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1 Permeable Supports Selection Guide Transwell Falcon

2 Corning w In vivo w w w in vivo Create a More Natural Environment for Your Cells Costar ready-to-use 25 Falcon Corning BioCoat Falcon Corning BioCoat Corning BioCoat Falcon PET Falcon BioCoat Falcon 2

3 4 1 3 PC µm PET PET w Falcon PET w Falcon HD PET w Corning PET nm 99% PTFE PTFE µm µm µm µm HMVEC HUVEC , HUVEC HMVEC Jurkat Osteoblasts , , 5.0, , , , , , 5.0, , 3.0 Caco-2 MDCK , , 1.0 3

4 3 w mm w HTS w w 6 12 mm * cm mm 44 Falcon Corning FluoroBlok Corning BioCoat mm * cm /0.33** * ** 24 HTS mm HTS 4 ECM Corning BioCoat 7 BioCoat TEL ScientificSupportJP@corning.com 4

5 4 w PC 0.4 m 8.0 m w PET w Falcon PET PET mm w PET Corning FluoroBlok µm PET PC PET PC PC PC Falcon Corning BioCoat µm PET PET HD PET PET PET HD PET Falcon BioCoat Falcon Falcon 11 5

6 Corning FluoroBlok Corning nm PET Falcon Corning FluoroBlok 6.5 mm Corning FluoroBlok µm PET PET Corning FluoroBlok Corning FluoroBlok 24 Falcon 9 9 Snapwell 12mm Snapwell µm Snapwell PET PC Costar Costar Snapwell Netwell Netwell µm PET PET Netwell Costar Costar

7 ECM Corning BioCoat ECM Corning Caco-2 Corning Corning BioCoat BioCoat µm PET PET PET PET I I IV GFR * BioCoat µm PET PET PET BioCoat Falcon BioCoat ECM µm PTFE PTFE I & III 6 PTFE

8 HTS HTS Caco-2 HTS HTS µm PET PC PET PC PC PC HTS Transwell Falcon HTS µm PET PET PET PET PET HTS Falcon HTS HTS µm PAMPA PET PET I I Corning Gentest BioCoat BioCoat BioCoat BioCoat BioCoat Angiogenesis Angiogenesis a b C a / HUVEC VEGF 1 3 µm b in vitro HMVEC-1 NIH-3T3 1 Corning 3 µm c in vitro Corning 8 µm 8

9 mm cm 2 m / * 48 20, / * 48 20, / * 48 20, / * 48 20, / 48 27, / 48 27, / 24 13, / 24 13, / 24 13, / 12 46, / 12 46,400 * 6.5 mm / 24 36, / 24 36, / 48 31, / 48 31, / * 48 27, / * 48 27, / * 48 26,500 * 6.5 mm * mm cm 2 m PC , ,000 * Harvard Apparatus Corning FluoroBlok PET PET , , ,600 mm m / 48 27, / 48 27, / 48 34, / 48 34, , , mm mm , ,920 9

10 Falcon PET mm cm 2 m PET , PET , PET , PET , PET , PET , PET , PET , PET ,300 Falcon PET PET , PET , PET , PET , PET , PET , PET , PET , PET ,300 Corning BioCoat I PET , PET , * PET , PET , * PET , * PET ,700 Corning BioCoat IV * PET , * PET , PET ,700 Corning BioCoat * PET , PET ,700 Corning BioCoat * PET , PET , PET ,700 Corning BioCoat FluoroBlok * PET 24 32,700 Corning BioCoat Corning BioCoat PET , PET , * PET ,400 10

11 Corning BioCoat Caco-2 * BioCoat ml 1 Entero-STIM 400 ml 1 MITO 1 mm cm 2 m PET ,400 Corning BioCoat , , , ,460 Corning BioCoat * PET , * PET , * PET , * PET , * PET , PET , * PET , PET ,500 - mm cm 2 m , , , , , ,700 HTS HTS -24 mm cm 2 m PC , PC , PC , PC , , PET , PET ,000 Corning BioCoat HTS Caco-2 BioCoat ml 1 Entero-STIM 250 ml 1 MITO ml BioCoat HTS Caco-2 1 / PET 1 50, BioCoat HTS Caco-2 5 / PET 5 195,300 Corning BioCoat I 24 HTS * HTS PET 1 35,900 * BSE 11

