Tsitologiya. 57 (4) : 260–268

Transcription

1 ｨｬ 57, ﾁ , ｨ, ｨｬ0205ｨｹ07ｨｪ0609ｨ, ｨｨｨｪ060008ｨ 0109ｨｺｨ 07, 2, 3, * ﾁｨｨ ﾂ0209, ｨｮ0804ｨ ｨｺ0609ｨ, ｨｨ08ｨｨ ﾂ02ｨｪｨｺ06, ｨ 02ｨｪｨｺ06, ｨｮｨｴ ｨ ｨｮ ﾂｨｪ06-ｨｨ ｨ ｨｰ0205ｨｹ09ｨｺｨｨｨｦ ｨｨｨｪ09ｨｰｨｨｨｰｨｮｨｰ 09ｨｨ08ｨｮ ｨｨｨｨ ｨｨｨｬ ｨ ｨｪ060909ｨｺ ｨｨｨｪｨｨ09ｨｰ020809ｨｰ09ｨ ｨ ｨ ｨｪ02ｨｪｨｨ , ｨｺ09ｨ ; ｨｰ0208ｨ ｨｮ080009ｨｺｨｨｨｦ ｨｨｨｪ09ｨｰｨｨｨｰｨｮｨｰ ｨｪ06ｨｦ 00ｨｨ04ｨｨｨｺｨｨ ｨｨｨｬ ｨｪ09ｨｰｨ ｨｪｨｰｨｨｨｪ0609ｨ, ｨｮ08 ﾂｨ ｨｰ060909ｨｺｨｨｨｦ ｨｨｨｪ09ｨｰｨｨｨｰｨｮｨｰ03, 05ｨ ｨｪｨｺｨｰ-0302ｨｰ0208ｨ ｨｮ0800, ｨｨ 3 08ｨ ｨ ｨ ｨｨ0600ｨｨ04ｨｨｨｺｨｨ ｨｰ0208ｨ ｨｮ080009ｨｺ ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ ｨｨｨｰ0201ｨｪｨｨ ﾂ0209ｨｺ ｨｮｨｪｨｨ ｨｨｨｰ02ｨｰｨ ; * ｨｺｨｰ0806ｨｪｨｪ04ｨｦ ｨ : gvinogradskaya@mail.ru ｨ ｨｨ ｨｨ07ｨｪｨｨ02 ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺｨ 0106ｨｺ090608ｨｮｨ ｨｨ02ｨｨｨｪｨ ｨｪｨ 00ｨｨｨ 0806ｨ 05ｨ 09ｨｰ04 ﾂ ｨｺｨ, ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ｨｨｨｰ06ｨｬ0200ｨ ｨｨ08ｨｮ0906ｨｬ ｨｰｨｨ090608ｨ ｨｺ060904ｨｦ ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺ 0106ｨｺ090608ｨｮｨ ｨｨ02ｨｨｨｪ ( ) 01ｨ 08ｨ ｨｺｨｰ0208ｨｨ04ｨｮ02ｨｰ ｨｺ06ｨｦ ｨｰ06ｨｺ09ｨｨ ﾂｨｪ0609ｨｰｨｹ06, ｨ ｨｪ02ｨｪｨｨ ｨ ｨ 06ｨｰ02 03ｨｨ04ｨｪ02ｨｪｨｪ06 09ｨ 03ｨｪ ｨ ｨｪ ｨ ｨｪｨｪ ｨｦ09ｨｰ09ｨｨｨｨ ｨｪｨ ｨｪ0608ｨｬｨ 05ｨｹｨｪ0402 ｨｺ0502ｨｰｨｺｨｨ ｨ ｨｪｨｨ04ｨｬｨ ｨｪ ｨｰｨ ｨｰ06 ﾂｨｪ ｨ 00ｨ 06ｨｰ, ﾂｨｰ ｨｨｨｪ01ｨｮ02ｨｨ08ｨｮ02ｨｰ 00ｨｨｨ 0205ｨｹ 08ｨ ｨｺ ｨｺ0502ｨｰ06ｨｺ 07ｨｮｨｰ02ｨｬ ｨ ｨｰ0604ｨ, 0601ｨｪｨ ｨｺ ｨｪｨｨ ｨｨ ｨ 0205ｨｺｨ 0873, ﾂ0502ｨｪｨ 0902ｨｬ02ｨｦ09ｨｰ09ｨ 0607ｨｮ ｨｮ ｨ 0853, 0708ｨ ｨｺｨｰｨｨ ﾂ0209ｨｺｨｨ 06ｨｰ09ｨｮｨｰ09ｨｰ09ｨｮ06ｨｰ ｨｰｨｪ06, ﾂｨｰ06 02ｨｨｨｰ06ｨｬ0200ｨ ｨｨ08ｨｮ09 (000000) ｨｨｨｪ01ｨｮ02ｨｨ08ｨｮ02ｨｰ ｨ ｨｪｨｰｨｨｨ ｨｰ0604ｨｪｨｮ ｨ ｨｬｨｬｨｮ, ｨｴｨｮ06 09ｨｨ08ｨｮ09ｨｮ 0609ｨｮｨｴ0209ｨｰ09ｨｨｨｰｨｹ ｨｨｨｺｨ 02ｨｨ ｨｪ06ｨｬｨ ｨｨｨ 0205ｨｨ ｨｺ0502ｨｰｨｺｨｨ-ｨｬｨｨ0302ｨｪｨｨ ｨｹ ｨｪｨ 09ｨｰ0607ｨｴ02ｨｦ 08ｨ ｨ 06ｨｰ ｨｰ060705ｨ 09 ｨｨ04ｨｮ ﾂ02ｨｪｨｨｨｨ 0102ｨｦ09ｨｰ09ｨｨ ｨｪｨ ｨｪ0608ｨｬｨ 05ｨｹｨｪ0402 ｨｺ0502ｨｰｨｺｨｨ, ｨ ｨｪｨ 05ｨｨ ｨ ｨｪ0609ｨｰｨｨ ｨ ｨｪｨｰｨｨｨ ｨｰ0604ｨｪ06ｨｦ ｨ ｨｬｨｬ04, 04ｨ 07ｨｮ09ｨｺｨ 02ｨｬ06ｨｦ , 06ｨｺｨ ｨ ｨｰｨｹ 0905ｨｨ07ｨｪｨｨ02 ｨｪｨ ｨｰ06ｨｺ09ｨｨ ﾂｨｪ0609ｨｰｨｹ ｨｨ ｨｪｨｺ02 ｨｮ ﾂｨ 09ｨｰｨｨ07 ｨ 0205ｨｺｨ 0873 ｨｨ ｨｨ ｨｬ ｨｮ050702ｨｨｨｨ 00ｨｨｨ 0205ｨｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0401 ｨｺ0502ｨｰ06ｨｺ ｨｦ09ｨｰ09ｨｨ02ｨｬ ﾂ02ｨｰ ｨｺ0502ｨｰ06ｨｺ 09 02ｨｨｨｰ ｨｨ ﾂ0209ｨｺｨｨ ｨ 08ｨ ｨｰｨ ｨｺｨ 04ｨ 05, ﾂｨｰ06 09 ｨｰ02 ﾂ02ｨｪｨｨ02 24 ﾂ ｨ 02ｨｰ 00ｨｨｨ 0205ｨｹ 06ｨｺ % 00ｨｨｨ 0806ｨ 05ｨ 09ｨｰ ｨｺ ｨｬｨ 08ｨｨ06ｨｪｨ ﾂ ｨｺｨ ( ﾁ) 09 ｨｺｨｮ05ｨｹｨｰｨｮ0802 in vitro, ｨ 0708ｨｨ 0906 ﾂ02ｨｰｨ ｨｪｨｪ06ｨｬ 0102ｨｦ09ｨｰ09ｨｨｨｨ ｨｨ ｨｨｨ 0205ｨｹ ｨｺ0502ｨｰ06ｨｺ ｨｮｨｬ02ｨｪｨｹ03ｨ 02ｨｰ ｨｰ060106ｨｬ TUNEL ｨｪ06, ﾂｨｰ06 ｨｺ0605ｨｨ ﾂ0209ｨｰ0906 ｨｺ0502ｨｰ06ｨｺ 09 08ｨ ｨ ｨｬｨｨ ｨｨ 0102ｨｦ09ｨｰ09ｨｨｨｨ ｨｪｨｨ03ｨ 02ｨｰ % (P < 0.05) 0708ｨｨ ｨｦ09ｨｰ09ｨｨｨｨ ｨｪｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0402 ｨｺ0502ｨｰｨｺｨｨ. 08ｨｪｨ 05ｨｨ04 ｨｬｨｨｨｰ06ｨｰｨｨ ﾂ0209ｨｺｨｨ01 00ｨｨ00ｨｮ ｨｨ05 ｨｪｨ ｨｺ ｨｪｨｨ02 ｨｺ0502ｨｰ06ｨｺ 09 ｨｬｨｨｨｰ ｨｦ09ｨｰ09ｨｨ02ｨｬ , ｨｰ060001ｨ ｨｺｨ ｨｺ 09 ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ｨｺ0502ｨｰｨｺｨ ｨ ｨｪ ｨｪｨｨ07 ｨｪ02 ｨｪｨ ｨ ｨ 05ｨｨ. 08ｨｪｨ 05ｨｨ04 ｨｬ ｨｮ01 ｨｨ ｨｬ ｨ 0205ｨｺｨ 0873 ( ｨｨ dnp73) 0706ｨｺｨ 04ｨ 05, ﾂｨｰ06 09 ｨｪ02ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0401 ｨｺ0502ｨｰｨｺｨ 01 05ｨｺ ｨｨ07 ｨｨ ｨｬ ﾂ02ｨｪｨｹ ｨｪｨｨ04ｨｺｨ 07, ｨｰ060001ｨ ｨｺｨ ｨｺ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ﾁ ｨｮ ｨｪｨｹ ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ ｨｨ ｨｬ ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪ ｨｪ, ｨｨ ｨ ｨｪｨ 08ｨｮ0302ｨｪｨ 05ｨｺ ｨｨ07 ｨｨ ｨｬ04 dn ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0401 ｨｺ0502ｨｰｨｺｨ 01, 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ , ｨｪ ｨｨ ｨｨ0506 ｨ 0506ｨｺｨ ｨｬｨｨｨｰ0604ｨ, ｨｪ06 ｨｪｨ ｨ ｨ 05ｨ 09ｨｹ ｨ ｨｺｨｰｨｨ09ｨ 02ｨｨ07 ｨｺｨ 0907ｨ 04 8, 9 ｨｨ 3, ｨｺ06ｨｰ0608ｨ ｨ 05ｨ 09ｨｹ 00ｨｨｨ 0205ｨｹ06 ｨｺ0502ｨｰ06ｨｺ, 0601ｨｪｨ ｨｺ06 ｨｪ02 09ｨｰ0605ｨｹ ｨｬｨ ｨｦ, ｨｺｨ ｨｺ 0708ｨｨ 0102ｨｦ09ｨｰ09ｨｨｨｨ ｨｮ ﾂ02ｨｪｨｪ ｨ ｨｪｨｪ ｨｰ 04ｨ ｨｺ0506 ﾂｨｨｨｰｨｹ, ﾂｨｰ ｨ ｨ ｨｰ ｨ 06ｨｴ ｨｦ09ｨｰ09ｨｨ ｨｪｨ ｨｪ0608ｨｬｨ 05ｨｹｨｪ0402 ｨｺ0502ｨｰｨｺｨｨ ｨｪ ｨｨｨｰｨｹ, ﾂｨｰ06 ｨｨｨｪ01ｨｮｨｺ02ｨｨ ｨｨ ｨｪｨｨ02 ｨｮｨｺ ﾂ02ｨｪｨｪ06ｨｦ ｨｨ ｨｬ ｨ 06ｨｰ ｨｮ ｨｨ ｨ ｨｰｨｰ02ｨｪｨｮｨ 02ｨｨｨｨ 0905ｨｨ07ｨｪｨｨ ｨｪｨ ｨｪｨｨ ｨｨ 00ｨｨｨ 0205ｨｹ ｨｪ0608ｨｬｨ 05ｨｹｨｪ0401 ｨｺ0502ｨｰ06ｨｺ ﾂ ｨ : 0106ｨｺ090608ｨｮｨ ｨｨ02ｨｨｨｪ, 00ｨｨｨ 0806ｨ 05ｨ 09ｨｰ04 ﾂ ｨｺｨ, 02ｨｨｨｰ06ｨｬ0200ｨ ｨｨ08ｨｮ09, ｨｨ ｨｬ ｨｨ dnp73 ｨ 0205ｨｺｨ ｨｨｨｪ07ｨｰ ｨｺ08ｨ ｨｴ02ｨｪｨｨ07: ｪ 0106ｨｺ090608ｨｮｨ ｨｨ02ｨｨｨｪ, ｪ 02ｨｨｨｰ06ｨｬ0200ｨ ｨｨ08ｨｮ09, ﾁ ｪ 00ｨｨｨ 0806ｨ 05ｨ 09ｨｰ ｨｺ ｨｬｨ 08ｨｨ06ｨｪｨ ﾂ ｨｺｨ ｪ ｨｰｨｨ090608ｨ ｨｺ060904ｨｦ ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺ, ｨ ｨｰ 0708ｨｨｨｬ02ｨｪ0702ｨｬ04ｨｦ ﾂ02ｨｪｨｨ07 03ｨｨ0806ｨｺ ｨｺｨｰ08ｨ 06ｨｪｨｺ ｨｨ ﾂ0209ｨｺｨｨ01 04ｨ ｨ ｨ ｨｪｨｨｨｦ ｨｮｨｴ0209ｨｰ0902ｨｪｨｪ04ｨｬ ｨｪ ｨｰｨ ｨｰｨｺ06ｨｬ ｨｰ ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪｨ 07 ｨｰ06ｨｺ09ｨｨ ﾂｨｪ0609ｨｰｨｹ, ｨｺ06ｨｰ0608ｨ ｨｨ090601ｨｨｨｰ ｨｺ ｨｪ02ｨｪｨｨ07ｨｬ 09 08ｨ ｨ 06ｨｰ ｨ ｨｨ 0702 ﾂ02ｨｪｨｨ, ｨｺ 0706ｨｰ ｨｮ01ｨ ｨｨ 0108ｨｮ00ｨｨｨｬ 07ｨ ｨｰ ｨｨ07ｨｬ. 00 ｨｪｨ 09ｨｰ0607ｨｴ ｨｬ ｨｮｨｰ0907 ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0402 ｨｨ ｨ ｨｪｨｨ07, ｨｪｨ 0708ｨ ｨｪｨｪ0402 ｨｪｨ 0706ｨｨ09ｨｺ ｨｰ09, 09ｨｪｨｨ03ｨ 06ｨｴｨｨ01 ｨｰ06ｨｺ09ｨｨ ﾂｨｪ0609ｨｰｨｹ ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺｨ ｨｨ ｨ 06ｨｴｨｨ01 ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0609ｨｰｨｹ ｨｺ ｨｪ02ｨｬｨｮ (Wang et al., 2014; Yu et al., 2014), ｨｺ06ｨｰ ｨｺｨ ｨｪ ｨｨ090205ｨｨ ｨｺ 04ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪ04ｨｬ ｨｮ ｨ ｨｬ. 0201ｨｪｨ ｨｨ ｨｨ ﾂｨｨｨｪ ｨｰ06ｨｨｨｰ 09 ｨｪ ｨｰｨ ｨｰ06 ﾂｨｪ06ｨｦ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ0609ｨｰｨｨ 0102ｨｦ09ｨｰ09ｨｨ ｨｪｨ ｨｪ020607ｨｮ ｨｺ0502ｨｰｨｺｨｨ 09 ｨｰｨｺｨ ｨｪ0701, 06ｨｺ08ｨｮ03ｨ 06ｨｴｨｨ ｨｮ010605ｨｹ, , 0702 ﾂ02ｨｪｨｨ ｨｨ ｨ ｨｪｨｨ04ｨｬ ｨｬ. 0604ｨｮ ﾂ02ｨｪｨｨ02 ｨｬ0201ｨ ｨｪｨｨ04ｨｬ ｨｰｨｨ ｨｮ ｨｦ09ｨｰ09ｨｨ07 ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺｨ 0708ｨｨ ｨｺ 04ｨ ｨｺ0506 ﾂ02ｨｪｨｨ06 06 ｨｰ06ｨｬ, ﾂｨｰ ｨｨｨｪ01ｨｮ02ｨｨ08ｨｮ02ｨｰ 00ｨｨｨ 0205ｨｹ ｨｺ0502ｨｰ06ｨｺ 07ｨｮｨｰ02ｨｬ ｨ ｨｰ0604ｨ 09 ｨｮ ﾂｨ 09ｨｰｨｨ02ｨｬ ｨ 0205ｨｺｨ 0853, 0601ｨｪｨ ｨｺ06 ｨｨｨｬ0206ｨｴｨｨ ｨ ｨｪｨｪ ｨｰｨｨ ﾂｨｨ0904, ｨ ｨｨ 0108ｨｮ00ｨｨ01 ｨ 0205ｨｺ ｨｰ ｨｬ02ｨｦ09ｨｰ09ｨ ｨｨｨｪ01ｨｮ02ｨｨ080609ｨ ｨｪｨｪ06ｨｬ ｨ ｨｰ ｨｪｨｨ07 ｨｪ02ｨｬｨｪ ﾂｨｨ090502ｨｪｨｪ04 (Minotti et al., 2004). 00 ｨｰ ｨｬ ｨｺｨ 04ｨ ｨｪ06, ﾂｨｰ06 ｨ ｨｺ 0873 ｪ ﾂ0502ｨｪ 0902ｨｬ02ｨｦ09ｨｰ09ｨ ｨｰｨｨ ｨｮ ｨ 0205ｨｺ ｪ ｨｮ ﾂｨ 09ｨｰ09ｨｮ02ｨｰ ｨｮ050702ｨｨｨｨ ｨｺ0502ｨｰ06 ﾂｨｪ ｨｪｨｨ07 ｨｨ 00ｨｨｨ 0205ｨｨ ｨｺ0502ｨｰ06ｨｺ, ｨｨｨｬ0202ｨｰ ｨｪ0209ｨｺ0605ｨｹｨｺ06 ｨｨ ｨｬ, ｨｺ06ｨｰ ｨｬ0600ｨｮｨｰ 06ｨｺｨ ｨ ｨｰｨｹ ｨｺｨ ｨｺ ｨ ｨｰ0604ｨｪ0602, ｨｰｨ ｨｺ ｨｨ 06ｨｪｨｺ060002ｨｪｨｪ ｨｦ09ｨｰ09ｨｨ ｨｰｨｪ06, ﾂｨｰ ﾂ ｨｺｨ, ｨ 01ｨ ｨｺ0502ｨｰｨｺｨｮ, ｨｨｨｪ01ｨｮ02ｨｨ08ｨｮ02ｨｰ ｨ ｨｪｨｰｨｨｨ ｨｰ0604ｨｪｨｮ ｨ ｨｬｨｬｨｮ, ｨｴｨｮ06 09ｨｨ08ｨｮ09ｨｮ 0609ｨｮｨｴ0209ｨｰ09ｨｨｨｰｨｹ ｨｨｨｺｨ 02ｨｨ

