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1 Life Technologies NEXT 2011 CONTENTS New /Attune Attune Acoustic Focusing Cytometer P2 PRODUCT REVIEW 3100Mb! Ion PGM P6 10 No.9 E-Gel Go! & E-Gel Imager P3 MicroSEQ Ver.3.0 P7 microrna mirvana mirna mimics and inhibitors P4! P9 VILO SuperScript VILO Master Mix Gibco P5 P8 TECHNICAL REVIEW Qubit Protein Assay Kit PBS P10 SYBR Countess INFORMATION P5 P8 P12 P12 P12
2 Rat anti-mouse CD19 PE Cy5.5 Rat anti-mouse CD8 AF488 Mouse anti-mouse NK1.1 PE NEW /Attune Attune Acoustic Focusing Cytometer Acoustic focusing technology CV 10 * */ A 10 6 A. T and B Cell Subpopulations B 10 6 B. CD3+ gated CD4 and CD8 Subpopulations C 10 6 C. CD11 and NK1.1 Subpopulations Hamster anti-mouse CD3e Alexa Fluor Rat anti-mouse CD4 PE-Cy Rat anti-mouse CD11c APC-Cy 7 1. / Attune Acoustic Focusing Cytometer6 C57BL/6J6 (A) CD3e Alexa Fluor 647CD19 PE-Cy 5.5 (B) CD4 PE-Cy 7CD8 Alexa Fluor 448 (C) CD11c APC-Cy 7NK1.1 PE CD11b PE BL2-H GR-1 Pacific Orange VL3-H Pro Syn Euk Fluospheres Attune Acoustic Focusing Cytometer / Attune Acoustic Focusing Cytometer, RED LASR /
3 NEW E-Gel Go! & E-Gel Imager E-Gel Go! E-Gel ImagerE-Gel Blue Light UV E-Gel Go! 10μl % 2% 2 50bp 2kb E-Gel EX 1ng DNA E-Gel Go! Base 82X125X43mm E-Gel Go! 1% Gels 10pack G ,000 E-Gel Go! 1% Gels 20pack G ,000 E-Gel Go! 2% Gels 10pack G ,000 E-Gel Go! 2% Gels 20Pack G ,000 E-Gel Go! Base 1 G ,000 E-Gel Go! 1% Starter Kit 1Kit G4401ST 80,000 E-Gel Go! 2% Starter Kit 1Kit G4402ST 80,000 E-Gel Go! Portable Battery Pack 1 G ,000 E-Gel Go! Car Adaptor 1 G ,000 E-Gel Go! Travel Kit G4405 G4444 1Kit G4400TK 50,000 Starter kit10base 1kb Plus DNA E-Gel Imager 3 Universal UV/SYBR Qdot 625 E-Gel Go!E-Gel EX E-Gel 48 E-Gel 96 E-Gel Imager UV Light Base ,000 E-Gel Imager Blue Light Base ,000 E-Gel Imager Adaptor Base ,000 E-Gel Imager Band Excision Kit 1Kit ,000 E-Gel Imager Universal Filter ,000 E-Gel Imager Qdot 625 Filter ,000 E-Gel Imager UV/SYBR Filter ,000 E-Gel Imager Quantitation Dongle ,000 E-Gel Imager with UV Light Base ,000 E-Gel Imager with Blue Light Base ,000 E-Gel Imager with E-Gel Adaptor , E-Gel Go! BaseE-Gel ibase/e-gel Safe Imager 35.6cm X30.5cm X21cm V,50/60Hz,0.6A 540 UV Light/Blue Light Base 11.9cm X30.5cm X21cm V,50/60Hz,0.6A 540 3
4 NEW microrna mirvana mirna mimics and inhibitors mirvana mirna mimics and inhibitorsmirna mirna mirvana mirna mimics mirna mirnamirna mirvana mirna inhibitors mirnamirna Sanger Database, in vivoin vivo ready, Fold Change mirna mimic Neg mirna mimic Neg mirna mimic Neg mirna mimic Neg mirna mimic Neg mirna mimic reporter 1 reporter 2 reporter 3 reporter 4 reporter 5 reporter 6 FW RV Neg mrna upregulation % mir 122 mir122 mir122 mirna inhibitor NT Not transfected Neg1 Negative Control #1 NT Neg1 mir NT Neg1 mir NT Neg1 mir NT AldoA Hfe2 Slc35a4 Lass6 Neg1 1 mirvana mirna mimic microrna mirvana mirna mimic microrna mirvana mirna mimic mirna mimic 1 microrna microrna mirvana mirna Inhibitors in vivo mirna Balb-C