steponeplus_bro_f-1124.indd
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1 PCR StepOnePlus /StepOne PCR
2 PCR PCR StepOnePlus StepOne PCR PCR
3 Features At A Glance StepOne StepOnePlus / FAM /SYBR Green dyes VIC /JOE dyes ROX dyes 1. NED /TAMRA dyes VeriFlex Block StepOnePlus StepOne PCR 48 StepOne 96 StepOnePlus 1 PCR 1 PCR PCR logs 30 L 10 RNase P StepOne StepOne 96 StepOne StepOnePlus PCR StepOne StepOnePlus 3 48StepOne 4 96 StepOnePlus PCR LED FAM SYBR Green VIC JOE ROX SNP / StepOnePlus TAMRA 40 Fast PCR LCD USB E StepOnePlus VeriFlex PCR 6 StepOne StepOnePlus
4 StepOnePlus StepOne USB LAN Local Area Network E E StepOnePlus StepOne 5 LAN LAN LAN 4. StepOne 5
5 StepOnePlus StepOne PCR 5 4 CT PCR SNP E & PowerPoint Excel jpeg 5. StepOne Multiple Plots 1 4 Multiple Plots SNP 6. StepOne PCR StepOne LAN
6 StepOnePlus StepOne FAM VIC TaqMan MGB VIC TAMRA SYBR Green I StepOnePlus TAMRA TaqMan MGB SYBR Green I 1 StepOnePlus StepOne CT CT StepOnePlus StepOne SNP microrna StepOnePlus StepOne Fast 2 Fast 40 PCR Fast 10 Amplification Plot Allele Discrimination Plot (SNP Assay: SNP Assay 1) ΔRn 1 Allele Cycle Allele 1 Homozygous 1/1 Heterozygous 1/2 Homozygous 2/2 Undeterminded 7. PCR StepOne 10 18S rrna TaqMan 9 log 8. SNP CYP2C19*2 TaqMan SNP Genotyping Assays StepOnePlus
7 VeriFlex 96 StepOnePlus PCR 6 VeriFlex VeriFlex PCR PCR VeriFlex PCR 6 VeriFlex VeriFlex (96 ) PCR VeriFlex StepOnePlus StepOne PCR StepOnePlus StepOne 5,000 10,000 DNA 99.7 StepOnePlus StepOne 79log 3.4 Melt Curve 10 Multiplex Assays 2.9 Derivative Reporter (-Rn ) ΔRn Tm: 79.2 Temperature ( C) Cycle 9. PCR 18S GAPDH 3 Tm 10. StepOnePlus TaqMan 18S rrna VIC TGF- FAM cdna 48
8 2 < depth: 48.5 cm < depth: 48.5 cm < height: 51.8 cm > > < height: 51.8 cm > > < > width: 24.6 cm < > width: 24.6 cm StepOne 48 3 StepOnePlus StepOnePlus 96 4 VeriFlex
9 PCR / StepOnePlus StepOne PCR PCR StepOnePlus StepOne PCR 1 StepOnePlus StepOne StepOne StepOnePlus Ambion Applied Biosystems RNA Ambion Applied Biosystems RNA Ambion RNA TaqMan MicroRNA Assays microrna TaqMan TaqMan MicroRNA Assays MicroRNA Human Mouse Rat Arabidopsis Drosophila C. elegans mirna.appliedbiosystems.com TaqMan SNP Genotyping Assays TaqMan SNP Genotyping Assays HapMap SNPs 70,000 csnps 160,000 2,500 Drug Metabolism Genotyping Assays Custom TaqMan SNP Genotyping Assays SNP TaqMan SNP Genotyping Assays Primer Express Applied Biosystems Primer Express v3.0 TaqMan PCR SYBR Green I Primer Express StepOnePlus StepOne
10 TaqMan Gene Expression Assays 700,000 PCR TaqMan Gene Expression Assays TaqMan Assays PCR PCR Custom TaqMan Gene Expression Assays TaqMan Gene Expression Assays TaqMan Gene Expression, SNP Genotyping, and MicroRNA Assays TaqMan Assays Gene Expression* SNP Genotyping MicroRNA TaqMan Yes Yes Yes InventoriedMade-to-Order Custom TaqMan Assays Yes Yes No Human > 2,024,000 > 4,000,000 > 300 Mouse > 179,000 > 10,000 > 240 Rat > 128,000 > 180 Drosophila melanogaster > 38,000 > 50 Arabidopsis thaliana > 95,000 > 40 C. elegans > 90,000 > 60 Canine > 6,000 N/A Rhesus macaque > 70 N/A * mirna Gene copy number Mitochondrial HapMap drug metabolism genotyping SNP TaqMan 11.