2 130005ｨｨ07ｨｪｨｨ02 ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺｨ 0106ｨｺ090608ｨｮｨ ｨｨ02ｨｨｨｪｨ ｨｪｨ 00ｨｨｨ 0806ｨ 05ｨ 09ｨｰ04 ﾂ ｨｺｨ, ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ｨｨｨｰ06ｨｬ0200ｨ ｨｨ08ｨｮ0906ｨｬ ｨｪ06ｨｬｨ ｨｨｨ 0205ｨｨ ｨｺ0502ｨｰｨｺｨｨ-ｨｬｨｨ0302ｨｪｨｨ ｨｰｨ ｨｨｨｪｨｰ ｨｪｨｨｨｰｨｹ, ｨ ｨｪｨ 05ｨｨ ｨ ｨｪｨｰｨｨｨ ｨｰ0604ｨｪｨ ｨ ｨｬｨｬｨ, 04ｨ 07ｨｮ09ｨｺｨ 02ｨｬｨ , 06ｨｺｨ ｨ ｨｰｨｹ 0905ｨｨ07ｨｪｨｨ02 ｨｪｨ ｨｰ06ｨｺ09ｨｨ ﾂｨｪ0609ｨｰｨｹ ｨｰｨｪ060302ｨｪｨｨｨｨ ｨｪ0608ｨｬｨ 05ｨｹｨｪ0401 ｨｺ0502ｨｰ06ｨｺ ｪ 00ｨｨｨ 0806ｨ 05ｨ 09ｨｰ0609 ﾂ ｨｺｨ, ｨ ｨｰｨ ｨｺ0302 ｨｺｨ ｨｺ ｨｮ ﾂｨ 09ｨｰｨｨ02 ｨ 0205ｨｺｨ 0873 ｨｨ ｨｨ ｨｬ ｨｮ050702ｨｨｨｨ ｨｺ0502ｨｰ06 ﾂｨｪ06ｨｦ 00ｨｨｨ 0205ｨｨ ｨｦ09ｨｰ09ｨｨ02ｨｬ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0401 ｨｺ0502ｨｰｨｺｨ ｨｰ0902ｨｰ04 ｨｪｨ ｨｰｨ ｨｪｨｪ ｨｪ07ｨｰ ｨｨｨｬ0206ｨｴｨｨ ｨｪｨｨ07 06 ｨｬ0201ｨ ｨｪｨｨ04ｨｬｨ ｨｦ09ｨｰ09ｨｨ07 ｨｨ ｨｰ06ｨｺ09ｨｨ ﾂｨｪ0609ｨｰｨｨ , ｨ ｨｰｨ ｨｺ ｨｬ0600ｨｮｨｰ 09 08ｨ 0408ｨ ｨ 06ｨｰｨｺ02 ｨｪ ｨｰ08ｨ ｨｰ0200ｨｨｨｦ 0502 ﾂ02ｨｪｨｨ07 06ｨｪｨｺ0604ｨ ｨ ｨ ｨｪｨｨｨｦ. 00ｨ ｨｰ0208ｨｨｨ 05 ｨｨ ｨｬ02ｨｰ0601ｨｨｨｺｨ ｨｰ ｨｺ ｨｨ. 02ｨ ｨｲ02ｨｺｨｰ06ｨｬ ｨｨ ｨ ｨｪｨｨ ｨｮ03ｨｨ05ｨｨ 01ｨｨ070506ｨｨ01ｨｪ ｨｨｨ 0806ｨ 05ｨ 09ｨｰ ｨｺ ｨｬｨ 08ｨｨ06ｨｪｨ ﾂ ｨｺｨ ( ﾁ), ｨｮ ﾂ02ｨｪｨｪ0402 ｨｨ04 ｨｺ ｨｺ02ｨｨｨｨ ｨｺ0502ｨｰ06 ﾂｨｪ ｨｨｨｪｨｨｨｦ 01ｨ ｨｮ ﾂｨｪ06-ｨｨ ｨ ｨｰ0205ｨｹ09ｨｺ ｨｨｨｪ09ｨｰｨｨｨｰｨｮｨｰｨ 09ｨｨ08ｨｮ ｨｨｨｨ ｨｨｨｬ ｨ ｨｪ060909ｨｺ ｨｨｨｪｨｨ09ｨｰ020809ｨｰ09ｨ ｨ ｨ ｨｪ02ｨｪｨｨ (000609ｨｺ09ｨ ). 08ｨｮ05ｨｹｨｰｨｮ08ｨｮ ｨｺ0502ｨｰ06ｨｺ ｨ ｨｴｨｨ09ｨ 05ｨｨ ｨｪｨ , ｨ ｨｴ02ｨｦ 10 % 05ｨｬｨ 08ｨｨ06ｨｪｨ 05ｨｹｨｪ06ｨｦ ｨｰ ﾂｨｹ02ｨｦ ｨｰｨｺｨｨ (060605), 2 ｨｬ00 L-0005ｨｮｨｰｨ ｨｬｨｨｨｪｨ ｨｨ 50 ｨｬｨｺ00/ｨｬ ｨｪｨｰｨ ｨｬｨｨ02ｨｨｨｪｨ (03ｨ ｨｪ06ｨｺ06, ｨｨ07). 00ｨｨ08ｨｮ ｨｹ040609ｨ 05ｨｨ ｨｪ09-03ｨｰｨ ｨｬｨｬ AD 169, 0506ｨ 0204ｨｪ ｨｰｨ ｨｪｨｪ04ｨｦ ｨｬ ｨｬｨ ｨｪｨｮ0505ｨｹ (D. Emanuel, ). 00ｨｨ08ｨｮ09 08ｨ 04ｨｬｨｪ0603ｨ 05ｨｨ 07ｨｮｨｰ02ｨｬ 07ｨ 0909ｨｨ080609ｨ ｨｪｨｨ07 ｨｪｨ ｨｺｨｮ05ｨｹｨｰｨｮ0802 ｨｺ0502ｨｰ06ｨｺ ﾁ. 08ｨｨｨ 0806ｨ 05ｨ 09ｨｰ04 04ｨ 08ｨ 03ｨ 05ｨｨ 09 ｨｨｨｪ0002ｨｺ02ｨｨ06ｨｪｨｪ06ｨｦ ｨｬｨｪ ｨｰ0902ｨｪｨｪ0609ｨｰｨｹ06 3 ｪ /ｨｺ ｨ 02ｨｨ06 09ｨｨ08ｨｮ09ｨ ｨｨ05ｨｨ 09 ｨｰ02 ﾂ02ｨｪｨｨ02 1 ﾂ 0708ｨｨ 37 縺05, 04ｨ ｨｰ02ｨｬ 09ｨｨ08ｨｮ ｨ ｨｴｨｮ06 03ｨｨ01ｨｺ0609ｨｰｨｹ 06ｨｰｨｬ0409ｨ 05ｨｨ ｨｨ 09ｨｪ0609ｨｨ05ｨｨ ｨｨ09ｨ 06ｨｴｨｮ ｨｮ ｪ % ｨ 08ｨ ｨ 06ｨｰｨｺｨ ﾁ ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺ06ｨｬ ｨｰｨｺｨｨ ｨ 03ｨｨ09ｨ 05ｨｨ 09 ｨｺｨｮ05ｨｹｨｰｨｮ08ｨ 05ｨｹｨｪ ｨ ｨｺ06ｨｪ04 (Coster, ) ｨｨ05ｨｨ ｨｮｨｪ06 ﾂｨｪ ｨ ｨｪ0205ｨｨ (Corning, ) 09 ｨｺ06ｨｪ0202ｨｪｨｰ08ｨ 02ｨｨｨｨ 200 ｨｰ0409. ｨｺ0502ｨｰ06ｨｺ ｨｪｨ 1 ｨｬ ｨｨ ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ 05ｨｨ 09 ｨｰ02 ﾂ02ｨｪｨｨ02 48 ﾂ 0708ｨｨ 37 縺05 09 ｨ ｨｰｨｬ % C 27 H 29 NO 11 (Sigma, ) 09ｨｪ0609ｨｨ05ｨｨ 09 ｨｺｨｮ05ｨｹｨｰｨｮ08ｨｮ ﾁ 09 ｨｺ06ｨｪ0202ｨｪｨｰ08ｨ 02ｨｨｨｨ 5 ｨｬｨｺ00/ｨｬ05 ｨｨ ｨｨｨｪｨｺｨｮｨ ｨｨ080609ｨ 05ｨｨ 8 ｨｨ05ｨｨ 24 ﾂ. 0202ｨｦ09ｨｰ09ｨｨ ｨｪｨ ﾁ ｨｪｨｨ09ｨ 05ｨｨ ｨ ｨｺ02ｨｨ0701, 0607ｨｨ09ｨ ｨｪｨｪ0401 ｨｪｨｨ ｨ 08ｨ ｨ 06ｨｰｨｺｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0401 ｨｺ0502ｨｰ06ｨｺ ｨｰｨｺｨｨ ﾁ ｨ 03ｨｨ09ｨ 05ｨｨ 09 ｨｺ06ｨｪ0202ｨｪｨｰ08ｨ 02ｨｨｨｨ 200 ｨｰ0409. ｨｺ0502ｨｰ06ｨｺ 09 1 ｨｬ ｨｨ ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ 05ｨｨ 09 ｨｰ02 ﾂ02ｨｪｨｨ02 24 ﾂ, ｨｺｨ ｨｺ 0607ｨｨ09ｨ ｨｪ ｨ ｨｰ02ｨｬ 04ｨ 08ｨ 03ｨ 05ｨｨ ｨｨｨｪ0002ｨｺ02ｨｨ06ｨｪｨｪ06ｨｦ ｨｬｨｪ ｨｰ0902ｨｪｨｪ0609ｨｰｨｹ06 3 ｪ /ｨｺ05. ﾁ ﾂ ｨ 08ｨ 0302ｨｪｨｨ07 09 ｨｺｨｮ05ｨｹｨｰｨｮ08ｨｮ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0401 ｨｺ0502ｨｰ06ｨｺ 0106ｨ ｨ ｨｨ ｨｺ06ｨｪ0202ｨｪｨｰ08ｨ 02ｨｨｨｨ 5 ｨｬｨｺ00/ｨｬ05 ｨｨ ｨｨｨｪｨｺｨｮｨ ｨｨ080609ｨ 05ｨｨ 09 ｨｰ02 ﾂ02ｨｪｨｨ ｨｮ06ｨｴｨｨ01 24 ﾂ ﾂｨ ｨｦ ｨｨｨｪｨｺｨｮｨ ｨ 02ｨｨｨｨ ( ﾂ ﾂ ｨ 08ｨ 0302ｨｪｨｨ ) ｨｺ0502ｨｰｨｺｨｨ 06ｨｰｨｬ0409ｨ 05ｨｨ ｨｨ ｨｨ05ｨｨ 0908ｨ 09ｨｪｨｨｨｰ0205ｨｹｨｪ0602 ｨｨ04ｨｮ ﾂ02ｨｪｨｨ02 ｨｺｨｮ05ｨｹｨｰｨｮ ﾁ ｨｬ06ｨｴｨｹ06 ｨｬ02ｨｰ , 0607ｨｨ09ｨ ｨｪｨｪ0401 ｨｪｨｨ ｨｨｨｰ06ｨｰ06ｨｺ09ｨｨ ﾂ0209ｨｺ ｨｦ09ｨｰ09ｨｨ ｨｪｨｨ09ｨ 05ｨｨ 07ｨｮｨｰ02ｨｬ ﾂ02ｨｰｨ ﾁ 09 ｨｺ06ｨｪｨｰ080605ｨｹｨｪ06ｨｦ (ｨ ) ｨｨ ｨｰｨｪ06ｨｦ (06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ06ｨｦ ) ｨｮ050702ｨｨ ｨｰ ｨｺ0502ｨｰｨｺｨｨ ｨ 03ｨｨ09ｨ 05ｨｨ ｨｮｨｪ06 ﾂｨｪ ｨ ｨｪ0302ｨｰ ｨｺ080609ｨｪ04ｨｬｨｨ 09ｨｰ02ｨｺ05ｨ ｨｬｨｨ ｨｨ 0106ｨ ｨ ｨｨ ｨｺ06ｨｪ0202ｨｪｨｰ08ｨ 02ｨｨｨｨ 5 ｨｬｨｺ00/ｨｬ ﾂｨ ｨｦ ｨｨｨｪｨｺｨｮｨ ｨ 02ｨｨｨｨ ｨｺ0502ｨｰｨｺｨｨ ｨｬ0409ｨ 05ｨｨ ｨ ｨｰｨｪ ｨｬ ｨ ｨｮ ｨｬ ( ) (080509), 04ｨ ｨｰ02ｨｬ 00ｨｨｨｺ09ｨｨ080609ｨ 05ｨｨ ｨ ｨ ｨｰｨｪ04ｨｬ ｨｬ02ｨｰｨ ｨｪ060506ｨｬ 20 ｨｬｨｨｨｪ 0708ｨｨ ｨC20 縺05. 08ｨｨｨｺ09ｨｨ080609ｨ ｨｪｨｪ0402 ｨｺ0502ｨｰｨｺｨｨ 06ｨｺ08ｨ 03ｨｨ09ｨ 05ｨｨ 0002ｨｬｨ ｨｰ06ｨｺ09ｨｨ05ｨｨｨｪ06ｨｬ 08ｨ 08ｨ ﾂ ﾂｨｨ (090608ｨｨ ｨ , ｨｨ07) ｨｨ ﾂｨｨｨｰ0409ｨ 05ｨｨ ｨｬｨｨｨｺ080609ｨｺ060706ｨｬ (AxioCamMRc 5, Zeizz, ｨｬｨ ｨｪｨｨ07) ﾂｨｨｨｰ0409ｨ 05ｨｨ ｨｺ0502ｨｰｨｺｨｨ ｨｪ02 ｨｬ02ｨｪ0202 ﾂ02ｨｬ ｨｪｨｨ07 ｨｨ ｨｮ05ｨｹｨｰｨ ｨｰ ｨ 03ｨ 05ｨｨ 09 ｨｺ0605ｨｨ ﾂ0209ｨｰ0902 ｨｺ0502ｨｰ06ｨｺ ｨｪｨ ｨｪｨｨ07 ｨｬｨｨｨｺ080609ｨｺ0607ｨ (06ｨｺ , 06ｨ ) ｨｺ0502ｨｰ06ｨｺ ｨｰｨｪ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ ｨ 03ｨ 05ｨｨ ｨｪｨｰｨ 01 06ｨｰ ｨｺ06ｨｪｨｰ080605ｨｹｨｪ06ｨｦ ｨｮ050702ｨｨｨｨ. 02ｨ ｨｪｨ 08ｨｮ0302ｨｪｨｨ02 ｨｬｨ 08ｨｺ ｨ ｨｰ0604ｨ ｨｨ ｨ ｨｮｨｰ0600 ｨ 00 ｨｨ ｨｨ. 00 ｨｺｨ ﾂ0209ｨｰ0902 ｨｬｨ 08ｨｺ ｨ ｨｰ0604ｨ ｨｨ ｨ ｨｺｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｪ0402 ｨｺｨ 0907ｨ , 8 ｨｨ 9,09 ｨｺｨ ﾂ0209ｨｰ0902 ｨｬｨ 08ｨｺ0208ｨ ｨ ｨｮｨｰ0600ｨ 00ｨｨｨｨ ｪ ｨ ｨｺ LCP3A/B ｨｨ05ｨｨ ｨｨｨｬｨｬｨｮｨｪ0602ｨｨｨｰ0601ｨｨｨｬｨｨ ﾂ0209ｨｺｨｨｨｦ ｨ ｨｪｨ 05ｨｨ ｨ ｨｺ02ｨｨｨｨ ｨｪ ｨｬ06ｨｦ ｨｨｨｬｨｬｨｮｨｪ060005ｨｮ ｨｪ02ｨｨｨｨ in situ 09 ｨｨ ｨｹ040609ｨ ｨｪｨｨ02ｨｬ ｨｨ00ｨｨ ﾂ0209ｨｺｨｨ01 ｨ ｨｪｨｰｨｨｨｰ ｨｰｨｺｨｨ 00ｨｨｨｺ09ｨｨ080609ｨ 05ｨｨ ｨ ｨｪｨｪ04ｨｬ ｨｬ02ｨｰｨ ｨｪ060506ｨｬ, ｨｺｨ ｨｺ ｨｮｨｺｨ 04ｨ ｨｪ , ｨｬ0409ｨｺｨｨ 00ｨｨｨｺ09ｨｨ080609ｨ ｨｪｨｪ ｨ 08ｨ ｨｰ ｨｪｨ ｨｺ0502ｨｰｨｺｨｨ ｨｪｨ ｨｪ0609ｨｨ05ｨｨ 08ｨ 09ｨｰ ｨｨ00ｨｨ ﾂ0209ｨｺｨｨ01 ｨ ｨｪｨｰｨｨｨｰ , ｨ ｨｴｨｨｨｦ 1 % ｨ 04 ﾂｨｹ ｨｰ06 ﾂｨｪ ｨ 05ｨｹｨ ｨｮｨｬｨｨｨｪｨ (Sigma, ). 