mirvana mirna inhibitor mir122negative Control in vivoinvivofectamine 2.07 mg/kg 3 mirna inhibitor 4 AldoA, Hfe2, Slc35a4 and Lass6 mir122 mirna inhibitor Negative control mrna AldoA 3.5, Hfe2 2, Slc35a4 3, Lass6 4 mirvana mirna inhibitor Invivofectamine 2.0 Reagent mir122 mir122 mirvana mirna mimic mirvana mirna mimic 5nmol ,000 mirvana mirna mimic 20nmol ,000 mirvana mirna mimic, in vivo Ready 250nmol ,000 Customer-defined mirvana mirna mimic 5nmol ,000 Customer-defined mirvana mirna mimic 20nmol ,000 Customer-defined mirvana mirna mimic, in vivo ready 250nmol ,000 mirvana mirna mimic, Custom 20nmol mirvana mirna mimic, Custom 250nmol mirvana mirna mimics Library* 0.25nmol each mirvana mirna mimics Library* 1nmol each mirvana mirna inhibitor mirvana mirna inhibitor 5nmol ,000 mirvana mirna inhibitor 20nmol ,000 mirvana mirna inhibitor, in vivo ready 250nmol ,000 Customer-defined mirvana mirna inhibitor 5nmol ,000 Customer-defined mirvana mirna inhibitor 20nmol ,000 Customer-defined mirvana mirna inhibitor, in vivo ready 250nmol ,000 mirvana mirna inhibitor, Custom 20nmol mirvana mirna inhibitor, Custom 250nmol mirvana mirna inhibitor Library* 0.25nmol each mirvana mirna inhibitor Library* 1nmol each Negative Control, Positive Control * Sanger database v17 mirna 4 mirvana mirna mimics and inhibitors
5 NEW VILO SuperScript VILO Master Mix SuperScript VILO Master MixVILOVILO RNA RNA cdna PCR 3 4 SuperScript PCR RNA 1pg 2.5μg 5020μl ,000 SuperScript VILO Master Mix 25020μl , μl ,000 SYBR TaqMan TaqMan 38,500 SYBR SYBR 15,000 PCRTaqMan? Fast SYBR Power SYBR TaqMan 50% 50% OFF Fast TaqMan Fast Advanced Master Mix P2-XD7WEV Fast SYBR CAMP 77,000 38,500 Fast SYBR Green Master Mix,5mL 1 500TaqMan Fast Advanced Master Mix, 1mL 1100 TaqMan GeneExpression Assays, Extra Small 275* 20μL Power SYBR CAMP 77,000 38,500 Power SYBR Green Master Mix,5mL 1 500TaqMan Fast Advanced Master Mix, 1mL 1100 TaqMan GeneExpression Assays, Extra Small 275* 20μL **TaqMan RNA PureLink RNA Mini kit RNA Qubit 2.0 Fluoromter 5
6 PRODUCT REVIEW 3100Mb! Ion PGM Ion PGM 1 Ion PGM 10Mb10100Mb bp bp denovo RNA 314 JAN Mb 100bp 316 JUL Mb 100bp 318* Q Gb 200bp Ion PGM Ion PGM 1 Ion Xpress Fragment Library Kit Ion OneTouch DNA Duo PCR 60% % 4 Ion PGM 30 bp 1 100bp 2 Torrent Suite v 1.4 Ion 314 chip 30Ion 316 chip 60 WORKFLOW TIMES 3.5 HOURS 3.5 HOURS Only minutes of hands-on time 2.5 HOURS Under one hour for short reads 1.0 HOURS The PGM takes the shortest time to generate genomic data. JUNJIE QIN BGI, CHINA ION KITS ION ONE-TOUCH SYSTEM ION PGM SEQUENCER TORRENT SERVER Ion PGM Nature 7 21NatureIon PGM Ion PGM CMOS IC 3 Postlight 2 O104 H4 Ion Personal Genome Machine Nature *
