MC iosep ro duc CONTENTS CO NTENTS 7

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "MC iosep ro duc CONTENTS CO NTENTS 7"

Transcription

1 PB-9 Proteins, Peptides & Nucleic acids Ion Ex change Chr omat atog ogra raph phy Size Exclusion Chr omat ography Re verseded Phase Chr om at ogra phy Normal Phase Chr hrom omat og raph y

2 MC iosep ro duc CONTENTS CO NTENTS 7

3 Column Selection Guide

4 Proteins, Peptides, Saccharides IgG Transferrin Albumin N8E min Eluent : A) mm Tris-HCl (ph 8.6) B) mm Tris-HCl (ph 8.6) containing. M NaCl -%B (- min), -%B (- min) Flow rate :. ml/min Temperature : C Detection : UV at 8 nm Injection : L ( L/mL) N866A 6 min Eluent :. M KHPO-KHPO (ph 7.) containing. M NaCl Flow rate :. ml/min Temperature : ambient ( C) Detection : UV at 8 nm Injection : L ( L/mL) IgG Albumin Transferrin P8A NaN Mouse monoclonal IgG (Anti-human IgG) min P8A Mouse monoclonal IgG (Anti-human IgG) NaN min Eluent : A) mm Tris-HCl (ph 8.) B) mm Tris-HCl (ph 8.) containing. M NaCl - %B (-8 min) Flow rate :. ml/min Temperature : C Detection : UV at nm Injection : L (. mg/ml) Eluent :. M KHPO-KHPO (ph 7.) Flow rate :.7 ml/min Temperature : ambient ( C) Detection : UV at nm Injection : L (. mg/ml). Rabbit IgG. Mouse IgG Fc fragment Mouse IgG Fc fragment R86D min Eluent :. M KHPO-KHPO (ph 6.9) containing. M NaCl Flow rate :. ml/min Temperature : ambient (7 C) Detection : UV at nm Injection : L (. mg/ml) R869B min Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -%B (- min) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm Injection : L (. mg/ml) YMC Bioseparation Products

5 N8B 6 min 6 N86L * 6 min N88B 6 min Eluent : A) mm Tris-HCl (ph 8.6) B) mm Tris-HCl (ph 8.6) containing. M NaCl -%B (- min), -6%B (-6 min) Flow rate :. ml/min Temperature : C Detection : UV at nm Injection : L Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -%B (- min), -%B (- min), %B (-6 min) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm Injection : L * undigested BSA * * MW. Myoglobin 7,). Insulin (Bovine),7). Neurotensin,67). Tetraglycine 6). Glycine ) Eluent :. M KHPO-KHPO (ph 7.) containing. M NaCl/acetonitrile (7/) Flow rate :.7 ml/min Temperature : ambient ( C) Detection Injection : UV at nm : L min min G967A G96A Eluent : methanol/ mm NHHPO (/9) Flow rate :. ml/min Temperature : 7 C Detection : FLS at Ex. nm, Em. nm Injection : L (. pmol/ml) Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD. Eluent : methanol/ mm NHHPO (8/) Flow rate :. ml/min Temperature : 7 C Detection : FLS at Ex. nm, Em. nm Injection : L (. pmol/ml) Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD.

6 Nucleic acids N8G min Eluent : A) mm Tris-HCl (ph 8.) containing.7 M NaCl B) mm Tris-HCl (ph 8.) containing. M NaCl -%B (- min) Flow rate :. ml/min Temperature : C Detection : UV at 6 nm Injection : L Plasmid pbr Hae digest (8-87 bp) Plasmid pbr (,6 bp) N86F min Eluent : A) mm Tris-HCl (ph 8.) B) mm Tris-HCl (ph 8.) containing. M NaCl 7-8%B (- min), 8%B (- min) Flow rate :. ml/min Temperature : C Detection : UV at 6 nm Injection : L Plasmid pbr Hae digest (8-87 bp) Plasmid pbr (,6 bp) P867A min Eluent :. M KHPO-KHPO (ph 7.) containing. M NaCl Flow rate :.7 ml/min Temperature : ambient ( C) Detection : UV at 6 nm Injection : L '-CCGCTCGAGCTAAAAAAAGCCTGTGTTACC-' ( mer) mer F6C- min UV TIC Eluent : A) mm DBAA* (ph 6.) B) mm DBAA* (ph 6.)/acetonitrile (/) 8-6%B (- min), 6%B (- min) Flow rate :. ml/min Temperature : C Detection : UV at 69 nm and ESI negative-mode Injection : L ( pmol/component) * di-n-butylammonium acetate YMC Bioseparation Products

7

8 Ion Exchange Chromatography YMC-BioPro series AU.. N() N() N() Rs(,) Rs(,) MPa. N7K min AU... N7J N() N() N() Rs(,) Rs(,) min MPa. Ribonuclease A (. mg/ml). Cytochrome c (. mg/ml). Lysozyme (. mg/ml) Eluent Flow rate Temperature Detection Injection : A) mm KHPO-KHPO (ph 6.8) B) mm KHPO-KHPO (ph 6.8) containing. M NaCl - %B (- min) for X mmi.d. - %B (-7 min) for X mmi.d. :. ml/min : C : UV at nm : L 7 YMC Bioseparation Products

9 AU. 6 min AU. 6 min AU. 6 min IgG Transferrin N76E N78F N78D Albumin N8E min. Ribonuclease A (. mg/ml). Cytochrome c (. mg/ml). Lysozyme (. mg/ml) Eluent : A) mm KHPO-KHPO (ph 6.8) B) mm KHPO-KHPO (ph 6.8) containing. M NaCl -%B (-6 min) Flow rate :. ml/min for mmi.d..9 ml/min for. mmi.d. Temperature: C Detection : UV at nm Injection : L for mmi.d..6 L for. mmi.d. peaks = N8B 6 min N8A min IgG Transferrin IgG Transferrin Albumin Albumin peaks = 8 N87C 6 min peaks = N8G min Eluent Flow rate Temperature Detection Injection Sample : A) mm Tris-HCl (ph 8.6) B) mm Tris-HCl (ph 8.6) containing. M NaCl -%B (- min), -%B (- min) :. ml/min : C : UV at 8 nm : L : Human serum ( L/mL) N87G 6 min Eluent Flow rate Temperature Detection Injection Sample : A) mm Tris-HCl (ph 8.6) B) mm Tris-HCl (ph 8.6) containing. M NaCl -%B (- min), -6%B (-6 min) :. ml/min : C : UV at nm : L : Tryptic digest of BSA 8

10 Ion Exchange Chromatography YMC-BioPro series AU.. Column : YMC-BioPro QA X mmi.d. A (porous Q type) X mmi.d. B (porous Q type) X. mmi.d. Linear velocity :. cm/min Sample : mg/ml Bovine serum albumin (BSA) in equilibration buffer Equilibration buffer : mm Tris-HCl (ph 8.6) Elution buffer : mm Tris-HCl (ph 8.6) containing. M NaCl Detection : UV at 8 nm BioPro QA % breakthrough Ovalbumin. Trypsin inhibitor N86B N87C N87B N86E N88C N88B N86A N86D min min Bioseparation YMC Products.... Eluent : A) mm Tris-HCl (ph 8.) B) mm Tris-HCl (ph 8.) containing. M NaCl -8%B (- min) Flow rate :. ml/min Temperature : C Detection : UV at 8 nm Injection : L 9