06ｨｪｨｺｨｮｨ ｨｨ080609ｨ 05ｨｨ 09 ｨｰ02 ﾂ02ｨｪｨｨ02 1 ﾂ 0708ｨｨ 37 縺 ｨｪｨｨ07 ｨｺｨ 0907ｨ 04 ｨｨ ｨｹ040609ｨ 05ｨｨ ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｺ ｨｺｨ 0907ｨ (ab52293, Abcam, ｨｨｨｺ06ｨ 08ｨｨｨｰｨ ｨｪｨｨ07) ｨｨ ｨｺｨ 0907ｨ (ab4052, Abcam, ｨｨｨｺ06ｨ 08ｨｨｨｰｨ ｨｪｨｨ07) 09 08ｨ ｨｪｨｨｨｨ 1 : 50 ｨｨ ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｺ ｨｺｨ 0907ｨ (ab32539, Abcam, ｨｨｨｺ06ｨ 08ｨｨｨｰｨ ｨｪｨｨ07) 09 08ｨ ｨｪｨｨｨｨ 1 : ｨｪｨｨ07 ｨｬｨ 08ｨｺ0208ｨ ｨ ｨｮｨｰ0600ｨ 00ｨｨｨｨ ｨｨ ｨｹ040609ｨ 05ｨｨ ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｺ ｨ 0205ｨｺｨｮ LC3A/B (ab58610, Abcam, ｨｨｨｺ06ｨ 08ｨｨｨｰｨ ｨｪｨｨ07) 09 08ｨ ｨｪｨｨｨｨ 1 : ｨｨｨｪｨｺｨｮｨ ｨ 02ｨｨｨｨ ｨｬｨｨ ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｬｨｨ ｨｺ0502ｨｰｨｺｨｨ ｨｬ0409ｨ 05ｨｨ ｨｨ ｨｨｨｪｨｺｨｮｨ ｨｨ080609ｨ 05ｨｨ ｨｰ060804ｨｬｨｨ ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｬｨｨ 09 ｨｰ02 ﾂ02ｨｪｨｨ02 30 ｨｬｨｨｨｪ 0708ｨｨ 37 縺 ｨｺｨ ﾂ0209ｨｰ ｨｰ ｨ ｨｪｨｰｨｨｨｰ0205 ｨｨ ｨｹ040609ｨ 05ｨｨ ｨｺ0604ｨｹｨｨ ｨ ｨｪｨｰｨｨｨｺ080605ｨｨ ﾂｨｹｨｨ ｨｨｨｺ0506ｨｪｨ 05ｨｹｨｪ0402 ｨ ｨｪｨｰｨｨｨｰ0205ｨ, ｨｺ06ｨｪｨｲ0600ｨｨ080609ｨ ｨｪｨｪ ｨｮ ｨｬ06ｨｬ (ab98502, Abcam, ｨｨｨｺ06ｨ 08ｨｨｨｰｨ ｨｪｨｨ07), 09 08ｨ ｨｪｨｨｨｨ 1 : ｨ ｨｺ0502ｨｰ06ｨｺ 06ｨｺ08ｨ 03ｨｨ09ｨ 05ｨｨ ｨｰ080607ｨｪ04ｨｬ ｨｺ08ｨ 09ｨｨｨｰ020502ｨｬ DAPI (Sigma, ) 09 ｨｺ06ｨｪ0202ｨｪｨｰ08ｨ 02ｨｨｨｨ 1 ｨｬｨｺ00/ｨｬ ｨ 08ｨ ｨｰ04 09 ｨｺ0502ｨｰｨｺｨ ｨｬｨｨ 04ｨ ｨｺ0506 ﾂｨ 05ｨｨ 09 ｨｬ0609ｨｨ0605 (Calbiochem, ) ｨｨ ｨｬｨ ｨｰ08ｨｨ09ｨ 05ｨｨ ｨｮ ｨｪｨｰｨｪ06ｨｬ ｨｬｨｨｨｺ080609ｨｺ (AxioCamMRc 5, Zeizz, ｨｬｨ ｨｪｨｨ07, 06ｨｺ , 06ｨ ) ｨｪｨｨ ｨ 00ｨｬ02ｨｪｨｰｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨ ｨｪｨ 08ｨｮ0302ｨｪｨｨ07 08ｨ ｨｨ ｨｹ040609ｨ 05ｨｨ ｨｬ02ｨｰ0601 TUNEL ｨｰｨｺｨｨ 00ｨｨｨｺ09ｨｨ080609ｨ 05ｨｨ ｨｨ 06ｨｺ08ｨ 03ｨｨ09ｨ 05ｨｨ ｨｬ06ｨｴｨｹ06 ｨｪｨ ｨ 0608ｨ 0802ｨ 0002ｨｪｨｰ0609 Dead End6ｾ4 Colorimetric TUNEL System (Promega, ). 0402ｨ ｨｺ02ｨｨ ｨｨ05ｨｨ ｨ 09ｨｪ ｨｰ06ｨｺ0605ｨｮ 00ｨｨ08ｨｬ ｨｨ ｨｨｨｰ ｨｪｨｨ ｨｨ ｨｨｨｬ ｨ 05ｨｹｨｪ ｨｬ02ｨｪｨｨ (TaqMan). 02ｨ ｨｴｨｮ (0708ｨｨｨｬ0208ｨｪ06 ｨｨ04 2 ｨｬ05ｨｪ ｨｺ0502ｨｰ06ｨｺ) ｨｨ ｨｬ06ｨｴｨｹ06 ｨｪｨ ｨ 0608ｨ Aurum Total RNA Minikit (BioRad, ) ｨ ｨ ｨｪｨｨ02ｨｬ ｨ 0404 I (BioRad, ) ｨ 01ｨ 02ｨｨｨｨ 0002ｨｪ06ｨｬｨｪ06ｨｦ ｨ 08ｨ ｨｰｨｪ06ｨｦ ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ ｨｨ ｨｹ040609ｨ 05ｨｨ 0708ｨ ｨｦｨｬ020804, ｨｰｨ ｨｴｨｨ ｨ 06ｨｦ 09ｨｬ0209ｨｹ 0605ｨｨ0006-dT ｨｨ 0905ｨｮ ﾂｨ ｨｦｨｪ ｨｺ09ｨ ｨｬ , ｨｨ 0108ｨｮ00ｨｨ ｨ 0002ｨｪｨｰ04 ｨｨ04 ｨｪｨ ｨ 0608ｨ iscriptcdna Synthesis Kit (Bio- Rad, ) ｨｰ0902ｨｰ09ｨｰ09ｨｨｨｨ ｨｰ06ｨｺ060506ｨｬ 00ｨｨ08ｨｬ ｨｨ ｨｨｨｰ ｨｪｨｨ ｨｨ ｨｹ040609ｨ 05ｨｨ 0708ｨ ｨｦｨｬ ｨｨ 0406ｨｪ0104, 08ｨ 0408ｨ ｨ 06ｨｰｨ ｨｪｨｪ0402 ｨｪｨ ｨｬｨｨ 08ｨ ｨｪ0202 (000605ｨｪｨｨ02ｨｺｨｨｨｦ ｨｨ 0108., 2012). 0402ｨ ｨｺ02ｨｨ06ｨｪｨｪｨ 07 09ｨｬ0209ｨｹ (50 ｨｬｨｺ05) ｨ 05ｨ 0601ｨｪ06ｨｺ08ｨ ｨｰｨｪ04ｨｦ ｨ ｨｮ Taq ｨｨｨｬ0208ｨ 0404 (ph 8.6), 1 ｨｬ ｨｨ01 Mg, Taq ｨｨｨｬ0208ｨ 0404 (05ｨｨ0502ｨｺ09, ｨｨ07), 250 ｨｬｨｺ00 ｨｺｨ dntp, 300 ｨｪ ｨｬ ｨｨ 06ｨ 08ｨ ｨｰｨｪ ｨ ｨｦｨｬ , 250 ｨｪ ｨｮ ｨｪｨｰｨｪ06 ｨｬ02 ﾂ02ｨｪｨｪ ｨｪ01ｨ (05ｨｨｨｪｨｰ0605, ｨｨ07) ｨｨ 2 ｨｬｨｺ05 ｨｺ ｨ ｨｺ02ｨｨ ｨｨ05ｨｨ ｨｪｨ 0102ｨｰ02ｨｺｨｰｨｨ08ｨｮ06ｨｴ02ｨｬ ｨ ｨｬ0705ｨｨ00ｨｨｨｺｨ ｨｰ CFX96 (BioRad, ). 0202ｨｪｨ ｨｰｨｮ08ｨ 02ｨｨ ｨｮ01ｨｪｨｨｨｰ020906ｨｦ ｨｪｨ ｨｺｨ ｨｬ ｨｨ ｨｨｨｺ ｨｮｨｴ0209ｨｰ ｨｨ 0708ｨｨ 95 紊 09 ｨｰ02 ﾂ02ｨｪｨｨ , ｨ 06ｨｰ03ｨｨ ｨ ｨｦｨｬ ｨｨ 0102ｨｰ02ｨｺｨｰｨｨ080609ｨ ｨｪｨｨ02 ｪ 0708ｨｨ 60 紊 09 ｨｰ02 ﾂ02ｨｪｨｨ02 1 ｨｬｨｨｨｪ ｨｪｨｨ ｨｨｨｺ ｨｨ05ｨｨ ｨｬ06ｨｴｨｹ ｨｰ080602ｨｪｨｪ06ｨｦ ｨｨｨ ｨ ｨｬｨｬ04 CFXManager. 04ｨ 09 ﾂ02ｨｰ 06ｨｰｨｪ0609ｨｨｨｰ0205ｨｹｨｪ0401 ｨｮ080609ｨｪ02ｨｦ ｨｨ ｨｨ05ｨｨ ｨｬ06ｨｴｨｹ ｨｬｨｮ0504R=2 cqref-cq, q ｨｨ cqref ｪ ｨｨｨｺ ｨｨ09ｨｺ06ｨｬ06ｨｦ ｨｨ ｨｪ09ｨｪ06ｨｦ ｨｺ ｨｪｨ GAPDH ｨｮ05ｨｹｨｰｨ ｨｰ ｨｰｨ ｨｪ ｨｨ ｨｰｨｪ060302ｨｪｨｨ07 ｨｮ080609ｨｪ02ｨｦ ｨｨ ｨｬ ｨｨ00ｨｨ ﾂ0209ｨｺｨｨ01 ｨｬ ｨｪｨ TP73 ｨｨ ｨｪ0608ｨｬｨｨ ﾂｨｪ ｨｪｨ GAPDH. 05ｨｰｨ ｨｰｨｨ09ｨｰｨｨ ﾂ0209ｨｺｨ 07 06ｨ 08ｨ ｨ 06ｨｰｨｺｨ ｨｮ05ｨｹｨｰｨ ｨｰ ｨ ｨｦ 05ｨｺ ｨｨｨｬ02ｨｪｨｰ ｨｨ05ｨｨ ｨｪ02 ｨｬ02ｨｪ ｨ ｨｰ

3 ｨ ｨｨ ｨｺ08ｨ ｨｰｨｪ04ｨｬｨｨ ｨｰ0608ｨ ｨｬｨｨ ﾂ02ｨｰ ｨｪｨｨ01 04ｨｪｨ ﾂ02ｨｪｨｨｨｦ ｨｨ 09ｨｰｨ ｨｪ01ｨ 08ｨｰｨｪ ｨｨｨ 06ｨｺ ｨｨ05ｨｨ 09 ｨｨ ｨｹ040609ｨ ｨｪｨｨ02ｨｬ 07ｨ ｨｺ02ｨｰｨ STATISTICA ｨｰ ｨｪ0609ｨｰｨｹ 08ｨ 0405ｨｨ ﾂｨｨｨｦ ｨｪｨｨ09ｨ 05ｨｨ 0706 t-ｨｺ08ｨｨｨｰ0208ｨｨ06 05ｨｰｨｹ060102ｨｪｨｰｨ. 04ｨ 0405ｨｨ ﾂｨｨ07 09 ﾂｨｨｨｰｨ 05ｨｨ ｨｰ ｨｪ04ｨｬｨｨ 0708ｨｨ 04 < ｨｹ040609ｨ ｨｪｨｪ ｨ ｨｺｨｰｨｨ ｨ %-ｨｪ06ｨｦ 05ｨｬｨ 08ｨｨ06ｨｪｨ 05ｨｹｨｪ06ｨｦ ｨｰ ﾂｨｹ02ｨｦ ｨｰｨｺ06ｨｦ (060605), L-0005ｨｮｨｰｨ ｨｬｨｨｨｪ06ｨｬ ｨｨ 0002ｨｪｨｰｨ ｨｬｨｨ02ｨｨｨｪ06ｨｬ (03ｨ ｨｪ06ｨｺ06, ｨｨ07); C 27 H 29 NO 11 (Sigma, ); 0002ｨｬｨ ｨｰ06ｨｺ09ｨｨ05ｨｨｨｪ 08ｨ 08ｨ ﾂ ﾂｨｨ (090608ｨｨ ｨ , ｨｨ07); ｨｨ00ｨｨ ﾂ0209ｨｺｨｨ02 ｨ ｨｪｨｰｨｨｨｰ0205ｨ ab52293, ab4052, ab32539 ｨｨ ab58610 (Abcam, ｨｨｨｺ06ｨ 08ｨｨｨｰｨ ｨｪｨｨ07); ｨｺ0604ｨｹｨｨ ｨ ｨｪｨｰｨｨｨｺ080605ｨｨ ﾂｨｹｨｨ ｨｨｨｺ0506ｨｪｨ 05ｨｹｨｪ ｨｰ ｨ ｨｪｨｰｨｨｨｰ0205ｨ, ｨｺ06ｨｪｨｲ0600ｨｨ080609ｨ ｨｪｨｪ ｨｮ ｨｬ06ｨｬ , ｪ ab98502; DAPI (Sigma, ); ｨｬ0609ｨｨ0605 (Calbiochem, ); ｨｪｨ ｨ ｨ 0002ｨｪｨｰ0609 DeadEnd TM ColorimetricTUNEL- System (Promega, ); ｨｪｨ ｨ 0608 AurumTotalRNAMinikit ｨ 0406ｨｦ I (BioRad, ); ｨｪｨ ｨ 0608 iscriptcdnasynthesis- Kit (BioRad, ); ｨ ｨｮ Taq ｨｨｨｬ0208ｨ 0404, ｨｨ01 ｨｬｨ 00ｨｪｨｨ07, Taq ｨｨｨｬ0208ｨ 04ｨ ｨｨ dntp (05ｨｨ0502ｨｺ09, ｨｨ07); 0708ｨ ｨｦｨｬ ｨｨ 0005ｨｮ ｨｪｨｰｨｪ06 ｨｬ02 ﾂ02ｨｪｨｪ ｨｪ0104 (05ｨｨｨｪｨｰ0605, ｨｨ07) ｨｮ05ｨｹｨｰｨ ｨｰ04 00ｨｨｨｰ06ｨｰ06ｨｺ09ｨｨ ﾂ0209ｨｺ ｨｦ09ｨｰ09ｨｨ ｨｪｨ 00ｨｨｨ ｨ 05 ｨ 09 ｨｰ 04 ﾂ ｨｺ ｨ ﾂ02ｨｰ ｨｺ0502ｨｰ06ｨｺ ﾁ ｨｪｨ 02ｨｨｨｰ ｨｨ ﾂ0209ｨｺｨｨ ｨ 08ｨ ｨｰｨ ｨｺｨ 04ｨ 05, ﾂｨｰ06 ﾂ ﾂ ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨ07 ﾂｨｨ ｨｺ0502ｨｰ06ｨｺ 09 ｨｺ06ｨｪｨｰ080605ｨｹｨｪ06ｨｦ (ｨｪ02ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨｨ ｨｪ02 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ06ｨｦ ) ｨｺｨｮ05ｨｹｨｰｨｮ ｨｰｨ ｨｪｨ ｨｪｨｨ07 ｨｬｨｨｨｺ080609ｨｺ0607ｨ. 00 ｨｬ06ｨｬ02ｨｪｨｰ 09ｨｪ020902ｨｪｨｨ ｨｺｨｮ05ｨｹｨｰｨｮ08ｨｮ ( ﾂ ﾂ ｨｪｨ ﾂｨ 05ｨ ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨ07) ﾂｨｨ ｨｺ0502ｨｰ06ｨｺ ｨｮ090205ｨｨ ﾂｨｨ050609ｨｹ ｨｨ ｨｰｨ 09ｨｨ ｨｪｨ ｨｪｨｨ07. 05ｨ ｨｮ06ｨｴｨｨ02 24 ﾂ (72 ﾂ ｨ 01ｨｺｨｨ) 09 ｨｺ06ｨｪｨｰ080605ｨｹｨｪ06ｨｦ (ｨｪ02 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ06ｨｦ ) ｨｺｨｮ05ｨｹｨｰｨｮ0802 ﾂｨｨ ｨｺ0502ｨｰ06ｨｺ ｨｮ090205ｨｨ ﾂｨｨ050609ｨｹ ｨｨ ｨｰｨｨ ｨｪｨ ｨｪｨｨ ｨｰ ｨｬ07 09 ｨｺｨｮ05ｨｹｨｰｨｮ0802, 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ06ｨｦ ｨｰ02 ﾂ02ｨｪｨｨ02 24 ﾂ, 06ｨｰｨｬ02ｨｰｨｨ05ｨｨ ｨｺ ｨｪｨｨ0302ｨｪｨｨ02 ﾂｨｨ0905ｨ 00ｨｨｨ 0806ｨ 05ｨ 09ｨｰ ｨｪｨ ｨｪｨｨ07 (P <0.05) (08ｨｨ09. 