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1 , 2013, 01 5, ｨｨｨｺ0806ｨ ｨｨ ｨｨ ﾂ /.8:631.46:574.24: '1 doi: /agrobiology rus ﾁ S * , , , , ｨｨ0608ｨ 04ｨｪ0606ｨ 08ｨ 04ｨｨ ﾂ0902ｨｪｨｪ0401 ｨｬｨｨｨｺ0806ｨ ｨｪ ｨ ｨｴ0209ｨｰ09 09 ｨｪｨ 09ｨｰ0607ｨｴ ｨｬ07 ｨｨ04ｨｮ ﾂｨ 02ｨｰ ｨｰｨ ｨｰ06 ﾂｨｪ06 03ｨｨ0806ｨｺ06. 02ｨ ｨｲ02ｨｺｨｰ06ｨｬ ｨｪｨ 03ｨｨ01 ｨｨ ｨ ｨｪｨｨｨｦ ｨ 0405ｨ 09ｨｰ08ｨｮｨｺｨｰｨｮ08ｨ ｨ ｨｴ0209ｨｰ09ｨ ﾂ ｨｨｨｰ0205ｨｹｨｪ ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ ｨｰｨ ｨｺ060909ｨｺ06ｨｦ ｨｹ09ｨｺ ｨｦ09ｨｰ0902ｨｪｨｪ06ｨｦ ｨ ｨｺｨ 0102ｨｬｨｨｨｨ, 04ｨ ｨｪｨｪ ｨｮ ｨ ｨｪｨ 05ｨｨ04ｨｨ080609ｨ 05ｨｨ ｨｦ09ｨｰ09ｨｨ02 ｨｪｨ 0706 ﾂ0902ｨｪｨｪ04ｨｦ ｨｬｨｨｨｺ0806ｨ ｨｨ06ｨｬ 08ｨ 0405ｨｨ ﾂｨｪ ﾂ02ｨｰｨ ｨｪｨｨｨｦ ｨｰ ｨ ｨｰ0201ｨｪｨｨ ﾂ0209ｨｺｨｨ01 00ｨ ｨｺｨｰ ｪ ｨ 02ｨｬ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0804 (0502ｨｪ ｨｨ 0604ｨｨｨｬｨ ｨｹ), ｨ ｨｰｨ ｨｨ ｨｨ ｨｰｨｺ0609ｨ ｨｪｨｨ ﾂ ｨ ｨｪｨｪ ｨ ｨｨ0608ｨ 04ｨｪ0606ｨ 08ｨ 04ｨｨ06 ｨ 0405ｨｨ ｨｮ ﾂ02ｨｪ ｨｨ 0706ｨｬ06ｨｴｨｨ ｨｬ02ｨｰ0601ｨ ｨｺ ｨｨ ｨｨｨｰ0205ｨｹｨｪ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07. 06ｨ ｨｺ0302 ｨｬ ｨｨｨｬ02ｨｪｨｨ05ｨｨ 0608ｨｨ00ｨｨｨｪｨ 05ｨｹｨｪ04ｨｦ ｨｺ ｨ ｨｪｨ 05ｨｨ04ｨｮ 01ｨ ｨｪｨｪ ｨｺ ｨｨ ｨｨｨｰ0205ｨｹｨｪ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 ｪ ｨｬ02ｨｰ ｨ ｨｨｨｪｨ 08ｨｪ ｨｬ0705ｨｨｨｪ00ｨ ｨｺｨ 04ｨ ｨｪ06, ﾂｨｰ06 09 ｨｺ0605ｨｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬ 06ｨｰｨｪ060302ｨｪｨｨｨｨ 0008ｨｮ0707ｨ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609, ｨｨ04ｨｬ02ｨｪ0706ｨｴｨｨ ｨｨ07ｨｪｨｨ02ｨｬ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ ｨ ｨｺｨｰ , 0708ｨｨｨｬ0208ｨｪ06 08ｨ 09ｨｪｨ 0008ｨｮ ｨｪ02 ｨｨ04ｨｬ02ｨｪ0706ｨｴｨｨ ｨｦ09ｨｰ09ｨｨ02ｨｬ 05ｨｰｨｨ01 00ｨ ｨｺｨｰ ｨｪｨ ｨｺ06 05ｨｰｨｨ 0008ｨｮ ｨｨ05ｨｹｨｪ06 08ｨ 0405ｨｨ ﾂｨ 05ｨｨ09ｨｹ 0706 ｨｺｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ ｨｰｨ 09ｨｮ: 0008ｨｮ0707ｨ, ｨｪｨｪｨ ｨｨ07ｨｪｨｨ06, ｨ 0405ｨ ｨ ｨｪ ｨｪｨ. 06ｨ ｨｺｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ, ｨｬ0603ｨｪ06 ｨｮｨｰ ｨ ｨｰｨｹ, ﾂｨｰ ｨｦ09ｨｰ09ｨｨ ｨ 02ｨｰ0907 ｨｪ02 06 ﾂ02ｨｪｨｹ ｨ 0605ｨｹ03ｨ 07 ﾂｨ 09ｨｰｨｹ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609, ｨ ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｹ 0108ｨｮ00ｨｨ ｨｰｨ 02ｨｰ0907 ｨ ｨｨ05ｨｨ ｨｬ02ｨｪ ｨｰｨ ｨ ｨｨ05ｨｹｨｪ06ｨｦ ｨｪ04 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺｨｨ ｨｮ ｨ ｨｪｨｨ04ｨｬ0609, ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0402 ｨｺ ｨｺｨ ｨｬｨｮ ｨｨ04 ｨｨ04ｨｮ ﾂ02ｨｪｨｪ ｨ ｨｺｨｰ ｨ 00ｨ 02ｨｬ04ｨｦ ｨｪｨ ｨｬｨｨ ｨｨ ｨｹ040609ｨ ｨｪｨｨ02ｨｬ 00ｨ ｨｨｨｪｨ 08ｨｪ ｨｬ0705ｨｨｨｪ00ｨ 03 01ｨ 02ｨｰ ｨｬ0603ｨｪ0609ｨｰｨｹ ｨｰｨｪ0209ｨｰｨｨ ｨｺ06ｨｪｨｺ0802ｨｰｨｪ0402 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 09 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ06ｨｦ 09ｨｰ08ｨｮｨｺｨｰｨｮ0802 ｨｬｨｨｨｺ0806ｨ ｨｪ ｨ ｨｴ0209ｨｰ09ｨ ｨｦ09ｨｰ09ｨｨ02ｨｬ ｨｪｨｪ ｨｺ ｨｨ ﾂ0209ｨｺ ｨ ｨｺｨｰ0608ｨ ﾂ ｨ : ｨｬｨｨｨｺ0806ｨ ｨｨ06ｨｬ 0706 ﾂ0904, ｨｺ ｨｨ ｨｨｨｰ0205ｨｹｨｪ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ ｨｨｨｰ0205ｨｹｨｪ ｨｰ04 ｨｨ ｨｹ04ｨｮ06ｨｰ ｨｰ08ｨ ｨｪｨ 01 ｨｬｨｨ08ｨ ｨｺ06ｨｬ070502ｨｺ09ｨｪ0401 ｨｨ ｨ ｨｪｨｨｨｦ 09 06ｨ 05ｨ 09ｨｰｨｨ ｨ ｨｰ0201ｨｪｨｨｨｺｨｨ ｨｨ 0905ｨｨ07ｨｪｨｨ07 08ｨ 0405ｨｨ ﾂｨｪ ｨ ｨｺｨｰ ｨｪｨ ｨｮ080603ｨ ｨｦｨｪ0609ｨｰｨｹ. 00ｨｪ060006ｨｺ08ｨ ｨｰｨｪｨ ｨｨ ｨｨｨｬ0609ｨｰｨｹ 05ｨｰｨｨ01 05ｨｺ ｨｨｨｬ02ｨｪｨｰ ｨｬ02ｨｪｨｨ 0708ｨｨ090601ｨｨｨｰ ｨｺ ｨｮ09ｨｰｨ ｨｪ ｨｪｨｨ06 ｨｺｨ ﾂ0209ｨｰ0902ｨｪｨｪ06 ｨｪ ｨ ｨｺ06ｨｪ06ｨｬ0208ｨｪ0609ｨｰ02ｨｦ, ｨｺ06ｨｰ ｨｪ ｨｬ0603ｨｪ ｨｨｨｰｨｹ 0708ｨｨ ｨｪｨｨｨｨ ｨｺ08ｨ ｨｰｨｺ ﾂｨｪ ｨｰ ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ04ｨｦ ｨｰ ｨｺ060909ｨｺ06ｨｦ ｨｹ09ｨｺ ｨｦ09ｨｰ0902ｨｪｨｪ06ｨｦ ｨ ｨｺｨ 0102ｨｬｨｨｨｨ ( ｨｨｨｦ09ｨｺｨｨｨｦ ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ04ｨｦ ｨ 0008ｨ 08ｨｪ04ｨｦ ｨｮｨｪｨｨ ｨｨｨｰ02ｨｰｨ ｪ ｨｨｨｬ ｨｨｨｬｨｨ ｨ ), ｨｺ06ｨｰ060804ｨｦ ｨｬ04 ｨｨ ｨｹ040609ｨ 05ｨｨ 09 ｨｪｨ 0302ｨｬ ｨｨ ｨ ｨｪｨｨｨｨ, ｨ ｨ ｨｪ ｨｮ ｨｪｨｺ ｨｬ06ｨｬ02ｨｪｨｰｨ 0609ｨｪ0609ｨ ｨｪｨｨ07 05ｨｰ06ｨｰ ｨｰ 06ｨｰ05ｨｨ ﾂｨ ｨｰ ｨｰ09ｨｮ06ｨｴｨｨ01 ｨｬｨｪ ｨ ｨｺｨｰ0608ｨｪ06ｨｦ ｨｬ06ｨｦ: 09 ｨｺ06ｨｬ070502ｨｺ0902 ｨｨ04ｨｮ- ﾂｨ ｨｹ 0905ｨｨ07ｨｪｨｨ02 ｨｮ0106ｨ 0802ｨｪｨｨｨｦ, ｨｬ06ｨｪ06ｨｺｨｮ05ｨｹｨｰｨｮ08 ｨｨ ｨｪ060202ｨｪｨｪ ｨ ｨｰｨ. 04ｨ ｨｪ0202 ｨｨ ｨ ｨｪｨｨ07 ｨ ｨｨ0608ｨ 04ｨｪ0606ｨ 08ｨ 04ｨｨ07 ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ ｨｨ05ｨｨ09ｨｹ ｨｬ 0706 ﾂ0902ｨｪｨｪ06ｨｦ 09ｨｮ090702ｨｪ04ｨｨｨｨ ｨｪｨ 07ｨｨｨｰｨ ｨｰ0205ｨｹｨｪ ｨｪｨ 09ｨｰ0607ｨｴ ｨｬ07 ｨｪｨ ｨｮｨｺｨ 08ｨ ｨ 00ｨ 02ｨｬ ｨｬ02ｨｰ0601ｨ ｨｬｨｨ, ｨｺ06ｨｰ ｨｰ ｨ ｨｪ ｨ 08ｨ ｨｺｨｰ0208ｨｨ040609ｨ ｨｰｨｹ ｨｰｨ 09 ｨｬｨｨｨｺ0806ｨ ｨｪ ｨ ｨｴ0209ｨｰ09ｨ, ｨｮ ﾂｨｨｨｰ0409ｨ 07 ｨｪ02 ｨｰ0605ｨｹｨｺ06 ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ08ｨｮ02ｨｬ0402, ｨｪ06 ｨｨ ｨｪ02ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ08ｨｮ02ｨｬ0402 ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ04 (1-4). 