11 AU... AU. N7K N76E 6 min P8A N8G N8B. ml/min.8-. MPa min. ml/min.- MPa. Ribonuclease A. Cytochrome c. Lysozyme Eluent Temperature Detection Injection min P8B NaN NaN MAb MAb min : A) mm KHPO-KHPO (ph 6.8) B) mm KHPO-KHPO (ph 6.8) containing. M NaCl - %B (- min) for YMC-BioPro SP-F -%B (-6 min) for YMC-BioPro SP : C : UV at nm : L X mmi.d. min X mmi.d. Eluent : A) mm Tris-HCl (ph 8.) B) mm Tris-HCl (ph 8.) containing. M NaCl -%B (-6 min) Flow rate :. ml/min Temperature : C Detection : UV at nm Injection : L (. mg/ml) Sample : Mouse monoclonal IgG anti-human IgG (Purified by DEAE chromatography, containing NaN) min Eluent : A) mm Tris-HCl (ph 8.) containing.7 M NaCl B) mm Tris-HCl (ph 8.) containing. M NaCl : -%B(- min) Flow rate :. ml/min Temperature : C Detection : UV at 6 nm Injection : L (. mg/ml)

12 Size Exclusion Chromatography YMC-Pack Diol MW 6 Diol- 6 Diol Diol-. IgM. Thyroglobulin. IgA. Fibrinogen. -Globulin 6. IgG 7. Transferrin 8. HSA (human serum albumin) 9. -Antitrypsin. Ovalbumin. Carbonic anhydrase. Trypsin inhibitor. Myoglobin. -Lactalbumin. Ribonuclease A 6. Cytochrome c MW 9, 67, 9,, 8,,, 66,,,,, 7,,,7, Elution volume (ml) Column : YMC-Pack Diol X 8. mmi.d. Eluent :. M KHPO-KHPO (ph 7.) containing. M NaCl Flow rate :. ml/min Temperature : C Detection : UV at 8 nm. Glutamate dehydrogenase. Lactate dehydrogenase. Enolase. Adenylate kinase. Cytochrome c MW 9,, 67,,, Column : YMC-Pack Diol X 8. mmi.d. Eluent :. M KHPO-KHPOpH 7. containing. M NaCl Flow rate :.7 ml/min Temperature : ambient Detection : UV at 8 nm min min min YMC Bioseparation Products

13 N866A N866E N866M N867B N866F N866B N866C N866D N866K N866G N866H 6 min Eluent :. M KHPO-KHPO (ph 7.) containing. M NaCl Flow rate :. ml/min Temperature : ambient ( C) Detection : UV at 8 nm 6 P88A Albumin Transferrin IgG 6 min Eluent :. M KHPO-KHPO (ph 7.) Flow rate :.7 ml/min Temperature : ambient ( C) Detection : UV at nm Injection : L Sample : Mouse ascites fluid (6 times dilution with water) min. Insulin (Bovine). Neurotensin. Angiotensin. Glycine Column : YMC-Pack Diol-6 X 8. mmi.d. Eluent :. M KHPO-KHPO ph 7. containing. M NaCl/ acetonitrile7/ Flow rate :.7 ml/min Temperature : ambient Detection : UV at nm MW Myoglobin. Ribonuclease A. Cytochrome c. InsulinBovine). Insulin B chain 6. -Mating factor 7. Neurotensin 8. Angiotensin 9. CCK-Octapeptide. Bradykinin. Angiotensin. Oxytocin. Angiotensin. Met-Enkephalin. Leu-Enkephalin 6. Tetraglycine 7. Glycine MW 7,,7,,7,96,68,67,96,,6,, P-8. P-. P-. P- MW 8, 8,,7,8 MW Diol-6 Diol- Diol- 9 Diol- Elution volume (ml) MW. Pullulan (P-8) 8,. Pullulan (P-) 8,. Pullulan (P-) 86,. Pullulan (P-),. Pullulan (P-) 8, 6. Pullulan (P-),7 7. Pullulan (P-), 8. Pullulan (P-),8 9. Maltopentadecaose (G),8. Maltoundecaose (G),8. Maltoheptaose (G7),. Maltopentaose (G) 8. Maltotriose (G). Maltose (G). Glucose (G) 8 min Elution volume (ml) Column : YMC-Pack Diol, X 8. mmi.d. Eluent : water Flow rate :. ml/min Temperature : ambient Detection : RI

14 Reversed Phase Chromatography r 6 min 7 A8B 6 min. BAM-P. [D-Ala,Met ]-Enkephalinamide. -Endorphin. Met-Enkephalin. [D-Ala,Met ]-Enkephalin 6. -Endorphin 7. -Endorphin 7 AD,) 87),76) 7) 88),899),6) Eluent : A) water/tfa(/.) B) acetonitrile/tfa (/.) -%B (- min), %B (- min) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm YMC Bioseparation Products

15 8 6 PC=8 * NG PC= * ND 6 8 min min Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -%B (- min) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm Injection : L Sample : Tryptic digest of -Lactoglobulin B C8, nm C8, nm 6 76.D, 6 79.D C8, nm C, nm D min 7.D min C8, nm. Cytochrome c. Insulin (Bovine). Amyloid -protein. Lysozyme. -Lactalbumin 6. Myoglobin,),7),),),) 7,) Column : m, X mmi.d. Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -6%B (- min) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm 6 8.D min

16 Reversed Phase Chromatography r A) water/tfa (/.) B) acetonitrile/tfa (/.).D 97.D min min. BSA 66,). Conalbumin 77,). Lipoxidase 96,) Column : YMC-Pack C ( nm) m X mmi.d. Eluent : -%B (- min), %B (- min) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm A) water/tfa (/.) B) acetonitrile/-propanol/tfa (//.) C, nm. BSA 66,). Conalbumin 77,). Lipoxidase 96,) Column : m, X mmi.d. Eluent : A) water/tfa (/.) B) acetonitrile/-propanol/tfa (//.) -%B (- min), %B (- min) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm 97.D 8 6 min C, nm C8, nm 7.D 78.D C, nm C8, nm 7.D 8.D 8 6 min 8 6 min YMC Bioseparation Products

17 S999A min Column : Hydrosphere C8 m X mmi.d. Eluent : mm KHPO-KHPO (ph 6.9) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm Injection : 8 L (.. mg/ml) min A9A Column : Hydrosphere C8 m X mmi.d. Eluent : mm KHPO-KHPO (ph.) Flow rate :. ml/min Temperature : C Detection : UV at 6 nm Injection : L (.. mg/ml) T T T J9A T T T T JA min T- T Impurities T mer Recovery 6% JE J9B min. 7. min Impurities mer min 7.. min Recovery % Column : m, X mmi.d. Eluent : A) mm or mm TEAA* (ph6.) B) mm or mm TEAA* (ph6.)/acetonitrile (8/) -6%B (- min) Flow rate :. ml/min Temperature : C Detection : UV at 69 nm * triethylammonium acetate '-CCGCTCGAGCTAAAA AAAGCCTGTGTTACC-' purity>99% Eluent : A) mm DBAA* (ph6.)/methanol (6/) B) mm DBAA* (ph6. )/methanol (/8) -%B (- min) Temperature : ambient Detection : UV at 69 nm Sample : synthetic oligonucleotide ( M) * di-n-butylammonium acetate 6

