨｰ0208ｨｨｨ 0506ｨｬ ｨｨ ｨ ｨｪｨｨｨｦ 0905ｨｮ03ｨｨ05ｨｨ ｨｬ02ｨｪｨ ｨｰ ｨｪｨｨ0204 (Triticum aestivum L.) ｨｺ02ｨｨｨｨ ｨｺ060909ｨｺ ｨｹ- 328

3 1309ｨｺ ｨｦ09ｨｰ09ｨ ｨｬ ﾂｨｨｨｪ0609ｨｺｨ 03 ｪ 0505ｨ ｨｰｨ, 0906ｨ ｨ 09ｨ, 0800ｨ ｨｰｨ, 09ｨｨ04ｨ ( ) ｨｨ ｨ (0604ｨｨｨｬ04ｨｦ); ｨｺ02ｨｨｨｨ ｨｨ04ｨｨ ﾂ0209ｨｺ (080806) ｪ 05ｨｨｨｪｨｨｨｨ ｨｨ ( ), ｨ ｨｰｨ ｨｺ ｨｺ06ｨｬｨ ｨｨｨｪｨ ｨｪｨｰｨｪ0401 ｨｨｨｪｨ ｨｪ ｨｨｨｪｨｨｨｦ ｨｺｨ 08ｨｰｨｨ08ｨｮ06ｨｴ02ｨｦ ｨｮ050702ｨｨｨｨ ITMI ｪ 7, 10, 29, 32, 44, 47, 57, 83, 88, 89 ｨｨ 115 ( ). 0502ｨｬ02ｨｪｨ ｨｰｨｴｨ ｨｰ0205ｨｹｨｪ06 08ｨ 04ｨｬｨ ｨ 05ｨｨ 09 00ｨ ｨｦ 09ｨｰｨｮ07ｨｺ02, ｨｬｨｮｨｺｨｮ ｨｨ09ｨ 05ｨｨ ﾂ ｨｨｨｰ06 (ｨｬ02ｨｪ ｨｬｨｺｨｬ). 06ｨｺ09ｨｰ08ｨ ｨｺ02ｨｨ ｨｬ02ｨｪｨｰ ｨｨ05ｨｨ 0708ｨｨ ｨｰ02ｨｬ070208ｨ ｨｰｨｮ C 09 ｨｰ02 ﾂ02ｨｪｨｨ02 ｨｪ06 ﾂｨｨ, ｨｰｨｪ060302ｨｪｨｨ02 ｨｬｨｮｨｺｨ :ｨ ｨｮ ｪ 1:2. 00 ｨｺｨ - ﾂ0209ｨｰ0902 ｨ ｨｮ000208ｨ ｨｨ ｨｹ040609ｨ 05ｨｨ 0, ｨｨ l (0801 8,3), 0,001 M MgCl 2, 10 % 0005ｨｨ020208ｨｨｨｪ. 06ｨｺ09ｨｰ08ｨ ｨｺｨｰ ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ 5 ｨｬｨｨｨｪ 0708ｨｨ ｨ /ｨｬｨｨｨｪ ｨｨ 06ｨｰｨ ｨｨ08ｨ 05ｨｨ ｨｪｨ ｨ 0106 ﾂｨｪｨｮ06 03ｨｨ01ｨｺ0609ｨｰｨｹ. 05ｨ ｨｰ02ｨｬ 0609ｨｮｨｴ0209ｨｰ ｨｨ ｨｰｨｨｨｺｨ 05ｨｹｨｪ04ｨｦ ｨｪｨ ｨｰｨｨ09ｨｪ04ｨｦ ｨｺｨｰ (23). 00 ｨｺｨ ﾂ0209ｨｰ ｨ ｨｴ ｨｨ ｨｹ040609ｨ 05ｨｨ 8 % ｨｨｨ ｨｺ08ｨｨ05ｨ ｨｬｨｨ01ｨｪ04ｨｦ ｨｹ, 09 ｨｺｨ ﾂ0209ｨｰ0902 ｨｺ06ｨｪ0202ｨｪｨｰ08ｨｨ08ｨｮ06ｨｴ ｪ 4 % ｨｨｨ ｨｺ08ｨｨ05ｨ ｨｬｨｨ01ｨｪ04ｨｦ ｨｹ ｨｺｨｰ ｨｨ05ｨｨ 09 ｨｺｨ ｨｬ Mini-PROTEAN Tetra System (00Bio-Rad Laboratories, Inc.03, ) 0708ｨｨ 50 ｨｬ08/ ﾂ ｨｨ ｨｰ02ｨｬ070208ｨ ｨｰｨｮ ｨｺｨｮ050708ｨｪ04ｨｬｨｨ ｨｬｨ 08ｨｺ0208ｨ ｨｬｨｨ 0905ｨｮ03ｨｨ05ｨｨ ｨｺ08ｨ 0302ｨｪｨｪ ｨｰｨ ｨｪ01ｨ 08ｨｰ04 Page Ruler Prestained Protein Ladder (00Thermo Scientific03, 09ｨｨｨｰ09ｨ ). 01ｨ 0601ｨｪｨｮ ｨｺｨｮ ｨｪ0609ｨｨ05ｨｨ ｨｬｨｺ05 08ｨ 09ｨｰ090608ｨ ｨ 0205ｨｺｨ (ｨ 0204 ｨｺ08ｨ 09ｨｨｨｰ020502ｨｦ ｨｨ 0108ｨｮ00ｨｨ01 ｨ 0002ｨｪｨｰ0609), ｨｺ06ｨｪ ﾂｨ ｨｪｨｨｨｨ ｨｺｨｰ ｨ ｨｹ 06ｨ 08ｨ ｨ ｨ ｨｰ0409ｨ 05ｨｨ 0802ｨ ｨｺｨｰｨｨ0906ｨｬ ｨｪｨ ｨｪ ｨｨ00ｨｨ ﾂ0209ｨｺｨｮ ｨｰ0208ｨ 04ｨｮ ｨｰ ｨｹ ｨｨ09ｨ 05ｨｨ ｨｨ0006ｨｰ ｨｪｨｪ06ｨｬ 08ｨ 09ｨｰ ｨｺ08ｨ 09ｨｨｨｰ ｨｰ02 ﾂ02ｨｪｨｨ ｨｬｨｨｨｪ ｨｪｨｨ07 ｨｺ0608ｨｨ ﾂｨｪ ｨｪ. 0808ｨ 09ｨｨｨｰ0205ｨｹ ｨｨ ｨｬ 0509ｨｰ0208ｨ ｨｰ0609ｨｨ05ｨｨ ｨｮ06ｨｴｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ: 0, ｨｪｨ 00ｨｰｨｨ05ｨ 0202ｨｰｨ ｨｰｨ (00Sigma-Aldrich03, ｨｦ02ｨ 08ｨｨ07) 08ｨ 09ｨｰ ｨｨ 09 5 ｨｬ05 70 % 05ｨｰｨ ｨｪ0605ｨ, 04ｨ ｨｰ02ｨｬ ｨｬ ｨｪｨｪ06, 0708ｨｨ ｨｬ0203ｨｨ09ｨ ｨｪｨｨｨｨ 0905ｨｨ09ｨ 05ｨｨ ｨｬ05 0, ｨ ｨｰｨｪ ｨ ｨｮ000208ｨ (0801 6,0); 0, ｨｨｨｪ ﾂｨｪ Fast Blue RR (00Aldrich03, ) 08ｨ 09ｨｰ ｨｨ 09 4 ｨｬ ｨ ｨｪ0605ｨ ｨｨ 15 ｨｬ05 0, ｨ ｨｰｨｪ ｨ ｨｮ000208ｨ (0801 6,0). 02ｨ ｨ 08ｨ 09ｨｰ090608ｨ 09ｨｬ0203ｨｨ09ｨ 05ｨｨ (1:1), 00ｨｨ05ｨｹｨｰ080609ｨ 05ｨｨ ﾂ ｨｺ05ｨ 01 ﾂｨ ｨｰ04ｨｦ ｨ ｨｮｨｬｨ 03ｨｪ04ｨｦ 00ｨｨ05ｨｹｨｰ08 ｨｨ 0908ｨ 04ｨｮ 0302 ｨｨ ｨｹ040609ｨ 05ｨｨ ｨｪｨｨ ｨｪ ｨｰ0208ｨ 04ｨｪ06ｨｦ ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ06 (24) ｨｰ ｨｺｨ ｨｺ 0406ｨｪ04 09ｨｰｨ ｨｪ0609ｨｨ05ｨｨ09ｨｹ ｨｰ02ｨｬｨｪ04ｨｬｨｨ, ｨｨ04ｨ 04ｨｰ06ｨｺ ｨｺ08ｨ 09ｨｨｨｰ ｨｮ01ｨ ｨｨ, ｨｬ0409ｨ ｨｹ 7 % ｨｮｨｺ09ｨｮ09ｨｪ06ｨｦ ｨｺｨｨ090506ｨｰ06ｨｦ ｨｮ ﾂ02ｨｪｨｪ ｨｺｨｰ ｨ ｨｬｨｬ04 09ｨｺｨ ｨｪｨｨ080609ｨ 05ｨｨ ｨｪｨ Epson Expression XL (00GE Healthcare03, ). 