1; 2, ｨ ). 06ｨｰ ｨｪｨ ﾂｨ 02ｨｰ, ﾂｨｰ06 06ｨ 08ｨ ｨ 06ｨｰｨｺｨ ﾁ ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺ06ｨｬ 09 ｨｰ02 ﾂ02ｨｪｨｨ02 24 ﾂ 09 ｨｺ06ｨｪ0202ｨｪｨｰ08ｨ 02ｨｨｨｨ 5 ｨｬｨｺ00/ｨｬ ｨ 02ｨｰ 00ｨｨｨ 0205ｨｹ 06ｨｺ % ｨｺ0502ｨｰ06ｨｺ, ｨｨ 0909ｨｨ0102ｨｰ0205ｨｹ09ｨｰ09ｨｮ02ｨｰ ｨｺ06ｨｦ ｨｰ06ｨｺ09ｨｨ ﾂｨｪ0609ｨｰｨｨ ｨｰｨｪ060302ｨｪｨｨｨｨ 00ｨｨｨ 0806ｨ 05ｨ 09ｨｰ0609 ﾂ ｨｺｨ ｨｮ ﾂ02ｨｪｨｪ ｨ ｨｪｨｪ ｨｪ ｨｮ05ｨｹｨｰｨ ｨｰｨ ｨｬｨｨ ｨ 09ｨｰ , ｨｺ06ｨｰ ｨｺｨ 04ｨ 05ｨｨ 02ｨｨｨｰ06ｨｰ06ｨｺ09ｨｨ ﾂ0209ｨｺ ｨｦ09ｨｰ09ｨｨ ｨｪｨ 01ｨｨ ｨｪ02ｨｨ080609ｨ ｨｪｨｪ ｨｨｨ 0806ｨ 05ｨ 09ｨｰ04 ﾂ ｨｺｨ (080603ｨｮ01ｨ ｨ ｨｨ 0108., 2012). 04ｨｨ ｨｨ07ｨｪｨｨ ｨｪｨ 00ｨｨｨ 0205ｨｹ ｨｺ0502ｨｰ06ｨｺ 09 ｨｪ02ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ06ｨｦ (000000ｨC) ｨｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ06ｨｦ ( ) ｨｺｨｮ05ｨｹｨｰｨｮ08ｨ ﾁ ｨｰｨｺｨｨ ﾁ ﾂ ﾂ ｨ 04ｨ 08ｨ 03ｨ 05ｨｨ ﾁ ﾂ ｨ 08ｨ 0302ｨｪｨｨ07 09ｨｪ0609ｨｨ05ｨｨ ｨｨ ｨｨｨｪｨｺｨｮｨ ｨｨ080609ｨ 05ｨｨ ｨｮ06ｨｴｨｨ02 24 ﾂ. 0202ｨｦ09ｨｰ09ｨｨ ｨｪｨ 00ｨｨｨ 0806ｨ 05ｨ 09ｨｰ04 ﾂ ｨｺｨ, ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ｨｪｨｺｨｨ 0102ｨｦ09ｨｰ09ｨｨ ｨｺ0502ｨｰｨｺｨｨ ﾁ ﾂ ﾂ ｨｪｨ ﾂｨ 05ｨ ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨ07 04ｨ 08ｨ 03ｨ 05ｨｨ 09ｨｨ08ｨｮ0906ｨｬ ｨｨ ｨｨ09ｨ 05ｨｨ 09 ｨｰ02 ﾂ02ｨｪｨｨ ｨｮ06ｨｴｨｨ01 24 ｨｨ 48 ﾂ (48 ｨｨ 72 ﾂ ｨ 01ｨｺｨｨ ｨｺ0502ｨｰ06ｨｺ ｨｰ0902ｨｰ09ｨｰ0902ｨｪｨｪ06). ﾁｨｨ ｨｺ0502ｨｰ06ｨｺ ｨ 08ｨ ｨｰ02 09ｨｪｨｨ04ｨｨ050609ｨｹ ｨ 09ｨｪ02ｨｪｨｨ06 09 ｨｺ06ｨｪｨｰ080605ｨｹｨｪ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0806ｨｦ ｨｨ ｨｰｨ 09ｨｨ ｨｨ ｨｪｨ ｨｪｨｨ07 (09 ｨｺ06ｨｪｨｰ080605ｨｹｨｪ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0802 ｪ ｨｨ ｨｰ0902ｨｰ09ｨｰ0902ｨｪｨｪ06) (08ｨｨ09. 1). 01ｨｨｨ 0205ｨｹ ｨｺ0502ｨｰ06ｨｺ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ ｨｰｨ 09ｨｨ05ｨ 40 % ﾂ ﾂ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｨ07 ｨｨ 59 % ﾂ ﾂ ｨ 09ｨｪ02ｨｪｨｨ06 09 ｨｪ0204ｨ 08ｨ 0302ｨｪｨｪ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0806ｨｦ. 0308ｨｨ 09ｨｪ020902ｨｪｨｨｨｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪｨｮ06 ｨｺｨｮ05ｨｹｨｰｨｮ08ｨｮ ﾁ ﾂｨｨ ｨｺ0502ｨｰ06ｨｺ ﾂ ﾂ ｨｰｨ 09ｨｨ ｨｪｨ ｨｪｨｨ07, ﾂｨｰ ｨ ｨｺｨｰｨｨ ﾂ0209ｨｺｨｨ ｨｪ02 06ｨｰ05ｨｨ ﾂｨ ｨｹ 06ｨｰ ｨｺ0605ｨｨ ﾂ0209ｨｰ09ｨ ｨｺ0502ｨｰ06ｨｺ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0802 ｨ ｨ 08ｨ ｨ 06ｨｰｨｺｨｨ ｨｨ ｨ ｨ 04ｨ ｨ 0605ｨｹ0302, ﾂ02ｨｬ 09 ｨｪ0204ｨ 08ｨ 0302ｨｪｨｪ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ ﾁ, ｪ ｨｺ0502ｨｰ06ｨｺ ｨｪｨ ｨｪｨｨ07 (04 < 0.05). 06ｨ ｨｺｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ, ﾁ, ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ , ｨｬ06ｨｪ09ｨｰ08ｨｨ080609ｨ 05ｨｨ ｨ 0605ｨｹ03ｨｮ06 ｨｮ09ｨｰ06ｨｦ ﾂｨｨ090609ｨｰｨｹ ｨｺ 02ｨｨｨｰ06ｨｰ06ｨｺ09ｨｨ ﾂ0209ｨｺ06ｨｬｨｮ 0102ｨｦ09ｨｰ09ｨｨ , ﾂ02ｨｬ ｨｪ0204ｨ 08ｨ 0302ｨｪｨｪ0402 ｨｺ0502ｨｰｨｺｨｨ ｨｪ06 04ｨ ｨｺ0506- ﾂｨｨｨｰｨｹ, ﾂｨｰ06 08ｨ 0409ｨｨｨｰｨｨ ｨｨｨｪ0002ｨｺ02ｨｨｨｨ 09 00ｨｨｨ 0806ｨ 05ｨ 09ｨｰｨ ｨ ｨ ｨｰ ｨｰ06ｨｺ09ｨｨ ﾂ0209ｨｺ ｨｦ09ｨｰ09ｨｨ ｨｪｨ ｨｮ050702ｨｨ06 ｨｺ0502ｨｰ06ｨｺ ﾁ. 0005ｨｨ07ｨｪｨｨ ｨｪｨ ｨｨ000208ｨ 02ｨｨ06 01ｨｨ070506ｨｨ01ｨｪ ｨｨｨ 0806ｨ 05ｨ 09ｨｰ0609 ﾂ ｨｺｨ ｨｨ04ｨｮ ﾂ02ｨｪｨｨ ｨｨ07ｨｪｨｨ ｨｪｨ ｨｨ000208ｨ 02ｨｨ06 ｨｨ ｨｹ040609ｨ 05ｨｨ ｨｬ ｨｨ ﾂ0209ｨｺｨｨｨｦ ｨｺ08ｨｨｨｰ0208ｨｨｨｦ ｪ ｨｨ0102ｨｪｨｰｨｨ00ｨｨｨｺｨ 02ｨｨ06 ｨｺ0502ｨｰ06ｨｺ, ｨｪｨ ｨｴｨｨ ｨｬｨｨｨｰ060402, ｨｪｨ 02ｨｨｨｰ ｨｨ ﾂ0209ｨｺ06ｨｬ ｨ 08ｨ ｨｰ02 ｨｨ ｨｪｨｺｨｮ ｨｨ01 ｨｺ0605ｨｨ ﾂ0209ｨｰ09ｨ ﾂ02ｨｰ ｨｬｨｨｨｰ06ｨｰｨｨ ﾂ0209ｨｺｨｨ01 00ｨｨ00ｨｮ ｨｺ08ｨ 09ｨｺｨｨ ｨ 06ｨｴｨｨｨｬ ｨｺ08ｨ 09ｨｨｨｰ020502ｨｬ DAPI 0706ｨｺｨ 04ｨ 05, ﾂｨｰ06 09 ｨｬ06ｨｬ02ｨｪｨｰ 09ｨｪ020902ｨｪｨｨ07 ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺｨ ﾁ ｨｪｨ ｨｨ05ｨｨ09ｨｹ ｨｰ0607ｨｪｨｨｨｨ ｨ ｨｺｨｰｨｨ09ｨｪ ｨｪｨｨ07 ｪ ｨｺ0502ｨｰ06ｨｺ 09 00ｨ ｨｺ0502ｨｰ06 ﾂｨｪ ｨｨｨｺ05ｨ ｨｰｨ 09ｨｨ05ｨ ﾁ ﾂ 09 ｨｺ06ｨｪｨｰ080605ｨｹｨｪ06ｨｦ (ｨｪ02 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ06ｨｦ ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺ06ｨｬ) ｨｺｨｮ05ｨｹｨｰｨｮ ｨｬｨｨｨｰ ｨｮｨｬ02ｨｪｨｹ03ｨｨ05ｨ 09ｨｹ 0706 ﾂｨｰｨｨ ｨ 04ｨ, ｨｰｨ 09ｨｨ (P < 0.05). 05ｨｪｨｨ0302ｨｪｨｨ ｨｨ ｨｬｨｨｨｰ06ｨｰｨｨ ﾂ0209ｨｺｨｨ01 ｨｺ0502ｨｰ06ｨｺ ｨｬ0603ｨｪ06 06ｨ ｨｲ0709ｨｪｨｨｨｰｨｹ ｨｰ02ｨｬ, ﾂｨｰ06 ﾂｨ 09ｨｰｨｹ ｨｺ0502ｨｰ06ｨｺ ｨ ｨ 0404 ｨｬｨｨｨｰ0604ｨ ｨｨ 08ｨ ｨｨ05ｨ 09ｨｹ, ｨ 0609ｨｰｨ 0903ｨｨ ｨｺ0502ｨｰｨｺｨｨ 04ｨ ｨｬ020105ｨｨ05ｨｨ ｨｬｨｨｨｰ06ｨｰｨｨ ﾂ0209ｨｺ ｨｪｨｨ02 ｨｨ 0609ｨｰｨ ｨｪ0609ｨｨ05ｨｨ09ｨｹ 09 00ｨ 04ｨ 01, ｨｰ09ｨｮ06ｨｴｨｨ01 ｨｬｨｨｨｰ0604ｨｮ, ｨｨ ｨｰｨｨ0302ｨｪｨｨ02ｨｬ ｨ 0605ｨｹ0302ｨｦ ｨｰｨｪ0609ｨｰｨｨ ｨｬ06ｨｪ , ｨｰｨ ｨｺ ｨｺｨ ｨｺ ﾁ ｪ ｨｪ020607ｨｮ ｨｺ0502ｨｰｨｺｨｨ, 06ｨ 05ｨ 01ｨ 06ｨｴｨｨ ｨｦ09ｨｰ0906ｨｬ ｨｺ06ｨｪｨｰｨ ｨｺｨｰｨｪ ｨｰ0608ｨｬ060302ｨｪｨｨ ｨｪｨｨ ｨｺｨｮ05ｨｹｨｰｨｮ0802, 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ06ｨｦ , ｨｬｨｨｨｰ06ｨｰｨｨ ﾂ0209ｨｺｨｨ01 00ｨｨ00ｨｮ ｨ ｨｪ0705ｨ 09ｨｹ ｨｪｨ ｨｺ06ｨｬ ｨｮ080609ｨｪ02 ( ) ｨｮ ﾂ02ｨｪｨｪ ｨ ｨｪｨｪ ｨｰ ｨｨｨｰｨｹ, ﾂｨｰ ｨ 0506ｨｺｨｨ08ｨｮ02ｨｰ ｨｺ0502ｨｰｨｺｨｨ 09 ｨｬｨｨｨｰ060402, ｨｨ 05ｨｰ06, ｨｨ01ｨｨｨｬ06ｨｬｨｮ, ｨｰ ｨｪｨｨｨｬ ｨｨ ｨｪｨｨｨｦ ｨｨｨｰ0609ｨｰｨ ｨｰｨｨ ﾂ0209ｨｺ ｨｦ09ｨｰ09ｨｨ ｨ ｨ 06ｨｰ, ｨｪ02ｨｪｨｪ0401 ｨｬ02ｨｰ060106ｨｬ ｨｰ06 ﾂｨｪ06ｨｦ 02ｨｨｨｰ06ｨｬ02ｨｰ08ｨｨｨｨ , 06ｨｰｨｬ02 ﾂｨ 05ｨｨ, ﾂｨｰ ｨ 0506ｨｺｨｨ08ｨｮ02ｨｰ ｨｺ0502ｨｰｨｺｨｨ 09 00ｨ 04ｨ 01 G 2 /M ｨｺ0502ｨｰ06 ﾂｨｪ ｨｨｨｺ05ｨ (Cruet-Hennequart et al., 2012; Fan et al., 2014). 06ｨ ｨｺ ｨｺｨ ｨｺ ｨｬ02ｨｰ0601 ｨｪ ｨｰ 08ｨ 0405ｨｨ ﾂｨｨｨｰｨｹ 00ｨ 0404 ｨｺ0502ｨｰ06 ﾂｨｪ ｨｨｨｺ05ｨ, ｨｰ0902ｨｰ09ｨｰ09ｨｮ06ｨｴｨｨ02 ｨｮ ｨｪｨｪ06ｨｬｨｮ ｨ ｨｪｨｨ (4N), ｨ 09ｨｰ ｨｺｨ 04ｨ 05ｨｨ ｨｪｨｨ02 06 ｨｰ06ｨｬ, ﾂｨｰ06 ﾂｨ 09ｨｰｨｹ 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ ｨｺ0502ｨｰ06ｨｺ ｨｬ060302ｨｰ 0609ｨｰｨ ｨｪｨ 0905ｨｨ09ｨ ｨｰｨｹ0907 ｨｪ ｨ 04ｨ 01 G 2 /M, ｨ ｨｨｨｰｨｹ 09 00ｨ 04ｨｮ G 1 09 ｨｮ ｨｪｨｪ04ｨｬ ｨ ｨｪｨｨ02ｨｬ ｪ ｨｰｨ ｨｺ ｨｪｨ ｨ 02ｨｬｨｮ06 00ｨ 04ｨｮ 4NG 1, ｨｪ ｨｦ0107 ｨｬｨｨｨｰ06ｨｰｨｨ ﾂ0209ｨｺ ｨｪｨｨ07 (Bielak-Zmijewska et al., 2014) ｨｮ ﾂ02ｨｪｨｪ0402 ｨｪｨ ｨｬｨｨ 01ｨ ｨｪｨｪ ｨｨ0102ｨｰ0205ｨｹ09ｨｰ09ｨｮ06ｨｰ 06 ｨｰ06ｨｬ, ﾂｨｰ06 09 ｨｮ ｨｨ0701 ｨｪｨ 03ｨｨ ｨｰ0609 ｨ 0605ｨｹ03ｨ 07 ﾂｨ 09ｨｰｨｹ ｨｺ0502ｨｰ06ｨｺ ﾁ ｨｦ09ｨｰ09ｨｨ02ｨｬ ｨｰｨ ｨｪｨ 0905ｨｨ09ｨ 02ｨｰ ｨｬｨｨｨｰ ｨｨ ｨｪ ｨｨｨｰ 09 09ｨｰｨ 01ｨｨ06 G 1. 08ｨｪｨ 05ｨｨ04 ｨｬｨｨｨｰ06ｨｰｨｨ ﾂ0209ｨｺｨｨ01 00ｨｨ00ｨｮ08 09 ｨｺ0502ｨｰｨｺｨ ﾁ, ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ , 0706ｨｺｨ 04ｨ 05, ﾂｨｰ ｨｺ0502ｨｰ06ｨｺ, ｨｪｨ ｨｴｨｨ ｨｬｨｨｨｰ060402, ｨｰｨ 09ｨｨ05ｨ ｨｦ09ｨｰ09ｨｨ ｨｪｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0402 ｨｺ0502ｨｰｨｺｨｨ 05ｨｰ06ｨｰ 0706ｨｺｨ 04ｨ -

4 0005ｨｨ07ｨｪｨｨ02 ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺｨ 0106ｨｺ090608ｨｮｨ ｨｨ02ｨｨｨｪｨ ｨｪｨ 00ｨｨｨ 0806ｨ 05ｨ 09ｨｰ04 ﾂ ｨｺｨ, ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ｨｨｨｰ06ｨｬ0200ｨ ｨｨ08ｨｮ0906ｨｬ ｨｨ ｨｦ09ｨｰ09ｨｨ (ｨ, 09) ｨｨ (01, 03) ｨｪｨ ﾁ in vitro. ｨ ｪ 02ｨｨｨｰ06ｨｰ06ｨｺ09ｨｨ ﾂ0209ｨｺ ｨｦ09ｨｰ09ｨｨ ｨｪｨ ﾁ ﾂ ﾂ ｨｪ020902ｨｪｨｨ07 ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺｨ 09 ｨｺｨｮ05ｨｹｨｰｨｮ08ｨｮ; ｨ ｪ ｨｺ06ｨｪｨｰ080605ｨｹ, 06ｨｺ08ｨ 03ｨｨ09ｨ ｨｪｨｨ ｨｬｨ ｨｰ06ｨｺ09ｨｨ05ｨｨｨｪ06ｨｬ 08ｨ 08ｨ ﾂ ﾂｨｨ; 09 ｪ 09ｨｨ04ｨｮｨ 05ｨｨ04ｨ 02ｨｨ ｨ 00ｨｬ02ｨｪｨｰｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨｬ02ｨｰ060106ｨｬ TUNEL 09 ｨｺ0502ｨｰｨｺｨ 01 ﾂ ﾂ ｨ 08ｨ ｨ 06ｨｰｨｺｨｨ ; 00, ｨ ｨｰｨ ｨｺ , 04 ｪ ｨｰ ｨｺ0502ｨｰｨｺｨｨ, 06ｨｺ08ｨ 0302ｨｪｨｪ0402 DAPI; 01 ｪ 0102ｨｰ02ｨｺ02ｨｨ ｨｬ06ｨｴｨｹ06 ｨ ｨｪｨｰｨｨｨｰ0205 ｨ ｨｺｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨｺｨ 0907ｨ ｨｺ0502ｨｰｨｺｨ 01 ﾂ ﾂ ｨ 08ｨ 0302ｨｪｨｨ ; 03 ｪ 0102ｨｰ02ｨｺ02ｨｨ07 ｨｬｨ 08ｨｺ0208ｨ ｨ ｨｮｨｰ0600ｨ 00ｨｨｨｨ LCP3A/B ｨｬ06ｨｴｨｹ06 ｨ ｨｪｨｰｨｨｨｰ ｨｺ0502ｨｰｨｺｨ 01 ﾂ ﾂ ｨ 08ｨ 0302ｨｪｨｨ ｨｰ0205ｨｹ ｨｪ02 ｨｨ04ｨｬ02ｨｪｨｨ ｪ 6 ) 2. 06ｨｰ06 04ｨｪｨ ﾂ02ｨｪｨｨ ｨ 04ｨ ｨｪｨｨ0302, ﾂ02ｨｬ 05ｨｰ06ｨｰ ｨｺｨ 04ｨ ｨｰ0205ｨｹ ｨｮ050702ｨｨｨｨ ｨｪ02ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ﾁ, 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ (18 ) 2 ) ｨｮ ﾂ02ｨｪｨｪ ｨ ｨｪｨｪ ｨｰ 04ｨ ｨｺ0506 ﾂｨｨｨｰｨｹ, ﾂｨｰ ｨｨ ｨｦ09ｨｰ09ｨｨｨｨ ｨｪｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ｨｨｨ 0806ｨ 05ｨ 09ｨｰ ｨｰ0908ｨ ｨｴｨ 02ｨｰ0907 ｨ 0506ｨｺ ｨｬｨｨｨｰ0604ｨ 09 06ｨｰ05ｨｨ ﾂｨｨ02 06ｨｰ ｨｮ050702ｨｨｨｨ ｨｪ0204ｨ 08ｨ 0302ｨｪｨｪ ﾁ, 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ ｨｰｨｨｨｬ ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺ06ｨｬ ｨｪ ｨｨ ｨｪｨｨｨｬ ｨｨ04 ｨｬ0201ｨ ｨｪｨｨ04ｨｬ ｨｰｨｨ ｨｮ ｨｦ09ｨｰ09ｨｨ ｨｰ ｨ ｨｪ0609ｨｰｨｹ ｨ ｨｰｨｹ 0601ｨｨｨｪ06 ﾂｨｪ0402 ｨｨ ｨｦｨｪ ｨ (Burden, Osheroff, 1998; Tsang et al., 2003) ｨｰｨ ｨｨｨｪｨｰ ｨｪｨｨｨｰｨｹ 0905ｨｨ07ｨｪｨｨ ｨｪｨ ｨｰｨｪ0609ｨｰｨｹ ﾁ ｨｰ ｨｺ06ｨｪｨｰ080605ｨｹｨｪ0402 (ｨｪ0206ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ0402) ｨｨ ｨｰｨｪ0402 (06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ ) ｨｺ0502ｨｰｨｺｨｨ ｨｨ04ｨｮ ﾂｨ 05ｨｨ ｨｬ06ｨｴｨｹ06

5 ｨ ｨｨ ｨｨ ｨｪｨｨ02 ｨｺｨ 0907ｨ 04 9, 8 ｨｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0401 ｨｺ0502ｨｰｨｺｨ 01 ｨ ｨ 08ｨ ｨ 06ｨｰｨｺｨｨ ｨｨ ﾂ ﾂ ｨｦ09ｨｰ09ｨｨ07 ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺｨ. ｨｬ02ｨｰ0601ｨ TUNEL, ｨｴ ｨ ｨｪｨ 08ｨｮ03ｨｨｨｰｨｹ 08ｨ ｨ 08ｨｨ09. 2, ｨｰｨ ｨｪ ｨｮ05ｨｹｨｰｨ ｨｰ04 06ｨｺ08ｨ 03ｨｨ09ｨ ｨｪｨｨ ﾁ ｨｬ02ｨｰ060106ｨｬ TUNEL ﾂ02ｨｰ TUNEL ｨｨｨｰ0205ｨｹｨｪ0401 ｨｺ0502ｨｰ06ｨｺ ( ﾁ ｨ 00ｨｬ02ｨｪｨｰｨｨ080609ｨ ｨｪｨｪ06ｨｦ ) 0706ｨｺｨ 04ｨ 05, ﾂｨｰ06 09 ｨｺ06ｨｪｨｰ080605ｨｹｨｪ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ ｨｺ0502ｨｰ06ｨｺ ｨｪｨｪ06ｨｦ ｨ 0405ｨ ｨｪ0204ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪ06ｨｦ ｪ % ｨｨ07ｨｪｨｨ ｨｺ0502ｨｰ06ｨｺ ｨ 00ｨｬ02ｨｪｨｰｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨｺ ｨ, ｨｰｨｨ00ｨｪｨｮ %, ﾂｨｰ ｨ ｨ ｨｰ06ｨｰ 0706ｨｺｨ 04ｨ ｨｰ0205ｨｹ 09 ｨｺ06ｨｪｨｰ080605ｨｹｨｪ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0802 (04 <0.05) ｨｪ06 04ｨ ｨｺ0506 ﾂｨｨｨｰｨｹ, ﾂｨｰ ｨｪｨｨｨｬ ｨｨ04 ｨｬ0201ｨ ｨｪｨｨ04ｨｬ0609, 0708ｨｨ ｨｴｨｨ01 ｨｺ 04ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪ06ｨｬｨｮ 02ｨｨｨｰ06ｨｰ06ｨｺ09ｨｨ ﾂ0209ｨｺ06ｨｬｨｮ 0102ｨｦ09ｨｰ09ｨｨ ｨｪｨ 00ｨｨｨ 0806ｨ 05ｨ 09ｨｰ04, ｨｰ0907 ｨｨｨｪ01ｨｮｨｺ02ｨｨ07 08ｨ ｨｬ060502ｨｺｨｮ ｨ ｨｪ ｨｦ09ｨｰ09ｨｨ02 06ｨｰｨｬ02 ﾂ02ｨｪ ｨｨ ｨｦ09ｨｰ09ｨｨｨｨ ｨｨ ｨｪｨ 0108ｨｮ00ｨｨ02 ｨｺ0502ｨｰｨｺｨｨ ｪ 09ｨｺ020502ｨｰｨｪ0401 ｨｬ (Yu et al., 2014), 0702 ﾂ02ｨｪｨｨ (Wang et al., 2014) ｨｨ ｨ (Lian et al., 2013) ｨｦ09ｨｰ09ｨｨ07 ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺｨ ｨｪｨ ｨｺ0502ｨｰｨｺｨｨ ﾁ, 04ｨ 08ｨ 0302ｨｪｨｪ , ｨｺ0502ｨｰ06ｨｺ ｨｪｨｪ04ｨｬｨｨ 08ｨ ｨ ｨｬｨｨ ｨｮ090205ｨｨ ﾂｨｨ05ｨ 09ｨｹ ｨ 09ｨｪ02ｨｪｨｨ06 09 ｨｺ0502ｨｰｨｺｨ ｨｬｨｨ, ｨｪ02 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ04ｨｬｨｨ , ｨｨ ｨｰｨ 09ｨｨ05ｨ ｨｰｨｨ % (P < 0.05). 0201ｨｪｨ ｨｺ06 09 ｨｪ02ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨｮ050702ｨｨｨｨ ｨｺ0502ｨｰ06ｨｺ ｨｪｨｪ06ｨｦ ｨｦ09ｨｰ09ｨｨ ｨｮ090205ｨｨ ﾂｨｨ05ｨ 09ｨｹ ｨ ﾂ02ｨｬ ｨ 04, ｨｰ060001ｨ ｨｺｨ ｨｺ 09 ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨｮ050702ｨｨｨｨ ｪ ｨ 04ｨ ｨｮ ﾂ02ｨｪｨｪ ｨ ｨｪｨｪ ｨｺｨ ｨ 06ｨｰ, ﾂｨｰ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0401 ｨｺ0502ｨｰｨｺｨ 01 ｨｨｨｪ01ｨｮ02ｨｨ08ｨｮ06ｨｰ0907 ｨｬ0201ｨ ｨｪｨｨ04ｨｬ04, ｨｰ09ｨｰ09ｨｮ06ｨｴｨｨ02 08ｨ 0409ｨｨｨｰｨｨ , 0708ｨｨ ｨｴｨｨ01 ｨｺ 08ｨ ｨ ｨｬ ｨｨ ｨｦ09ｨｰ09ｨｨｨｨ ｨｪｨｨ02 ｨｬｨ 08ｨｺ ｨ ｨｰ0604ｨ ｨｨ ｨ ｨｮｨｰ0600ｨ 00 ｨｨ ｨｨ. 0002ｨｰ060106ｨｬ ｨｨｨｬｨｬｨｮｨｪ060005ｨｮ ｨｪ02ｨｨｨｨ in situ ｨｪ02 ｨｮ01ｨ ｨｹ ｨｨｨｰｨｹ 09 ｨｺ06ｨｪｨｰ080605ｨｹｨｪ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0802 ｨｺ0502ｨｰ06ｨｺ, ｨ ｨｴｨｨ01 ｨ ｨｺｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｪ ｨｬ04 ｨｺｨ 0907ｨ 04 3, 8 ｨｨ 9,ｨｺ06ｨｰ ｨｰ0907 ｨｬｨ 08ｨｺ0208ｨ ｨｬｨｨ ｨ ｨｬｨｬｨｨ08ｨｮ02ｨｬ06ｨｦ 00ｨｨｨ 0205ｨｨ ｨｺ0502ｨｰ06ｨｺ ｨ 08ｨ ｨ 06ｨｰｨｺｨｨ ｨｰ02 ﾂ02ｨｪｨｨ02 8 ﾂ 09 ｨｪ0204ｨ 08ｨ 0302ｨｪｨｪ0401 ｨｺ0502ｨｰｨｺｨ 01 ｨｺｨ 0907ｨ 04ｨｮ ｨｨ05ｨｨ % ｨｺ0502ｨｰ06ｨｺ, ｨ ｨｺｨ 0907ｨ 04 8ｨｨ906ｨ ｨｪｨ 08ｨｮ0302ｨｪ06 ｨｪ02 ｨ ﾁ ﾂ ｨ 08ｨ ｨ 06ｨｰｨｺｨｨ ｨｺ0502ｨｰ06ｨｺ, ｨ ｨｴｨｨ01 ｨ ｨｺｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｪｨｮ06 ｨｺｨ 0907ｨ 04ｨｮ 3, ｨｮｨｬ02ｨｪｨｹ03ｨｨ05ｨ 09ｨｹ %. 