11 TaqMan Fast Universal PCR Master Mix TaqMan Universal PCR Master Mix Power SYBR Green PCR Master Mix 96 StepOnePlus /StepOne PCR Material List 11 Applied Biosystems StepOnePlus /StepOne PCR MicroAmp 48-Well Optical Adhesive Film MicroAmp 48-Well Optical Adhesive Film MicroAmp 96-Well Optical Adhesive Film MicroAmp 96-Well Optical Adhesive Film MicroAmp Fast Optical 48-Well Reaction Plate MicroAmp Fast Optical 96-Well Reaction Plate with Barcode (0.1 ml) MicroAmp Fast Optical 96-Well Reaction Plate with Barcode (0.1 ml) MicroAmp Fast 8-Tube Strip, 0.1 ml MicroAmp Optical 8-Cap Strip MicroAmp Fast 48-Well Tray MicroAmp 96-Well Tray for Veriflex Blocks MicroAmp 48-Well Base Adaptor Power SYBR Green PCR Master Mix 5mL TaqMan Gene Expression Master Mix 5mL TaqMan Genotyping Master Mix 10mL TaqMan Fast Universal PCR Master Mix TaqMan One-Step RT-PCR Master Mix Reagents Kit High Capacity cdna Reverse Transcription Kit
12 Ordering Information StepOne PCR StepOnePlus PCR StepOnePlus PCR * StepOne-01 StepOnePlus-01 *StepOne PCR PCR NOTICE TO PURCHASER: The StepOne [and StepOnePlus ] Real-Time PCR System is [Systems are ] covered by one or more of US Patents Nos. 5,038,852, 5,333,675, 5,656,493, 5,475,610, 5,602,756, 6,703,236, 7,238,517, 6,814,934, and corresponding claims in their non-us counterparts, owned by Applera Corporation. No right is conveyed expressly, by implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5' nuclease methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. Applied Biosystems, AB (Design), Primer Express and VIC are registered trademarks and Applera, FAM, JOE, MicroAmp, NED, StepOne, StepOnePlus, TAMRA and VeriFlex are trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. TaqMan is a registered trademark of Roche Molecular Systems, Inc. SYBR is a registered trademark of Molecular Probes, Inc. 2011, Life Technologies Japan Ltd. All rights reserved. Printed in Japan, 12/2011, SDS024-B1112OB TEL FAX TEL FAX
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StepOnePlus /StepOne PCR PCR PCR StepOnePlus StepOne PCR PCR Features At A Glance StepOne StepOnePlus / 48 96 FAM /SYBR Green dyes VIC /JOE dyes ROX dyes 1. NED /TAMRA dyes VeriFlex Block StepOnePlus StepOne
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StepOnePlus / StepOne PCR PCR Applied Biosystems StepOnePlus StepOne PCR 1 Features At A Glance StepOne StepOnePlus / 48 96 FAM/SYBR Green dyes VIC/JOE dyes ROX dyes NED/TAMRA dyes 1. VeriFlex Block StepOnePlus
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SUBJECT: Applied Biosystems Data Collection Software v2.0 v3.0 Windows 2000 OS : 30 45 Cancel Data Collection - Applied Biosystems Sequencing Analysis Software v5.2 - Applied Biosystems SeqScape Software
TaqMan MGB 遺伝子発現検出キットのご注文について 注文書の記入方法 2 ページ目以降が注文書になっています 注文書に日付とご氏名 ご所属 所在地 電話番号 FAX 番号をご記入下さい * 注文書が複数になる場合は 記入したものをコピーしてご使用下さい 注文するプローブについての情報をご記入
TaqMan MGB 遺伝子発現検出のご注文について 注文書の記入方法 2 ページ目以降が注文書になっています 注文書にとご氏名 FAX 番号をご記入下さい * 注文書が複数になる場合は 記入したものをコピーしてご使用下さい 注文するプローブについての情報をご記入下さい : 下の表で確認し にチェック : 英字または数字 15 ケタ以内で記入 リポーター色素 : ご希望の仕様を にチェック注文本数の合計
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TaqMan MGB 遺伝子発現検出のご注文について 注文書の記入方法 2 ページ目が注文書になっています 注文書にと FAX 番号をご記入下さい * 注文書が複数になる場合は 記入したものをコピーしてご使用下さい 注文するプローブについての情報をご記入下さい : 下の表で確認し にチェック : 英字または数字 15ケタ以内で記入リポーター色素 : ご希望の仕様を にチェック注文本数の合計 : 注文書が複数になる場合は1
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