06ｨｪ01ｨｨ09ｨｨ01ｨｮｨ 05ｨｹｨｪ04ｨｦ ｨｺｨｰ ｨｰｨｨ ﾂ0209ｨｺｨｨｨｦ ｨｨ05ｨｹ ｨｺｨ ｨ 08ｨ 0402ｨ ｨｪｨｨ09ｨ 05ｨｨ ｨｬ06ｨｴｨｹ ｨ ｨｬｨｬ04 Phoretix 1D (00TotalLab, Ltd.03, ｨｨｨｺ06ｨ 08ｨｨｨｰｨ ｨｪｨｨ07). 04ｨ 09 ﾂ02ｨｰ 0002ｨｰ ｨｨ0006ｨｰｨｪ0609ｨｰｨｨ ｨｨ ｨｨ ｨｨｨｨ ｨｨ05ｨｨ ｨｬｨｮ05ｨ ｨｬ, ｨｪｨｪ04ｨｬ 00. Nei (25) ｨｨ ｨｦ08 (26), ｨ ｨ 04ｨｨ08ｨｮ0709ｨｹ ｨｪｨ 0607ｨｨ09ｨ ｨｪｨｪ0401 ｨｪｨ ｨｬｨｨ 08ｨ ｨｪ0202 ｨ ｨｨｨｰｨｬｨ 01 ｨｺ0605ｨｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｦ ｨｪｨｺｨｨ 09ｨｰ020702ｨｪｨｨ ｨｨｨｬ060800ｨｨ04ｨｬｨ (27) ｨｮ05ｨｹｨｰｨ ｨｰ ｨｮ ﾂ02ｨｪｨｨ02 ｨｨ 0607ｨｨ09ｨ ｨｪｨｨ02 05ｨｨｨｪｨｨｨｦ ｨｺｨ 08ｨｰｨｨ08ｨｮ06ｨｴ02ｨｦ ｨｮ050702ｨｨｨｨ ITMI ｨ 0405ｨｨ 0607ｨｮｨ 05ｨｨｨｺ0609ｨ ｨｪ04 ｨｪｨ ｨｬｨｨ 08ｨ ｨｪ0202 (28-30). 0207ｨｨ09ｨ ｨｪｨｨ02 ｨｨ 01ｨ 08ｨ ｨｺｨｰ0208ｨｨ09ｨｰｨｨｨｺｨｨ ｨｰ ｨ ｨｰｨ, 0906ｨ ｨ 09ｨ, 0800ｨ ｨｰｨ, 09ｨｨ04ｨ ｨｨ ｨ 0708ｨｨ ｨｪ ｨｰ0902ｨｰ09ｨｰ09ｨｮ06ｨｴｨｨ01 ｨ ｨ 04ｨ 01 01ｨ ｨｪｨｪ ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ ｨｰ08ｨ ｨｨｨｪｨｨ ｨ 0405ｨ ｨｮ ﾂ02ｨｪｨ ｨｰ0906ｨｬ ｨｨｨｪ01ｨｨ09ｨｨ01ｨｮｨ 05ｨｹｨｪ ｨｰｨ 0608ｨ 09 ｨｺ06ｨｬｨ ｨｨｨｪｨ 02ｨｨｨｨ 09ｨｺ0802ｨｴｨｨ09ｨ ｨｪｨｨ ｨｰ ｨｦ ｨｬ0700ｨｺ06ｨｦ ｨｪｨｨ0204 Asakaze komugi ｨｪｨｨｨｪ0008ｨ 0109ｨｺｨ 07 08ｨ ｨｪｨｪ ｨｨｨｪｨｨ07 09ｨｺ ｨ 07, ｨｪｨｨ04ｨｺ ｨ 07, ｨｮ0609ｨｰｨｨ09ｨｰｨｨ09ｨｰｨ ｨｬｨ ｨｺｨｮ09ｨｰｨ ｨｺｨｰ06ｨｨ01ｨｪｨ ｨｨ09ｨｰ0609ｨ ｨ 09ｨｰｨｨｨｪｨｺｨ 0105ｨｨｨｪｨｪｨ 07 ｨｨ ｨｮ04ｨｺｨ ｨｨ08ｨ ｨｬｨｨ01ｨ 05ｨｹｨｪ04ｨｦ, ｨｰｨｪ0609ｨｰｨｹ ｨｪ0707, 0105ｨｨｨｪｨ ｨｺ ｨ ｨｪ0707, ﾂｨｨ ｨｪ 09 ｨｺ ｨｬｨ ｨ 0909ｨ ｨｪｨ 09 ｨｺ ｨ ｨｨ04ｨｬ02ｨｪ0702ｨｰ ｨｰ ｨｬｨ 0506ｨｦ ｨｪ02ｨｦ, ｨｪ06 ｨｬ0205ｨｺ0602, ｨｮ0105ｨｨｨｪ02ｨｪｨｪ0602, 06ｨｺ08ｨ 09ｨｺｨ ｨｺ08ｨ 09ｨｪｨ ｨｨｨｪｨｨ ｨｮ ﾂｨｨ05ｨｨ ｨｨｨｪ01ｨｨ09ｨｨ01ｨｮｨ 05ｨｹｨｪ04ｨｬ 06ｨｰｨ ｨｬ 09 ｨｺ06ｨｬｨ ｨｨｨｪｨ 02ｨｨｨｨ 09ｨｺ0802ｨｴｨｨ09ｨ ｨｪｨｨ ｨｰ ｨｦ ｨｬ0700ｨｺ06ｨｦ ｨｪｨｨ0204 Asakaze komugi 05 Sonora ｨｨｨｪｨｨ07 09ｨｺ ｨ 07, ｨｮｨｺｨ 0805ｨｨｨｺ0609ｨ 07, ｨｮ0609ｨｰｨｨ09ｨｰｨｨ09ｨｰｨ ｨｬｨ ｨｺｨｮ09ｨｰｨ ｨｪｨ ｨｺ0506ｨｪｨｪｨ ｨｨ09ｨｰ0609ｨ ｨ 09ｨｰｨｨｨｪｨｺｨ ｨｪ ｨｨｨｪ02 ｨｨ 03ｨｨ08ｨｨｨｪ ｨｨ08ｨ ｨｬｨｨ01ｨ 05ｨｹｨｪ04ｨｦ, ｨｰｨｪ0609ｨｰｨｹ ｨｪ0707, 0105ｨｨｨｪｨ ｨｺ ｨ ｨｪ0707, ﾂｨｨ ｨｪ 09 ｨｺ ｨｪ ｨ 0909ｨ ｨｪｨ 09 ｨｺ ｨ ｨｨ04ｨｬ02ｨｪ0702ｨｰ ｨｰ ｨｬｨ 0506ｨｦ ｨｪ02ｨｦ, ｨｪ ｨｪ0202, 06ｨｺ08ｨ 09ｨｺｨ ｨｺ08ｨ 09ｨｪｨ

4 130609ｨｰ0208ｨ 04ｨｪ04ｨｦ ｨｺ06ｨｬ070502ｨｺ09 09 ｨｨ ｨ ｨｪｨｪ ｨｬ02ｨｪｨ ｨｪｨｨ0204 ｨ ｨｰｨ ｨｪ ｨｬｨ ｨｬｨｨ (08ｨｨ09.) ｨｰ ｨ ｨｰｨ, 0906ｨ ｨ 09ｨ, 0800ｨ ｨｰｨ, 09ｨｨ04ｨ ｨｨ ｨ ｨｨ05ｨｨ 06ｨｰ ｨｨ ｨｬ 09 08ｨ 04ｨｪ06ｨｦ ｨｺｨｰ ｨｰｨｨ ﾂ0209ｨｺ06ｨｦ ｨｨ03ｨｪ0609ｨｰｨｹ ｨｰｨ 0800ｨ ｨｰｨ ｨｨ 09ｨｨ04ｨ ｨｨｨｬ0205ｨｨ ｨｨｨｦ ｨｨ ｨｬ02ｨｪｨｰｨｪ04ｨｦ ｨｨ05ｨｹ, ｨｪ06 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ Est-2 ｨｮ ｨｰｨ 0800ｨ ｨｰｨ 06ｨｺｨ 04ｨ 05ｨ 09ｨｹ ｨ ｨ 0302ｨｪｨ ｨｺｨｰ ｨｰｨｨ ﾂ0209ｨｺｨｨ ｨｨ05ｨｨ ｨｨ ｨｬ 0509ｨｰ0208ｨ 04 ｨｮ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ ｨｰ ｨｺ09ｨ ｨｨ01ｨｪ06ｨｦ ｨｬ0700ｨｺ06ｨｦ ｨｪｨｨ0204 Triticum aestivum L. ｨｨ 0802ｨｺ06ｨｬｨ ｨｨｨｪｨ ｨｪｨｰｨｪ0401 ｨｨｨｪｨ ｨｪ ｨｨｨｪｨｨｨｦ ｨｺｨ 08ｨｰｨｨ08ｨｮ06ｨｴ02ｨｦ ｨｮ050702ｨｨｨｨ ITMI. 0106ｨｬ0208ｨ ｨｬ 05ｨｨｨｪｨｨｨｦ ITMI ｨｰ0902ｨｰ09ｨｰ09ｨｮ06ｨｰ 02ｨｨ ｨｪｨ ｨｺｨ ｨｬｨｨ; 02ｨｨ ｨ 09ｨ ｪ ｨｬｨ 08ｨｺ ｨｬ060502ｨｺｨｮ050708ｨｪ06ｨｦ ｨｬｨ , ｨｺ02ｨ ; ｨｹ ｨｺ ｨｪｨｮｨｬ ｨ ｨｪ04 ｨｨ ｨｬ ｨｰ0208ｨ ｨ ｨ ｨｪｨ 08ｨｮ0302ｨｪ06 7, ｪ 8, ｨｮ 05ｨｨｨｪｨｨｨｦ ITMI ｪ 06ｨｰ ｨｨ ｨｬ 0509ｨｰ0208ｨ ｨｬ02ｨｪｨｰｨｪ ｨｨ05ｨｨ 05ｨｨｨｪｨｨｨｦ ITMI 06ｨｰ05ｨｨ ﾂｨ 05ｨｨ09ｨｹ 0108ｨｮ00 06ｨｰ 0108ｨｮ00ｨ ｨｹｨｺ ｨｨ05ｨｨ 05ｨｨｨｪｨｨｨｦ ITMI10 ｨｨ ITMI29 06ｨｺｨ 04ｨ 05ｨｨ09ｨｹ ｨｨ ｨｺｨ ｨｺ 0706 ｨｺｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ, ｨｰｨ ｨｺ ｨｨ 0706 ｨｺ0605ｨｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ ｨｰｨ 09ｨｮ, 0601ｨｪｨ ｨｺ06 06ｨｪｨｨ 06ｨｰ05ｨｨ ﾂｨ 05ｨｨ09ｨｹ 06ｨｰ ｨｨ0502ｨｦ 05ｨｨｨｪｨｨｨｦ ｨｨ , ｨ ｨｰｨ ｨｺ ｨｰ ｨｨ0502ｨｦ ｨｮ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ ｨｰ0609, ﾂｨｰ ｨｰ ｨｨ ｨｹ040609ｨ ｨｰｨｹ ｨｨ ｨ ｨｪｨ 05ｨｨ04ｨ 0708ｨｨ 09ｨｺ0802ｨｴｨｨ09ｨ ｨｪｨｨ ｨ 04ｨｬ ｨｨ04 ｨｨ ｨ ｨｪｨｪ ｨｰ0609 ｨｨ05ｨｨ 09 05ｨｨｨｪｨｨ07ｨｬｨｨ ｨｨ05ｨｨ ｨ 08ｨ ｨ ｨ 08ｨ ｨｺｨｰ0208ｨｪ06 ｨｪｨ 05ｨｨ ﾂｨｨ02 ｨｨ ｨｬ 0509ｨｰ0208ｨ 04 Est-8, Est-9 ｨｨ Est ｨｰ ｨｪｨｪ0609ｨｰｨｹ 06ｨｰｨｬ02 ﾂｨ 05ｨｨ ｨｰ0605ｨｹｨｺ ｨｺｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ ｨｨ ｨｺ0605ｨｨ- ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ ｨｰｨ 09ｨｮ 0509ｨｰ0208ｨ ｨ 0605ｨｹ0302ｨｦ ｨｬ060502ｨｺｨｮ050708ｨｪ06ｨｦ ｨｬｨ ｨｦ ｪ Est-1, Est- 330

5 132, Est-3, Est-4, Est-5, Est-6 ｨｨ Est ｨｰ0208ｨ 0404 Est-2 ｨｨ Est ｨｨ09ｨｮｨｰ09ｨｰ090609ｨ 05ｨｨ ｨｮ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ ｨ 08ｨ , 0601ｨｪｨ ｨｺ06 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ Est-2 06ｨｺｨ 04ｨ 05ｨ 09ｨｹ ｨｮ ｨｰ ｨ ｨｰｨ, 0906ｨ ｨ 09ｨ, 0800ｨ ｨｰｨ ｨｨ 05ｨｨｨｪｨｨｨｦ ITMI7, ITMI83, ITMI115, ｨ ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ Est-4 ｪ ｨｮ ｨｰ ｨ ｨｰｨ, ｨ ｨｨ 05ｨｨｨｪｨｨｨｦ ITMI10, ITMI29, ITMI32, ITMI44, ITMI47, ITMI88, ITMI ｨｬｨ Est-6 ｨ 0405ｨ 06ｨ ｨｪｨ 08ｨｮ0302ｨｪｨ ｨｮ ｨｪ02ｨｪｨｪ ｨｰ0609 ｨｨ ｨｨｨｪｨｨｨｦ ITMI, 04ｨ ｨｨ09ｨｺ0506 ﾂ02ｨｪｨｨ02ｨｬ 05ｨｨｨｪｨｨｨｦ ｨｨ ｨ ｨｺ09ｨｨｨｬｨ 05ｨｹｨｪｨｮ06 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ Est ｨｨ05ｨｨ ｨｮ ｨｰｨ ｨ ｨｨ 05ｨｨｨｪｨｨｨｨ ITMI89, ｨｮ 0609ｨｰｨ 05ｨｹｨｪ ｨｰ0609 ｨｨ 05ｨｨｨｪｨｨｨｦ ITMI 06ｨｪｨ 09ｨ 08ｨｹｨｨ080609ｨ 05ｨ 06ｨｰ ｨｪｨｨ04ｨｺ06ｨｦ ｨｪ02ｨｦ ｨｬｨ Est ｨｨ09ｨｮｨｰ09ｨｰ090609ｨ 05ｨ ｨｮ ｨ 08ｨ , 04ｨ ｨｨ09ｨｺ0506 ﾂ02ｨｪｨｨ02ｨｬ ｨｰｨ ｨ ｨｨ 05ｨｨｨｪｨｨｨｦ ITMI47, ITMI88 ｨｨ ITMI ｨｬｨ Est-7 ｨ 0405ｨ 06ｨ ｨｪｨ 08ｨｮ0302ｨｪｨ ｨｮ ｨｰ ｨ ｨｰｨ, 0906ｨ ｨ 09ｨ ｨｨ ｨ, ｨ ｨｰｨ ｨｺ0302 ｨｮ 05ｨｨｨｪｨｨｨｦ ITMI10, ITMI29, ITMI44, ITMI47, ITMI57, ITMI83 ｨｨ ITMI89. 01ｨ 05ｨｨ ﾂｨｨ02 ｨｨ ｨｬ04 Est-3 06ｨｰｨｬ02 ﾂｨ 05ｨｨ ｨｮ ｨ 08ｨ , ｨｺ0806ｨｬ ｨｰｨ 0906ｨ ｨ 09ｨ, ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ Est-3 ｨ 0405ｨ ｨｪｨ ｨｨｨ 0605ｨｹ0302ｨｦ ｨｮ ｨｰｨ ｨ. 0609ｨｰ0208ｨ 04ｨ Est-5 06ｨｰ09ｨｮｨｰ09ｨｰ090609ｨ 05ｨ ｨｰ0605ｨｹｨｺ06 ｨｮ 05ｨｨｨｪｨｨｨｦ , ITMI29, ITMI83 ｨｨ ITMI115, 0202 ｨｬｨ ｨｺ09ｨｨｨｬｨ 05ｨｹｨｪｨｮ06 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ 06ｨｰｨｬ02 ﾂｨ 05ｨｨ ｨｮ ｨｰ ｨ ｨｰｨ, ｨ ｨｨ 05ｨｨｨｪｨｨｨｨ ITMI89. 