00 ｨｰ ｨｬ07 ｨｺ0502ｨｰ06ｨｺ, ｨ ｨｴｨｨ01 ｨｺｨ 0907ｨ ｨｨ 9, ｨｮ050702ｨｨｨｨ 06ｨ ｨｪｨ 08ｨｮ0302ｨｪ06 ｨｪ02 ｨ ｨｰ09ｨｮｨｰ09ｨｰ09ｨｨ02 ｨｺｨ 0907ｨ 04 8 ｨｨ 9, ｨｮ ﾂｨ 09ｨｰ09ｨｮ06ｨｴｨｨ01 09 ｨｨｨｪｨｨ02ｨｨｨ 02ｨｨｨｨ ｨ ｨｰ0604ｨ, ｨｬ0603ｨｪ06 06ｨ ｨｲ0709ｨｪｨｨｨｰｨｹ ｨｪ ｨｰｨ ｨｰ06 ﾂｨｪ06ｨｦ ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0609ｨｰｨｹ06 ｨｬ02ｨｰ0601ｨ 0102ｨｰ02ｨｺ02ｨｨｨｨ ｨｨ09ｨｺ0506 ﾂ02ｨｪ06 ｨｰｨ ｨｺ0302, ﾂｨｰ06 ｨｺｨ 0907ｨ 04ｨ 8 ｨｪ02 ｨ ｨｺｨｰｨｨ09ｨｨ08ｨｮ02ｨｰ ｨｺ0502ｨｰｨｺｨ ﾁ, ｨ ｨｪ06 ｨｰ06ｨｬｨｮ ｨｺｨ ｨｺ 05ｨｰ06 06ｨｰｨｬ02- ﾂ02ｨｪ ｨｨ ｨｨ04ｨｮ ﾂ02ｨｪｨｨｨｨ 09ｨｰ0806ｨｬｨ 05ｨｹｨｪ0401 ｨｺ0502ｨｰ06ｨｺ (S-type) 09 ｨｪ02ｨｦ0806ｨ 05ｨ 09ｨｰ06ｨｬ02 (Bian et al., 2004) ｨｪｨｨ02 ｨ ｨｺｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨｬ ｨｺｨｰ0608ｨｪ06ｨｦ ｨｺｨ 0907ｨ (executioner) ｨｨｨｪ02 ｨｺ0502ｨｰ06ｨｺ ﾁ ﾂ ﾂ ｨ 08ｨ ｨ 06ｨｰｨｺｨｨ ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺ06ｨｬ ｨｮｨｺｨ ｨ 02ｨｰ ｨｪｨ ｨｮ ﾂｨ 09ｨｰｨｨ02 ｨｺｨ 0907ｨ ｨ 09ｨｨ09ｨｨｨｬ ｨｬｨ ｨｦ 00ｨｨｨ 0205ｨｨ 00ｨｨｨ 0806ｨ 05ｨ 09ｨｰ0609 ﾂ ｨｺｨ ｨｺ 24 ﾂ ｨｦ09ｨｰ09ｨｨ ｨｪｨｨ0302ｨｪｨｨ ｨｨ ｨｺ0502ｨｰ06ｨｺ, ｨ ｨｴｨｨ01 ｨｺｨ 0907ｨ 04ｨｮ 3, %, ｨｺ 05ｨｰ06ｨｬｨｮ ｨｺｨｮ, ｨｬ0603ｨｪ06, ｨ ｨｪ ｨｨｨ 0205ｨｹ06 ｨｰ0201 ｨｺ0502ｨｰ06ｨｺ, 09 ｨｺ06ｨｰ ｨｺｨ 0907ｨ 04ｨ 3 ｨ ｨｺｨｰｨｨ09ｨｨ080609ｨ 05ｨ 09ｨｹ ｨｪｨ ｨ ｨ ｨｪｨｪｨｨ ｨｺｨ 01 (8 ﾂ ｨｦ09ｨｰ09ｨｨ ). 0208ｨｮ00ｨｨｨｬ 06ｨ ｨｲ0709ｨｪ02ｨｪｨｨ02ｨｬ ｨｬ060302ｨｰ ｨ 04ｨｰｨｹ ｨ ｨｪ0707 ｨ ｨｺｨｰｨｨ09ｨ 02ｨｨ07 ｨ ｨｰ0604ｨ ｨｰｨ 0903ｨｨ ｨｺ0502ｨｰｨｺｨ 01 ｨｨ05ｨｨ ｨｬ02ｨｪｨｹ03ｨ 07 ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0609ｨｰｨｹ ｨｨ01 ｨｺ 0102ｨｦ09ｨｰ09ｨｨ06 ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ﾁ ｨ ｨ ｨｨ ﾂ ﾂ ｨ 08ｨ 0302ｨｪｨｨ ｨｰ06ｨｬｨｮ ｨｬ06ｨｬ02ｨｪｨｰｨｮ 09 04ｨ 08ｨ 0302ｨｪｨｪ0401 ｨｺ0502ｨｰｨｺｨ ｨｨ05ｨｨ ｨｺｨ 0907ｨ , 8 ｨｨ ｨｨｨｰ0205ｨｹｨｪ06 06ｨｺ08ｨ 0302ｨｪｨｪ0401 ｨｺ0502ｨｰ06ｨｺ ｨｰｨ ｨ , ｨｨ % ｨｰ0902ｨｰ09ｨｰ0902ｨｪｨｪ ｨｰ02 ﾂ02ｨｪｨｨ ｨｮ06ｨｴｨｨ01 24 ﾂ (48 ﾂ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｨ07) 09 04ｨ 08ｨ 0302ｨｪｨｪ06ｨｦ ｨｮ050702ｨｨｨｨ ﾁ, ｨｪ02 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ , ｨｨｨｰ0205ｨｹｨｪ06 06ｨｺ08ｨ 0302ｨｪｨｪ0401 ｨｺ0502ｨｰ06ｨｺ ｨｮ090205ｨｨ ﾂｨｨ05ｨ 09ｨｹ ｨｨ ｨｰｨｨ0005ｨ , ｨｨ % ｨｰ0902ｨｰ09ｨｰ0902ｨｪｨｪ06 (08ｨｨ09. 2, 01; 3) ｨｦ09ｨｰ09ｨｨ ｨｰ02 ﾂ02ｨｪｨｨ02 24 ﾂ 09 ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨｮ050702ｨｨｨｨ (48 ﾂ ｨ 08ｨ 0302ｨｪｨｨ07) ｨｺ0502ｨｰ06ｨｺ, ｨ ｨｴｨｨ01 ｨｺｨ 0907ｨ ｨｨ9,09ｨｪｨｨ04ｨｨ05ｨ 09ｨｹ ｨ 04ｨ, ｨｰｨ 09ｨｨ ｨｨ % ｨｰ0902ｨｰ09ｨｰ0902ｨｪｨｪ06 (04 <0.05) ｨｺ0502ｨｰ06ｨｺ, 06ｨｺ08ｨ 0302ｨｪｨｪ0401 ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｬｨｨ ｨｺ ｨｺｨ 0907ｨ , ｨｰｨ 09ｨｨ05ｨ % ｨｨ ｨ 0405ｨ 04ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪ06 ｨｬ02ｨｪｨｹ0302, ﾂ02ｨｬ 09 ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨｮ050702ｨｨｨｨ ｨ ｨｦ09ｨｰ09ｨｨ ｪ %(04 < 0.05) (08ｨｨ09. 3) ｨｪ06 ｨｺ06ｨｪ09ｨｰｨ ｨｰｨｨ080609ｨ ｨｰｨｹ, ﾂｨｰ06 ｨｺ0605ｨｨ ﾂ0209ｨｰ0906 ｨｺ0502ｨｰ06ｨｺ 09 ｨ ｨｺｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｪ04ｨｬｨｨ ｨｺｨ 0907ｨ 04ｨ ｨｬｨｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨｮ050702ｨｨｨｨ ﾁ 04ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪ06 ｨｮｨｬ02ｨｪｨｹ03ｨ 02ｨｰ ｨｨ ｨｦ09ｨｰ09ｨｨｨｨ ｨｪｨ 05ｨｰｨｨ ｨｺ0502ｨｰｨｺｨｨ ｨｮ ﾂ02ｨｪｨｪ ｨ ｨｪｨｪ ｨｺｨ ｨ 06ｨｰ, ﾂｨｰ06 ｨｨ , ｨｨ ｨｨｨｪ01ｨｮ02ｨｨ08ｨｮ06ｨｰ ｨ ｨｺｨｰｨｨ09ｨ 02ｨｨ06 ｨｺｨ 0907ｨ ｨ 09ｨｨ09ｨｨｨｬ ｨ ｨｰ0604ｨ. 00 ｨｰ ｨｬ07 ｨｪ02 ｨｨ09ｨｺ0506 ﾂ02ｨｪ06, ﾂｨｰ06 01ｨｨｨｪｨ ｨｬｨｨｨｺｨ ｨ ｨｰ0604ｨ 0708ｨｨ 05ｨｰｨｨ ｨｦ09ｨｰ09ｨｨ ｨ 0405ｨｨ ﾂｨ 02ｨｰ ｨｪｨ 09ｨｰ0607ｨｴ ｨｬ ｨｺｨ 04ｨ ｨｪ06, ﾂｨｰ06 00ｨｨｨ 0205ｨｹ ｨｺ0502ｨｰ06ｨｺ ｨｬ060302ｨｰ 0609ｨｮｨｴ0209ｨｰ090507ｨｰｨｹ ｨｨ ｨｹ040609ｨ ｨｪｨｨ02ｨｬ 08ｨ 04ｨｪ0401 ｨｬ060502ｨｺｨｮ050708ｨｪ0401 ｨｨ ｨｺ0502ｨｰ06 ﾂｨｪ0401 ｨｬ0201ｨ ｨｪｨｨ04ｨｬ0609, ｨｨ ｨｺ06ｨｰ ｨｪｨ ｨｮ 09 ｨ ｨｰ060406ｨｬ 06ｨｰｨｬ02 ﾂ02ｨｪｨ ｨ ｨｮｨｰ0600ｨ 00ｨｨ ｨｨ 09 05ｨｰｨｨｨｬ ｨｰｨ ｨｹ ｨｨｨｪｨｰ ｨｪ04ｨｬ ｨｪｨｨｨｰｨｹ 09ｨｺ05ｨ ｨ ｨｮｨｰ0600ｨ 00ｨｨｨｨ 09 00ｨｨｨ 0205ｨｹ ｨｺ0502ｨｰ06ｨｺ ﾁ ｨｦ09ｨｰ09ｨｨ02ｨｬ ｨｪｨｨ02 ｨｬｨ 08ｨｺ0208ｨ ｨ ｨｮｨｰ0600ｨ 00ｨｨｨｨ ｪ ｨ 0205ｨｺｨ LCP3A/B ｪ ｨｬ02ｨｰ060106ｨｬ ｨｨｨｬｨｬｨｮｨｪ0602ｨｨｨｰ0601ｨｨｨｬｨｨ ﾂ0209ｨｺ ｨ ｨｪｨ 05ｨｨ04ｨ 0706ｨｺｨ 04ｨ 0506, ﾂｨｰ06 ｨｺｨ ｨｺ 09 ｨｺ06ｨｪｨｰ080605ｨｹｨｪ0401 ｨｺ0502ｨｰｨｺｨ 01, ｨｰｨ ｨｺ ｨｨ 09 ｨｺ0502ｨｰｨｺｨ 01, 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ , 01ｨ ｨｪｨｪ04ｨｦ ｨ ｨｺ 0708ｨ ｨｺｨｰｨｨ ﾂ0209ｨｺｨｨ ｨｪ02 06ｨ ｨｪｨ 08ｨｮ03ｨｨ09ｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ｨｨｨ 0806ｨ 05ｨ 09ｨｰｨ 01 ﾂ ﾂ ｨ 08ｨ 0302ｨｪｨｨ ｨｺ0502ｨｰ06ｨｺ, 06ｨｺ08ｨ 0302ｨｪｨｪ0401 ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｬｨｨ ｨｺ ｨ 0205ｨｺｨｮ

6 130005ｨｨ07ｨｪｨｨ02 ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺｨ 0106ｨｺ090608ｨｮｨ ｨｨ02ｨｨｨｪｨ ｨｪｨ 00ｨｨｨ 0806ｨ 05ｨ 09ｨｰ04 ﾂ ｨｺｨ, ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ｨｨｨｰ06ｨｬ0200ｨ ｨｨ08ｨｮ0906ｨｬ 265 ｨｬ040302ｨ 01 ｨｬ040302ｨｦ ｨｦ09ｨｰ09ｨｨ02ｨｬ (Yu et al., 2014). 0208ｨｮ00ｨｨ02 ｨ 09ｨｰ ｨｪｨ ｨ ｨ 05ｨｨ ｨｮ090205ｨｨ ﾂ02ｨｪｨｨ02 ｨｮ080609ｨｪ07 05ｨｺ ｨｨｨｨ ｨ 0205ｨｺ0609, ｨｨｨｪ01ｨｮ02ｨｨ08ｨｮ02ｨｬ ｨｨ ｨ ｨｮｨｰ0600ｨ 00ｨｨｨｨ: 0708ｨｨ ｨｦ09ｨｰ09ｨｨｨｨ ｨｪｨ ｨｺ0502ｨｰｨｺｨｨ 0609ｨｰ020609ｨ 08ｨｺ06ｨｬ04 U2OSandSaos-2 (Zhao et al., 2014), ｨ ｨｰ06ｨｬ04 Hep3B (Chen et al., 2014) ｨｨ ｨｪｨ ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ08ｨｮ02ｨｬ0402 ｨｺ0502ｨｰｨｺｨｨ 08ｨ ｨｺｨ 07ｨｨ ﾂｨｪｨｨｨｺｨ (Fong et al., 2012). 04ｨ ｨｪｨｨ07 01ｨ ｨｪｨｪ0401 ｨｬ0600ｨｮｨｰ ｨ 04ｨｰｨｹ 06ｨ ｨｲ0709ｨｪ02ｨｪ04 08ｨ 04ｨｪ04ｨｬｨｨ 07ｨｮｨｰ07ｨｬｨｨ 06ｨｰ0902ｨｰｨ ｨｺ0502ｨｰ06ｨｺ ｨｪｨ 0102ｨｦ09ｨｰ09ｨｨ in vitro ｨｨ in vivo, 08ｨ 04ｨｪ04ｨｬｨｨ ｨｺ0502ｨｰ06 ﾂｨｪ04ｨｬｨｨ ｨｬ ｨｬｨｨ ｨｨ 01ｨ ｨ 04ｨｪ04ｨｬｨｨ 09ｨｨ01ｨ ｨｬｨｨ ｨｰｨｪ ｨｨ0906ｨｰｨｪ0401 (Dirks-Naylor, 2013). ﾁｨｰ06 ｨｺｨ 09ｨ 02ｨｰ ｨｨ07ｨｪｨｨ ｨｪｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ﾁ, ｨｰ06 ｨｬ0603ｨｪ ｨｨｨｰｨｹ, ﾂｨｰ06 09 ｨｮ ｨｨ0701 ｨｪｨ 03ｨｨ ｨｰ0609 ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺ ｨｪ02 06ｨｺｨ ｨ 02ｨｰ 0905ｨｨ07ｨｪｨｨ07 ｨｪｨ ｨ ｨｮｨｰ0600ｨ 00ｨｨ ﾁ, 06ｨｰｨｬ02 ﾂ02ｨｪｨｪｨｮ06 09 ｨ 0605ｨｹ03ｨｨｨｪ09ｨｰ ｨ 08ｨ 0302ｨｪｨｪ0401 ｨｺ0502ｨｰ06ｨｺ ｨｪｨ ｨｪｨｨ01 09ｨｰｨ 01ｨｨ0701 ｨｨｨｪ0002ｨｺ02ｨｨｨｨ. 0604ｨｬ02ｨｪ02ｨｪｨｨ02 ｨｮ080609ｨｪ07 ｨｬ ｨｨ ｨｬ ｨ 0205ｨｺｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ﾁ ｨ ｨｪｨｨ02 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ 0002ｨｪｨ ｨｬ02ｨｰ060106ｨｬ ｨｺｨ 04ｨ ｨｨ09ｨｮｨｰ09ｨｰ09ｨｨ02 09 ｨｪ02ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ ﾁ ｨｬ ｨｪ0608ｨ 04ｨｬ0208ｨｪ06ｨｦ ｨｨ ｨｬ04 ｨ 0205ｨｺｨ ｨｪｨ ｨｺ06 ｨｮ080609ｨｪｨｨ ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ ｨ 0405ｨｨ ｨｪｨｨ04ｨｺｨｨｨｬｨｨ (10 ｨC4 ｪ10 ｨC5 06ｨｰ ｨｮ080609ｨｪ07 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ ｨｪ09ｨｪ ｨｪｨ ) ｨｨ ｨｺ060502ｨ ｨ ｨｪｨｨ ｨｨｨｪｨ ｨｬｨｨｨｺ02 ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨ07 09 ｨｰ02 ﾂ02ｨｪｨｨ02 48 ﾂ ｨ 0405ｨｨ 09ｨｰｨ ｨｰｨｨ09ｨｰｨｨ- ﾂ0209ｨｺｨｨ ｨｪ0204ｨｪｨ ﾂｨｨｨｬ04 (08ｨｨ09. 