18 Reversed Phase Chromatography YMC-Pack PROTEIN-RP Eluent : water/tfa (/.) Flow rate :. ml/min Temperature : ambient Column : X mmi.d. Eluent : acetonitrile/water (/6) Flow rate :. ml/min Temperature : C Detection : UV at nm. Uracil. Benzene. Naphthalene. Diphenyl min N() min N() Met-Enkephalin. Leu-Enkephalin. Oxytocin. Bradykinin. Angiotensin I 6. Ribonuclease A 7. -Mating factor 8. Insulin (Bovine) 9. Cytochrome c. Lysozyme. BSA. -Lactoglobulin. Ovalbumin 7) ),7),6),96),7),68),7),),) 66,) 8,),). Cytochrome c. BSA. Ferritin,) 66,),) Column : YMC-Pack PROTEIN-RP m X mmi.d. Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -9%B (-6 min) Flow rate :. ml/min Temperature : ambient min min Detection : UV at nm Column : YMC-Pack PROTEIN-RP m X mmi.d. Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -9%B (- min) Flow rate :. ml/min Temperature : ambient Detection : UV at 8 nm 7 YMC Bioseparation Products

19 Normal Phase Chromatography 6 min A99E, 6 8 min A997I. TRP-GLY. GLY-GLY-PHE. Angiotensin I. Substance P. Insulin (Bovine) 6) 79),96),8),7) Eluent : A) acetonitrile/tfa/tea (/./.) B) water/tfa/tea (/./.) -%B (- min), %B (- min) Flow rate :. ml/min Temperature : ambient Detection : UV at nm Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -%B (- min), %B (- min) Flow rate :. ml/min Temperature : ambient Detection : UV at nm OH Phenol OH OH Catechol OH HO Resorcinol HO OH OH Hydroquinone OH HO Pyrogallol OH HO OH Phloroglucinol Eluent : hexane/ethanol (8/) Flow rate :. ml/min Temperature : C Detection : UV at nm.. 6. min A6A min A6C 8

20 Normal Phase Chromatography YMC-Pack Polyamine 9 Column : YMC-Pack Polyamine II X mmi.d. Eluent : acetonitrile/water (/) Flow rate :. ml/min Temperature : C Detection : RI, -6 RIU/FS Injection : L (-7 mg/ml) min F68H 8 6 YMC Bioseparation Products 6 6 Raffinose Lactitol Palatinit Lactose Maltose Maltitol Inositol Palatinose Sucrose Galactose() Mannose() Arabitol Ribitol Xylitol % 8% 8% Glucose Mannitol Sorbitol Fructose Xylose Fucose Glycerol Column : YMC-Pack Polyamine II X mmi.d. Temperature : C Erythritol Rhamnose Sucralose

21 Packing Materials YMCGEL Eluent Temperature Detection Purity: 9.9% : A) water/tfa (/.) B) acetonitrile/tfa (/.) 7%B (- min), 7-%B (- min) : ambient : UV at nm min min min Sample : Insulin bovine / Insulin human = / (w/w) Pro C8 ODS-A ODS-AQ PolymerC8 C8 Load: mg Purity: 99.% ASS ASS6 AAS AAS AAS AAS6 AAS6 AAS6 AAS AAS AAS AAS AAS AASA AQS AQS AQS6 AQS6 AQS AQS PC99S OCS OCS OCS OCS6 OCS6 OCS6 OCS OCS OCS Ph PHS PHS6 PHS C BUS BUS BUS BUS6 BUS6 BUS6 BUS BUS BUS CN CNS CNS6 CNS YMCbasic BA99S Diol DLS DLS6 DLS6 PROTEIN-RP PR99S

22 QA SP QA-F SP-F Non-porous Non-porous QAAS-6WP SPAS-6WP QFS-6WP QFS-6WP QFS-6WP SFS-6WP SFS-6WP SFS-6WP,,,, 8,,, 8, Diol-6 Diol- Diol- Diol- 6 DL6S-6WT DL6S-8WT DL6S-8WT DL6S-WT DL6S-WT DLS-6WT DLS-8WT DLS-8WT DLS-WT DLS-WT DLS-6WT DLS-8WT DLS-8WT DLS-WT DLS-WT DLS-6WT DLS-8WT DLS-8WT DLS-WT DLS-WT 9,, 6,, 6, 9,, 6,, 6, 9,, 6,, 6, 9,, 6,, 6, S- (X)SS-HWT (X)SS-WT (X)SS-WT (X)SS-HPWT (X)SPWT (X)SS-PWT (X)SS-HWT (X)SS-WT (X)SS-LWT (X)SS-WT (X)SS-WT (X)SS-WT (X)SS-WT (X)SS-WT (X)SS-H6WT (X)S6WT (X)SS-L6WT (X)SS-6WT (X)S6WT (X)SS-6WT (X)SS-L6WT (X)SS-6WT (X)SS-WT (X)SS-WT (X)SS-PWT (X)SS-PWT (X)SS-HWT (X)SS-WT (X)SS-LWT (X)SS-WT (X)SS-WT (X)SS-WT (X)SS-WT (X)SS-LWT (X)SS-WT (X)SS-WT (X)SS-WT,, 6,,, 6,,,, 6,,,, 8, 8,,,, 8, 8,,, 6,, 6, 7,,,, 6,, 6,,,, 8, 6, (X)SS-6WT (X)SS-L6WT (X)SS-6WT (X)SS-6WT (X)SS-6WT (X)SS-6WT (X)SS-6WT (X)SS-WT (X)SS-WT (X)SS-WT (X)SS-WT (X)SS-WT,,, 8, 6, 6, 76, 96,, 8,, 8, S- 8 8 RS8S-HWT RS8S-WT RS8S-WT RS8S-HPWT RS8PWT RS8S-PWT RS8S-HWT RS8S-WT RS8S-LWT RS8S-WT RS8S-WT RS8S-WT RS8S-WT RS8S-WT RS8S-H6WT RS86WT RS8S-L6WT RS8S-6WT RS86WT RS8S-6WT RS8S-L6WT RS8S-6WT RS8S-WT RS8S-WT RS8S-PWT RS8S-PWT RS8S-HWT RS8S-WT RS8S-LWT RS8S-WT RS8S-WT RS8S-WT RS8S-WT RS8S-LWT RS8S-WT RS8S-WT RS8S-WT RS8S-6WT RS8S-L6WT RS8S-6WT RS8S-6WT RS8S-6WT RS8S-6WT RS8S-6WT RS8S-WT RS8S-WT RS8S-WT RS8S-WT RS8S-WT,, 6,,, 6,,,, 6,,,, 8, 8,,,, 8, 8,,, 6,, 6, 7,,,, 6,, 6,,,, 8, 6,,,, 8, 6, 6, 76, 96,, 8,, 8, Ordering Information YMC Bioseparation Products

23 S- AAS-HWT AAS-WT AAS-LWT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-L6WT AAS-6WT AA6WT AAS-6WT AAS-6WT AAS-WT AAS-WT AAS-PWT AAS-PWT AAS-LWT AAS-WT AAS-WT AAS-WT AAS-WT AAS-6WT AAS-L6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-6WT AAS-6WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-PWT AAS-PWT AAS-LWT AAS-WT AAS-WT AAS-L6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT 8,,,, 8,,,,,,,,, 6,, 6, 7,,, 6,, 6,,,,, 6, 6,,, 7,, 8,,,, 8,, 6, 8,, 8, 6,, 6, 7,,, 6,, 6, 6, 8, 9, 6, 6, 8, 9,,, 68,, 8, S- AQS-HWT AQS-WT AQS-LWT AQS-WT AQS-WT AQS-WT AQS-WT AQS-WT AQS-6WT AQ6WT AQS-6WT AQS-6WT AQS-WT AQS-WT AQS-PWT AQS-PWT AQS-LWT AQS-WT AQS-WT AQS-WT AQS-WT AQS-L6WT AQS-6WT AQS-6WT AQS-6WT AQS-6WT AQS-6WT AQS-6WT AQS-6WT AQS-6WT AQS-WT AQS-WT AQS-WT AQS-WT AQS-WT AQS-WT AQS-6WT AQS-6WT AQS-WT AQS-WT,, 6,,,,,,,,, 7, 6,, 6, 7,,, 6,,, 8,,,, 78,, 7, 8, 8, 96,,,, 8,, 6, 8,, 8, S- (XX)S-HWT (XX)S-WT (XX)S-LWT (XX)S-WT (XX)S-WT (XX)S-WT (XX)S-WT (XX)S-WT (XX)S-6WT (XX)6WT (XX)S-WT (XX)S-WT (XX)S-PWT (XX)S-PWT (XX)S-WT (XX)S-WT (XX)S-L6WT (XX)S-6WT (XX)S-6WT (XX)S-6WT (XX)S-6WT,, 6,,,,,,,, 6,, 6, 7,, 6, 8,,,, 78,