06ｨｪｨｰ ｨｪ06, ﾂｨｰ ｨｰ ｨ, 0201ｨｨｨｪ09ｨｰ0902ｨｪｨｪ04ｨｦ 0604ｨｨｨｬ04ｨｦ ｨｨ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ ｨ 08ｨ , 06ｨ 05ｨ 01ｨ ｨｰｨｹ06 ｨｨ ｨｰｨｨ ｨｨ ｨｬ 0509ｨｰ0208ｨ ｨｨ 05ｨｰ06ｨｬ ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ ｨｨ ｨｬ ｨｪ02ｨｦ ｨｬ060502ｨｺｨｮ050708ｨｪ06ｨｦ ｨｬｨ ｨｦ ｨｮ ｨｪ ｨｺｨ 04ｨ 05ｨ 09ｨｹ , ﾂ02ｨｬ ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ ｨｨ ｨｬ 09 ｨｪｨｨ04ｨｺ06ｨｦ ｨｨ ｨｺ06ｨｦ ｨｬ060502ｨｺｨｮ050708ｨｪ06ｨｦ ｨｬｨ ｨｦ ｨｰｨｹ ｨｰ ｨ ｨｬ0603ｨｪ06 ｨｨ ｨｹ040609ｨ ｨｰｨｹ 09 ｨｺｨ ﾂ0209ｨｰ ｨｨｨｰ0205ｨｹ09ｨｺ06ｨｦ ｨｬ ｨｨ ｨｪｨｨｨｨ 09ｨｺ0802ｨｴｨｨ09ｨ ｨｪｨｨ ｨ 04ｨｬ ｨｨ ｨｪ02ｨｪｨｪ ｨｨ ｨｬ02ｨｪｨｰｨｪ06ｨｬｨｮ ｨｺｨｰ08ｨｮ 0509ｨｰ0208ｨ 04 06ｨ 08ｨ ｨｨｨｪｨｨｨｨ ｨｨ ｨｰｨ ｨｺ0302 ｨｬ0600ｨｮｨｰ 0905ｨｮ03ｨｨｨｰｨｹ ｨｨｨｰ0205ｨｹ09ｨｺｨｨｨｬｨｨ ｨｬｨ ｨｬｨｨ 09 09ｨｺ0802ｨｴｨｨ09ｨ ｨｪｨｨ ｨｮ ｨｮ0006ｨｬ ｨｨ05ｨｨ ｨｰｨ 05ｨｹｨｪ04ｨｬｨｨ ｨｨ ｨ ｨｪｨｪ04ｨｬｨｨ 05ｨｨｨｪｨｨ07ｨｬｨｨ ｨｨ ｨｰｨ ｨｬｨｨ ｨｰｨ 0505ｨ ｨｰｨ ｨｨ 0906ｨ ｨ 09ｨ 06ｨ 05ｨ 01ｨ 05ｨｨ ｨｮｨｪｨｨｨｺｨ 05ｨｹｨｪ04ｨｬ ｨｺｨｰ ｨｰｨｨ ﾂ0209ｨｺｨｨｨｬ ｨｺｨｰ0806ｨｬ ｨｨ ｨｬ 0509ｨｰ0208ｨ ｨｰ 0505ｨ ｨｰｨ 06ｨｰ05ｨｨ ﾂｨ 02ｨｰ ｨｰ ｨｰｨ 0906ｨ ｨ 09ｨ ｨｪｨ 05ｨｨ- ﾂｨｨ02ｨｬ ｨｨ ｨｬ04 Est-3 ｨｨ ｨ ｨｺ06ｨｦ ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ06 Est ｨｰｨ 0800ｨ ｨｰｨ ｨｨ 09ｨｨ04ｨ 0106ｨｰｨｹ ｨｨ 01ｨ 08ｨ ｨｺｨｰ0208ｨｨ040609ｨ 05ｨｨ09ｨｹ ｨｨｨｬｨｨ ｨｺｨｰ08ｨ ｨｬｨｨ, ｨｪ06 ｨｮ ｨｰｨ 0800ｨ ｨｰｨ ｨ 0405ｨ 06ｨｰｨｬ02 ﾂ02ｨｪｨ ｨ ｨｺｨ 07 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ Est-2. 09ｨｨｨｪｨｨｨｨ ITMI 06ｨ 05ｨ 01ｨ 05ｨｨ ｨｨｨｬｨｨ ｨｨ0507ｨｬｨｨ ｨｨ ｨｬ 0509ｨｰ0208ｨ 04, 0601ｨｪｨ ｨｺ06 06ｨｰ05ｨｨ ﾂｨ 05ｨｨ09ｨｹ 0108ｨｮ00 06ｨｰ 0108ｨｮ00ｨ ｨｨ 06ｨｰ 05ｨｨｨｪｨｨｨｦ ｨｨ , ｨ ｨｰｨ ｨｺ ｨｰ ｨｰ ｨｨｨｪｨｨｨｨ ITMI7, ITMI83, ITMI89 ｨｨ ITMI115 ｨｨｨｬ0205ｨｨ 0709ｨｪ ｨ 0302ｨｪｨｪ ｨｰ05ｨｨ ﾂｨｨｨｰ0205ｨｹｨｪ0402 ｨｬ ｨｨ ﾂ0209ｨｺｨｨ02 01ｨ 08ｨ ｨｺｨｰ0208ｨｨ09ｨｰｨｨｨｺｨｨ (30), ﾂｨｰ ｨｰ ｨｨ ｨｹ040609ｨ ｨｰｨｹ ｨｨ01 09 ｨｺｨ ﾂ0209ｨｰ0902 ｨｨ ｨｪ ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ 0708ｨｨ ｨｪｨｨｨｨ 0002ｨｪ02ｨｰｨｨｨｺ ｨｺ02ｨｨ06ｨｪｨｪ ｨｺ0802ｨｴｨｨ09ｨ ｨｪｨｨｨｦ. 08ｨ ｨｦ ｨｨ ｨｰ0609 ｨｨ 05ｨｨｨｪｨｨｨｦ 06ｨ 05ｨ 01ｨ 05 06ｨｰ05ｨｨ ﾂｨｪ04ｨｬ 06ｨｰ 0108ｨｮ00ｨｨ01 06ｨ 08ｨ ｨｪ06ｨｰｨｨ0706ｨｬ ｨｪｨｨ05ｨｨ ｨｨ ｨｰ ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ ｨｪｨｪ04ｨｬ 0506ｨｺｨｮ09ｨ ｨｬ, ｨｺ0601ｨｨ08ｨｮ06ｨｴｨｨｨｬ ｨｨ ｨｬ ｨｰ0208ｨ ｨｪ ｨｰ ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ (H) ｨｰｨ 09ｨｨ05ｨ 0,924; 01ｨｨ ｨｨ ｨｰ ｨｨ0006ｨｰｨｪ0609ｨｰｨｨ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ ｨｪｨ ｨ 0608ｨ 06ｨ 08ｨ Var(H) = 0, ｨｺ0605ｨｹｨｺｨｮ 08ｨ 04ｨｬ ｨ 0608ｨｺｨｨ ｨ ｨｰｨｪ0609ｨｨｨｰ0205ｨｹｨｪ06 ｨｬｨ 05 ｨｨ ｨｨｨｬ060800ｨｪ04ｨｬｨｨ 06ｨｺｨ 04ｨ 05ｨｨ09ｨｹ ｨｰ0605ｨｹｨｺ06 5 ｨｨ ｨｬ 0509ｨｰ0208ｨ 04, ｨ 09 ﾂ02ｨｰｨ 0002ｨｰ ｨｨ0006ｨｰｨｪ0609ｨｰｨｨ ｨｬ04 ｨｨ ｨｹ040609ｨ 05ｨｨ , ｨｪｨｪ04ｨｦ 00. Nei (25) ｨｨ ｨｦ08 (26), ｨｺ06ｨｰ060804ｨｦ ｨｨ05 ｨ ｨｰ06 ﾂｨｪ ｨｪｨｨｨｰｨｹ ｨｪ ｨｰ ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ ｨｨ ｨｨ ｨｨ ｨｪｨｪｨ ｨｰ ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ 08ｨ 0909ｨｬｨ ｨｰ08ｨｨ09ｨ 05ｨ 09ｨｹ ｨｺｨ ｨｺ ｨｪ ｨｨ ｨｺｨｮ ｨｮｨｬ07 08ｨ 0405ｨｨ ﾂｨｪ04ｨｬｨｨ ｨ ｨｬｨｨ ｨｪ06ｨｬ 0506ｨｺｨｮ0902 ｨｮ 0601ｨｪ06ｨｦ ｨ ｨｨ ｨｨ ｨｬ060005ｨ ｨ 04ｨｰｨｹ ｨｪｨ ｨｺｨ ｨｺ ｨｪｨ ｨ ｨ 02ｨｬｨ ｨｰ ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ, 01ｨ 08ｨ ｨｺｨｰ0208ｨｨ04ｨｮ06ｨｴｨ 07 ﾂｨ 09ｨｰｨｹ 0002ｨｪ0609, 0706 ｨｺ06ｨｰ060804ｨｬ ｨｮ050702ｨｨ ｨｰ ｨｨ0006ｨｰｨｪｨ (27). 0209ｨｪ0609ｨｪ ｨｰ05ｨｨ ﾂｨｨ02 ｨｨ ｨｹ040609ｨ ｨｪｨｪ0401 ｨｪｨ ｨｬｨｨ 09ｨｰｨ ｨｰｨｨ09ｨｰｨｨ ﾂ0209ｨｺｨｨ ｨ ｨｺ0506 ﾂｨ 02ｨｰ ｨｰ06ｨｬ, ﾂｨｰ06 ｨ 09ｨｰ (25, 26) ｨｨ 0201ｨｨｨｪ0606ｨ 08ｨ 04ｨｪ06 08ｨ ｨｬ0602 ｨｬｨｨｨｪｨｨｨｬｨ 05ｨｹｨｪ ｨｰｨｺ0506ｨｪ02ｨｪｨｨ02 ｨｪ0209ｨｬ02ｨｴ02ｨｪｨｪ06ｨｦ ｨｪｨｺｨｨ 0002ｨｰ ｨｨ0006ｨｰｨｪ0609ｨｰｨｨ (01) ｨｰ0902ｨｰ09ｨｰ09ｨｨｨｨ ｨｰ06 ﾂｨｪ04ｨｬ 04ｨｪｨ ﾂ02ｨｪｨｨ02ｨｬ 01ｨｨ ｨｨｨｨ Var(H) ｨｰ ｨｪｨｨ ｨｮ ﾂｨｨ05ｨｨ ｨｬｨｮ0504, ｨｴｨｨ02 08ｨ 0909 ﾂｨｨｨｰｨ ｨｰｨｹ 0506ｨ 06ｨｦ ｨｬｨｪ ﾂ0502ｨｪ 09 ｨｪｨ ｨ ｨｬ02ｨｪｨｪ0401, 08ｨ ｨｪｨｪ0401 ｨｬｨｮ05ｨｹｨｰｨｨｨｪ06-331

6 13ｨｬｨｨｨ 05ｨｹｨｪ06, ﾂｨｰ06 01ｨ 02ｨｰ ｨｬ0603ｨｪ0609ｨｰｨｹ 08ｨ 0909ｨｬｨ ｨｰ08ｨｨ09ｨ ｨｰｨｹ ｨｬｨｮ ｨｰ ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ ｨｺｨ ｨｺ ｨｬ0208ｨｮ ｨｨｨｪ000608ｨｬｨ 02ｨｨ06ｨｪｨｪ ｨｨｨｬ060800ｨｨ04ｨｬｨ, 0905ｨｮ03ｨ ｨｴ ｨ 03ｨｪ04ｨｬ ｨｺ06ｨｬ0706ｨｪ02ｨｪｨｰ06ｨｬ 0708ｨｨ ｨｰｨ ｨｪｨｨｨｨ 0705ｨ ｨｪ ｨｺ02ｨｨ06ｨｪｨｪ ｨ ｨｬｨｬ ｨｨ 0601ｨｪｨｨｨｬ ｨｨ04 ｨｺ0506 ﾂ ｨｨｨｪ000608ｨｬｨ 02ｨｨ06ｨｪｨｪ06-09ｨｰｨ ｨｰｨｨ09ｨｰｨｨ ﾂ0209ｨｺｨｨ ｨｺｨ 04ｨ ｨｰ020502ｨｦ 0708ｨｨ ｨｨ ｨｪ02ｨｪｨｨｨｨ. 0201ｨｪｨ ｨｺ06 06ｨ ｨｪｨ 08ｨｮ0302ｨｪｨｨ ｨ ｨｺ02ｨｨ06ｨｪｨｪ06 04ｨｪｨ ﾂｨｨｨｬ ｨｦ09ｨｰ09 04ｨ 09ｨｨ09ｨｨｨｰ 06ｨｰ 0002ｨｪ06ｨｰｨｨ07ｨ 06ｨ 08ｨ , ｨ ｨ ｨｰ0205ｨｹｨｪ06, ｨｨｨｪ000608ｨｬｨ 02ｨｨ06ｨｪｨｪ04ｨｦ ｨｨｨｬ060800ｨｨ04ｨｬ 0209ｨｰｨｹ ｨｪ02 ﾂｨｰ06 ｨｨｨｪ0602, ｨｺｨ ｨｺ 06ｨｰ08ｨ 0302ｨｪｨｨ ｨｬ06ｨｦ ｨｪｨ 0002ｨｪ06ｨｰｨｨ07ｨｨ ﾂ0209ｨｺ06ｨｬ ｨｨ(ｨｨ05ｨｨ) ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺ06ｨｬ ｨｮ080609ｨｪ02 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｨ ｨｨ 08ｨ ｨｪｨｨ ｨｪ06ｨｬ ｨｪ02ｨｰｨｨ ﾂ0209ｨｺｨｨ ｨｰ0208ｨｬｨｨｨｪｨ ｨｪｨｰ (0002ｨｪ0609, 0506ｨｺｨｮ ｨｬ060906ｨｬ), ｨｴｨｨ ｨｪｨｨ02 ｨｨ04ｨｮ ﾂｨ 02ｨｬ ｨｨ04ｨｪｨ ｨｺ ｨ ｨｪ0202 ｨ ｨｺｨ 04ｨ ｨｪ06, ﾂｨｰ ｨｪ04, ｨｺ0601ｨｨ08ｨｮ06ｨｴｨｨ02 ｨｨ ｨｬ ｨｰ0208ｨ 04, 08ｨ ｨ 00ｨ 06ｨｰ ｨｬ060906ｨｬ ｨｰ ｨｪ06ｨｬ ｨｺ09ｨ ｨｨ01ｨｪ06ｨｦ ｨｪｨｨ0204 (20-22) ｨｰ ｨｮ02ｨｰ, ﾂｨｰ ｨｨｨｨ ｨｨ ｨｨ05ｨ 01ｨｨ ｨｪ02ｨｨｨ 02ｨｨ07 05ｨｰ06ｨｦ ﾂｨ 09ｨｰｨｨ 0002ｨｪ06ｨｬ ｨｪ ｨｨｨｰｨｹ ｨｺｨ ｨｺ ｨｬｨｨｨｪｨｨｨｬｨｮｨｬ 0109ｨ ｨｬｨｮｨｰｨ 02ｨｨ06ｨｪｨｪ ｨ 04ｨｰｨｨ07, ｨｨ070903ｨｨ01 ｨｪｨ 08ｨ ｨｪｨｨ02 ｨｨ ｨｬ 0509ｨｰ0208ｨ ｨｨ 0006ｨｬ ｨｨ ﾂｨｪ ｨｬ060906ｨｬ. 06ｨｰ06 ｨｬ060302ｨｰ ｨ 04ｨｰｨｹ ｨｰｨ ｨｺ ｨｰｨｪ020902ｨｪ06 ｨｺ 00ｨｨｨ 08ｨｨ01ｨｪ04ｨｬ ｨｬｨ ｨｬ ｨｨ ｨｬ02ｨｪｨｰ0609 (01ｨｨｨｬ020804, ｨｰ08ｨｨｨｬ ｨｨ05ｨｨ ｨｰ02ｨｰ08ｨ ｨｬ ｨｨ ｨｰ.07.), ｨｺ0605ｨｹｨｺｨｮ ｨｮ ﾂ02ｨｪｨｨ02 ｨｰｨ ｨｺｨｨ ｨｬ 06ｨ ｨｮ ｨｪ06 ｨｰ0605ｨｹｨｺ06 ｨｪｨ 05ｨｨ ﾂｨｨ02ｨｬ 08ｨ 0405ｨｨ ﾂｨｪ ｨ 08ｨｨｨ ｨｪｨｰ ｨｨｨｬ ｨｰｨｺｨ ｨｪ0701 ｨｨ ｨ ｨｪｨ 01 08ｨ 09ｨｰ02ｨｪｨｨｨｦ (15, 18, 19). 