0201ｨｪｨ ｨｺ06 02ｨｴ02 ｨｪ0201ｨ 09ｨｪ ｨｪｨｨ02 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ ｨｰｨ 09ｨ ｨｬｨｨｨｺ0806ｨ ｨｪ ｨ ｨｴ0209ｨｰ09ｨ 09 ｨｨ ｨｹ040609ｨ ｨｪｨｨ02ｨｬ 05ｨｰｨｨ ｨｨ02ｨｬ0609 ｨ ｨｰｨ ｨｰ06 ﾂｨｪ06 ｨｰ08ｨｮ010602ｨｬｨｺｨｨｨｬ ｨｨ ｨｨｨｬ ｨ 0609ｨ 05ｨｨ09ｨｹ ｨｮ0804 ｨｺ0506ｨｪｨｨ080609ｨ ｨｪｨｨ07 ｨｨ ｨｮ06ｨｴ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 06ｨｰ010205ｨｹｨｪ0401 ｨｪｨｮｨｺ050206ｨｰｨｨ01ｨｪ ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ, ﾂｨｰ06 09ｨｮｨｴ0209ｨｰ0902ｨｪｨｪ ｨ ｨｪｨｨ ﾂｨｨ09ｨ 0506 ｨｺｨ ｨｺ ﾂｨｨ ｨｬ ｨｨ010609, ｨｰｨ ｨｺ ｨｨ 01ｨｨｨ 07ｨ 0406ｨｪ ｨｨ ｨｮ02ｨｬ0401 ｨｬ0209ｨｰ 06ｨ ｨｨｨｰｨ ｨｪｨｨ07 (5, 6). 0308ｨｨｨｬ02ｨｪ02ｨｪｨｨ02 07ｨｨ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 09ｨｮｨｴ0209ｨｰ0902ｨｪｨｪ06 08ｨ 0903ｨｨ08ｨｨ ｨｬ0603- * 04ｨ ｨ 06ｨｰｨ ｨｪ02ｨｪｨ 0708ｨｨ ｨｺ02 00ｨｨｨｪｨｨ09ｨｰ020809ｨｰ09ｨ 06ｨ 08ｨ ｨ ｨｪｨｨ07 ｨｨ ｨｪｨ ｨｮｨｺｨｨ 0408, ｨｨ 0008ｨ ｨｪｨｰｨ ｨ. 85

2 13ｨｪ0609ｨｰｨｨ ｨｬｨ 0903ｨｰｨ ｨ ｨｪ ｨｨ04ｨｮ ﾂ02ｨｪｨｨ07 ｨｬｨｪ060006ｨｺ06ｨｬ0706ｨｪ02ｨｪｨｰｨｪ ｨｺ0609ｨｨ09ｨｰ02ｨｬ, 09ｨｺ0506 ﾂｨ ﾂ0902ｨｪｨｪ0402 ｨｬｨｨｨｺ0806ｨ ｨｪ ｨｰ ｨｬ ｨｮ05ｨｹｨｰｨ ｨｰ04 07ｨｨ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 ｨｨｨｬ0206ｨｰ 06 ﾂ02ｨｪｨｹ ｨｪｨｮ06 09ｨｰ08ｨｮｨｺｨｰｨｮ08ｨｮ, 09 ｨｰ08ｨｮ0106ｨｬ ｨ 06ｨｰ0907 ｨ ｨｪｨ 05ｨｨ04ｨｮ, ｨｨ ｨｰ06ｨｬｨｮ ｨｰ060006, ﾂｨｰ06ｨ 04 08ｨ 0406ｨ 08ｨ ｨｰｨｹ ｨｨ ｨｬ ｨｬｨｪ ｨ 08ｨ 04ｨｨｨｨ, ｨｪ0206ｨ ｨｨｨｬ06 ｨｪｨ ｨｨｨｰｨｹ ｨｪ ｨｺ 09ｨｨ09ｨｰ02ｨｬｨ ｨｰｨｨ04ｨ 02ｨｨｨｨ 01ｨ ｨｪｨｪ ｨｰｨ ｨｬ06ｨｦ 08ｨ ｨ 06ｨｰ02 ｨｬ ｨ 00ｨ 02ｨｬ 0608ｨｨ00ｨｨｨｪｨ 05ｨｹｨｪ04ｨｦ ｨｺ ｨ ｨｪｨ 05ｨｨ04ｨｮ 01ｨ ｨｪｨｪ0401 (ｨｬ02ｨｰ ｨ ｨｨｨｪｨ 08ｨｪ ｨｬ0705ｨｨｨｪ00ｨ 03), ｨｪｨ 0708ｨ ｨｪｨｪ04ｨｦ ｨｪｨ ｨｪｨｨ ｨ 02ｨｪｨｪ0609ｨｰ02ｨｦ ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ06ｨｦ 09ｨｰ08ｨｮｨｺｨｰｨｮ ﾂ0902ｨｪｨｪ ｨｬｨｨｨｺ0806ｨ ｨｨ06ｨｬｨ, ｨ ｨｪｨｪ ｨｦ09ｨｰ09ｨｨ02ｨｬ ｨｪｨｪ ｨｺ ｨｨ ﾂ0209ｨｺ ｨ ｨｺｨｰ0608ｨ. 05ｨｬ ｨｬ02ｨｰ0601ｨ 04ｨ ｨｺ0506 ﾂｨ 02ｨｰ ｨ ｨｪｨｨｨｨ (02ｨｴ02 ｨｪｨ 05ｨｰｨ ｨｰｨ 0608ｨ ｨ ) ｨｰｨ ｨｺ ｨｪｨ ｨ 0608ｨ 06ｨ 08ｨ , ﾂｨｰ06ｨ 04 ｨｨ01 ｨｬ0603ｨｪ06 ｨ ｨ ｨｨｨｰｨｹ ｨｪｨ 0008ｨｮ070704, ｨｺ06ｨｰ ｨ 0405ｨｨ ﾂｨ 05ｨｨ09ｨｹ ｨ 04 ｨｰ0605ｨｹｨｺ ｨｪ06ｨｬｨｮ ｨｨ ｨｦ09ｨｰ09ｨｮ06ｨｴｨｨ01 05ｨｺ ｨｨ ﾂ0209ｨｺｨｨ01 00ｨ ｨｺｨｰ , ｨ ｨｬ 0108ｨｮ00ｨｨｨｬ ｨ 0405ｨｨ ｨ ｨｪ04. 06ｨｬ02ｨｪｨｪ06 ｨｰｨ ｨｺｨ ｨｬｨ 06ｨｰｨ 0608ｨ ｨ ｨｨｨｰ ｨｰｨｹ 0008ｨｮ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609, ｨｬｨ 08ｨｺｨｨ08ｨｮ06ｨｴｨｨ02 ｨｪｨ ｨｨｨ ｨｮｨｴ0209ｨｰ0902ｨｪｨｪ ｨ 0405ｨｨ ﾂｨｨ07 09 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ06ｨｦ 09ｨｰ08ｨｮｨｺｨｰｨｮ0802 ｨｬｨｨｨｺ0806ｨ ｨｨ06ｨｰ04, ｨｺ06ｨｰ ｨ ｨｪ ｨｨ07ｨｪｨｨ02ｨｬ ｨｨ ｨｮ02ｨｬ ｨ ｨｺｨｰ0608ｨ. 0308ｨｨ ｨｰｨ ｨｪｨｨｨｨ 06ｨ ｨｲ0201ｨｨｨｪ02ｨｪｨｪ ｨ 08ｨ ｨｪｨｪ06ｨｬｨｮ 00ｨ ｨｺｨｰ0608ｨｮ ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺ ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ ｨｬ0603ｨｪ ｨｨｨｰｨｹ ｨｰ02 ｨｰｨ ｨｺ0906ｨｪ04, ｨｺ06ｨｰ ｨｬ06ｨｪ09ｨｰ08ｨｨ08ｨｮ06ｨｰ ｨｪｨ ｨｨｨ ｨｪｨ ﾂｨｨｨｬ ｨ 0405ｨｨ ﾂｨｨ ｨｹ06 08ｨ ｨ 06ｨｰ04 ｨ ｨｨ ｨ ｨｪｨｨ02 ｨｬｨｨｨｺ0806ｨ ｨｪ ｨ ｨｴ0209ｨｰ09ｨ ﾂ09ｨ ｨｨｨｰ0205ｨｹｨｪ ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ ｨｰｨ ｨｬ02ｨｰ060106ｨｬ ｨｺ ｨｨ ｨｨｨｰ0205ｨｹｨｪ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ ｨｨｨｬ02ｨｪ02ｨｪｨｨ02ｨｬ ｨｪｨｪ ｨｪｨ ｨｬｨｨ 00ｨ ｨｨｨｪｨ 08ｨｪ ｨｬ0705ｨｨｨｪ00ｨ ｨｰ0601ｨｨｨｺｨ. 0502ｨｬ0205ｨｹｨｪ04ｨｦ ｨｮ ﾂｨ 09ｨｰ06ｨｺ ｨｬｨｪ ｨｰｨｪ ｨｰｨ ｪ ｨｨｨｬ ｨｨｨｬｨｨ ｨ ｨｴｨ 01ｨｹ06 06ｨｺ ,5 00ｨ 09 ｨｮｨｺ0506ｨｪ06ｨｬ 1 ｨｪｨ ｨ 07ｨ 01 08ｨ ｨｪ ｨｪｨ 0603ｨｪ06ｨｦ 06ｨｺ08ｨ ｨｨｨｪ ｨｨｨｪ09ｨｺ06-02ｨｬｨｨｨｰ ｨｺ06ｨｦ ｨｪｨｪ0609ｨｰｨｨ, ｨｰｨ ｨｪｨｪ06ｨｦ ｨｬ060802ｨｪｨｪ06ｨｦ 08ｨ 09ｨｪｨｨｨｪ06ｨｦ ｨｪｨｨ02 ｨｪｨ 01 ｨｮ080609ｨｪ02ｨｬ ｨｬ ｨｬ ｨｪ02ｨｬｨｪ ｨｰｨｪ0202 ｨｺ0605ｨｨ ﾂ0209ｨｰ ｨ 01ｨｺ ｨｺ ｨｬｨｬ , ｨｨ04 ｨｪｨｨ ｨｨｨｬ0208ｨｪ ｨｨｨｪｨ ｪ 04ｨ ｨｬｨ ｨｦ ｪｨ 0900ｨｮ09ｨｰ, ｨｪ ｨ 07 ｨｰ02ｨｬ070208ｨ ｨｰｨｮ08ｨ 4,1 縺 ﾂ09ｨ ｨｪ ｨｨ09ｨｰｨ 07, 09ｨｮ ﾂｨ ｨｪｨ ｨｰｨ ﾂ0902ｨｪｨｪ ｨ 08ｨ ｨｨ05ｨｨ 09 ｨｨ06ｨｪ ｨ ｨｮｨ ｨｨｨｪ04 07ｨ 0106ｨｰｨｪ ｨｨ0406ｨｪｨｰｨ 081 (20 09ｨｬ) ｨｹｨｬｨｨ 09ｨ 08ｨｨｨ ｨｪｨｰｨ ｨｰｨ (09 04ｨ 09ｨｨ09ｨｨｨｬ0609ｨｰｨｨ 06ｨｰ 0906 ﾂ02ｨｰｨ ｨｪｨｨ ｨｦ09ｨｰ09ｨｮ06ｨｴｨｨ01 ｨ ｨｰ0201ｨｪｨｨ- ﾂ0209ｨｺｨｨ01 00ｨ ｨｺｨｰ ) ｨｪｨｨ ｨ ｨｨ08ｨｺｨｮ 06ｨ ｨｲ02ｨｬ06ｨｬ 2 ｨｬ05 06ｨｰｨ ｨｨ08ｨ 05ｨｨ ｨｪｨ ｨｺｨｮ 0706 ﾂ0904 (0,2 00). 0206ｨ ｨ ｨｨ 08ｨ 09ｨｪ ｨ ｨｲ02ｨｬｨｮ ｨｺ0605ｨｨ ﾂ0209ｨｰ ｨ 08ｨｨｨｺ ｨｨｨ ｨｬ02ｨｰ0806ｨｬ 0,1 ｨｬｨｬ (00Innomed03, 0002ｨｪ0008ｨｨ07), 350 ｨｬｨｺ05 08ｨ 09ｨｰ090608ｨ 08 (ｨｪｨ ｨｰ08ｨｨｨｦ ｨ ｨｰｨｪ04ｨｦ ｨ ｨｮ ｪ 20 ｨｬM, ｨｨ0406ｨｰｨｨ0602ｨｨｨ ｨｪｨ ｨｰ 00ｨｮｨ ｨｪｨｨ01ｨｨｨｪｨ ｪ 240 ｨｬM; ,0), 350 ｨｬｨｺ05 08ｨ 09ｨｰ090608ｨ 09 (0608ｨｨ09-01Cl ｪ 500 ｨｬM, SDS ｪ 1 % 0706 ｨｬｨ ｨｺ 06ｨ ｨｲ02ｨｬｨｮ; ph 7,0) ｨｨ 400 ｨｬｨｺ05 09ｨｬ0209ｨｨ 0002ｨｪ ｨｬ ｨ ｨｨ08ｨｺｨｮ 0706ｨｬ02ｨｴｨ 05ｨｨ ｨｰ080701ｨｨ09ｨ ｨｰ0205ｨｹ ｨｨ 08ｨ 0408ｨｮ03ｨ 05ｨｨ 06ｨ 08ｨ ｨｰ02 ﾂ02ｨｪｨｨ ｨｬｨｨｨｪ. 05ｨ ｨｰ02ｨｬ 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ 0708ｨｨ g 09 ｨｰ02 ﾂ02ｨｪｨｨ02 5 ｨｬｨｨｨｪ ｨｪｨｮ06 00ｨ 04ｨｮ 06ｨｰｨ ｨｨ08ｨ 05ｨｨ, 0106ｨ ｨ ｨｨ 400 ｨｬｨｺ ｨｬｨ, ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ ｨｰ080701ｨｨ09ｨ 05ｨｨ 1-3 ｨｬｨｨｨｪ, 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ ｨｰｨ ｨｺ 0302, ｨｺｨ ｨｺ ｨｪｨ ｨｮｨｴ02ｨｦ 09ｨｰｨ 01ｨｨｨｨ, 06ｨｰｨ ｨｨ08ｨ 05ｨｨ ｨｪｨｮ06 00ｨ 04ｨｮ. 08 ｨｪ0206 ﾂｨｨｨｴ02ｨｪｨｪ06ｨｬｨｮ 05ｨｺ09ｨｰ08ｨ ｨｺｨｰｨｮ ｨ ｨ ｨｨ 0601ｨｨｨｪ 06ｨ ｨｲ02ｨｬ ｨｨ ｨｨ ｨｨ08ｨｰｨ, ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ ｨｰ080701ｨｨ09ｨ 05ｨｨ, 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ, ｨｬ0409ｨ 05ｨｨ 70 % 05ｨｰｨ ｨｪ060506ｨｬ, ｨｺｨ ｨｮ03ｨｨ09ｨ 05ｨｨ ｨｪｨ ｨｮ0102 ｨｨ 08ｨ 09ｨｰ ｨｨ 0609ｨ 0106ｨｺ 0708ｨｨ 65 縺05 09 ｨｰ02 ﾂ02ｨｪｨｨ02 15 ｨｬｨｨｨｪ ｨｬｨｺ ﾂｨｨ09ｨｰｨｺｨｨ ｨｰ 0708ｨｨｨｬ020902ｨｦ ｨｨ05ｨｨ ｨｺｨｰ % ｨ 00ｨ (0, ｨ ｨｮ000208) ｨ ｨｪｨｪ04ｨｦ ｨ 0506ｨｺ, ｨ ｨｴｨｨｨｦ , 0706ｨｬ02ｨｴｨ 05ｨｨ ｨ ｨｨ08ｨｺｨｮ ｨｰｨｨ07ｨ ｨｪ (1,5 ｨｬ05), 0106ｨ ｨ ｨｨ 2 06ｨ ｨｲ02ｨｬｨ 08ｨ 09ｨｰ090608ｨ 00 (ｨｨ0406ｨｰｨｨ0602ｨｨｨ ｨｪｨ ｨｰ 00ｨｮｨ ｨｪｨｨ01ｨｨｨｪｨ ｪ 3 00, 0608ｨｨ09-HCl ｪ 20 ｨｬM, 0608ｨｨｨｰ06ｨｪ X-100 ｪ 20 ｨｬ00/ｨｬ05; ,0) ｨｨ ｨｨｨｪｨｺｨｮｨ ｨｨ080609ｨ 05ｨｨ 0708ｨｨ 65 縺 ｨｪ ｨ 09ｨｰ ｨｪｨｨ07 ｨ 0506ｨｺｨ ｨ 09ｨｰ090608ｨｮ 0708ｨｨ05ｨｨ09ｨ 05ｨｨ 20 ｨｬｨｺ05 08ｨ 09ｨｰ090608ｨ 01 (08ｨ 09ｨｰ ｨ ｨ ｨｪｨｨ02ｨｬ ｨｰ06ｨｪｨｺ0601ｨｨ ｨｪ06ｨｦ 06ｨｺｨｨ09ｨｨ ｨｺ0802ｨｬｨｪｨｨ07, 40 ｨｬ00/ｨｬ05), ｨｬ0203ｨｨ09ｨ 05ｨｨ ｨｨ ｨｨｨｪｨｺｨｮｨ ｨｨ080609ｨ - 86

3 1305ｨｨ 5 ｨｬｨｨｨｪ 0708ｨｨ ｨｺ06ｨｬｨｪｨ ｨｰｨｪ06ｨｦ ｨｰ02ｨｬ070208ｨ ｨｰｨｮ0802, ｨｨ0601ｨｨ ﾂ0209ｨｺｨｨ 0909ｨｰ080701ｨｨ09ｨ ｨ ｨｰ02ｨｬ 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ 0708ｨｨ ｨｬｨ ｨｺ09ｨｨｨｬｨ 05ｨｹｨｪ06ｨｦ 09ｨｺ ｨｰｨｨ, ｨｪ0609ｨｰｨｹ06 ｨｮｨ ｨｨ08ｨ 05ｨｨ 09ｨｮ070208ｨｪｨ ｨｰｨ ｨｪｨｰ, 0609ｨ 0106ｨｺ 09ｨｮ090702ｨｪ01ｨｨ080609ｨ 05ｨｨ ｨｬｨｺ05 08ｨ 09ｨｰ090608ｨ 02 (05ｨｰｨ ｨｪ0605 ｪ 25 %, ｨｨ ｨ ｨｪ0605 ｪ 25 %, NaCl ｪ 100 ｨｬM, 0608ｨｨ09-HCl ｪ 10 ｨｬM; ,0), 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ, ｨｪ0609ｨｰｨｹ06 06ｨｰｨ ｨｨ08ｨ 05ｨｨ 09ｨｮ070208ｨｪｨ ｨｰｨ ｨｪｨｰ, 0609ｨ 0106ｨｺ ｨｮ090702ｨｪ01ｨｨ080609ｨ 05ｨｨ 09 05ｨｰｨ ｨｪ060502, ｨｰｨｹ 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ ｨｨ ｨｪ0609ｨｰｨｹ06 ｨｮ01ｨ ｨｨ 09ｨｮ070208ｨｪｨ ｨｰｨ ｨｪｨｰ. 0209ｨ 0106ｨｺ ｨｮ03ｨｨ09ｨ 05ｨｨ ｨｪｨ ｨｮ ｨｰ02 ﾂ02ｨｪｨｨ02 15 ｨｬｨｨｨｪ, 0106ｨ ｨ ｨｨ 50 ｨｬｨｺ ｨｨ08ｨｮ06ｨｴ ｨ ｨｮ000208ｨ (0608ｨｨ09-HCl ｪ 10 ｨｬM, EDTA ｪ 1 ｨｬM; ,0) ｨｨ ｨ 05ｨｨ ｨｮｨｬ020802ｨｪｨｪ06ｨｬｨｮ 0909ｨｰ080701ｨｨ09ｨ ｨｪｨｨ06 ｨｪｨ ｨｰ02ｨｺ ｨｰ02 ﾂ02ｨｪｨｨ02 30 ｨｬｨｨｨｪ ｨｰ ｨ 08ｨ ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ ｨｨ 06ｨｰｨ ｨｨ08ｨ 05ｨｨ 09ｨｮ070208ｨｪｨ ｨｰｨ ｨｪｨｰ, ｨｨ04ｨ 0200ｨ ｨ 01ｨ ｨｪｨｨ07 06ｨｺｨｨ09ｨｨ ｨｺ0802ｨｬｨｪｨｨ ﾂｨｨｨｴ02ｨｪｨｪ04ｨｦ ｨ 08ｨ ｨｰ ｨｨ 0902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨｨｨ ｨｪｨｮｨｺ050206ｨｰｨｨ01ｨｪ ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ 06 ﾂｨｨｨｴ02ｨｪｨｪ04ｨｦ ｨ 08ｨ ｨｰ ｨｮ03ｨｨ05 ｨｬｨ ｨｰ08ｨｨ0202ｨｦ ｨ ｨｺ02ｨｨｨｨ ｨｮｨｪｨｨ ｨ 05ｨｹｨｪ04ｨｬｨｨ 0708ｨ ｨｦｨｬ0208ｨ ｨｬｨｨ ｨｺ 09ｨ 08ｨｨｨ ｨ 0205ｨｹｨｪ06ｨｬｨｮ ｨｮ ﾂｨ 09ｨｰｨｺｨｮ V4 0002ｨｪｨ 16S (F515 ｪ GTGCCAGCMGCCGCGGTAA, R806 ｪ GGATACVSGGGTATCTAAT) (7) ｨ ｨ ｨｪｨｨ02ｨｬ 0605ｨｨ0006ｨｪｨｮｨｺ050206ｨｰｨｨ01ｨｪ0401 ｨｨ0102ｨｪｨｰｨｨ00ｨｨｨｺｨ ｨｰ ｨｺｨ ｨｦ ｨ 04 ｨｨ 0905ｨｮ0302ｨ ｨｪ ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ, ｨｪ0206ｨ ｨｨｨｬ ｨｨ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ ｨｰ06ｨｺ0605ｨｮ 00Roche03 (010902ｨｦ02ｨ 08ｨｨ07) ｨｹ04ｨｮ02ｨｬ ｨ ｨｦｨｬ ｨ 0405ｨｨ 09ｨｺ06ｨｪ09ｨｰ08ｨｮｨｨ080609ｨ ｨｪ04 ｨｪｨ 0609ｨｪ ｨ ｨｪｨ 05ｨｨ04ｨ ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ ｨｺｨ ｨｺ ｨ ｨ ｨｺｨｰ0208ｨｨｨｦ, ｨｰｨ ｨｺ ｨｨ ｨ ｨｦ ｨｰ0609ｨｺｨｮ ｨ ｨｨ 0902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ ｨｨ05ｨｨ ｨｪｨ 0708ｨｨｨ GS Junior (00Roche03, ｨｦ02ｨ 08ｨｨ07) ｨ 09ｨｪ ｨｺ06ｨｬ02ｨｪ01ｨ 02ｨｨｨｨ ｨｨ ｨｨｨｰ ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ06ｨｦ ｨｨ0102ｨｪｨｰｨｨ00ｨｨｨｺｨ 02ｨｨｨｨ ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ ｨｪｨｨｨｰ0205ｨｹｨｪ06 ｨｰｨ ｨｺ ｨｹ040609ｨ 05ｨｨ09ｨｹ ｨ ｨ 0406ｨｦ 01ｨ ｨｪｨｪ0401 RDP (Ribosomal Database Project, ｨ 09ｨｪｨｨｨｰ0205ｨｹｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ04 ｨｬｨｨｨｺ0806ｨ ｨｪ ｨ ｨｴ0209ｨｰ ｨｪ0705ｨｨ 09 ｨｨ ｨｹ040609ｨ ｨｪｨｨ02ｨｬ ｨｮ0809ｨ VAMPS (Visualization and Analysis of Microbial Population Structure, ｨｰｨｮ0702ｨｪ ｨｪｨ 09ｨ ｨｦｨｰ ｨｬ06ｨｴｨｹ06 09ｨｨ09ｨｰ02ｨｬ04 VAMPS ｨｮ ﾂｨｨ05ｨｨ ｨｰｨ ｨ 05ｨｨ0204, ｨ ｨｴｨｨ02 04ｨｪｨ ﾂ02ｨｪｨｨ ｨｨ ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0401 ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ ｨｺｨ ｨｦ ｨ 02, ｨｪｨ 0609ｨｪ0609ｨ ｨｪｨｨｨｨ ｨｺ06ｨｰ ｨ 0405ｨｨ ｨｰ080602ｨｪ04 ｨｨｨｰ ｨｨｨ 0008ｨ ｨｬｨｬ ｨｬ07ｨｹ06ｨｰ0208ｨｪｨｮ06 06ｨ 08ｨ ｨ 06ｨｰｨｺｨｮ ｨｮ ﾂ02ｨｪｨｪ ｨｮ05ｨｹｨｰｨ ｨｰ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 ｨｪｨｮｨｺ050206ｨｰｨｨ01ｨｪ ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ, ｨｮ01ｨ 0502ｨｪｨｨ02 ｨｨ04 ｨｨ ｨｰｨ 09ｨ ｨｬ02ｨｰ06ｨｺ ｨｨ 0708ｨ ｨｦｨｬ ｨｮｨｴ0209ｨｰ ｨｨ ｨ 09ｨｪ06 ｨｬ02ｨｰ0601ｨｨ ﾂ0209ｨｺｨｨｨｬ 0802ｨｺ06ｨｬ02ｨｪ01ｨ 02ｨｨ07ｨｬ ｨｨ ｨｪｨｨｨｨ Ribosome Database Project (RDP) Pipeline (8) ｨｪ0705ｨｨ ｨ 09ｨｪｨｨ09ｨ ｨｪｨｨ02 ｨｪｨｮｨｺ050206ｨｰｨｨ01ｨｪ ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ, ｨｰ080602ｨｪｨｨ02 ｨｬｨ ｨｰ08ｨｨ ｨｪ02ｨｰｨｨ ﾂ0209ｨｺｨｨ01 08ｨ 0909ｨｰ0607ｨｪｨｨｨｦ ｨｨ ｨｺ05ｨ 09ｨｰ0208ｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ ｨｨｨｬ02ｨｪ02ｨｪｨｨ02ｨｬ ｨ ｨｨｨｰｨｬｨ 00average neighbor03. 