24 URL YMC F TEL.-FAX.- ISO 9 JQA-7 88K F TEL.687-FAX (XX)S-6WT (XX)S-6WT (XX)S-6WT (XX)S-6WT (XX)S-WT (XX)S-WT (XX)S-WT (XX)S-WT (XX)S-WT (XX)S-WT (ZZ)S-6WT (ZZ)S-6WT (ZZ)S-WT (ZZ)S-WT (YY)S-WT (YY)S-WT (YY)S-PWT (YY)S-PWT (YY)S-WT (YY)S-WT (YY)S-L6WT (YY)S-6WT (YY)S-6WT (YY)S-6WT (YY)S-6WT (YY)S-6WT (YY)S-6WT (YY)S-6WT (YY)S-6WT (YY)S-WT (YY)S-WT (YY)S-WT (YY)S-WT (YY)S-WT, 7, 8, 8, 96,,, 8,, 8, 6, 8,,, 6,, 6, 7,, 6,, 6, 6, 8, 9, 6, 6, 8, 9,,, 68,,, S- BA99S-HWT BA99S-WT BA99S-LWT BA99S-WT BA99S-WT BA99S-WT BA99S-WT BA99S-WT BA996WT BA99S-6WT BA996WT BA99S-WT BA99S-WT BA99S-6WT BA99S-6WT BA99S-6WT BA99S-6WT BA99S-6WT BA99S-6WT 8,, 8, 67, 78, 8, 6,, 8, 6,, 7, 7, 8, 6, 7, 78, 7, 86, PR99S-WT PR99S-WT PR99S-6WT PR99S-6WT PR99S-WT PR99S-WT PR99S-WT 8, 9, 6, 8,,,, S-6 PC99S6-LWT PC99S6-WT PC99S6-6WT PC99S6-6WT PC99S6-6WT PC99S6-6WT PC99S6-WT, 6,, 6, 6, 6, 8, 6 DN6S-6WT DN6S-6WT DN6S-6WT DN6S-WT DN6S-WT DNS-6WT DNS-6WT DNS-6WT DNS-6WT DNS-WT DNS-WT 6, 7, 9,,,, 6, 7, 9,,, PBS-6WT PBS-6WT PBS-6WT PBS-6WT PBS-WT PBS-WT, 6,, 8, 6,,

Waters Sigma-Aldrich 1.0 mg/ml LC Waters Alliance HPLC ACQUITY UPLC H-Class Bio 30 cm Alliance HPLC UV ACQUITY UPLC TUV mm nm XBridge Protein B

Waters Sigma-Aldrich 1.0 mg/ml LC Waters Alliance HPLC ACQUITY UPLC H-Class Bio 30 cm Alliance HPLC UV ACQUITY UPLC TUV mm nm XBridge Protein B HPLC 3. µm BEH Stephan Koza, Susan Serpa, Hua Yang, Edouard Bouvier, and Kenneth J. Fountain Waters Corporation, Milford, MA, USA HPLC 200 0 HPLC 2 2010 UPLC 200 SEC 1 UPLC SE-UPLC 2 µm BEH BEH 100% SE-UPLC

More information

Preparative chromatography

Preparative chromatography 1980 HPLC column System for production/purification Packing material Flow reaction Contract purification CONTENTS 2 3 4 00 8 01 9 BioStream YMC Pilot 12 02 HPLC13 HPLC LC-Forte/R14 HPLC K-Prep15 18 HPLC

More information

TaKaRa BCA Protein Assay Kit

TaKaRa BCA Protein Assay Kit 研究用 TaKaRa BCA Protein Assay Kit 説明書 v201307da TaKaRa BCA Protein Assay Kit は 高感度にタンパク質溶液の比色定量を行う試薬であり 界面活性剤によって可溶化されたタンパク質溶液の定量も可能です BCA によるタンパク質定量の原理は 2 段階の反応に基づいています 第 1 段階では タンパク質溶液中のペプチド結合によって キットに含まれる二価銅イオン

More information

<90CE90EC88E290D55F955C8E862E656336>

<90CE90EC88E290D55F955C8E862E656336> 5 5 9 9 7 7 5 5 6 6 7 7 8 8 9 9 8 8 8 8 79 79 78 78 76 76 77 77 7 7 6 7 7 5 68 68 67 67 66 66 65 65 6 6 6 6 6 6 6 6 6 6 59 59 58 58 57 57 56 56 55 55 5 5 8 8 5 5 9 9 9 8 7 9 9 8 8 7 7 6 6 5 5 5 5 69 69

More information

a b Chroma Graphein Chromatography

a b Chroma Graphein Chromatography a b Chroma Graphein Chromatography (Stationary Phase) (Mobile Phase) CHROMATOGRAPHY GAS SFC LIQUID GSC GLC Column Planar NP RP IEC SEC TLC Paper Normal Phase Reverse Phase GPC GFC Thin Layer Chromato.

More information

2

2 1 2 3 4 5 (written by Dr. ) Stock Solution (one liter) in 4 Buffer A: 0.05 M glucose 20% (1.1 M) glucose 22.7 ml 0.025 M Tris-Cl ph8 1 M Tris (ph8.0) 12.5 ml 0.01 M EDTA 0.5 M EDTA 10 ml Buffer B: 5 M

More information

案内(最終2).indd

案内(最終2).indd 1 2 3 4 5 6 7 8 9 Y01a K01a Q01a T01a N01a S01a Y02b - Y04b K02a Q02a T02a N02a S02a Y05b - Y07b K03a Q03a T03a N03a S03a A01r Y10a Y11a K04a K05a Q04a Q05a T04b - T06b T08a N04a N05a S04a S05a Y12b -

More information

A4冊子1P-P20.ai

A4冊子1P-P20.ai REVERSE PHASE CLUMN GUIDE 1 2 Luna 5 μm Phenyl-Hexyl, 150x4.6 mm Luna 5 μm C8(2), 150x4.6 mm Luna 5 μm C18(2), 150x4.6 mm : 50:15:35 Methanol:Acetonitrile: 20mM Ammonium Formate ph 3.5 : 1.0 ml/min : 1.