0606ｨｰ 00ｨ ｨｺｨｰ, ﾂｨｰ ｨｪｨｪ0402 ｨｨ ｨｬ02ｨｪｨｰ04 ｨｺ0601ｨｨ08ｨｮ06ｨｰ ｨｬ ｨｨ ﾂｨｪ04ｨｬｨｨ ｨｬ060906ｨｬｨ ｨｬｨｨ (ｨｨ, ｨｬ0603ｨｪ06, ｨｨ ﾂｨ ｨｬｨｨ), ｨ 09ｨｮ02ｨｰ ｨｨ0706ｨｰ020406ｨｦ ｨ 09ｨｪｨｨｨｰ0205ｨｹｨｪ06 ｨｪ0201ｨ 09ｨｪ02ｨｦ 01ｨｨ ｨｪ02ｨｨｨ 02ｨｨｨｨ 0002ｨｪ06ｨｬ , 00 ｨｨ D ｨｮ ｨｪｨｨ0204 (21) ｨｰ ｨｰ02ｨｰ08ｨ ｨｨ01ｨｪ0609ｨｰｨｨ ｨｺ 0002ｨｺ09ｨ ｨｨ01ｨｪ04ｨｬ ｨｬｨ ｨｬ, ｨｰ ｨ 02ｨｬ04ｨｦ ｨ 0101ｨｨｨｰｨｨ09ｨｪ0609ｨｰｨｹ06 ｨｨ ｨｬ 0509ｨｰ0208ｨ 04, 0708ｨｨ090601ｨｨｨｰ ｨｺ 06ｨ 06ｨ ｨｴｨ 06ｨｴ02ｨｬｨｮ 04ｨ ｨｺ0506 ﾂ02ｨｪｨｨ ｨｬ0603ｨｪ0609ｨｰｨｨ ｨｨ ｨｹ040609ｨ ｨｰｨｹ 09ｨｨ09ｨｰ02ｨｬｨｮ ｨｨ ｨｬ 0509ｨｰ0208ｨ ｨ ｨｪｨｨ04ｨ 02ｨｨｨｨ ｨｨ ｨｮ090205ｨｨ- ﾂ02ｨｪｨｨｨｨ 0705ｨ 09ｨｰｨｨ ﾂｨｪ0609ｨｰｨｨ ｨｨ070506ｨｨ ｨｨ ｨｨ01 ｨ 01ｨ 07ｨｰｨ 02ｨｨｨｨ ｨｺ 08ｨ 0405ｨｨ ﾂｨｪ04ｨｬ ｨｮ ｨｨ07ｨｬ 06ｨｺ08ｨｮ03ｨ 06ｨｴ02ｨｦ ｨｰ ｨ 09ｨｮ02ｨｰ ｨｪｨ ｨ ｨｪｨｨ02ｨｬ, ﾂｨｰ ｨｨ070506ｨｨ ｨ ｨｪ04 ｨ ｨｺｨｰｨｨ09ｨｪ06 ｨｺ060506ｨｪｨｨ04ｨｨ080609ｨ ｨｰｨｹ ｨ 0802ｨ ｨ 09ｨｪ02ｨｪｨｨ06 09 ｨ 05ｨｨ04ｨｺ ｨｰ0902ｨｪｨｪ04ｨｬｨｨ 01ｨｨ070506ｨｨ01ｨｪ04ｨｬｨｨ 09ｨｨ01ｨ ｨｬｨｨ (3, 6). 06ｨ ｨｺｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ, ｨｮ ﾂ02ｨｪｨｪ ｨｮ05ｨｹｨｰｨ ｨｰ ｨｨ05ｨｨ ｨｨｨｰｨｹ ｨｨ04 ｨｨ ｨ ｨｪｨｪ ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ ｨｪｨ ｨｨｨ ｨｺｨｰｨｨ09ｨｪ ｨｺ02ｨｨ06ｨｪｨｪ ｨｨｨｰ0205ｨｹ09ｨｺｨｨ ｨｬ04 ｪ ｨｰｨ 0505ｨ ｨｰｨ, ｨ ｨｨ 05ｨｨｨｪｨｨｨｨ , , ITMI7, ITMI44, ITMI83 ｨｨ ITMI115, ｨｺ06ｨｰ ｨ 0405ｨｨ ﾂｨ 05ｨｨ09ｨｹ ｨｪ02 ｨｰ0605ｨｹｨｺ ｨｪ ｨｨ- ﾂ0209ｨｺｨｨｨｬ ｨｨ 0002ｨｪ06ｨｰｨｨ07ｨｨ ﾂ0209ｨｺｨｨｨｬ 0706ｨｺｨ 04ｨ ｨｰ020507ｨｬ, ｨｪ06 ｨｨ ｨｺｨｰ08ｨ ｨｬ ｨｨ ｨｬ 0509ｨｰ0208ｨ ｨｨ 05ｨｰ06ｨｬ 09ｨｮｨｴ0209ｨｰ09ｨｮ02ｨｰ ｨｬ0603ｨｪ0609ｨｰｨｹ ｨｪｨｨ ｨｨｨｬ060800ｨｨ04ｨｬｨ ｨｪ02 ｨｰ0605ｨｹｨｺ06 ｨｮ ｨｨ ｨｪ ｨｬ, ｨｪ06 ｨｨ ｨｮ ｨ 00ｨ 02ｨｬ ｨｰ06ｨｬｨｺ0609, ｨｮ ﾂ02ｨｪｨｪ ｨｮ05ｨｹｨｰｨ ｨｰ02 09ｨｺ0802ｨｴｨｨ09ｨ ｨｪｨｨｨｦ. 01ｨ 05ｨｨ ﾂｨｨ ｨｰｨｨ ｨｨ ｨｬ ｨ 02ｨｰ 0509ｨｰ0208ｨ ｨｺ09ｨ ｨｨ01ｨｪ06ｨｦ ｨｪｨｨ0204 ｨｮ0106ｨ ｨｪ04ｨｬ ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺｨｨｨｬ ｨｬｨ 08ｨｺ020806ｨｬ ｨｨ ｨｰ ｨｨｨｰｨｹ ｨｺｨ ｨｺ 00ｨｨ04ｨｨ ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺｨｨ02, ｨｰｨ ｨｺ ｨｨ 0002ｨｪ02ｨｰｨｨｨｺ ｨｺ02ｨｨ06ｨｪｨｪ0402 ｨｨ ｨ ｨｪｨｨ ｨｰｨ 05ｨｹｨｪｨ 07 ｨｨｨｪｨｰ ｨｰｨ 02ｨｨ ｨｮ ﾂ02ｨｪｨｪ0401 ｨｨ ｨｬ02ｨｪｨｰｨｪ ｨｨ0502ｨｦ 09ｨｰｨ ｨｪ02ｨｰ ｨｬ0603ｨｪ06ｨｦ ｨ 09ｨｪｨｨｨｰ0205ｨｹｨｪ ｨ ｨｪｨ 05ｨｨ04ｨ ｨｨ ｨｬ02ｨｪｨｰｨｪ ｨｨ0502ｨｦ ｨｮ 01ｨｨ070506ｨｨ01ｨｪ ｨｪ ｨｪ06ｨｬ0609 ｨｨ04ｨｮ ﾂ02ｨｪｨｪ ｨｬ ｨ 03ｨ 02ｨｬ ｨ 05ｨ ｨ 08ｨｪ0609ｨｰｨｹ ﾂ0208ｨｨｨｪ06ｨｦ 04ｨ 0706ｨｬ06ｨｴｨｹ 0708ｨｨ ｨｪｨｨｨｨ 09ｨｰｨ ｨｰｨｨ09ｨｰｨｨ ﾂ0209ｨｺｨｨ01 08ｨ 09 ﾂ02ｨｰ ｨｰ ｨｨ0006ｨｰｨｪ0609ｨｰｨｨ ｨｨ ｨｨ ｨｨｨｨ ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ04ｨｦ ｨｮｨｪｨｨ ｨｨｨｰ02ｨｰ , ｨｮｨ 05ｨｨｨｺｨ ｨ, ｨｨ03ｨｨｨｪ0209, ｨｮ05. 00ｨ ｨｰ020209ｨｨ ﾂ, 60; ｨｺ060909ｨｺｨｨｨｦ ｨｬ ﾂｨｨｨｪ0609ｨｺｨ 03, ｨｨ07, ｨｺ060909ｨｺｨ 07 06ｨ 05., 0201ｨｨｨｪ ｨｺｨｨｨｦ 08-ｨｪ, ｨｨ09ｨ ｨｪ060909ｨｺ0602, ｨｮ05. 08ｨ 05ｨｨｨｪｨｨｨｪｨ, 1; ｨｨ04ｨｨ ﾂ0209ｨｺｨｨｨｦ , ｨｨ07, ｨ ｨｪｨｺｨｰ-0302ｨｰ0208ｨ ｨｮ0800, 0108ｨ 0301ｨ ｨｪ09ｨｺｨｨｨｦ , 14, ｨｨｨｦ09ｨｺｨｨｨｦ ｨｨｨｪ09ｨｰｨｨｨｰｨｮｨｰ 0002ｨｪ02ｨｰｨｨ ﾂ0209ｨｺｨｨ ｨｮ ｨ 09ｨｰ02ｨｪｨｨｨｦ ｨｨｨｬ ｨ 09ｨｨ050609ｨ (000604), ｨｨ07, ｨ ｨｪｨｺｨｰ-0302ｨｰ0208ｨ ｨｮ0800, ｨｮ ｨｹ03ｨ ｨｺｨ 07, 42-44, yu.chesnokov@vir.nw.ru ｨｰｨｮ07ｨｨ05ｨ ｨ ｨｺ02ｨｨ06 25 ｨｬｨ 08ｨｰｨ ｨ 332