02ｨ ｨｨ05ｨｨ ｨｺ05ｨ 0909ｨｨ00ｨｨｨｺｨ 02ｨｨ ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ ｨｪｨ OTU (Operational Taxonomic Unit) 09 ｨｨ ｨｹ040609ｨ ｨｪｨｨ02ｨｬ ｨｺ08ｨｨｨｰ0208ｨｨ07 97 % ｨｰ09ｨ ｨｮｨｴ0209ｨｰ090502ｨｪｨｨ07 00ｨ ｨｨｨｪｨ 08ｨｪ ｨｬ0705ｨｨｨｪ00ｨ 03 ｨｨ04 ｨｺｨ ｨｦ ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺｨｨ ｨ 08ｨ 05ｨｨ 0905ｨｮ ﾂｨ ｨｦｨｪｨｮ ｨ 0608ｨｺｨｮ ｨｨ ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ, ｨｪ0608ｨｬｨ 05ｨｨ04ｨｨ08ｨｮ07 ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺｨｨ ｨｮ06ｨｴ ｨ 09ｨｪ02ｨｪｨｨ07, ｨｨ ｨ 09ｨ 05ｨｨ 06ｨ ｨｲ0201ｨｨｨｪ02ｨｪｨｪ0402 ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺｨｨ 0706 ｨｺｨ ｨｬｨｮ ｨｨ ｨ 08ｨ ｨｪｨｪ ｨ ｨｺｨｰ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺｨｨ 0908ｨ 09ｨｪｨｨ09ｨ 05ｨｨ ｨｨ ｨｪｨｨｨｨ RDP 00LibCompare03 ( 06ｨ ｨｺｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ ｨ 0405ｨｨ ｨｬｨｨ080609ｨ ｨｪ04 ｨｰｨ ｨ 05ｨｨ ｨｺｨ ｨｬｨｮ 00ｨ ｨｺｨｰ0608ｨｮ, ｨ ｨｴｨｨ02 01ｨ ｨｪｨｪ ｨｪｨ ﾂｨｨｨｬ0609ｨｰｨｨ 08ｨ 0405ｨｨ ﾂｨｨｨｦ ｨｬ020301ｨｮ 0109ｨｮｨｬ07 ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺｨ ｨｬｨｨ, 06ｨ ｨｲ0201ｨｨｨｪ02ｨｪｨｪ04ｨｬｨｨ ｨｰ0902ｨｰ09ｨｰ09ｨｮ06ｨｴ02ｨｬｨｮ 00ｨ ｨｺｨｰ0608ｨｮ ｨｪｨｺｨｨ 04ｨｪｨ ﾂｨｨｨｬ0609ｨｰｨｨ 0905ｨｨ07ｨｪｨｨ07 00ｨ ｨｺｨｰ0608ｨ ｨｨ05ｨｨ 08ｨ 09ｨｪ04ｨｬ 0,05 ( ｨｺｨ 04ｨ ｨｰ0205ｨｨ ｨｰ ｨ ｨｪ02 06ｨｰ08ｨ 03ｨ 05ｨｨ 04ｨｪｨ ﾂｨｨｨｬ ｨ 0405ｨｨ ﾂｨｨｨｦ ｨｨ ｨｪｨ 06ｨ ｨｰ) ｨｮ05ｨｹｨｰｨ ｨｰ04. 02ｨ 08ｨ ﾂ ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ06ｨｬ ｨｬ ｨｰ ｨｨｨｬ ｨｨｨｬｨｨ ｨ 06ｨｰｨ ｨｨ08ｨ 05ｨｨ ｨ ｨｬｨｨ 05ｨｹｨｪｨ ｨｨ 0604ｨｨｨｬ06ｨｦ 0803ｨｨ, 09 ｨｨ ｨｰｨｺ0609ｨ ｨｪｨｨ02ｨｬ ｨｨ ｨ 0204, 09 ｨｮ ｨｨ ｨ ｨｰｨ ｨｨ ｨ 0204 ｨｪ (ｨｰｨ ｨ 05.). 87

4 1309ｨ 08ｨ ｨｺｨｰ0208ｨｨ09ｨｰｨｨｨｺｨ ｨｨ ｨ ｨｪｨｪ ｨ 08ｨ ﾂ0904 (09ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ04ｨｦ ｨｦ ｨｰ ｨｨｨｦ09ｨｺ ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ ｨ 0008ｨ 08ｨｪ ｨｮｨｪｨｨ ｨｨｨｰ02ｨｰｨ ｪ ｨｨｨｬ ｨｨｨｬｨｨ ｨ, ｨｺ09ｨ, ｨｨ06ｨｪｨｹ ｨ ) 01 ｧ爲 08ｨ 0402ｨ ｨｰｨｺ0609ｨ ｨｪｨｨ ｨ ｨｰ 08ｨｮ05ｨｹｨｰｨｮ08ｨ ﾁｨｨ ｨｪｨｮｨｺ050206ｨｰｨｨ01ｨｪ ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ ｨｹ ﾓ ｨｹ ｨｪ ﾓ ｨｪ ﾓ1 6ﾓ1 0902ｨｪ ﾓ1 0902ｨｪ ﾓ1 6ﾓ ｨｹ ﾓ ｨｹ ｨｨｨｬ02 ﾂｨ ｨｪｨｨ ｨｨ 006ﾓ103 ｪ ｨｰ0902ｨｰ09ｨｰ0902ｨｪｨｪ06 ｨｪｨ 05ｨｨ ﾂｨｨ02 ｨｨ 06ｨｰ09ｨｮｨｰ09ｨｰ09ｨｨ ｨｦ09ｨｰ09ｨｮ06ｨｴ ｨ ｨｺｨｰ0608ｨ ｨｨ ｨ ｨｪｨｨ02 00ｨｨ05 (08) ｨｨ (09) ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ ｨ 08ｨ 0402ｨ ｨｬ 0706 ﾂ0904 ( ｨ ｨｪｨｪ04ｨｬ ｨｺ ｨｨ ｨｨｨｰ0205ｨｹｨｪ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺ 0002ｨｪｨ 16S ): 1 ｪ 09 ｨｨ ｨｰｨｺ0609ｨ ｨｪｨｨ02ｨｬ ｨｨ ｨ 0806ｨｰ06ｨｬ, 2 ｪ ｨ ｨｰ06ｨｬ, 3 ｪ ｨ ｨ ｨｰｨ, 4 ｪ 09 ｨｨ ｨｰｨｺ0609ｨ ｨｪｨｨ02ｨｬ ｨ ｨ ｨｰｨ ( ｨｹ06); 5 ｪ 09 ｨｨ ｨｰｨｺ0609ｨ ｨｪｨｨ02ｨｬ ｨｨ ｨ ｨｰ06ｨｬ, 6 ｪ ｨ ｨｰ06ｨｬ, 7 ｪ ｨ ｨ ｨｰｨ, 8 ｪ 09 ｨｨ ｨｰｨｺ0609ｨ ｨｪｨｨ02ｨｬ ｨ ｨ ｨｰｨ ( ｨｹｨｪ06ｨｬ) (09ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ04ｨｦ ｨｦ ｨｰ ｨｨｨｦ09ｨｺ ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ ｨ 0008ｨ 08ｨｪ ｨｮｨｪｨｨ ｨｨｨｰ02ｨｰｨ ｪ ｨｨｨｬ ｨｨｨｬｨｨ ｨ, ｨｺ09ｨ, ｨｨ06ｨｪｨｹ ｨ ) ｨｰｨ ｨ ｨｴ0209ｨｰ09ｨ ｨ 0405ｨｨ 06ｨ ｨｪｨ 08ｨｮ0302ｨｪ ｨｰｨ 09ｨｨｨｰ0205ｨｨ 19 00ｨｨ05 ｨｬｨｨｨｺ-

5 ｨ ｨｪｨｨ04ｨｬ0609 (08ｨｨ09. 1, 08), ｨｨ ｨｺ06ｨｰ ｨ ｨｨ ｨｰｨ ｨｨ ｨｪ02ｨ 0605ｨｹ03ｨｮ (06ｨｰ % 09 04ｨ 09ｨｨ09ｨｨｨｬ0609ｨｰｨｨ 06ｨｰ 06ｨ 08ｨ 0402ｨ ). 0308ｨｨ 05ｨｰ06ｨｬ ｨｮ02ｨｰ 06ｨｰｨｬ02ｨｰｨｨｨｰｨｹ 09ｨｪｨｨ0302ｨｪｨｨ02 ﾂｨｨ0905ｨ ｨ ｨｦ 09 06ｨ 08ｨ 0402ｨ ｨ ｨｬ02ｨｪｨｪ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0806ｨｦ 0604ｨｨｨｬ06ｨｦ 0803ｨｨ ｨｨ ｨ ｨ ｨｺｨｰ0208ｨｨｨｦ ｨｪｨ ｨｨｨ ｨｨ0806ｨｺ06 06ｨｺｨ 04ｨ 05ｨ 09ｨｹ ｨｰｨ ｨｪｨ 00ｨｨ05ｨ Proteobacteria 09 09ｨｺ05ｨ 0106ｨｬ ｨ ｨｴ0209ｨｰ ｨｺ %, 04ｨ ｨｪ02ｨｦ ｨ 05ｨｨ Actinobacteria (06ｨｺ %), Acidobacteria (5 %) ｨｨ Firmicutes (5 %) ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609, ｨｪ02 ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ08ｨｮ02ｨｬ ｨｨ0504, ｨｰｨ ｨ 0708ｨｨｨｬ0208ｨｪ % ｨｪ06 ｨｰｨ ｨｺ ｨｨｨｰｨｹ ｨｪ0209ｨｺ0605ｨｹｨｺ06 00ｨｨ05, ｨｪｨ ｨｺ06ｨｰ ｨ ｨｴ0209ｨｰ ｨｨ010601ｨｨ050609ｨｹ 06ｨｺ % ｪ 05ｨｰ06 Crenarcheota, Verrucomicrobia, Bacteroidetes ｨｨ Gemmatimonadetes. ﾁｨｨ090502ｨｪｨｪ0609ｨｰｨｹ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609 ｨｨ ｨｰｨ 05ｨｹｨｪ ｨｨ ｨ ｨｴ0209ｨｰ09ｨ 01 ｨｪ ｨｰｨｨ00ｨ 05ｨ 1 % ｨｬ ｨｨ05ｨｨ 06ｨｺ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609 (09ｨｬ. 08ｨｨ09. 