4, ｨ ). 07ｨｺ ﾂ02ｨｪｨｪｨ 07 ｨｨ ｨｬｨ dn ｨ ｨｴ02 ｨｪ ｨ 09ｨｹ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ ﾁ ｨｮ0302 ﾂ ﾂ ｨ 08ｨ 0302ｨｪｨｨ07 ｨｮ ｨｪｨｹ ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ ｨｪ0608ｨ 04ｨｬ0208ｨｪ06ｨｦ ｨｨ ｨｬ ｨｮｨｴ0209ｨｰ0902ｨｪｨｪ ｨ (08ｨｨ09. 4, ｨ ) ﾂ ｨ 08ｨ 0302ｨｪｨｨ07 ｨｮ ｨｪｨｹ ｨ 05 ｨｮ ｨｪｨｹ 09 ｨｺ06ｨｪｨｰ ｪ20 08ｨ ｨｺｨｨｨｦ ｨｮ ｨｪｨｹ ｨｬ ｨｪｨ ｨ ｨ ｨｨ ｨｪｨ ｨｮ06ｨｴｨｨ ｨｺｨ ｨｰｨｹ ﾂ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｨ ｨ ｨｨｨｬ06 06ｨｰｨｬ02ｨｰｨｨｨｰｨｹ ｨｪｨｨ02 ｨｨ ｨｬ04 dn ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ﾁ, ｨｪ0209ｨｬ06ｨｰ0807 ｨｪｨ ｨｰ06 ﾂｨｰ06 ｨｮ ｨｪｨｹ ｨｬ ｨｰ06ｨｦ ｨｨ ｨｬ04 (ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨｨ ｨｨ08ｨｮ02ｨｬ06ｨｦ ｨｰ ｨｬ06ｨｰ0608ｨ, 08ｨ ｨｪｨｪ ｨ 05ｨｹｨｰ0208ｨｪｨ ｨｰｨｨ09ｨｪ06ｨｬ ｨｰ0802ｨｰｨｹ02ｨｬ ｨｨｨｪｨｰ0806ｨｪ02) 06ｨｰｨｪ0609ｨｨｨｰ0205ｨｹｨｪ06 ｨｪ02ｨ 0605ｨｹ0306ｨｦ. 08ｨｺｨｰｨｨ09ｨ 02ｨｨ07 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ 0002ｨｪｨ ｨｨ ｨｪｨｨ02 ｨｨ ｨｬ04 dn ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0401 ｨｪ0608ｨｬｨ 05ｨｹｨｪ0401 (ｨｪ020607ｨｮ ) ﾁ, ｨｪｨ 09ｨｺ0605ｨｹｨｺ06 ｨｪｨ ｨｬ ｨｨ ｨｰｨｪ06, 0706ｨｺｨ 04ｨ ｨｪ04 ｨｪｨ ｨｬｨｨ ｨｨ ｨｬ02ｨｪ02ｨｪｨｨ02 05ｨｺ ｨｨｨｨ 0002ｨｪｨ TP73 09 ｨｺ0502ｨｰｨｺｨ ﾁ ｨｪｨ 08ｨ 04ｨｪ ｨｰｨ 01ｨｨ ｨｨｨｪ0002ｨｺ02ｨｨｨｨ ｨｨ0406ｨｪｨｰｨ 05ｨｨ ｪ ｨｬ07 ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨ07, ﾂ; ｨｰｨｨｨｺｨ 05ｨｨ ｪ 06ｨｰｨｪ060302ｨｪｨｨ02 ｨｮ080609ｨｪ02ｨｦ ｨｨ ｨｬ ｨｨ00ｨｨ ﾂ0209ｨｺｨｨ01 ｨｬ ｨｪｨ TP73 ｨｨ ｨｪ09ｨｪ ｨｪｨ GAPDH. ｨ ｪ ｨｺ06ｨｪｨｰ080605ｨｹｨｪ0402 ｨｺ0502ｨｰｨｺｨｨ, 06ｨｰ09 ﾂ02ｨｰ ｨｬ02ｨｪｨｨ ｨｪｨ ﾂｨｨｨｪｨ 05ｨｨ ﾂ ﾂ ｨｪｨ ﾂｨ 05ｨ ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨ07; ｨ ｪｨｺ0502ｨｰｨｺｨｨ ｨｪｨ 08ｨ 04ｨｪ ｨｰｨ 01ｨｨ ｨ 08ｨ 0302ｨｪｨｨ LCP3A/B, ｨｪｨ ｨｨ05ｨ 09ｨｹ ｨｪｨ ｨｮ080609ｨｪ02 ｨｺ06ｨｪｨｰ080605ｨｹｨｪ06ｨｦ ｨｮ050702ｨｨｨｨ ｨｨ ｨｪ ｨ 05ｨ %. 0201ｨｪｨ ｨｺ06 ﾂ ﾂ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｨ07 ｨｬｨ 08ｨｺ0208 ｨ ｨｮｨｰ0600ｨ 00ｨｨｨｨ ｨｨ05ｨｨ ｨｮ % ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0401 ｨｺ0502ｨｰ06ｨｺ (08ｨｨ09. 2, 03). 0202ｨｦ09ｨｰ09ｨｨ ｨｪｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ﾁ ｨｪ02 ｨｨ04ｨｬ02ｨｪｨｨ LCP3A/B ｨｨｨｰ0205ｨｹｨｪ0401 ｨｺ0502ｨｰ06ｨｺ. 06ｨ ｨｺｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ, ｨｬ0603ｨｪ ｨｨｨｰｨｹ, ﾂｨｰ ｨｨｨ 0205ｨｨ ﾁ (ｨｪ02 ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ) ｨｦ09ｨｰ09ｨｨ02ｨｬ ｨｮ ｨｨ0701 ｨｪｨ 03ｨｨ ｨｰ0609 ｨｮ ﾂｨ 09ｨｰ09ｨｮ06ｨｰ ｨ ｨｰ0604ｨ ｨｨ ｨｪ ﾂ02ｨｪ04 ｨｬ0201ｨ ｨｪｨｨ04ｨｬ04 ｨ ｨｮｨｰ0600ｨ 00ｨｨｨｨ 09 ｨｮ ﾂｨ 09ｨｰｨｨ02ｨｬ ｨ 0205ｨｺｨ LCP3A/B ｨｮ05ｨｹｨｰｨ ｨｰ ｨｮｨ 05ｨｨｨｺ0609ｨ ｨｪｨｪ ｨ ｨ 06ｨｰ 06ｨ ｨｮ ﾂｨ 09ｨｰｨｨｨｨ ｨ ｨｮｨｰ0600ｨ 00ｨｨｨｨ 09 06ｨｰ0902ｨｰ02 ｨｪｨ ｨｰｨｨ ﾂｨｨ ｨ 03ｨｨ 01ｨ ｨｪｨｪ ｨ 09ｨｮ06ｨｰ ｨｮ05ｨｹｨｰｨ ｨｰｨ ｨｬｨｨ ｨ 09ｨｰ , ｨｪ02 06ｨ ｨｪｨ 08ｨｮ03ｨｨ0903ｨｨ01 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨｨｦ 09 05ｨｺ ｨｨｨｨ 0707ｨｰｨｨ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ0401 ｨｨｨｬｨｨ ｨｬｨ 08ｨｺ ｨ ｨｮｨｰ0600ｨ 00ｨｨｨｨ 09 09ｨｺ020502ｨｰｨｪ ｨ 09ｨｮ030102ｨｪｨｨ ｨｺｨｮ07ｨｪ0609ｨｰｨｹ ｨｮ ﾂ02ｨｪｨｪ ｨ ｨｪｨｪ0401, 06ｨ 06ｨ ｨｴ02ｨｪｨｪ ｨｰｨ ｨ 05ｨｨ0202, 0706ｨｺｨ ｨ 02ｨｰ, ﾂｨｰ ｨｨ 0906 ﾂ02ｨｰｨ ｨｪｨｪ06ｨｬ 0102ｨｦ09ｨｰ09ｨｨｨｨ ｨｨ ｨｮｨｬ02ｨｪｨｹ03ｨ 02ｨｰ ｨｨｨ 0205ｨｹ ｨｺ0502ｨｰ06ｨｺ ﾁ, 09ｨｪｨｨ03ｨ 02ｨｰ0907 ｨｺ0605ｨｨ ﾂ0209ｨｰ0906 ｨｺ0502ｨｰ06ｨｺ ｨｪｨｪ06ｨｦ ｨｨ ｨｪ02 ｨｪｨ ｨ ｨ 02ｨｰ0907 ｨ 0506ｨｺｨ ｨｬｨｨｨｰ0604ｨ ｨ 09ｨｪ02ｨｪｨｨ06 09 ｨｪ0204ｨ 08ｨ 0302ｨｪｨｪ04ｨｬｨｨ ｨｺ0502ｨｰｨｺｨ ｨｬｨｨ, 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ04ｨｬｨｨ ｨｪ ｨｨｨｰｨｹ, ﾂｨｰ ｨ ｨｰｨｰ02ｨｪｨｮｨｨ08ｨｮ02ｨｰ ｨ 06ｨｴ ｨｦ09ｨｰ09ｨｨ ｨｪｨ ｨｪ0608ｨｬｨ 05ｨｹｨｪ0402 ｨｺ0502ｨｰｨｺｨｨ ｨｺｨ ｨ 02ｨｰ ｨｰｨｨ ｨｮ ｨｦ09ｨｰ09ｨｨ02, ｨ ｨ ｨｺ060906ｨｦ ｨｺ0502ｨｰｨｺｨｨ. 00 ｨｰ ｨｬ ｨｪ06ｨｰ06ｨｺ09ｨｨ ﾂ0209ｨｺｨｨｨｦ ｨｺｨｰ ｨｺｨ ｨ 02ｨｰ ｨｰｨ ｨｺ0302 ｨｪｨ 0108ｨｮ00ｨｨ02 ｨｺ0502ｨｰｨｺｨｨ ｨ ｨｪｨｨ04ｨｬｨ, 09 ｨｰ06ｨｬ ﾂｨｨ ｨ, 09 ｨｰｨｺｨ ｨｪｨｨ ｨｺ06ｨｰ ｨｨ04ｨｬ02ｨｪ0702ｨｰ ｨｺ ｨｨ ｨｪ0609, ｨｮ ﾂｨ 09ｨｰ09ｨｮ06ｨｴｨｨ ｨｪｨｨｨｨ ｨｨ ｨ 08ｨ 02ｨｨｨｨ (Manjanatha et al., 2014). 05ｨｪ07ｨｰｨｨ ｨ 06ｨｴ ｨｦ09ｨｰ09ｨｨ ｨｪｨ ｨｪ0608ｨｬｨ 05ｨｹｨｪ0402 ｨｺ0502ｨｰｨｺｨｨ ｨ ｨｪｨｨ04ｨｬｨ ｪ 09ｨ 03ｨｪｨ 07 04ｨ 01ｨ ﾂｨ, ｨｪｨｨ02 ｨｺ06ｨｰ060806ｨｦ 04ｨ 09ｨｨ09ｨｨｨｰ 06ｨｰ ｨｪｨ ｨｪｨｨ07 00ｨ ｨｺｨｰ , ｨｺ06ｨｰ ｨｨ05ｨｨ ｨ ｨｰ0908ｨ ｨｰｨｨｨｰｨｹ 00ｨｨｨ 0205ｨｹ ｨｪ0608ｨｬｨ 05ｨｹｨｪ0401 ｨｺ0502ｨｰ06ｨｺ, ｨ ｨｪｨｨ ｨｰｨｨ ｨｮ ｨｮ06 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ ｨ ｨｪｨｰｨｨｨ ｨｨ06ｨｰｨｨｨｺｨ. 01ｨ 0708ｨ ｨｪｨｨ ｨｨ09ｨｺｨ ｨｰｨ ｨｺｨｨ01 00ｨ ｨｺｨｰ ｨｬ060302ｨｰ ｨ 04ｨｰｨｹ ｨｪｨ ｨｦ0102ｨｪ ｨｨ ｨｨ04ｨｮ ﾂ02ｨｪｨｨｨｨ ｨｨｨｪ0002ｨｺ02ｨｨｨｨ ｨｨ ｨｰｨｨ090609ｨｨ08ｨｮ09ｨｪ ｨｰ0902ｨｰｨ ｨｺ0502ｨｰｨｺｨｨ.

7 ｨ ｨｨ ｨｨ07ｨｪｨｨ ｨｪｨ ﾁ, ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0402 ｨｨ ｨｪ02ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ｨｰｨｺｨｨ ｨ ﾁｨｨ ｨｺ0502ｨｰ06ｨｺ ｨｪｨｨ07 ﾁｨｨ ｨｬｨｨｨｰ , TUNEL ｨｨｨｰ0205ｨｹｨｪ0401 ｨｺ0502ｨｰ06ｨｺ, % ｨｺ0502ｨｰ06ｨｺ (%), ｨ ｨｴｨｨ01 ｨｺｨ 0907ｨ 04ｨｮ 3 ｨｺｨ 0907ｨ 04ｨｮ 8 ｨｺｨ 0907ｨ 04ｨｮ 9 LC3A/B ｨC ｨC ｨC ｨC ｨ ｨ ｨ ｨ ｨ ｨ ｨ ｨ ｨ ｨ ｨ ｨ ｨ ｨ ｨ ｨ ｨｰｨｺｨｨ ﾁ ﾂ ﾂ ｨ 01ｨｺｨｨ 04ｨ 08ｨ 03ｨ 05ｨｨ ﾁ ｨ 08ｨ 0302ｨｪｨｨ07 09ｨｪ0609ｨｨ05ｨｨ ｨｨ ｨｨｨｪｨｺｨｮｨ ｨｨ080609ｨ 05ｨｨ ｨｮ06ｨｴｨｨ02 24 ﾂ. 0806ｨｪｨｰ080605ｨｹｨｪ0402 ｨｺ0502ｨｰｨｺｨｨ (000000ｨC ｨC) ｨ ｨｪｨ 05ｨｨ04ｨｨ080609ｨ 05ｨｨ 09 ｨｺ06ｨｪ ｨｰｨ ( ﾂ ﾂ ｨ 01ｨｺｨｨ). ｨ 05ｨｪｨ ﾂ02ｨｪｨｨ07, 06ｨｰ05ｨｨ ﾂｨ 06ｨｴｨｨ ｨｰ ｨｺ06ｨｪｨｰ080605ｨｹｨｪ0401 (000000ｨC ｨC) 09 ｨｺｨ ｨｬ 09ｨｰ0605ｨ 0202 (04 < 0.05, ｨｺ08ｨｨｨｰ0208ｨｨｨｦ 05ｨｰｨｹ060102ｨｪｨｰｨ ) ｨｰｨｪ06, ﾂｨｰ06 ｨｪｨ 08ｨ ｨｪｨｪｨｨ01 09ｨｰｨ 01ｨｨ0701 ｨｨｨｪ0002ｨｺ02ｨｨｨｨ ｨ 02ｨｪ ｨｰ09ｨｰ090609ｨ ｨｰｨｹ 08ｨ 0409ｨｨｨｰｨｨ06 ｨ ｨｰ0604ｨ, ｨ ｨｪ ｨｨ04ｨｪ ｨ ｨｪ0609ｨｰｨｹ ｨｺ0502ｨｰ06ｨｺ ｨｺ06ｨｪ ﾂｨ ｨｪｨｨ ｨｨｨｺｨ ｨｰｨｨ09ｨｪ ｨｨｨｺ05ｨ ｨｪ06ｨｬｨ. 0306ｨｺｨ 04ｨ ｨｪ06, ﾂｨｰ ｨｪ ｨｮ ﾂｨ 09ｨｰ09ｨｮ02ｨｰ ｨｰｨｨ ｨｦ09ｨｰ09ｨｨｨｨ ｨ ｨｰ0604ｨｮ, 09 ｨｰ06ｨｬ ﾂｨｨ UL36 (vica), ｨｺ06ｨｰ060804ｨｦ ｨ ｨｰ ｨ ｨｺｨｰｨｨ09ｨ 02ｨｨ ｨｺｨ 0907ｨ ; UL37 (vmia) ｪ ｨｬｨｨｨｰ060106ｨｪ0108ｨｨｨ 05ｨｹｨｪ04ｨｦ ｨｨｨｪ00ｨｨｨ ｨｨｨｰ0608 ｨ ｨｰ0604ｨ, ｨｺ06ｨｰ060804ｨｦ 0904ｨ ｨｨｨｬ060102ｨｦ09ｨｰ09ｨｮ02ｨｰ 09 ｨ 0205ｨｺ06ｨｬ Bax ｨｨ ｨｰ0908ｨ ｨｴｨ 02ｨｰ ｨｬ02ｨ ｨ ｨｨ05ｨｨ04ｨ 02ｨｨ06 ｨｬｨｨｨｰ060106ｨｪ0108ｨｨｨ 05ｨｹｨｪ06ｨｦ ｨｬ02ｨｬｨ 08ｨ ｨｪ04 (Goldmacher, 2005). 