Preparative chromatography

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2 1980 HPLC column System for production/purification Packing material Flow reaction Contract purification CONTENTS BioStream YMC Pilot HPLC13 HPLC LC-Forte/R14 HPLC K-Prep15 18 HPLC K19 YMC-Actus20 SB GMPFAT, IQ/OQ, CSV

3 YMC BioStream HPLC K-Prep SB DAU YMCGELFDA,DMF YMC Pro CHIRAL ART YMC-Triart YMC-Actus YMC-BioPro YMC Pilot 1980 YMC-Pack YMCGEL 3

4 HPLC HPLC HPLC LC-Forte/R K-Prep LAB K-Prep FC LC-Forte/R LC-Forte/R0 LAB0S LAB0G LAB300S LAB300G FC750S FC750G FC1500S FC1500G (ml/min) (MPa) ml/min ml/min 15 (W D H) mm mm mm LC-Forte/R Forte Separa K-Prep GMP, CSV YMC-Actus SB DAD / DAU DAD S DAU S DAD DAU-0-700S mm 20, 30 50, 70, 0, 150, 200, , 0 mm 50, 75, 0, 150, , (MPa)

5 HPLC K-Prep EX EX03KS EX03KG EX06KS EX06KG EX12KS EX12KG EX25KS EX25KG (ml/min) (MPa) (W D H) mm K-Prep GMP, CSV 17 DAD / DAU DAD DAU DAD DAU DAD DAU DAD DAU DAD DAU mm mm 700 (MPa) DAD DAU

6 HPLC HPLC K K-0 K-300 K-500 K-00 (ml/min) (MPa) (W D H) mm mm LCD HPLC K-Prep EX 60KG GMP, CSV K-PrepBioStream IQ/OQ 19 DAD / DAU 2700 ISO DAR mm 25 IQ/OQ 6

7 LPLC BioStream BSTP-800 BSTP-03K BSTS-03K BSTS-K BSTS-30K (ml/min) (MPa) (W D H) (0.6) mm mm mm mm BioStream GMP, CSV 3 YMC Pilot PI0/500 PI0/850 PI140/500 PI140/850 PI200/500 PI200/850 PI300/500 PI300/850 mm mm 500, , 850 (MPa)

8 LC-Forte/R LC-Forte/R YMC-DispoPackAT 30 mm ECO PLUS /ECO YMC-Actus K-Prep LAB 2050 mm ECO PLUS /ECO YMC-Actus K-Prep FC 5050 mm K-Prep EX 0 mm BioStream YMC Pilot ECO PLUS /ECO LC-Forte/R 30 mm ECO PLUS /ECO YMC-Actus K-Prep LAB K-Prep FC 2050 mm ECO PLUS /ECO YMC-Actus 5050 mm K-Prep EX 0 mm LC-Forte/R YMC-GPC 8

9 CONTENTS BioStream YMC Pilot 12 9

10 BioStream LC cgmp CIP/SIP 21.5 IQ/OQCSV (ml/min) (MPa) ( C) BSTP-800 BSTP-03K PFAPTFEEPDM BSTS-03K 3000 BSTS-K 000 BSTS-30K (0.6) 5 30 SUS316LPTFEEPDM phuv3 BioStream (W D H) mm mm mm mm mm (kg) V (15 A) 200 V (20 A) 200 V (30 A) 0.5 MPa 200 V (40 A) MARK POD AV BPV FE PE EC PH UV TE As Ls AT IN DA F S C NAME Diaphragm Pump Air-operated 2way Diaphragm Valve Back Pressure Valve Flow Sensor Pressure Sensor Electric Conductivity Meter ph Meter UV Sensor Thermometer Air Switch Level Switch Air Trap Inlet Port Air Port Fraction Port Sample Port Column

11 BioStream 11

12 YMC Pilot IQ/OQFDA YMC Pilot mm mm L min max cm2 MPa PI0/500 PI0/ PI140/500 PI140/ PI200/500 PI200/ mm 12

13 HPLC CONTENTS HPLC LC-Forte/R 14 HPLC K-Prep HPLC K 19 YMC-Actus 20 SB

14 HPLC LC-Forte/R HPLC 3 LC-Forte/R LC-Forte/R0 LC-Forte/RHPLC (ml/min) (MPa) (W D H) LC-Forte/R mm LC-Forte/R18 UVRIRI PC LC-Forte/R ml/min ml/min 14

15 HPLC K-Prep LAB PC K-Prep LAB0 K-Prep LAB300 K-Prep LABK-PrepHPLC LAB0S LAB0G LAB300S LAB300G (ml/min) (MPa) (W D H) 5ch UV/VIS nm mm K-Prep 15

16 HPLC K-Prep FC PC IQ/OQCSV K-Prep FC750 K-Prep FC1500 K-Prep FCK-Prep HPLC FC750S FC750G FC1500S FC1500G (ml/min) (MPa) ch ml/min UV/VIS nm K-Prep AC0V0.5 MPa 16

17 HPLC K-Prep EX HPLC IQ/OQCSV K-Prep EXGMPHPLC MARK E F S P C 3PBV 3MBV FE MIX PE TE UV EC PH RV NAME Solvent Port Fraction Port Sample Port Pump Column Pneumatic Drive 3way Ball Valve Manual 3way Ball Valve Flow Sensor Mixer Pressure Sensor Thermometer UV/VIS Detector Electric Conductivity Meter PH Meter Relief Valve 17