7 13Sel ｯskokhozyaistvennaya biologiya [Agricultural Biology], 2016, V. 51, 01 3, pp ISOZYMIC ANALYSIS OF ESTERASES IN MATURE SEEDS OF HEXAPLOID SOFT WHEAT (Triticum aestivum L.) A.S. Rudakova 1, S.V. Rudakov 1, N.V. Davydova 2, G.V. Mirskaya 3, E.V. Zhuravleva 2, Yu.V. Chesnokov 4 1 State University of Moldova, 60, str. Mateevich, Kishinev, 2009 Moldova; 2 00Nemchinovka03 Moscow Research Institute for Agriculture, Federal Agency of Scientific Organizations, 1, ul. Kalinina, RP Novoivanovskoe, Odintsovo Region, Moscow Province, Russia; 3 Agrophysical Research Institute, Federal Agency of Scientific Organizations, 14, Grazhdanskii prosp., St. Petersburg, Russia; 4 Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources, Federal Agency of Scientific Organizations, 42-44, ul. Bol ｯshaya Morskaya, St. Petersburg, Russia, yu.chesnokov@vir.nw.ru Acknowledgements: We are grateful to Dr. N.V. Kocherina for help with the statistical calculations of heterozygosity and its dispersion. Received March 25, 2016 doi: /agrobiology eng Abstract Esterases represent a large group of enzymes that catalyze cleavage of multiple-ester bonds. In general, they are divided into four types: cholinesterases (most frequently, these are identified using ordinary electrophoretic analysis), acetylesterases, arylesterases and carboxyl esterases. Plant carboxyl esterases catalyze conversion of the esters into bioactive acids and alcohols, thereby playing a key role in many biological processes. Lack of epistatic interactions as well as a co-dominant nature of the inheritance of the esterase isozymes makes them meaningful for quick and accessible investigation of the processes of biochemical adaptation to environmental changes. Such a type of markers, which is convenient for solution of practical problems of selection, can be used as a tool that can speed up and simplify the selection of the significant material. The aim of this study was to estimate the isoenzyme profile of esterases isolated from mature seeds, and to ascertain, using such a biochemical marker, the polymorphism among samples of promising breeding material of the hexaploid wheat (Triticum aestivum L.). Ripe seeds from following wheat cultivars were used as samples: Zlata, Lyubava, Agatha, Lisa (spring wheat) and Mera (winter wheat) (originated by Moscow Agricultural Research Institute 00Nemchinovka03, Moscow