1, 09), 0708ｨｨ ﾂ02ｨｬ ｨ 0605ｨｹ03ｨｮ ｨｨ04 ｨｪｨｨ01 (06ｨｺ %) ｨｰｨ ｨｨ ｨｰ02, ｨｺ06ｨｰ ｨ ｨｰ0205ｨｹｨｪ0609ｨｰｨｨ ｨｪ02 ｨｮ01ｨ ｨｹ ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ080609ｨ ｨｰｨｹ ｨ. 0308ｨｨ 08ｨ 0909ｨｬ06ｨｰ0802ｨｪｨｨｨｨ ｨ ｨｴ0209ｨｰ09ｨ ｨｪｨ ｨｮ080609ｨｪ ｨ 09 00ｨｨ0502 Proteobacteria 0106ｨｬｨｨｨｪｨｨ080609ｨ 05ｨｨ ｨｰｨ 09ｨｨｨｰ0205ｨｨ Rhizobium (ｨｨ ｨ ｨｪｨｨ02 09ｨ 08ｨｹｨｨ080609ｨ ｨ %), Sphingomonas sp., Pseudomonas sp., ｨ ｨｰｨ ｨｺ0302 ｨｪ02 ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ08ｨｮ02ｨｬ ｨ ｨｰｨ 09ｨｨｨｰ0205ｨｨ ｨｺ05ｨ 0909ｨ Proteobacteria ｨ 08ｨ 0402ｨ ｨ ｨｬｨｨ 0604ｨｨｨｬ06ｨｦ 0803ｨｨ ｨｪｨｰ 08ｨｨ0406ｨ ｨｨｨｦ ﾂ ｨｨ05ｨｹｨｪ06 09ｨ 08ｨｹｨｨ080609ｨ ｨ ｨｪｨｨ ｨｨｨｬ06ｨｦ 0803ｨｨ ｨ ｨｰ ｨｨ090601ｨｨ0506 ｨｺ ｨｮ090205ｨｨ ﾂ02ｨｪｨｨ06 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｨ 08ｨｨ0406ｨ ｨｨｨｦ, 09 ｨｰ ｨｬ07 ｨｺｨ ｨｺ 0502ｨｪ ｨｪ02 06ｨｺｨ ｨ 05 ｨｰｨ ｨｺ ｨｨ07ｨｪｨｨ07 ｨｪｨｨ 09 ｨｮ ｨｨ ｨ ｨｰｨ, ｨｪｨｨ ｨ 0204 ｨｪ ｨｬ ｨｬ0603ｨｪ06 06ｨｰｨｬ02ｨｰｨｨｨｰｨｹ ｨ ｨｺｨｮ06 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｹ 08ｨｨ0406ｨ ｨｨｨｦ ｨ ｨｰ02, ﾂｨｰ ｨｪ02 06ｨ ｨｲ0709ｨｪｨｨｨｬ06, ｨｺ0605ｨｹｨｺｨｮ 0601ｨｪ06ｨｦ ｨｨ04 ｨｺｨｮ05ｨｹｨｰｨｮ08 09 ｨｪ02ｨｬ ｨ 0405 ｨｺ ｨｨ0502 Actinobacteria ｨ 0605ｨｹ03ｨｮ ｨ ｨｪｨｨｨｬｨ 05ｨｨ ｨｪ02ｨｨ ｨｰｨｪ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609 ｨｨ ｨｬ02ｨｦ09ｨｰ09 Acidimicrobidae ｨｨ Actinobacteridae, ｨ ｨｰｨ ｨｺ ｨ ｨｬ02ｨｰｨｪ04ｨｦ 09ｨｺ05ｨ 01 09ｨｪ0609ｨｨ05ｨｨ Arthrobacter sp. ( ｨ ｨｬｨｨ 05ｨｹｨｪｨ ｨ ｨｪｨｨ02 06ｨｺｨ 04ｨ ｨｹ , ﾂ02ｨｬ ｨ ｨｬｨｨ 0604ｨｨｨｬ06ｨｦ 0803ｨｨ), Solirubrobacter sp., Terrabacter sp. ｨｨ Nocardioides sp ｨｨ Acidobacteria 0106ｨｬｨｨｨｪｨｨ080609ｨ 05ｨｨ ｨｰｨ 09ｨｨｨｰ0205ｨｨ GP1, GP16, GP3, GP4 ｨｨ GP ｨ 08ｨ ｨ ｨ 08ｨ ｨｺｨｰ0208ｨｪ06 06ｨ ｨｴ ｨｪｨｨ0302ｨｪｨｨ02 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｨ Acidobacteria 0708ｨｨ ｨｨ ｨｰｨｺ0609ｨ ｨｪｨｨｨｨ 0706 ﾂ ｨ ｨｺ 06ｨｰｨｬ02 ﾂｨ 06ｨｰ C.L. Lauber ｨ 09ｨｰ. (9), ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ04 ｨｨ04 00ｨｨ0504 Acidobacteria ｨ ｨｪ04 ｨｺ 06ｨ ｨｨｨｰｨ ｨｪｨｨ ｨｨ ｨ ｨｺｨｨ ｨｪｨ ﾂ02ｨｪｨｨ0701 ph, ｨｨ 05ｨｰｨｨｨｬ ｨｬ0603ｨｪ06 06ｨ ｨｲ0709ｨｪｨｨｨｰｨｹ 09ｨｪｨｨ0302ｨｪｨｨ02 ｨｨ01 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｨ 09 06ｨ 08ｨ 0402ｨ ｨｨ ｨｰｨｺ0609ｨ ｨｪｨｨ02ｨｬ 0706 ﾂ ｨｹ03ｨｨｨｪ09ｨｰ ｨｰｨ 09ｨｨｨｰ020502ｨｦ 00ｨｨ0504 Firmicutes ｨｰｨ ｨｨ ｨｪ02 ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ｨ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ04 ｨｨ04 ｨｺ05ｨ Bacilli ｨｨ Clostridia, ｨ ｨｰｨ ｨｺ Paenibacillus sp. ｨｨ Bacillus sp. 08ｨｪｨ 05ｨｨ04 01ｨ ｨｪｨｪ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ ｨｺｨ 04ｨ 05, ﾂｨｰ ﾂ0902ｨｪｨｪ0602 ｨｬｨｨｨｺ0806ｨ ｨｪ ｨ ｨｴ0209ｨｰ ｨ 05ｨ 01ｨ 02ｨｰ 06 ﾂ02ｨｪｨｹ ｨｪ06ｨｦ 09ｨｰ08ｨｮｨｺｨｰｨｮ0806ｨｦ, 0202 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 06ｨｰ 06ｨ 08ｨ 0402ｨ ｨｺ 06ｨ 08ｨ 0402ｨｮ ｨｪ ｨ 06 ﾂ0209ｨｨ01ｨｪ04, ｨｰ06ｨｬｨｮ ｨｪｨｨｨｺ05ｨ ｨｪ0206ｨ ｨｨｨｬ0609ｨｰｨｹ ｨｮ ﾂｨｨｨｰｨｹ ｨｮ ﾂ02ｨｪｨｪ04ｨｦ ｨｬｨ ｨｰ0208ｨｨｨ 05, ﾂｨｰ06ｨ ｨｨｨｰｨｹ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ04, ｨｪｨ ｨｨｨ ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0402 ｨｺ 0102ｨｦ09ｨｰ09ｨｨ ｨｪｨｪ ｨｺ ｨｨ ﾂ0209ｨｺ ｨ ｨｺｨｰ0608ｨ ｨｰ06ｨｬ ｨｨ ｨ ｨｪｨｨｨｨ ｨｬ ｨｨ05ｨｨ ｨｨ ｨｹ040609ｨ ｨｰｨｹ ｨｬ02ｨｰ ｨ ｨｨｨｪｨ 08ｨｪ ｨｬ0705ｨｨｨｪ00ｨ 03, 0708ｨｨｨｪ02ｨｨ07 ｨｺ06ｨｰ ｨ ｨｨ09ｨ ｨｪ ｨｹ040609ｨ ｨｪｨｪ04ｨｦ ｨｨ05 ｨｪｨ ｨｬ ｨｨｨｰｨｹ 0008ｨｮ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609, ｨｪｨｪ0402 ｨｨ05ｨｨ ｨｪ ｨｪｨｪ ｨｨ07ｨｪｨｨ ｨ 08ｨ ｨｪｨｪ0401 ｨｪｨ ｨｬｨｨ 05ｨｺ ｨｨ ﾂ0209ｨｺｨｨ01 00ｨ ｨｺｨｰ (ｨｺｨｨ090506ｨｰｨｪ0609ｨｰｨｹ 0706 ﾂ0904, ｨ ｨｰ0201ｨｪｨｨｨｺｨ ｨｨ ｨ 02ｨｬｨ 07 ｨｺｨｮ05ｨｹｨｰｨｮ08ｨ ). 06ｨｪｨｰ ｨｪ06 06ｨｰｨｬ02ｨｰｨｨｨｰｨｹ, ﾂｨｰ06 09 ｨｺ0605ｨｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬ 06ｨｰｨｪ060302ｨｪｨｨｨｨ 0008ｨｮ0707ｨ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609, ｨｨ04ｨｬ02ｨｪ0706ｨｴｨｨ ｨｨ07ｨｪｨｨ02ｨｬ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ ｨ ｨｺｨｰ , 06ｨｺｨ 04ｨ 05ｨ 09ｨｹ 0708ｨｨｨｬ0208ｨｪ06 08ｨ 09ｨｪｨ 0008ｨｮ ｨｪ02 ｨｨ04ｨｬ02ｨｪ0706ｨｴｨｨ ｨｦ09ｨｰ09ｨｨ02ｨｬ 05ｨｰｨｨ01 00ｨ ｨｺｨｰ (08ｨｨ09. 2). 0201ｨｪｨ ｨｺ06 05ｨｰｨｨ 0008ｨｮ ｨｨ05ｨｹｨｪ06 08ｨ 0405ｨｨ ﾂｨ 05ｨｨ09ｨｹ 0706 ｨｺｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ ｨｰｨ 09ｨｮ: 0008ｨｮ0707ｨ, ｨｪｨｪｨ ｨｨ07ｨｪｨｨ06, ｨ 0405ｨ ｨ ｨｪ ｨｪｨ. 06ｨ ｨｺｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ, ｨｬ0603ｨｪ06 ｨｮｨｰ ｨ ｨｰｨｹ, ﾂｨｰ ｨｦ09ｨｰ09ｨｨ ｨ 02ｨｰ0907 ｨｪ02 06 ﾂ02ｨｪｨｹ ｨ 0605ｨｹ03ｨ 07 ﾂｨ 09ｨｰｨｹ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609, ｨ ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｹ 0108ｨｮ00ｨｨ ｨｰｨ 02ｨｰ0907 ｨ ｨｨ05ｨｨ ｨｬ02ｨｪ