More information

PAXgene Tissue DNA Kitプロトコールとトラブルシューティング(2301605 06/2009)

PAXgene Tissue DNA Kitプロトコールとトラブルシューティング(2301605 06/2009) January 2009 PAXgene Tissue DNA Kit PAXgene Tissue Containers DNA PAXgene Tissue Container PAXgene DNA 3 PAXgene DNA 6 PAXgene DNA 9 13 2 PAXgene Tissue DNA Kit 01/2009 PAXgene DNA DNA PAXgene Tissue Containers

More information

untitled

untitled 5 28 EAR CCLECCN ECCN 1. 2. 3. 4. 5.EAR page 1 of 28 WWW.Agilent.co.jp -> Q&A ECCN 10020A 10070A 10070B 10070C 10071A 10071B 10072A 10073A 10073B 10073C 10074A 10074B 10074C 10076A 10229A 10240B 10430A

More information

mastro_nakamen三校.ai

mastro_nakamen三校.ai Hydrocortisone Sodium Phosphate 3.5 1.0 0.5 1 μg/ml 3.0 2.5 25 ng/ml 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 min 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 min Fig.1 Chromatograms of Hydrocortisone

More information

ap0 ap1 ap2 ap3 ap4 ap5 ap6 ap7 ap8 ap9 aq0 aq1 aq2 aq3 aq4 aq5 aq6 aq7 aq8 aq9 aw0 aw1 aw2 aw3 aw4 aw5 aw6 aw7 aw8 aw9 ae0 ae1 ae2 ae3 ae4 ae5 ae6 ae7 ae8 ae9 ar0 ar1 ar2 ar3 ar4 ar5 ar6 ar7 ar8 ar9 at0

More information

DiovNT

DiovNT Peptide Antisera and Monoclonal Antibodies (2) Antisera of Yanaihara Institute Inc. Monoclonal Antibodies Peptide Antisera and Antisera of Yanaihara Institute Inc. 274 Manufacturer: YANAIHARA INSTITUTE

More information

16 2

16 2 15 16 3 16 2 2001 9 2003 6 10a3 3 2001 9 13 1 2 11 2 12g/100g.f.w 22g/100g.f.w 1g 30ml,0.1N NaOH2ml 10 3 30 0.1N HCl2ml F-kit ph3 F-kit 0.9 GFC-HPLC 4 HPLC YMC Pack Diol 200500 8.0mmID YMC Pack Diol 300300

More information

76 3 B m n AB P m n AP : PB = m : n A P B P AB m : n m < n n AB Q Q m A B AQ : QB = m : n (m n) m > n m n Q AB m : n A B Q P AB Q AB 3. 3 A(1) B(3) C(

76 3 B m n AB P m n AP : PB = m : n A P B P AB m : n m < n n AB Q Q m A B AQ : QB = m : n (m n) m > n m n Q AB m : n A B Q P AB Q AB 3. 3 A(1) B(3) C( 3 3.1 3.1.1 1 1 A P a 1 a P a P P(a) a P(a) a P(a) a a 0 a = a a < 0 a = a a < b a > b A a b a B b B b a b A a 3.1 A() B(5) AB = 5 = 3 A(3) B(1) AB = 3 1 = A(a) B(b) AB AB = b a 3.1 (1) A(6) B(1) () A(

More information

I

I I II 1 2 3 4 5 6 7 8 9 10 11 Rp.1) 50mg 3 3 14 Rp.2) 2.5mg 2 14 Rp.1) 2.5mg 3 2 2:1 14 Rp.) 15g 1 3 28 Rp.2) 50 600mg 1 2 28 12 Rp.1) CR 20mg 1 1 1 14 Rp.2) R 200 1 2 14 Rp.3) 3 1 3 14 Rp.1) 2.5mg 0.4

More information

2

2 th 37 ICh Theoretical Examination - - - - 5 - - : - ( ): ( ) - : 279 - - - - - - - G D L U C K 1 2 1 amu = 1.6605 10-27 kg N = 6.02 10 23 mol -1 k = 1.3806503 10-23 J K -1 e = 1.6022 10-19 C F = 9.6485

More information

05秋案内.indd

05秋案内.indd 1 2 3 4 5 6 7 R01a U01a Q01a L01a M01b - M03b Y01a R02a U02a Q02a L02a M04b - M06b Y02a R03a U03a Q03a L03a M08a Y03a R04a U04a Q04a L04a M09a Y04a A01a L05b, L07b, R05a U05a Q05a M10a Y05b - Y07b L08b

More information

Microsoft Word - 14_LCMS_アクリルアミド

Microsoft Word - 14_LCMS_アクリルアミド 2,4- (2,4-Dichlorophenoxy) acetic acid 2,4-D 2,4-PA OH O O C A S 94-75-7 C 8 H 6 Cl 2 O 3 Cl Cl ( ) 221. (219.9694) 1) (g/cm 3 ) (25 C) 2) ( C) (.4 mm Hg) 2) ( C) 2) (mmhg) (2 C) 2) (mg/l) (25 C) log P

More information

QIAquick Spin プロトコールとトラブルシューティング ( /2008)

QIAquick Spin プロトコールとトラブルシューティング ( /2008) March 2008 QIAquick Spin QIAquick PCR Purification Kit PCR 100 bp 10 kb QIAquick Nucleotide Removal Kit 17 40mer DNA 40 bp 10 kb QIAquick Gel Extraction Kit DNA 70 bp 10 kb Sample & Assay Technologies

More information

1 1 H Li Be Na M g B A l C S i N P O S F He N Cl A e K Ca S c T i V C Mn Fe Co Ni Cu Zn Ga Ge As Se B K Rb S Y Z Nb Mo Tc Ru Rh Pd Ag Cd In Sn Sb T e

1 1 H Li Be Na M g B A l C S i N P O S F He N Cl A e K Ca S c T i V C Mn Fe Co Ni Cu Zn Ga Ge As Se B K Rb S Y Z Nb Mo Tc Ru Rh Pd Ag Cd In Sn Sb T e No. 1 1 1 H Li Be Na M g B A l C S i N P O S F He N Cl A e K Ca S c T i V C Mn Fe Co Ni Cu Zn Ga Ge As Se B K Rb S Y Z Nb Mo Tc Ru Rh Pd Ag Cd In Sn Sb T e I X e Cs Ba F Ra Hf Ta W Re Os I Rf Db Sg Bh

More information

AVA卵巣癌適正使用改訂 indd

AVA卵巣癌適正使用改訂 indd Q&A P.58 CONTENTS 2 4 5 5 6 1. 6 2. 10 3. 12 4. 14 5. 15 6. 17 7. 19 8. 20 9. 22 10. 23 11. 24 12. 25 13. 26 14. 27 28 28 28 31 32 32 38 1 38 2 39 40 41 44 44 48 50 58 74 74 76 78 80 81 4 5 6 7 Q&A P.66

More information

2

2 1 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234

More information

1 2

1 2 ( ) ( ) ( ) 1 2 59 2 21 24 275 43 3 26 486 103 27 28 98 105 104 99 1 48 25 29 72 14 33 11-10 3 11 8 14,663 4 8 1 6.0 8 1 0.7 11-6 27 19 22 71 5 12 22 12 1,356 6 4,397 3 4 11 8 9 5 10 27 17 6 12 22 9

More information

VOL. 25 NO. 6 CHEMOTHERAPY 1667

VOL. 25 NO. 6 CHEMOTHERAPY 1667 1666 CHEMOTHERAPY AUG. 1977 VOL. 25 NO. 6 CHEMOTHERAPY 1667 1668 CHEMOTHERAPY AUG. 1977 VOL. 25 NO. 6 CHEMOTHERAPY 1669 VOL. 25 NO. 6 CHEMOTHERAPY 1671 1672 CHEMOTHERAPY AUG. 1977 VOL. 25 NO. 6 CHEMOTHERAPY

More information

48 13 13 13 38 21 13 3 13 3.1 l l l l l l 4.1.1 5mm 0.5mm 5mm 10 500mL 500mL 500mL 20 1 200 / 4 5 6 3000G 20 G1118 R N 2 10-8 GG Rcm Nrpm 1μm 2 3000G 1 /1 4.1.2PCB PCB 5mm 0.5mm 5mm 10 500mL 500mL 500mL