00 ｨｺ0502ｨｰｨｺｨ 01, 04ｨ 08ｨ 0302ｨｪｨｪ , ｨｪｨ 08ｨ ｨｪｨｪｨｨ01 09ｨｰｨ 01ｨｨ0701 ｨｪ ｨｨ ｨｨｨｰ 08ｨ , ｨｬ0401 ｨｬ02ｨｰ060106ｨｬ TUNEL (Chiou et al., 2001; ｨ ｨｨ 0108., 2008), ｨ ｨｰｨ ｨｺ ｨ ｨｪｨ 08ｨｮ0302ｨｪ04 ｨ 0205ｨｺｨｨ, ｨ ｨｪ0706ｨｴｨｨ ｨｰｨｪ0609ｨｰｨｹ ｨｺ0502ｨｰ06 ﾂｨｪ06ｨｦ ｨｨ ｨｨｨｪ0002ｨｺ02ｨｨｨｨ (Fortunato, Spector, 1998). 0207ｨｮｨ 05ｨｨｨｺ0609ｨ ｨｪ04 01ｨ ｨｪｨｪ ｨｰ06ｨｬ, ﾂｨｰ ｨｦ09ｨｰ09ｨｨ02ｨｬ ｨ 02ｨｰ0907 ｨｮ09ｨｰ06ｨｦ ﾂｨｨ090609ｨｰｨｹ ｨｺ0502ｨｰ06ｨｺ ｨｺ 08ｨ 0405ｨｨ ﾂｨｪ04ｨｬ 00ｨ ｨｺｨｰ0608ｨ ｨｬ, ｨｨｨｪ01ｨｮ02ｨｨ08ｨｮ06ｨｴｨｨｨｬ ｨ ｨｰ0604, 09 ｨｰ06ｨｬ ﾂｨｨ ｨｺ 02ｨｨｨｰ06ｨｰ06ｨｺ09ｨｨ- ﾂ0209ｨｺｨｨｨｬ 0902ｨｴ0209ｨｰ09ｨ ｨｬ, ｨｰｨ ｨｺｨｨｨｬ ｨｺｨ ｨｺ ｨ ｨｺｨｰｨｨｨｪ06ｨｬｨｨ02ｨｨｨｪ 02 ｨｨ 02ｨｨ090705ｨ ｨｰｨｨｨｪ (Cinatl et al., 1998; Terrasson et al., 2005) ｨｪ ｨｬ07 09ｨｪｨｨｨｬｨ ｨｪｨｨ02 ｨｨ ｨ ｨｰ020502ｨｦ 0708ｨｨ090502ｨｺｨ 02ｨｰ ｨ ｨｺ 0873, 0002ｨｪ ｨｺ06ｨｰ ｨｨｨｬ0202ｨｰ 04ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪ ｨｰ ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ07ｨｬｨｨ ｨｰ06ｨｰｨｨ07ｨｪ ｨｮ ｨｮ ｨ ｪ ｨ 0205ｨｺｨ ｨｰ05ｨｨ ﾂｨｨｨｰ0205ｨｹｨｪ06ｨｦ ｨ 02ｨｪｨｪ0609ｨｰｨｹ06 ｨ 0205ｨｺｨ ｨｰ ｨ 08ｨ ｨ ｨｪｨｨ02 ｨ 0605ｨｹ ｨｺ0605ｨｨ ﾂ0209ｨｰ09ｨ C- ｨｨ N-ｨｺ06ｨｪ ｨｨ ｨｬ ｨｺｨ ｨｺ ｨｮ05ｨｹｨｰｨ ｨｰ ｨｨ ｨｹ040609ｨ ｨｪｨｨ07 ｨ 05ｨｹｨｰ0208ｨｪｨ ｨｰｨｨ09ｨｪ ｨ ｨｦ09ｨｨｨｪ00ｨ ｨｨ ｨ 05ｨｹｨｰ0208ｨｪｨ ｨｰｨｨ09ｨｪ ｨｬ06ｨｰ0608ｨ ｨｨ ｨｨ ｨｬ ｨ 0205ｨｺｨ ｨｪｨｨｨｦ ｨｪ ｨｰｨ ｨｰ06 ﾂｨｪ06, ｨｪ06 01ｨ ｨｪｨｪ ｨ ｨ ｨｪｨｰｨ 0006ｨｪｨｨ09ｨｰｨｨ ﾂ0209ｨｺｨｨ01 00ｨｮｨｪｨｺ02ｨｨ0701 ｨｪ02ｨｺ06ｨｰ ｨｨ ｨｬ ｨｨ05ｨｨ ｨｨ ｨ ｨｰ020507ｨｬ ｨｰｨ 09ｨｨｨｰｨｹ : ﾂ02ｨｬ ｨｰ ｪ 0607ｨｮ ｨｬ 09ｨｮ ｨｬ ｨｨ05ｨｨ 06ｨｪｨｺ060002ｨｪ06ｨｬ (Stiewe, Pｨｹtzer, 2002)? 00ｨ 0506 ｨｨ ｨｰｨｪ06 ｨｨ 06ｨ ｨｮ ﾂｨ 09ｨｰｨｨｨｨ ｨｨ ｨｬ ｨｮ050702ｨｨｨｨ ｨｨ000208ｨ 02ｨｨｨｨ ｨｨ 00ｨｨｨ 0205ｨｨ ｨｪ0608ｨｬｨ 05ｨｹｨｪ0401 ｨｺ0502ｨｰ06ｨｺ, 09 ｨｰ06ｨｬ ﾂｨｨ ｨｦ09ｨｰ09ｨｨ02ｨｬ ｨｨ04ｨｬ0208ｨｨ05ｨｨ ｨｮ080609ｨｪｨｨ ｨｬ ｨｮ01 ｨｨ ｨｬ ｨ 0205ｨｺｨ 0873 ｪ ｨｨ dnp73 ｪ ﾁ ｨｨ ｨ ｨｪｨ 05ｨｨ04ｨｨ080609ｨ 05ｨｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ ｨｪｨｨ07 ｨｬｨ 08ｨｺ ｨ ｨｬｨｬｨｨ080609ｨ ｨｪｨｪ06ｨｦ 00ｨｨｨ 0205ｨｨ ｨｺ0502ｨｰ06ｨｺ ｪ ｨ ｨｺｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｪ0401 ｨｺｨ 0907ｨ ｨｮ ﾂ02ｨｪｨｪ ｨ ｨｪｨｪ ｨｺｨ 04ｨ 05ｨｨ, ﾂｨｰ06 ｨｮ ｨｪｨｹ ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ ｨｨ ｨｬ ｨｪ02ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ﾁ 0106ｨｰ07 ｨｨ ｨｪｨｨ0406ｨｺ, ｨｪ ｨｰｨ ｨｰ06 ﾂ02ｨｪ ｨｨｨｪ01ｨｮｨｺ02ｨｨｨｨ ｨ ｨｰ0604ｨ ｨｦ09ｨｰ09ｨｨ02ｨｬ ｨｰｨｪ06, ﾂｨｰ ｨｰ0902ｨｰ ｨｪｨ ｨｪｨｨ ｨｰ08ｨ ｨｪ09ｨ ｨｺｨｰｨｨ09ｨｨ08ｨｮ06ｨｴｨ 07 00ｨｮｨｪｨｺ02ｨｨ07 ｨ 0205ｨｺｨ ｨ 02ｨｰ ｨｪ0609ｨｪ06ｨｬ ｨｪ02 ﾂ ｨ ｨｺｨｰｨｨ09ｨ 02ｨｨ06 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ ｨｪｨ, ｨ ｨｪｨ ｨ 0205ｨｺ060906ｨｬ ｨｮ080609ｨｪ ｨｬ06ｨｴｨｹ06 ｨｬｨｪ ﾂｨｨ090502ｨｪｨｪ ｨｰｨｰ08ｨ ｨｪ ｨｨ06ｨｪｨｪ0401 ｨｬ0601ｨｨ00ｨｨｨｺｨ 02ｨｨｨｦ ｨｬ02 ｨｰ060006, 0706ｨｺｨ 04ｨ ｨｪ06, ﾂｨｰ06 ｨｨ ｨｬｨ ｨｪ0206ｨ ｨｨｨｬｨ ｨｺ06ｨｪｨｰｨ ｨｺｨｰｨｪ ｨｰ0608ｨｬ060302ｨｪｨｨ ｨｰｨ ｨｺ0502ｨｰ06ｨｺ (Beitzinger et al., 2008) ｨｹ ｨ 0205ｨｺｨ ｨｰ0908ｨ ｨｴ02ｨｪｨｨｨｨ ｨｺ06ｨｪｨｰｨ ｨｺｨｰｨｪ06-ｨｪ0204ｨ 09ｨｨ09ｨｨｨｬ06ｨｦ ｨｨ000208ｨ 02ｨｨｨｨ ｨ ｨｪｨ ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨ06ｨｪｨｪ06ｨｦ ｨ ｨｬｨｬ06ｨｦ (06ｨｰ05ｨｨ ﾂｨｪ06ｨｦ 06ｨｰ 0853), ｨ ｨｨｨｬ02ｨｪｨｪ06 09 ｨｪｨ ｨｴｨｨｨｬｨｨ ｨｺ06ｨｪｨｰ ｨｬ ｨｬ02ｨｬｨ 08ｨ ｨｪｨｪ04ｨｬｨｨ ｨ 0205ｨｺｨ ｨｬｨｨ FAM38B ｨｨ KCNK1. 02ｨｰｨｬ02 ﾂ02ｨｪｨｪ0602 ｨｪ0204ｨｪｨ - ﾂｨｨｨｰ0205ｨｹｨｪ ｨｪｨｨ02 ｨｮ080609ｨｪ07 ｨｬ ｨｨ ｨｬ ｨｺ 48 ﾂ ｨｬ060302ｨｰ ｨ 04ｨｰｨｹ ｨ ｨｪ06 09 ｨｮ0302 ｨｪｨ ﾂｨ 0903ｨｨｨｬ0907 ｨｰ0608ｨｬ060302ｨｪｨｨ02ｨｬ ｨｺ0502ｨｰ06 ﾂｨｪ06ｨｦ ｨｨ000208ｨ 02ｨｨｨｨ (72 ﾂ ｨｪｨ ﾂｨ 05ｨ ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨ07). 0508ｨ 09ｨｪｨｨｨｰ0205ｨｹｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ04 ｨｮ080609ｨｪ02ｨｦ ｨｬ ｨｨ ｨｬ ｨ 0205ｨｺｨ ｨｺｨ 04ｨ 05, ﾂｨｰ ﾁ, ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ , 04ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪ ｨ 02ｨｰ0907 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨ ｨｪｨ , 0708ｨｨ ﾂ02ｨｬ 09 06ｨ 06ｨｨ ｨｬ06ｨｰ ｨｨ09ｨｺ0506 ﾂ02ｨｪ06, ﾂｨｰ06 ｨｮ090205ｨｨ ﾂ02ｨｪｨｨ02 ｨｮ080609ｨｪ07 ｨｬ ｨｨ ｨｬ ｨｰ06ｨｦ 09ｨｨｨｰｨｮｨ 02ｨｨｨｨ ｨｰ ｨｪｨｨ02ｨｬ ｨｮ0507ｨｰ0608ｨｪ ｨｬ0201ｨ ｨｪｨｨ04ｨｬｨ, ｨｰ0908ｨ ｨｴｨ 06ｨｴ ｨｪｨｨ02 ｨ ｨ ｨ ｨｪｨｰｨｪ0401 ｨｬｨｨｨｰ ｨｨ ｨｪ0209ｨｰｨ ｨ ｨｨ05ｨｹｨｪ0609ｨｰｨｨ 0002ｨｪ06ｨｬｨ (Talos et al., 2007) ｨｬ0603ｨｪ06, 09 05ｨｰｨｨｨｬ ｨ ｨｪ06 06ｨｰ09ｨｮｨｰ09ｨｰ09ｨｨ02 ｨｪｨ ｨｺ ｨｪｨｨ07 ｨｺ0502ｨｰ06ｨｺ 09 ｨｬｨｨｨｰ060402, ｨｪｨ ｨ ｨ ｨｪｨ ｨｬｨｨ 0708ｨｨ 0102ｨｦ09ｨｰ09ｨｨｨｨ ｨｪｨ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ ﾁ ｨｪｨｨ02 ｨｮｨｺ ﾂ02ｨｪｨｪ06ｨｦ ｨｨ ｨｬ04 dn ｨｰｨ ｨｰ ｨ 04ｨｦ ｨｨｨｪｨｰ , ｨｰｨ ｨｺ ｨｺｨ ｨｺ 05ｨｰｨ ｨｨ ｨｬｨ, 0904ｨ ｨｨｨｬ060102ｨｦ09ｨｰ09ｨｮ , ｨｨｨｪ00ｨｨｨ ｨｨ08ｨｮ02ｨｰ 0202 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨ06ｨｪｨｪｨｮ06 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ, ﾂｨｰ ﾂ ｨｹ ｨｨｨｪ00ｨｨｨ ｨｨ08ｨｮ02ｨｰ ｨｨｨｪ01ｨｮ02ｨｨ080609ｨ ｨｪｨｪ04ｨｦ ｨ ｨｰ ｨｰ060806ｨｦ ｨｬ0201ｨ ｨｪｨｨ04ｨｬ, ｨｬ06ｨｴｨｹ06 ｨｺ06ｨｰ ｨｰｨ ｨｮｨｺ ﾂ02ｨｪｨｪｨ 07 ｨｨ ｨｬｨ ｨ 0205ｨｺｨ 0873 ｨｬ060302ｨｰ ｨｮ090205ｨｨ ﾂｨｨ09ｨ ｨｰｨｹ ｨｮ09ｨｰ06ｨｦ ﾂｨｨ090609ｨｰｨｹ ｨｺ0502ｨｰ06ｨｺ ｨｺ , ｨｬ060302ｨｰ ｨ 04ｨｰｨｹ ｨ ｨｪ 09 ｨｪ0201ｨ 09ｨｪ06 06ｨｰｨｺ0804ｨｰ06ｨｦ ｨｪ060906ｨｦ 00ｨｮｨｪｨｺ02ｨｨ02ｨｦ ｨ 0205ｨｺｨ dn0873 (Wilhelm et al., 2010). 0306ｨｺｨ 04ｨ ｨｪ06, ﾂｨｰ06 dn ｨｺｨ 05ｨｨ04ｨｮ02ｨｰ0907 ｨｪ ｨｰ0902ｨｪｨｪ06 ｨｪｨ 09ｨ ｨｦｨｰ ｨｪｨｨ , 0904ｨ ｨｨｨｬ060102ｨｦ09ｨｰ09ｨｮ02ｨｰ ｨｪ090608ｨｪ04ｨｬ ｨ 0205ｨｺ06ｨｬ ｨｨ ｨｨｨｪ00ｨｨｨ ｨｨ08ｨｮ02ｨｰ ｨ ｨｺｨｰｨｨ09ｨ 02ｨｨ ｨｨ ｨｮ06ｨｴ ｨｨ05ｨｨ080609ｨ ｨｪｨｨ , ｨｰｨ ｨｺｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ 0905ｨｨ0707 ｨｨ ｨｪｨ ｨ 09ｨｨ09ｨｨｨｬ04ｨｦ ｨ ｨｰ ｨ ｨ ｨ 02ｨｬｨ 07 ｨｪｨ ｨｬｨｨ ｨ ｨｺｨｰｨｨ09ｨ 02ｨｨ07 ｨｺｨ 0907ｨ ｨ 0605ｨｹ03ｨｨｨｪ09ｨｰ ｨｨｨｪ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0401 ｨｺ0502ｨｰ06ｨｺ ｨ 05ｨ 09ｨｹ 00ｨｨｨ 0205ｨｹ06 ｨｺ0502ｨｰ06ｨｺ, 0601ｨｪｨ ｨｺ06 ｨｪ02 09ｨｰ0605ｨｹ ｨｬｨ ｨｦ, ｨｺｨ ｨｺ 0708ｨｨ 0102ｨｦ09ｨｰ09ｨｨｨｨ (09ｨｬ. ｨｰｨ ｨ 05ｨｨ02ｨｮ) ｨｪ ｨｨｨｰｨｹ, ﾂｨｰ06 09 ｨｬ0201ｨ ｨｪｨｨ04ｨｬ 00ｨｨｨ 0205ｨｨ ﾁ 09ｨｺ0506 ﾂｨ 02ｨｰ0907 ｨｨｨｪ01ｨｮｨｺ02ｨｨ07 05ｨｺ ｨｨｨｨ ｨｨ ｨｬ04 dn ｨｪｨｨ02 ｨｮｨｺ ﾂ02ｨｪｨｪ06ｨｦ ｨｨ ｨｬ ｨ 02ｨｰ ｨｮ ｨｨ ｨ ｨｰｨｰ02ｨｪｨｮｨ 02ｨｨｨｨ 0905ｨｨ07ｨｪｨｨ ｨｪｨ ｨｪｨｨ ｨｨ 00ｨｨｨ 0205ｨｹ ｨｺ0502ｨｰ06ｨｺ. 0604ｨｮ ﾂ02ｨｪｨｪｨ 07 ｨｺ0502ｨｰ06 ﾂｨｪｨ 07 09ｨｨ09ｨｰ02ｨｬｨ 06ｨｰｨｺ080409ｨ 02ｨｰ ｨｺｨｰｨｨ09ｨｮ 0706ｨｨ09ｨｺｨ 00ｨ ｨｺｨｰ , 09ｨｪｨｨ03ｨ 06ｨｴｨｨ01 ｨｰ06ｨｺ09ｨｨ ﾂ0209ｨｺ ｨｦ09ｨｰ09ｨｨ ｨｪｨ ｨｪ0608ｨｬｨ 05ｨｹｨｪ0402 ｨｺ0502ｨｰｨｺｨｨ ｨ ｨｪｨｨ04ｨｬｨ.