6 1309ｨｰｨ ｨ ｨｨ05ｨｹｨｪ06ｨｦ. 04ｨｨ (%) 09ｨｨ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ ｨ 08ｨ 0402ｨ ﾂ0904, ｨｪ ｨｪｨｪ0401 (ｨ ) ｨｨ ｨｪｨｪ0401 (ｨ ) 0905ｨｨ07ｨｪｨｨ06 ｨｨ ｨｮ02ｨｬ ｨ ｨｺｨｰ ( ｨ ｨｪｨｪ04ｨｬ ｨｺ ｨｨ ｨｨｨｰ0205ｨｹｨｪ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺ 0002ｨｪｨ 16S ): I, II ｨｨ III ｪ ｨｰ0902ｨｰ09ｨｰ0902ｨｪｨｪ06 ｨｨ ｨｰｨｺ0609ｨ ｨｪｨｨ02, ｨ ｨｰ ｨｨ ｨ 02ｨｬｨ 07 ｨｺｨｮ05ｨｹｨｰｨｮ08ｨ (09ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ04ｨｦ ｨｦ ｨｰ ｨｨｨｦ09ｨｺ ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ ｨ 0008ｨ 08ｨｪ ｨｮｨｪｨｨ ｨｨｨｰ02ｨｰｨ ｪ ｨｨｨｬ ｨｨｨｬｨｨ ｨ, ｨｺ09ｨ, ｨｨ06ｨｪｨｹ ｨ ) ｨｰｨ 09ｨｨｨｰ0205ｨｨ ｨｰ ｨｨ05 ｪ Acidobacteria, Actinobacteria, Proteobacteria ｨ 0405ｨｨ ｨｬｨｪ ﾂｨｨ090502ｨｪｨｪ ｨ 08ｨ 0402ｨ 01 ｨｨ ｨｪ ｨｨ07ｨｪｨｨ ｨｨ ｨｮ02ｨｬ ｨ ｨｺｨｰ ｨｪｨｰ ｨｪ06 06ｨｰｨｬ02ｨｰｨｨｨｰｨｹ 00ｨｨ05ｨｮ Gemmatimonadetes (10): ｨｴｨｨｨｦ 09 ｨｪ ｨｨ01 Gemmatimonas sp. 06ｨｺｨ 04ｨ ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ04ｨｬ ｨｺ ｨｦ09ｨｰ09ｨｨ ｨ ｨｺｨｰ ｨｨ 08ｨ 0909ｨｬｨ ｨｰ08ｨｨ09ｨ ｨｰｨｹ 0905ｨｨ07ｨｪｨｨ02 05ｨｺ ｨｨ ﾂ0209ｨｺｨｨ01 00ｨ ｨｺｨｰ ｨｪｨ ｨ ｨ ｨｺｨｰ0208ｨｨｨｦ, ｨｬ0603ｨｪ ｨｨｨｰｨｹ ｨｨｨｪｨｰ ｨｪ ｨ ｨｺ06ｨｪ06ｨｬ0208ｨｪ0609ｨｰ02ｨｦ (08ｨｨ09. 3). 06ｨ ｨｺ, Rizobium sp. ｨｨ Gemmatimonas sp. ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ04 ｨｺ ｨｬ ｨｰ0802ｨｬ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ04ｨｬ ｨｪｨ ｨｬｨｨ 00ｨ ｨｺｨｰ0608ｨ ｨｬ (ｨｨ ｨｰｨｺ0609ｨ ｨｪｨｨ02, ｨ ｨｰ, ｨｺｨｮ05ｨｹｨｰｨｮ08ｨ 08ｨ 09ｨｰ02ｨｪｨｨｨｦ). 0005ｨｨ07ｨｪｨｨ ｨｰ ｨ ｨｺｨｰ ｨｪ04 ｨｰｨ ｨｺ ｨ 0405ｨｨ ﾂｨｪ ｨｰｨ 09ｨｨｨｰ0205ｨｨ 00ｨｨ0504 Acidobacteria ｨｬ06ｨｰ0807 ｨｪｨ ｨｰ06, ﾂｨｰ ｨｪｨｨ02, ｨｺｨ ｨｺ ｨｮ ｨｰｨｬ02 ﾂｨ ｨｹ, ﾂｨｨｨｰｨ 06ｨｰ ｨ ｨｺｨｨ0905ｨｮ ｨｮ 06ｨ ｨｨｨｰｨ ｨｪｨｨ07 (9), 09ｨｨ01ｨｪ06, ﾂｨｰ06 ｨｪ ｨｰｨ 09ｨｨｨｰ0205ｨｨ 05ｨｰ06ｨｦ 00ｨｨ ｨｨ05ｨｨ ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0609ｨｰｨｹ ｨｺ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ06 ｨｺｨｨ090506ｨｰｨｪ0609ｨｰｨｨ 0706 ﾂ ｨｹｨｺ06 ｨｮ Gp1 ｨｨ Gp2 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｹ 09 06ｨ 08ｨ 0402ｨ ｨｨ ｨｰｨｺ0609ｨ ｨｪｨｨ02ｨｬ ｨｮｨｬ02ｨｪｨｹ03ｨ 05ｨ 09ｨｹ, 0609ｨｰｨ 05ｨｹｨｪ0402 ｨ 02ｨｨ0106ｨ ｨ ｨｺｨｰ0208ｨｨｨｨ 06ｨｺｨ 04ｨ 05ｨｨ09ｨｹ ｨｮ09ｨｰ06ｨｦ ﾂｨｨ0904ｨｬｨｨ ｨｺ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ ｨｬ02 ｨｰ060006, 09ｨｰ06ｨｨｨｰ 06ｨｰｨｬ02ｨｰｨｨｨｰｨｹ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｨ Sphingomonas sp., Streptomyces sp. ｨｨ Nocardioides sp., ｨｪｨ ｨｺ06ｨｰ ｨｨ0705ｨｨ 05ｨｨ03ｨｹ 00ｨ ｨｺｨｰ060804, ｨ ｨｪｨｪ ｨ 02ｨｬ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0806ｨｦ ｨｨ ｨ ｨｰ06ｨｬ. 0604ｨｬ02ｨｪ02ｨｪｨｨ ｨｪ0204ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪ06 09ｨｺｨ 04ｨ 05ｨｨ09ｨｹ ｨｪｨ 05ｨｰｨｨ01 09ｨｨ01ｨ 01 ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609. Arthrobacter sp. ｨ 0405 ﾂｨｮ0909ｨｰ09ｨｨｨｰ020502ｨｪ ｨｺ ｨｦ09ｨｰ09ｨｨ ｨ ｨｰｨ ｨｨ ｨ ｨｴｨｨ09ｨ 02ｨｬ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ ｨｰｨｺ0609ｨ ｨｪｨｨ02 09ｨｪｨｨ03ｨ 0506 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｹ ｨ ｨ ｨｺｨｰ0208ｨｨｨｦ ｨｨ Solirubrobacter, Paenibacillus, Bradyrhizobium. 04ｨｨ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609, ｨｪｨｪ ｨｨ07ｨｪｨｨ06 ｨｨ ｨｰｨｺ0609ｨ ｨｪｨｨ07, ｨ ｨｰｨ ｨｨ ｨ 02ｨｬ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0804 (ｨｰ0802ｨｮ000605ｨｹｨｪｨｨｨｺｨｨ ｪ ｨｹ, ﾂ0208ｨｪ0402 ｨｺ08ｨｮ03ｨｺｨｨ ｪ 0502ｨｪ), ｨｺ06ｨｰ ｨ 0405ｨｨ ｨｪ04 09 ｨｨ ｨ ｨｪｨｪ ｨ 08ｨ 0402ｨ ﾂ ｨ ｨｪｨｪ04ｨｬ ｨｺ ｨｨ ｨｨｨｰ0205ｨｹｨｪ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺ 0002ｨｪｨ 16S ｨｬ06ｨｮ000605ｨｹｨｪｨｨｨｺｨ ｨｬｨｨ 06ｨｰｨｬ02 ﾂ02ｨｪ , ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0402 ｨｺ ｨｬ ｨｰ0802ｨｬ 00ｨ ｨｺｨｰ0608ｨ ｨｬ (09ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ04ｨｦ ｨｦ ｨｰ ｨｨｨｦ09ｨｺ ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ ｨ 0008ｨ 08ｨｪ ｨｮｨｪｨｨ ｨｨｨｰ02ｨｰｨ ｪ ｨｨｨｬ ｨｨｨｬｨｨ ｨ, ｨｺ09ｨ, ｨｨ06ｨｪｨｹ ｨ ). 0005ｨｨ07ｨｪｨｨ ｨ ｨｰｨ 06ｨｺｨ 04ｨ 05ｨ 09ｨｹ ｨｪｨ ｨｰｨ ｨｰ06 ﾂｨｪ06 06ｨ 03ｨｨ08ｨｪｨ ｨｮ0707ｨ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609, ｨｨ ｨｺ06ｨｰ Nocardioides sp., Spartobacteria sp., Hyphomicrobium sp., Streptomyces sp. ｨｨ ｨｹ09ｨｺ ｨｦ09ｨｰ0902ｨｪｨｪｨ 07 ｨｺｨｮ05ｨｹｨｰｨｮ08ｨ (09 ｨｪｨ 0302ｨｬ 0905ｨｮ ﾂｨ ｨｪ ｨｨ 0604ｨｨｨｬｨ ｨｹ) 0905ｨｨ0702ｨｰ ｨｪｨ Nocardioides sp., Sphi- 90

7 13ngomonas sp., Streptomyces sp. ｨｨ ｨｪｨ ｨｺ ｨｨｨｺ 0907ｨｨ0906ｨｺ ｨｨ ｨｰ0201 ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609, ｨｺ06ｨｰ ｨｪ02 ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ04 ｨｺ ｨｮｨｺｨ 04ｨ ｨｪｨｪ06ｨｬｨｮ 00ｨ ｨｺｨｰ0608ｨｮ ｨｨ ｨ ｨ ｨｺｨｰ0208ｨｨｨｦ, ｨｮ09ｨｰ06ｨｦ ﾂｨｨ ｨｺ ｨｦ09ｨｰ09ｨｨ ｨｰ ｨ ｨｺｨｰ , ｨｬ0603ｨｪ ｨｨｨｰｨｹ ｨｰｨ ｨｺｨｨ01 ｨｨ ｨｰｨｪ ｨｰｨ 09ｨｨｨｰ020502ｨｦ 0706 ﾂ0902ｨｪｨｪ06ｨｦ ｨｬｨｨｨｺ0806ｨ ｨｨ06ｨｰ04, ｨｺｨ ｨｺ Pseudomonas sp., Bacillus sp., Rubrobacter sp. 06ｨ ｨｺｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ, ｨｨ ｨ ｨｪｨｪ0402 ｨｪｨ ｨｬｨｨ 00ｨ ｨｺｨｰ ｨｺｨ ｨ 05ｨｨ 09ｨｨ05ｨｹｨｪ ｨｦ09ｨｰ09ｨｨ02 ｨｪｨ ｨｰｨ 09 ｨｬｨｨｨｺ0806ｨ ｨｪ ｨ ｨｴ0209ｨｰ09ｨ, 01ｨ 09ｨ ｨｨｨｬｨｮｨｴ0209ｨｰ ｨ 0409ｨｨｨｰｨｨｨｨ ｨｪｨｪ04ｨｬ 0008ｨｮ0707ｨ ｨｬ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609, ｨｨｨｬ0206ｨｴｨｨ01 ｨｰ02 00ｨｨ04ｨｨ ｨｨ ﾂ0209ｨｺｨｨ ｨ 02ｨｪｨｪ0609ｨｰｨｨ, ｨｺ06ｨｰ ｨｰ ｨｨｨｬ 04ｨ ｨｪｨｨｨｬｨ ｨｰｨｹ 04ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪｨｮ06 ﾂｨ 09ｨｰｨｹ 05ｨｺ ｨｨ ﾂ0209ｨｺ06ｨｦ ｨｪｨｨ03ｨｨ. 05ｨｮｨｴ0209ｨｰ09ｨｮ02ｨｰ ｨｨ03ｨｪｨ 0703 ﾂｨ 09ｨｰｨｹ ｨｬｨｨｨｺ0806ｨ ｨｪ ｨ ｨｴ0209ｨｰ09ｨ 0706 ﾂ0904, 09 ｨｪ ｨｰ ｨｪｨ ｨｨｨ ｨｬｨｪ ﾂｨｨ090502ｨｪｨｪ ﾂ0902ｨｪｨｪ0401 ｨ ｨ ｨｺｨｰ0208ｨｨｨｦ, ｨｰｨ ｨｺｨｨ02 ｨｺｨ ｨｺ Rhizobium, Arthrobacter (09ｨｬ. 08ｨｨ09. 