12 Corning BioCoat I 24 mm cm 2 m PET ,400 Corning BioCoat PET , * PET , PET , * PET ,100 Corning BioCoat * * PET PET , ,000 Corning BioCoat * PET PET PET PET , , , ,100 Corning Gentest PMAPA PVDF ,500 Falcon PET , * PET , * PET , * PET ,200 Corning FluoroBlok * PET PET PET PET , , , ,700 Falcon , , /RS Tray** 60 35, Corning , , ,400 * ** RS Tray 12

13 HTS Falcon 96 mm cm 2 m PC , PC , PET , PET , PET , PC , PC , PC , PC , PET , PET , , , , , PET , PET , PET , , ,010 Corning FluoroBlok * PET PET PET PET , , , , ,300 * 13

14 Corning Life Sciences Protocols w Cell Migration, Chemotaxis and Invasion Assay Protocol CLS-AN-061 w Cell Migration, Chemotaxis and Invasion Assay Using Staining Protocol CLS-AN-211 w Fixation and Staining Procedure for Transwell Inserts Protocol w Preparation of Transwell Inserts for Histology Protocol w Trypsinization Procedure for Transwell Inserts Protocol CLS-AN-033 References and Bibliographies w Testing Cell Monolayer Integrity on Transwell Permeable Supports CLS-AN-047W Selection and Use Guide w Transwell Permeable Supports Selection and Use Guide CLS-CC-007W Technical Reports w Compatible Fluorophores and Dyes for Corning FluoroBlok Inserts and Insert Systems CLS- DL-CC-077 w Considerations when Optimizing your Chemotaxis or Invasion Assay with Corning Transwell Permeable Supports CLS-AN-188 w Corning HTS Transwell-96 Permeable Support Protocols for Drug Transport Application Note CLS-AN-058 w Design and Evaluation of an Automation-Compatible Multiwell Insert for Cell-Based Assay CLS-DL-CC-072 w In Vitro Study of Cytokine-mediated Activation of Endothelial Cell Permeability Using Falcon Cell Culture Permeable Supports CLS-DL-CC-068 w Migration of Human Mesenchymal Stem Cells using Corning FluoroBlok Inserts CLS-DL- CC-054 w New PET Membrane for Corning FluoroBlok 3.0 m and 8.0 m Pore Size Cell Culture Inserts CLS-DL-CC-042 w Preparation of Falcon Cell Culture Permeable Supports for Confocal Indirect Immunofluorescence: Fixation and Staining of Caco-2/bbe C2 Cells with Various Dyes CLS-DL- CC-079 w Screening of Anti-Metastatic Compounds by a Fluorescence-Based Tumor Cell Invasion Assay CLS-DL-CC-076 w Use of Falcon Cell Culture Permeable Supports to Reconstruct a Differentiated Human Epidermis In Vitro CLS-DL-CC HTS Caco HTS Caco (Caco-2)

15

16 Beginning-to-end Solutions for Cell Culture At Corning, cells are in our culture. In our continuous efforts to improve efficiencies and develop new tools and technologies for life science researchers, we have scientists working in Corning R&D labs across the globe, doing what you do every day. From seeding starter cultures to expanding cells for assays, our technical experts understand your challenges and your increased need for more reliable cells and cellular material. It is this expertise, plus a 160-year history of Corning innovation and manufactur ing excellence, that puts us in a unique position to offer a beginning-to-end portfolio of high-quality, reliable cell culture consumables For a listing of trademarks, visit us at All other trademarks are the property of their respective owners Tel Fax CLSJP@corning.com Tel: ScientificSupportJP@corning.com 2016 Corning Incorporated CLS R T

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