More information

O E ( ) A a A A(a) O ( ) (1) O O () 467

O E ( ) A a A A(a) O ( ) (1) O O () 467 1 1.0 16 1 ( 1 1 ) 1 466 1.1 1.1.1 4 O E ( ) A a A A(a) O ( ) (1) O O () 467 ( ) A(a) O A 0 a x ( ) A(3), B( ), C 1, D( 5) DB C A x 5 4 3 1 0 1 3 4 5 16 A(1), B( 3) A(a) B(b) d ( ) A(a) B(b) d AB d = d(a,

More information

閨 [

閨 [ 1303000709 000 03. 070503 170, 0 3 0806 タ07 09 090908090107060109 04030801 縺0408 縺0505 03010708030060405 タ05 縺0400703 060504050ィ 03090405080050400909 03.03. 030007030000908 060005090809 0501080507 080500705030504040701

More information

2 2 MATHEMATICS.PDF 200-2-0 3 2 (p n ), ( ) 7 3 4 6 5 20 6 GL 2 (Z) SL 2 (Z) 27 7 29 8 SL 2 (Z) 35 9 2 40 0 2 46 48 2 2 5 3 2 2 58 4 2 6 5 2 65 6 2 67 7 2 69 2 , a 0 + a + a 2 +... b b 2 b 3 () + b n a

More information

GPC/SEC... 3 GPC... 4 GPC... 5 Agilent PL-SP 260VS... 6 Agilent PL-GPC 220 GPC/SEC... 7 Agilent Cirrus GPC GPC... 9... 10 GPC/SEC... 11 GPC Agilent PL

GPC/SEC... 3 GPC... 4 GPC... 5 Agilent PL-SP 260VS... 6 Agilent PL-GPC 220 GPC/SEC... 7 Agilent Cirrus GPC GPC... 9... 10 GPC/SEC... 11 GPC Agilent PL GPC/SEC Greg Saunders Ben MacCreath GPC/SEC... 3 GPC... 4 GPC... 5 Agilent PL-SP 260VS... 6 Agilent PL-GPC 220 GPC/SEC... 7 Agilent Cirrus GPC GPC... 9... 10 GPC/SEC... 11 GPC Agilent PLgel Olexis... 12...

More information

20 57

20 57 56 20 57 58 59 12 60 ph 61 62 CCA 63 64 ( 1) 700 7.5 86% 54 17 71 2) 700 6.8 86% 50 15 65 3) 600 700 5.7 84% 36 14 50 550 650 4.2 80% 21 6 27 40 500 3.6 78% 16 7 23 3.6 78% 16 7 23 400 700 4.0 78% 18 7

More information

外因性内分泌攪乱化学物質調査暫定マニュアル(水質、底質、水生生物)

外因性内分泌攪乱化学物質調査暫定マニュアル(水質、底質、水生生物) N- 1) 2) 1) GC/MS HPLC GC/MS 2) 0.01~0.05µg/l 0.5~50µg/kg GC/MS SIM GC/MS SIM GC/MS SIM a) b) -d 10 -d 10 -d 12 -d 12 c) d) 600 C 4 e) 1 99.999% f) a) b) - IX-1 - c) (ODS) 200-1,000mg 5ml 5ml 5ml d) e)

More information

F8302D_1目次_160527.doc

F8302D_1目次_160527.doc N D F 830D.. 3. 4. 4. 4.. 4.. 4..3 4..4 4..5 4..6 3 4..7 3 4..8 3 4..9 3 4..0 3 4. 3 4.. 3 4.. 3 4.3 3 4.4 3 5. 3 5. 3 5. 3 5.3 3 5.4 3 5.5 4 6. 4 7. 4 7. 4 7. 4 8. 4 3. 3. 3. 3. 4.3 7.4 0 3. 3 3. 3 3.

More information

untitled

untitled I RITE A B C D E F A B C LSI D LSI E/ F / G H I J K/ L / M A B 1 Intelligent Thermal Technology 2 17 18 19 LSI / LSI / / / 3 A IT 105-130 180 IT 4 off 5 6 B IT A RECO-View 630BF 100 7 100 100 RD919 A 8

More information

1 WSV SIR m/z Analyte RT (Min) SIR m/z Cone voltage (V) Ascorbic Acid (C).91 177 2 Thiamine (B1) 1.1 265 5 Nicotinic Acid (B3) 1.27 124 15 Pyridoxal (

1 WSV SIR m/z Analyte RT (Min) SIR m/z Cone voltage (V) Ascorbic Acid (C).91 177 2 Thiamine (B1) 1.1 265 5 Nicotinic Acid (B3) 1.27 124 15 Pyridoxal ( ACQUITY UPLC H-Class ACQUITY QDa Mark E. Benvenuti, Dimple Shah, and Jennifer A. Burgess Waters Corporation, Milford, MA, USA MS ACQUITY QDa UPLC HPLC UV ACQUITY QDa LC EC 1925/26 CFR 21 HPLC 1 LC-MS ACQUITY

More information

AllPrep DNA/RNA Micro プロトコールとトラブルシューティング ( /2008)

AllPrep DNA/RNA Micro プロトコールとトラブルシューティング ( /2008) July 2007 AllPrep DNA/RNA Micro DNA RNA 5 x 10 5 5 mg Sample & Assay Technologies DNA RNA 3 DNA RNA 11 DNA RNA 19 24 2 AllPrep DNA/RNA Micro 07/2007 DNA RNA RNA AllPrep DNA Spin Column DNA RNeasy MinElute

More information

21 1 1 1 2 2 5 7 9 11 13 13 14 18 18 20 28 28 29 31 31 34 35 35 36 37 37 38 39 40 56 66 74 89 99 - ------ ------ -------------- ---------------- 1 10 2-2 8 5 26 ( ) 15 3 4 19 62 2,000 26 26 5 3 30 1 13

More information

グリセオール注インタビューフォーム

グリセオール注インタビューフォーム 2006 8 3 873999 871319 IF 1998 9 FAX 1 200mL 300mL 500mL 20g 30g 50g 10g 15g 25g 1.8g 2.7g 4.5g 0.9W/V 200mL500mL 1979 3 13 2004 7 9 1979 5 15 2006 8 300mL 1979 3 13 2004 7 9 1982 11 1 IF 1 MR 63 IF 10

More information

13 0 1 1 4 11 4 12 5 13 6 2 10 21 10 22 14 3 20 31 20 32 25 33 28 4 31 41 32 42 34 43 38 5 41 51 41 52 43 53 54 6 57 61 57 62 60 70 0 Gauss a, b, c x, y f(x, y) = ax 2 + bxy + cy 2 = x y a b/2 b/2 c x

More information

ISO

ISO ISO ISO ISO(International Standardization Organization) 135 ISO CD(Committee Draft) DIS(Draft for International Standard) FDIS(Final DIS) ISO ISO23500 2004.01.13 ANSI/AAMI RD52 2004.04.13 6 2004.08.09

More information

取扱説明書[NE-202]

取扱説明書[NE-202] NE-202 13.3 m m 1 2 3 m 4 5 6 7 a a a 8 9 10 11 12 a a a a 13 14 15 16 17 2.4 FH 1/XX 4 18 19 20 21 22 23 24 25 26 27 1 2 3 4 5 6 m 7 h 8 r 9 a P b c d e f g h i j ud k l m n o 28 29 30 31 32 33 34 35

More information

J60335-1(3 ) 250 480 IEC 60065 IEC 60601 IEC 60745 IEC 60950 IEC 61029 2.1 2.2.1 2.2.2 2.2.3 2.2.4 2.2.5 2.2.6-1- J60335-1(3 ) 2.2.7 2.2.8 2.2.9 2.3.1 2.3.2 2.3.3 2.3.4 2.3.5 2.3.6 2.3.7 2.4.1 2.4.2 2.4.3