Province); lines AFI91 and AFI177 (spring wheat) and recombinant inbred lines of the mapping populations ITMI ｪ 7, 10, 29, 32, 44, 47, 57, 83, 88, 89, and 115 (spring wheat) (originated by Agrophysical Research Institute ｪ AFI, St. Petersburg). The seeds were ground in a porcelain mortar and the flour was sieved. The enzymes were extracted from the flour and subjected to vertical native electrophoresis using 4 % concentrating and 8 % separating polyacrylamide gels. Molecular weight markers were Page Ruler Prestained Protein Ladder 00Thermo Scientific03 (Lithuania). After electrophoresis, gels were treated with a reagent for a nonspecific esterase and scanned. Individual electrophoretic profile of each sample was estimated. Heterozygosity and its dispersion were calculated. The esterase complex in the wheat seeds studied was represented by 10 isoforms. From 9 to 10 isoforms of various electrophoretic mobility have been identified in cultivars Zlata, Lyubava, Agatha, Lisa and Mera, 7 isoforms were found in line AFI91, 8 isoforms ｪ in AFI177, and from 7 to 10 isoforms ｪ in ITMI lines. All samples were characterized by the presence of esterases isoforms Est-8, Est-9 and Est-10. Heterogeneity was only found in the qualitative and quantitative composition of esterases with a greater molecular weight ｪ Est-1, Est-2, Est-3, Est-4, Est-5, Est-6, and Est-7. Each variety among eighteen varieties and lines had a genotype different from that in the other samples. The average heterozygosity (H) of samples within 10 loci encoding esterase isoforms was 0.924; the dispersion of heterozygosity for all the samples studied was Var(H) = As a result of the analyses, following most promising breeding parental forms are varieties Zlata and Mera, and lines AFI91, AFI177, ITMI7, ITMI44, ITMI83, and ITMI115. Because of the existence of ten isoforms in hexaploid wheat, the esterases might represent a convenient biochemical marker suitable for examination of samples of the hexaploid wheat at physiological, biochemical, and genetic levels. Keywords: hexaploid soft wheat, mature seeds, isozymic analysis, esterase REFERENCES 1. Bou lte r D., Thur ma nn D.A., Tu r ne r B.L. The use of disc electrophoresis of plant proteins in systematics. Taxon, 1966, 15: (doi: / ). 2. S h a w C.R. Isozymes: classification, frequency, and significance. Int. Rev. Cytol., 1969, 25: (doi: /S (08) ). 3. H a r t G.E. Genetics and evolution of multilocus isozymes in hexaploid wheat. Isozymes Curr. Top. Biol. Med. Res., 1983, 10:

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