18 K-PrepBioStream FDA 21 CFR Part11, cgmp, CSV K-Prep BioStream LC-Forte/R & PCForte Separa GPC LC-Forte/RLC-Forte/R 18

19 HPLC K KHPLC K-Prep (ml/min) (MPa) (W D H) K K K K mm mm 19

20 YMC-Actus YMC-Actus Axial Compression Technology μmnm C% ph Triart C ph 0 YMC-Triart Triart C18 ExRS Triart C C18 C18 C18 ph π-π Triart Phenyl Triart PFP ODS Pro C Hydrosphere C Pro Pro C18 RS C18 Pro C YMC-Pack ODS-A ODS-AQ ODS 20

21 SB SB SB-50 SB-70 SB-0 SB-150 SB-200 mm MPa mm / mm mm /

22 DAD IQ/OQ mm (MPa) (mm) (mm) DAD DAD-0 0 DAD DAD DAD DAD-450 DAD-600 DAD-800 DAD m 3.5 m 3.5 m 4.0 m 4.2 m 4.5 m

23 DAD HPLC 23

24 DAU IQ/OQ mm (MPa) (mm) (mm) DAU DAU-0 0 DAU DAU DAU DAU DAU m 2.5 m 2.8 m 3.0 m 3.5 m 4.0 m 4.0 m DAU

25 DAU 25

26 DAD 50DAU th plates/m plates/m Tf Tf 3rd 5th st 5 15 min Packing number analytical Scale up preparative 1.Cytchrome c MW Insulin MW Lysozyme MW α-Lactalbumin MW Myoglobin MW min Column : YMC-Pack C8 (5 μm, 20 nm) mmi.d. Flow rate : 0.8 ml/min 0 20 min Column : YMC*GEL C8 ( μm, 20 nm) mmi.d. Eluent : A) water / TFA (0/0.1) B) acetonitrile / TFA (0/0.1) 25-60%B (0-20 min) Flow rate : 0 ml/min Temperature: 37 C Detection : UV at 220 nm 26

27 CONTENTS GMP FATIQ/OQCSV

28 μm mm mg 0 mg g 0 g kg kg mml.d ,000 F = F (Dc /Dc)2 ml/min mg ,500 1, ,900,000 11,800 6,000 (6 L) 12,000 (12 L) 60,000 (60 g) 47,300 24,000 (24 L) 47,000 (47 L) 240,000 (240 g) F[mL/min] F [ml/min] Dc [mm] Dc [mm] 28

29 N(HETP) As N mm N = 5.54 x (tr / W0.5h)2 HETP HETP L / N L[m] As1 As b / a tr h W0.5h a b h/2 h/ trretention time hpeak height W0.5hBandwidth at half-height 29

30 GMP GMP FAT FAT IQ/OQ IQ OQ CSV CSV CSV FS OQ DS IQ 30

31 GMP 31

32 サポートネットワーク ワイエムシィグループの国内外拠点を通じて 全世界への製品供給体制とサービス体制を確保しています 今後も引き続き 製薬ビジネスの急成長が期待できる国や地域を中心に拠点の拡大を図ります Kyoto, Japan Ishikawa, Japan Tokyo, Japan YMC Europe GmbH Dinslaken, Germany YMC Engineering Co., Ltd. YMC America, Inc. Kyoto, Japan Allentown, U.S.A. YMC Shanghai Rep. Office Shanghai, China YMC Korea Co., Ltd. Gyeonggi-do, Korea 株式会社ワイエムシィ 京都本社 株式会社ワイエムシィ 京都研究所 株式会社ワイエムシィ 小松事業所 石川県 株式会社ワイエムシィ 東京営業所 株式会社YMCエンジニアリング 京都府 YMC America, Inc. アメリカ YMC Europe GmbH ドイツ YMC India Pvt. Ltd. インド YMC Shanghai Rep. Office 中国 YMC Korea Co., Ltd. 韓国 YMC Taiwan Co., Ltd. 台湾 YMC Singapore Tradelinks Pte. Ltd. シンガポール YMC India Pvt. Ltd. New Delhi, India YMC Taiwan Co., Ltd. Taoyuan, Taiwan YMC Singapore Tradelinks Pte. Ltd. Singapore 分取装置 分取カラムのご注文 お問い合わせは 専用ダイヤル 専用メールで 専門のスタッフがわかりやすくご説明いたします お気軽にお問い合わせください 受付 祝日を除く月 金曜日の 午前9時 午後5時 当社の分取装置や高性能大型カラム 展示会出展スケジュールなど 情報が満載のウェブサイト ワイエムシィ 検 索 この冊子に記載の情報は 2017年4月現在の情報です 内容に関しては予告なく変更される場合がございますので あらかじめご了承ください 安全にご使用いただくために 必ず取扱説明書を熟読のうえ指示に従ってください 販売店 本社営業部 京都市下京区五条通烏丸西入醍醐町284番地 YMC烏丸五条ビル4F TEL FAX 東京営業部 東京都港区芝 クロスオフィス三田 601号 TEL FAX URL YMC CO., LTD. All Rights Reserved K-AGN

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