7 PRODUCT REVIEW MicroSEQ Ver.3.0 MicroSEQ 4Version 3.0 MicroSEQ 3500 / 5 Specimen % 211 and Curvibacter lanceolatus* ATCC Genetic Distance 0.31% Hydrogenophaga pseudoflava* ATCC Hydrogenophaga taeniospiralis* ATCC Hydrogenophaga flava* ATCC Hydrogenophaga palleronii* ATCC Malikia spinosa* ATCC Acidovorax konjaci* ATCC Variovorax paradoxus* ATCC Curvibacter lanceolatus* ATCC MicroSEQ 3500 BLAST MicroSEQ 4000! 5 16S S 1262 D Fast PCR PCR MicroSEQ DNA MicroSEQ V3.0 BUNDLE ,250,000 MicroSEQ V3.0 BUNDLE ,980,000 MicroSEQ MicroSEQ V3.0 BUNDLE LITE ,250,000 MicroSEQ V3.0 BUNDLE LITE ,000,000 MicroSEQ MicroSEQ v2 MicroSEQ V ,980,000 MicroSEQ V ,980,
8 NEW Gibco Gibco ISO IGF-1, Recombinant Human 20μg PHG ,000 IGF-1, Recombinant Human 100μg PHG ,000 LIF, Recombinant Human 1000μg PHC ,000 IL-9, Recombinant Human 10μg PHC ,000 IL-9, Recombinant Human 100μg PHC ,000 IL-28A, Recombinant Human 10μg PHC ,000 IL-28B, Recombinant Human 10μg PHC ,000 LEFTY, Recombinant Human 10μg PHC ,000 HGF, Recombinant Human 10μg PHG ,000 HGF, Recombinant Human 100μg PHG ,000 CYSTATIN C, Recombinant Human 10μg PHP ,000 HGH, Recombinant Human 10μg PHH ,000 Countess P Countess 50Trypan Blue 0.4% 2 ml 1 C , ,000 Countess Countess 2 Trypan Blue 0.4% 50 C , C ,000 1,250 C ,000 2,500 C ,000 5,000 C ,000 8
9 PRODUCT REVIEW! Gateway Gateway BP, LR 1kbp 230,000 3 TOPO TOPO 230, ,000 4 Gateway Gateway 50 5 Gateway Gateway 200, /bp 3 63,000 2 T7, pbad 500,000 5L 2 Bac-to-Bac 1,600,000 1L 7 FreeStyle FreeStyle ,000 5L 1x10e4 10e6 TU/ml 560, x10e8 PFU 680,000 7 BacMam 9,000 1,400, cdna total RNA 900,000 8 SelectScreen Lantha Screen Eu kinase Binding Assay SelectScreen Select Screen herg 250 IC50 ATP GPCR QT herg mg ,400/ 5mg, 90% 287, TEL FAX jpcustom invitrogen.com 9
10 TECHNICAL REVIEW Qubit Protein Assay Kit PBS QubitDNA RNA Qubit Protein Assay Kit Qubit Protein Assay Kit Qubit 2.0 Fluorometer Qubit 2.0 Fluorometer 3 Qubit 1 PBS PBSPBS TE buffer PBS5 20μL 200ul Qubit PBS PBS RFU Water TE buffer PBS 5 μl 10 μl 20 μl 2 PBS Qubit Protein Assay TE buffer BSA PBS PBSPBS QubitPBS 0 μl TE buffer PBS PBS μg/ml Sample A Sample B Sample C PBS 0 μl PBS 5 μl PBS 10 μl 3 Triton X-100 QubitSDS 0.2% Triton X-100 Tween %* Triton X-100 * 10μl Triton X % milli-q Triton X-100 Triton X-100 Triton X (RFU) μl Triton X-100 (%) 0 μg/ml 25 μg/ml 50 μg/ml 100 μg/ml 200 μg/ml 400 μg/ml 10
11 TECHNICAL REVIEW 4 Triton X-100 PBSQubit Protein Assay Triton X-100 Triton X10μl Triton X 10μl Qubit Triton X-100 Triton X Triton X 0.013% Qubit "Standard Incorrect" Triton X Qubit μg/ml Sample 0 Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 TritonX 0 % % % % Qubit Qubit Protein Assay Kit PBS 10μlTriton X % Qubit 2.0 Fluorometer 1 Q , Q ,900 Qubit Protein Assay Kit 500 Q ,700 Qubit Assay Tubes 500 Q ,000 Qubit, Protein Assay Kit 100, Tube 1 Q33211NSK 180,000 Q.?? A. Q.? A. BSA Q.? A. DTT 2-mercaptoethanol 11
12 INFORMATION American Society for Human Genetics ASHG Palais des Congres de Montreal QUIZ Life Technologies! 10Life Technologies Q? A! Invitrogen Corporation Applied Biosystems Inc Invitrogen Applied Biosystems PCR DNA The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. 2011, Life Technologies Japan Ltd. All rights reserved. Printed in Japan TEL FAX
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