3). 06ｨｰｨ ｨ ｨｺｨｰｨｨ09ｨｪｨ ｨｮ0707ｨ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609 ｨｬ060302ｨｰ ｨ 04ｨｰｨｹ 04ｨ 0102ｨｦ09ｨｰ090609ｨ ｨｪｨ ｨｪ0609ｨｪ ﾂ090606ｨ 08ｨ ｨ ｨｰ0205ｨｹｨｪ ｨ 01 ｨｨ ｨｰ06ｨｬｨｮ ｨ ﾂｨｮｨｰｨｺ ｨ 00ｨｨ08ｨｮ02ｨｰ ｨｪｨ 09ｨｬ02ｨｪｨｮ ｨｮ ｨｨｨｦ 06ｨｺ08ｨｮ03ｨ 06ｨｴ02ｨｦ ｨｰ ｨｬ ﾂ ｨｰ0607ｨｪｨｪ ｨ ｨｪ0702ｨｰ ｨｮ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609, ｨｺ06ｨｰ060804ｨｦ 0905ｨｮ03ｨｨｨｰ ｨｨ09ｨｰ06 ﾂｨｪｨｨｨｺ06ｨｬ ｨｰ0607ｨｪｨｪ ｨｺｨ ﾂ0209ｨｰ0902ｨｪｨｪ ｨｨ ｨｺ0605ｨｨ ﾂ0209ｨｰ0902ｨｪｨｪ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ ｨｰｨ 09ｨ ｨｬｨｨｨｺ0806ｨ ｨｪ ｨ ｨｴ0209ｨｰ09ｨ ｨｬ06ｨｰ0807 ｨｪｨ 06ｨ ｨｪｨ 08ｨｮ0302ｨｪｨｪ0402 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ ｨｰ08ｨｮｨｺｨｰｨｮ ﾂ0902ｨｪｨｪ06ｨｦ ｨｬｨｨｨｺ0806ｨ ｨｨ06ｨｰ04, ｨｬ0603ｨｪ06 09ｨｺｨ 04ｨ ｨｰｨｹ, ﾂｨｰ06 05ｨｰ ｨ ｨｴ0209ｨｰ0906, ｨｮ09ｨｰ06ｨｦ ﾂｨｨ ｨｺ 0102ｨｦ09ｨｰ09ｨｨ06 ｨｬｨｪ0600ｨｨ01 09ｨｪ0203ｨｪｨｨ01 00ｨ ｨｺｨｰ (ｨｺｨｨ090506ｨｰｨｪ0609ｨｰｨｹ, ｨｮ ｨｨ07, ｨ 09ｨ 02ｨｬ ｨｰ09ｨｨ ｨｨｨｬ02ｨｪ02ｨｪｨｨ07 ｨ ｨｰ0201ｨｪｨｨｨｺｨｨ) ｨ ｨｺ0506 ﾂ02ｨｪｨｨ02 06ｨｰｨｬ02ｨｰｨｨｨｬ, ﾂｨｰ ｨｨｨｰｨｹ 0008ｨｮ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609, ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0402 ｨｺ 0102ｨｦ09ｨｰ09ｨｨ06 04ｨ 01ｨ ｨｪｨｪ ｨｺ ｨｨ ﾂ0209ｨｺ ｨ ｨｺｨｰ0608ｨ, ｨｮ01ｨ 02ｨｰ0907 ｨｪ ｨ ｨｰ06ｨｬｨｮ ｨｪｨ ｨｬｨｨ ｨ ｨ 0609ｨ ｨｪ ｨｬ02ｨｰ ｨ ｨｨｨｪｨ 08ｨｪ ｨｬ0705ｨｨｨｪ00ｨ 03, ｨｴｨｨｨｦ ｨ ｨｮｨ 06ｨｺ06 ｨ ｨｪｨ 05ｨｨ04ｨｨ080609ｨ ｨｰｨｹ ｨｨ 09ｨｨ09ｨｰ02ｨｬｨ ｨｰｨｨ04ｨｨ080609ｨ ｨｰｨｹ 01ｨ ｨｪｨｪ ｨｨ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ ﾂ0902ｨｪｨｪ06ｨｦ ｨｪ ｨｨ ｨ ｨｰｨｹ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 09 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｨ ｨｺ06ｨｪｨｺ0802ｨｰｨｪ0401 ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ ｨｨ07ｨｪｨｨ02ｨｬ 05ｨｺ ｨｨ ﾂ0209ｨｺｨｨ01 00ｨ ｨｺｨｰ ｨｬ ｨｪ02 ｨｬ02ｨｪ0202, ｨｨｨｪ060001ｨ ｨｪ020706ｨｪ07ｨｰｨｪ06, 0706 ﾂ02ｨｬｨｮ ｨｰｨ ｨｨ05ｨｨ ｨｨｨｪｨ ｨｮ0707ｨ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609 ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪｨ 05ｨｨｨ 06 ｨｪ02 ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪｨ ｨｺ 0102ｨｦ09ｨｰ09ｨｨ06 05ｨｺ ｨｨ ﾂ0209ｨｺ ｨ ｨｺｨｰ0608ｨ, ｨｺ0605ｨｹｨｺｨｮ ｨｪｨｺｨｨ 05ｨｰ ｨｰ0802ｨ ｨｮ02ｨｰ ｨｪｨ ｨｪｨｨ02 00ｨｨ04ｨｨ ｨｨ ﾂ0209ｨｺｨｨ ｨ 02ｨｪｨｪ0609ｨｰ02ｨｦ ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ ｨ ｨｰｨｹ 00ｨｮｨｪｨｺ02ｨｨ06ｨｪｨ 05ｨｹｨｪｨｮ06 09ｨｰ08ｨｮｨｺｨｰｨｮ08ｨｮ ｨｪ0202, ﾂ02ｨｬ ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺｨｮ06, ｨｰ06ｨｬｨｮ ｨｪ0206ｨ ｨｨｨｬ ｨ ｨｪｨｨ ﾂｨｨｨｰｨｹ ｨｺ08ｨｮ00 ｨｨ04ｨｮ ﾂｨ 02ｨｬ0401 ｨｬｨｨｨｺ ｨ ｨｪｨｨ04ｨｬ0609 ｨｨ 08ｨ 0909ｨｬｨ ｨｰ08ｨｨ09ｨ ｨｰｨｹ ｨｰ0605ｨｹｨｺ06 ｨｰ02 ｨｨ04 ｨｪｨｨ01, ｨｺ06ｨｰ ｨ 00ｨｨ08ｨｮ06ｨｰ ｨｪｨ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ02 00ｨ ｨｺｨｰ ｨｺ08ｨｮ03ｨ 06ｨｴ02ｨｦ ｨｰ0601ｨｨｨｺｨ ｨｪｨｪ ｨｪｨ ｨｬｨｨ ｨｨ ｨ ｨｪｨｨ ｨｰｨ ｨｰ 0906ｨ 06ｨｦ ｨｦ 03ｨ ｨｰ06ｨｬ ｨｪｨ 0708ｨ ｨｪｨｨｨｨ, ｨｰｨ ｨｺ ｨｺｨ ｨｺ 01ｨ 02ｨｰ ｨｬ0603ｨｪ0609ｨｰｨｹ ｨｰｨｪ0209ｨｰｨｨ ｨｺ06ｨｪｨｺ0802ｨｰｨｪ0402 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 09 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ06ｨｦ 09ｨｰ08ｨｮｨｺｨｰｨｮ ｨ ｨｴ0209ｨｰ09ｨ ｨｦ09ｨｰ09ｨｨ02ｨｬ ｨｪｨｪ ｨｺ ｨｨ ﾂ0209ｨｺ ｨ ｨｺｨｰ0608ｨ. 06ｨｰｨ ｨｺ, ｨｪｨ 0609ｨｪ ｨ ｨｪｨ 05ｨｨ04ｨ 01ｨ ｨｪｨｪ ｨｺ ｨｨ ｨｨｨｰ0205ｨｹｨｪ ｨｨ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ ｨｪ ｨ ｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ04 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ06ｨｦ 09ｨｰ08ｨｮｨｺｨｰｨｮ0804 ｨｬｨｨｨｺ0806ｨ ｨｪ ｨ ｨｴ0209ｨｰ09ｨ 09 09ｨｨ09ｨｰ02ｨｬ02 ｨｬｨｪ ｨｰｨｪ ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ ｨｰｨ ｨｨｨｦ09ｨｺ ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ ｨ 0008ｨ 08ｨｪ ｨｮｨｪｨｨ ｨｨｨｰ02ｨｰｨ ｪ ｨｨｨｬ ｨｨｨｬｨｨ ｨ ( ｨｹｨｬｨｨ 09ｨ 08ｨｨｨ ｨｪｨｰｨ 01, 06ｨｰ08ｨ 03ｨ 06ｨｴｨｨ01 05ｨｺ ｨｨ ﾂ0209ｨｺ ｨｨ07ｨｪｨｨ02 08ｨ 0405ｨｨ ﾂｨｪ ﾂ02ｨｰｨ ｨｪｨｨｨｦ ｨｰ ｨ ｨｰ0201ｨｪｨｨ ﾂ0209ｨｺｨｨ01 00ｨ ｨｺｨｰ ｪ ｨ 02ｨｬ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0804, ｨ ｨｰｨ ｨｨ ｨｨ ｨｰｨｺ0609ｨ ｨｪｨｨ ﾂ0904). 0308ｨｨ ｨ ｨｪｨ 05ｨｨ0402 ｨｨ 09ｨｨ09ｨｰ02ｨｬｨ ｨｰｨｨ04ｨ 02ｨｨｨｨ ｨｮ05ｨｹｨｰｨ ｨｰ ｨｨ ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ ﾂ0902ｨｪｨｪ06ｨｦ ｨ ｨ 0609ｨ ｨｪ ｨｬ02ｨｰ ｨ ｨｨｨｪｨ 08ｨｪ ｨｬ0705ｨｨｨｪ00ｨ 03, ﾂｨｰ06 01ｨ ｨｬ0603ｨｪ0609ｨｰｨｹ ｨｨｨｰｨｹ 0008ｨｮ ｨ ｨｪｨｨ04ｨｬ0609, ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0401 ｨｺ 0102ｨｦ09ｨｰ09ｨｨ06 04ｨ 01ｨ ｨｪｨｪ ｨ ｨｺｨｰ ｨｺｨ 04ｨ ｨｪ06, ﾂｨｰ ｨ 00ｨ 02ｨｬ04ｨｦ ｨｰ ｨｰｨｪ0209ｨｰｨｨ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 09 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ06ｨｦ 09ｨｰ08ｨｮｨｺｨｰｨｮ0802 ｨｬｨｨｨｺ0806ｨ ｨｪ ｨ ｨｴ0209ｨｰ09ｨ ｨｪｨｪ04ｨｬ 09ｨｪ0203ｨｪｨｨｨｬ ｨｦ09ｨｰ09ｨｨ02ｨｬ ｨｨｨｦ09ｨｺｨｨｨｦ ｨｹ09ｨｺ ｨｦ09ｨｰ0902ｨｪｨｪ06ｨｦ ｨｬｨｨｨｺ0806ｨ ｨｨ ｨｨｨｨ ｨｹ010604ｨ ｨｺｨ 0102ｨｬｨｨｨｨ, ｨｨ07, ｨ ｨｪｨｺｨｰ-0302ｨｰ0208ｨ ｨｮ0800 ｪ03ｨｮ03ｨｺｨｨｨｪ, ｨ 0205ｨｹ09ｨｺ060006, 3, ｨｰｨｮ07ｨｨ05ｨ ｨ ｨｺ02ｨｨ06 28 ｨｨ06ｨｪ ｨ 91