More information

NewBead_no33_1216-OK.indd

NewBead_no33_1216-OK.indd No.33 2011 January C O N T E N T S 5 12 1 2 3 4 90 *SF-1 *SF-60 *SF-308LP *SF-309L *SF-316L *FC-DP3 *YT-DP3 *-308 R *-309L R *L-62CF *L-80 192110 6,800 120 808-0023 2-4-1 093-761-4631 101-0044 2-5-15 03-3252-0591

More information

[PDF] パーソナルスペクトルモニター GeneQuant pro 取扱説明書

[PDF] パーソナルスペクトルモニター GeneQuant pro 取扱説明書 GeneQuant pro GE imagination at work 71-2236-32 ....3...4...4... 5...5...6...6...7...8 DNARNA... 8... 8... 9... 10... 11 595546562 nm... 11 UV280 nm... 12.... 13... 14... 14.... 15 DNARNAoligo... 16 Base

More information

A G A G A G 4 1 1 2 3 4 5 6 7 110119118 b A G C G 4 1 7 * * G A C b a HIKJ K J L f B c g 9 K c d g e 7 G 7 1 G 1 aa g g g c L M G L H G G 4 aa c c A a c CB B C A G f A G f G 9 8 1 2

More information

... 1... 2... 2... 3... 3... 4... 4... 5... 6 (a) 20... 6 (b) 10... 7 (c) 10... 8 (d) 24... 8 (e) 7... 9 (f) 7... 10... 10 (a) 12... 10 (b) 34... 11..

... 1... 2... 2... 3... 3... 4... 4... 5... 6 (a) 20... 6 (b) 10... 7 (c) 10... 8 (d) 24... 8 (e) 7... 9 (f) 7... 10... 10 (a) 12... 10 (b) 34... 11.. ... 1... 2... 2... 3... 3... 4... 4... 5... 6 (a) 20... 6 (b) 10... 7 (c) 10... 8 (d) 24... 8 (e) 7... 9 (f) 7... 10... 10 (a) 12... 10 (b) 34... 11... 13 (a) 7... 13 (b) 8... 14 (c) 19... 14... 16...

More information

Microsoft PowerPoint - MonoTowerカタログ_ 最終.ppt [互換モード]

Microsoft PowerPoint - MonoTowerカタログ_ 最終.ppt [互換モード] 次世代型シリカモノリスカートリッジカラム MonoTower TM C18 次世代型シリカモノリスカートリッジカラム MonoTower TM C18 MonoTower TM C18 モノリスカートリッジを連結することで高い理論段数を低圧力で実現 粒子充填型 ODS カラム (3 μm, 250 3.0 mm I.D.) 15.5 MPa 分離不充分 (500 3.0 mm I.D.) 14.5

More information

ChIP Reagents マニュアル

ChIP Reagents マニュアル ChIP Reagents ~ クロマチン免疫沈降 (ChIP) 法用ストック溶液セット ~ マニュアル ( 第 1 版 ) Code No. 318-07131 NIPPON GENE CO., LTD. 目次 Ⅰ 製品説明 1 Ⅱ セット内容 1 Ⅲ 保存 2 Ⅳ 使用上の注意 2 Ⅴ 使用例 2 < 本品以外に必要な試薬 機器など> 2 1) 磁気ビーズと一次抗体の反応 3 2)-1 細胞の調製

More information

ml ml ml mg ml ml mm mm mm mm mm mm mm mm mm mm mm ml mmhg mmhg ml Acetylcholine ml mg mm mm mm mm mm mm ope ++ Uribe Davis S H S S : : : : : : : : : G

More information

vol.31_H1-H4.ai

vol.31_H1-H4.ai http://www.jmdp.or.jp/ http://www.donorsnet.jp/ CONTENTS 29 8,715 Vol. 31 2 3 ac ad bc bd ab cd 4 Point! Point! Point! 5 Point! Point! 6 7 314 611 122 4 125 2 72 2 102 3 2 260 312 0 3 14 3 14 18 14 60

More information

0811jour (Page 1)

0811jour (Page 1) Information magazine CONTENTS Human Friendly Hospital T h i s i s o u r h o T O P I C S 2 s p i t a l! Information magazine 体 験 しました! 病 院 フェスタ REPORT 3 T h i s i s o u r h o T O P I C S 4 s p i t a l!

More information

SIRIUS_CS3*.indd

SIRIUS_CS3*.indd SIRIUS Innovations SIRIUS SIRIUS Answers for industry. SIRIUS SIRIUS S00 S0 SIRIUS SIRIUS ZX0-ORAZ-0AB0 7.5kW 6 S00 7 8 7.5kW 9 S00 0 8.5kW S0 8.5kW S0 5 6 7 IO-Link AS-InterfaceRT 8 8US 5 6 SIRIUS SIRIUS

More information

&

& & & & c & B & c & & aa c c a a nk a a & a aa aaa & A A A A & 7 & & c og og & c c k g a og ok c c & 7 7 n & aa & & & & & & & & & & & g & & a a & & 171 1 & 1 7 1 1 6 de 666 66 6 7 6 f 6 & & c & 1 1 1 1 1

More information

™…{,

™…{, 16:30-17:40 1-36 1-37 1-38 1-39 1-40 1-41 1-42 33 10:00-11:10 1-43 1-44 1-45 1-46 1-47 1-48 1-49 12:00-12:50 LS4 34 16:30-17:40 1-50 1-51 1-52 1-53 1-54 1-55 1-56 35 16:30-17:40 1-57 1-58 1-59 1-60 1-61

More information

untitled

untitled DC Fan 8mm sq. General Specifications San Ace 8 15mm thick (GA type), 15mm thick, 2mm thick (GA type) 2mm thick, 25mm thick (GA type), 25mm thick (S type) 25mm thick (GV type), 25mm thick 25mm thick (San

More information

C18①s

C18①s ニーズに合わせて 種類のラインナップ! スタンダード C8 適度な炭素量で回収率を重視 高容量 C8 より強い保持力を発揮 ハイフロー C8 粘性サンプル向け ラージポア C8 高分子向け 充てん剤の仕様 スタンダード C8 充てん剤の仕様 充てん剤粒子径孔径炭素量 スタンダード C8 0µm 0Å % 高容量 C8 0µm 0Å 7% ハイフロー C8 00µm 0Å 8% ラージポア C8 0µm

More information

untitled

untitled 28 20 1 31 4 2 25 1 IgG IgA IgM IgE IgM 2 60 80 70 60 60 40 20 80 35 45 kg 150cc 100 120cc 100cc 30 40cc 7,000g 500cc 120cc 120 5=600cc 600g 30 2,100cc 1,000cc 3 4 14 5 6 7 8 9 10 11 12 SIDS SIDS SIDS

More information

R257_2_History of evol.indd

R257_2_History of evol.indd R257 1 1500 200 300 1400 1500 1600 W. 1639 W. 1638 1700 J.1772 1713 1763 J.1765 J. 1776 H. 1797 1800 H. 1805 J.1848 J. 1855 1868 69 1877 1827 P. 1830 1838 N. 1876 1900 1920 35 ( )1938 1947 1953 1969 1979

More information

™·”õ/sec3_p63_84/fiü“eflÅ

™·”õ/sec3_p63_84/fiü“eflÅ Section3 64 1 65 Section 3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 Babara E. Ainsworth et al. Med. Sci. Sports. Exevc.1993 66 67 Section 3 1 10 9 8 1 2 3 4 7 6 5 6 7 5 1 2 3 4 68 69 Section 3 2 70 Section 3

More information

Tornado Series selection SW TiCN HSS Co FAX VL PM

Tornado Series selection SW TiCN HSS Co FAX VL PM Metal Band Saw Blades Tornado Series selection SW TiCN HSS Co FAX VL PM Selection Chart Selection Chart Solids 3 Selection Teeth 4 note 1) Structurals, Tubing H section steels Light gauge steels Tube Products

More information

[PDF] 安心して機器をお使いいただくために AKTA

[PDF] 安心して機器をお使いいただくために AKTA ! One Point 1 20% 1 1 2 2 3 20% 4 5 20ml ÄKTAexplorer 10S, ÄKTAexplorer 10XT, ÄKTApurifier 10, ÄKTApurifier UPC 10 2µm 1 ml/min 0.35 MPa 2M NaOH 18-1120-94 2 ÄKTAexplorer, ÄKTApurifier ÄKTAexplorer 100,

More information

untitled

untitled C-Class * * G H! i 100km/h COMAND i http://www.mercedes-benz.co.jp/ 3 4 13 19 29 59 241 283 355 4 13 16 13 15 17 13 77 78 79 21 22 21 107 107 107 108 108 256 361 256 186 187 AC 188 193 191 187 189 192

More information

1. 52

1. 52 51 1. 52 5 2. 1 2 54 4 55 5 1 56 2 57 . 1 1 58 2 1 59 2 4 60 61 62 6 64 4. 65 66 67 5 1 2 4 68 1 69 2 70 1 2 71 72 1 2 7 1 2 74 75 1 76 2 77 1 2 78 4 79 5 80 6. 1 81 2 82 8 84 85 86 87 7. 88 89 8. column

More information

土壌の観察・実験テキスト −土壌を調べよう!−

土壌の観察・実験テキスト −土壌を調べよう!− ( ) 2006 7 20 i 21 1962 1969 1987 1992 2005 65 1972 1977 1997 1977 1998 1982 1998 2002 2004 2005 SPP 1999 ii 1 g cm m 6378km ( ) 4.2, 4.3 5.1 7.1 8.1 4.1 7.3, 7.4 7.1 1 2 7.1 8.2 2 5 6 1, 2 2.3 4.2, 4.3

More information

1990 IMO 1990/1/15 1:00-4:00 1 N N N 1, N 1 N 2, N 2 N 3 N 3 2 x x + 52 = 3 x x , A, B, C 3,, A B, C 2,,,, 7, A, B, C

1990 IMO 1990/1/15 1:00-4:00 1 N N N 1, N 1 N 2, N 2 N 3 N 3 2 x x + 52 = 3 x x , A, B, C 3,, A B, C 2,,,, 7, A, B, C 0 9 (1990 1999 ) 10 (2000 ) 1900 1994 1995 1999 2 SAT ACT 1 1990 IMO 1990/1/15 1:00-4:00 1 N 1990 9 N N 1, N 1 N 2, N 2 N 3 N 3 2 x 2 + 25x + 52 = 3 x 2 + 25x + 80 3 2, 3 0 4 A, B, C 3,, A B, C 2,,,, 7,

More information

目 次 1 当 座 口 振 込 通 知 書 P1 ( 機 械 様 式 第 87 号 A) ( 平 成 25 年 1 月 診 療 (3 月 支 払 分 ) 以 降 ) 2 増 減 点 連 絡 書 P4 ( 機 械 様 式 第 20 号 の1) 3 返 戻 内 訳 書 P6 ( 機 械 様 式 第 20

目 次 1 当 座 口 振 込 通 知 書 P1 ( 機 械 様 式 第 87 号 A) ( 平 成 25 年 1 月 診 療 (3 月 支 払 分 ) 以 降 ) 2 増 減 点 連 絡 書 P4 ( 機 械 様 式 第 20 号 の1) 3 返 戻 内 訳 書 P6 ( 機 械 様 式 第 20 増 減 点 連 絡 書 各 種 通 知 書 の 見 方 医 療 機 関 薬 局 平 成 25 年 4 月 社 会 保 険 診 療 報 酬 支 払 基 金 目 次 1 当 座 口 振 込 通 知 書 P1 ( 機 械 様 式 第 87 号 A) ( 平 成 25 年 1 月 診 療 (3 月 支 払 分 ) 以 降 ) 2 増 減 点 連 絡 書 P4 ( 機 械 様 式 第 20 号 の1) 3 返

More information

NL-06取扱説明書

NL-06取扱説明書 NL-06 NL-06 NL-06 3 NL-06 NL-06 i ii -10 +50 30 90%RH 3 iii ... 1... 3... 3... 4... 5... 8... 9... 10... 11... 11... 12 WS-02... 14... 14... 15 EC-04... 16 CP-11 CP-10... 18 LR-06 LR-07 LR-04... 20...

More information

2 3

2 3 Sample 2 3 4 5 6 7 8 9 3 18 24 32 34 40 45 55 63 70 77 82 96 118 121 123 131 143 149 158 167 173 187 192 204 217 224 231 17 285 290 292 1 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38

More information

JAJP.qxp

JAJP.qxp Agilent 1200 LC Our measure is your success. products applications software services - 4 90,000-20 HPLC - UV-Vis ELSD LC/MS - - Agilent 1200 LC (RRLC) 1 RRLC Agilent 1200 LC 2 2 ZORBAX HT 1.8 µm Poroshell

More information

<93C18F578B4C8E965F90F596EE20918F91BC2E6D6364>

<93C18F578B4C8E965F90F596EE20918F91BC2E6D6364> Laser Flash Exposure time 25(50)µsec Deadtime=150nsec 5µsec I0 I1 I2 I3 I4 I5 Camera (40000 or 20000 fps) Phosphorescence ɡ ɡ ɡ ɡ TSParticle Intensity Ratio [ ] 0.9 1 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 Fitted

More information

A B A E

A B A E 10533-68-3955 10533-68-3955 10533-68-3804 RP A-6 10533-68-3804 10533-69-9615 10533-57-2161 B-2 10533-68-2274 10533-68-2221 10533-67-6282 A-6 10533-57-2161 E-3 10533-68-5161 10533-68-3553 D-2 D-2 10533-69-5258

More information

Product Description The ability to isolate and study a purified protein lies at the heart of modern biochemistry. Researchers in many fields require h

Product Description The ability to isolate and study a purified protein lies at the heart of modern biochemistry. Researchers in many fields require h Printed August 09, 2011 Version 1.2 For Research Use Only. Not for use in diagnostic procedures. His tagged Protein PURIFICATION GEL (MoAb. clone 2D8) CODE No. 3311 / 3312 PURIFICATION to Maintains Protein

More information

06.indd

06.indd CO 2 1 2 6kW MAX120rpm 500Nm MAX 50kN MAX 150rpm 80Nm 3 1 20µm 1µm 2 35 45 W 3000mm D 9000mm 25t 35 35 3 FFT D2200mm W2800mm H1500mm 120rpm200N 0.72 400N m 60mm 300mm 300mm 4 ( ) 5 EPS EPS EPS EPS EPS

More information

CAT. No. 1102q 2014 G-2

CAT. No. 1102q 2014 G-2 A7 A4 4 45 46 75 76 83 84 09 0 8 82 205 206 243 244 279 280 303 304 325 326 333 334 345 346 355 356 379 33 AT. No. 02q NSKAT. No. 02q NSK SI JIS NSK NSK http://www.nsk.com A 7. A 7.2 A 7 A6 A8 3. A8 3.2

More information