8 13Sel ｯskokhozyaistvennaya biologiya [Agricultural Biology], 2013, 01 5, pp THE MAIN TRENDS IN DYNAMICS OF SOIL MICROBIOM DURING A LONG-TERM FIELD EXPERIMENT AS INDICATED BY HIGH THROUGHPUT SEQUENCING THE 16S-rRNA GENE LIBRARIES 92 V.A. Dumova, E.V. Pershina, Ya.V. Merzlyakova, Yu.V. Kruglov, E.E. Andronov All-Russian Research Institute of Agricultural Microbiology, Russian Academy of Agricultural Sciences, 3, sh. Podbelskogo, Saint Petersburg-Pushkin, Russia, doumova@gmail.com Received June 28, 2012 doi: /agrobiology eng Acknowledgements: Supported by Ministry of Education and Science of the Russian Federation, and Russian Foundation for Basic Research Abstract A biodiversity of soil microbial communities now is being widely studied. Since 1912, the influence of different agrotechnical factors on soil is being examined in the stationary field experi-ment in the Moscow Agricultural Academy. We investigated an effect of different combination of three factors, i.e. plants (flax and winter rye), crop rotation and soil acidity, to the soil microbiom. To estimate a biodiversity, the throughput sequencing was used. Also an original approach, the bi-nary sampling, was suggested to analyze the data of throughput sequencing. It was shown the number of microorganisms which changed under the influence of studied factor is approximately equal to those staying unchanged. But a taxonomic composition of these two groups differed, and the impacted group was more uniform. Thus, we can conclude that the less part of microorganism is sensitive to the acting factors, and the rest ones keep their number unchanged, more or less. The taxonomic groups of microorganisms, sensitive to each of studied factors, were revealed. The binary sampling, suggested herein, allows finding out a relation between an action of some ecological fac-tor and varying taxonomic structure of microbial population. Keywords: soil microbiom, high throughput sequencing. REFERENCES 1. Pace N.R. Mapping the tree of life: progress and prospects. Microbiology and Molecular Biology Reviews, 2009, 73(4): Tyson G.W., Banfield J.F. Cultivating the uncultivated: a community genomics perspective. Trends in Microbiology, 2005, 9(13): Kakirde K.S., Parsley L.C., Liles M.R. Size does matter: application-driven approaches for soil metagenomics. Soil Biol. Biochem., 2010, 42: Lombard N., Prestat E., Elsas J.D.V., Simonet P. Soil-specific limitations for access and analysis of soil microbial communities by metagenomics. FEMS Microbiol. Ecol., 2011, 78: Wooley J.C., Ye Y. Metagenomics: Facts and artifacts, and computational challenges. Journal of Computer Science and Technology, 2010, 1(25): Mardis E.R. Next-generation DNA sequencing methods. Annual Review of Genomics and Human Genetics, 2008, 9: Bates S.T.D., Berg-Lyons J.G., Caporaso W.A. et al. Examining the global distribution of dominant archaeal populations in soil. ISME Journal, 2010, 5: Cole J.R., Wang Q., Cardenas E. et al. The Ribosomal Database Project: improved alignments and new tools for rrna analysis. Nucl. Acids Res., 2009, 37: D141-D Lauber C.L., Strickland M.S., Bradford M.A., Fierer N. The influence of soil properties on the structure of bacterial and fungal communities across land-use types. Soil Biol. Biochem., 2008, 40: Zhang H., Sekiguchi Y., Hanada S. et al. Gemmatimonas aurantiaca gen. nov., sp. nov., a gramnegative, aerobic, polyphosphate-accumulating micro-organism, the first cultured representative of the new bacterial phylum Gemmatimonadetes phyl. nov. International Journal of Systematic and Evolutionary Microbioly, 2003, 53(4): ｨ ｨｮ ﾂｨｪ ｨ 08ｨ ｨｪｨｨ07 THE 2 nd PLANT GENOMICS CONGRESS (12-13 May, 2014, London, UK) Attracting experts working in next generation sequencing, plant genomics/sciences, epigenetics, bioinformatics and data management, the conference will continue to examine the latest NGS platforms and technologies suitable for progressing plant based research as well as tools to enable successful analysis. 06ｨｪ000608ｨｬｨ 02ｨｨ07: