Immunoassay_Brochure_1002.indd

Transcription

1 ELISA

2 Novex Life Technologies ELISA Novex ELISA 3 ELISA 5 14 ready-to-use 1 * 10 pg/ml 10 pg/ml 10 pg/ml * pg/ml pg/ml** 5 2,000 pg/ml * HRP-TMB HRP-TMB R-PE Luminex Luminex 100 /200 MAGPIX FLEXMAP 3D System *Novex ELISA **ELISA ELISA -HRP 96 HRP SAV-R-PE Immunoassay Selection Guide ELISA Novex 2 Life Technologies Immunoassays

3 Novex ELISA easy-touse Buffer Set for Antibody Pairs 10 ELISA 5 HRP Assay Buffer Set CNB ELISA A B Akt [ps473] CHO0115 C-Met (soluble) CHO0315 C-Met (total) CHO0285 ERK1/2 [ptpy185/187] CHO0095 IFN-γ 10 plates CHC1233 IL-1β 10 plates CHC1213 IL-1ra 10 plates CHC1183 IL-2 10 plates CHC1243 IL-4 10 plates CHC1283 IL-6 10 plates CHC1263 IL-8 10 plates CHC1303 IL plates CHC1323 IL-12 (p40/p70) 10 plates CHC1563 IL-23 heterodimer 10 plates CHC2493 IP plates CHC2363 MCP-1 10 plates CHC1013 MIP-1β 10 plates CHC2293 MMP-3 10 plates CHC1543 SAA 10 plates CHA2513 TGF-β1 10 plates CHC1683 TNF-α 10 plates CHC1753 VEGF 10 plates CHG0113 Life Technologies Antibody pair kits 3

4 IFN- 10 plates CMC4033 Ig isotyping (6 targets) 6 plates IL-1 10 plates CMC0813 IL-4 10 plates CMC0043 IL-5 10 plates CMC0053 IL-6 10 plates CMC0063 IL plates CMC0103 IL plates CMC0123 IL plates CMC2223 IL plates CMC2493 KC 10 plates CMC1063 TGF plates CHC1683 TNF- 10 plates CMC3013 IL-2 10 plates CRC0023 IL-6 10 plates CRC0063 IL plates CRC0103 IL plates CRC0133 TGF plates CHC1683 TNF- 10 plates CRC3013 IFN- 10 plates CSC4033 IL-2 10 plates CSC1243 IL-4 10 plates CSC1283 IL plates CSC0103 TNF- 10 plates CSC1753 TGF plates CHC1683 IFN- 10 plates CAC1233 The Antibody Pair Buffer Set includes the following components (sufficient reagents for 10 plates): Assay Buffer (5x) 200 ml x 1 bottle Coating Buffer A Coating Buffer B 100 ml x 1 bottle 100 ml x 1 bottle Wash Buffer (25x) 100 ml x 3 bottles Stabilized Chromogen 25 ml x 4 bottles Stop Solution 100 ml x 1 bottle CNB0011 Assay Buffer (5x) 200 ml x 1 bottle DS98200 Coating Buffer A 100 ml x 1 bottle CB07100 Coating Buffer B 100 ml x 1 bottle CB01100 Wash Buffer (25x) 100 ml x 1 bottle WB01 Wash Buffer (25x), Bulk Pack 1.0 L bottle WB02 Stabilized Chromogen 25 ml x 1 bottle SB01 Stabilized Chromogen, Bulk Pack 1.0 L bottle SB02 Stop Solution 25 ml bottle SS03 Stop Solution, Bulk Pack 1.0 L bottle SS04 4 Life Technologies Antibody pair kits

5 ELISA Novex ELISA 1 ELISA Novex ELISA ELISA ELISA ELISA Chemi ELISA * 10 pg/ml 1 pg/ml 1 pg/ml * pg/ml pg/ml 0.5 2,500 pg/ml * HRP-TMB HRP-TMB AP-CSPD * Novex ELISA 4 4 HRP nm 1 Novex ELISA4 96 HRP HRP HRP 450 nm Life Technologies ELISA kits 5

6 ELISA ELISA ISO ready-to-use ELISAELISAELISA 2 Novex ELISA NIBSC CV 10% CV 10% EDTA 10%IL-23 Novex Human IL-23 Heterodimer ELISA Kit KHC % EDTA 88% 83% 86% RPMI + 10% 116%* DMEM + 10% 100% *100% 6 Life Technologies ELISA kits

7 OD 450 nm Life Technologies MMP9 (ng/ml) 2 Novex ELISA Kit KHC3061 MMP9 IFN-γ (IU/mL) A C1 C2 C3 C4 B C D E F G H I J K ELISA 3 4 C1 C4 20% OD 450 nm IL-6 0 1,000 2,000 3,000 (pg/ml) 4 Novex Rat IL-6 ELISA Kit KRC0061 EDTATC EDTA TC ACC-1 [ps79] KHO1071 Adiponectin KHP0041 Aggrecan KAP1461 Akt [pt308] KHO0201 Akt1 (total) KHO0531 Akt1 [ps473] KHO0541 Angiopoietin-2 KHC1641 APO/FAS KHS9501 APP KHB0051 APRIL KHC3051 Aggregated A KHB3491 A 40 A 42 KHB3481 KHB3482 KHB3441 KHB3442 A 42 (ultrasensitive) KHB3544 -catenin (total) KHO1211 Caspase-3 KHO1091 CD23 KAS0251 CD40L (soluble) KHS4001 c-met (soluble) KHO2031 c-met (total) KHO0251 c-met [pypypy1230/34/35] KHO0281 c-myc (total) KHO2041 C-reactive protein (CRP) KHA0031 CREB KHO0231 CREB [ps133] KHO0241 Cytochrome c KHO1051 EGF KHG0061 KHG0062 EGFR KHR9061 EGFR [py1173] KHR9071 EGFR [py1068] KHR9081 ELAM-1 (soluble) KHS2011 Eotaxin/CCL11 KAC2231 Estradiol KAQ0621 Fas ligand (soluble) KHS9521 FGF basic G-CSF KHG0021 KHG0022 KHC2031 KHC2032 GM-CSF KHC2011 Growth hormone KAQ1081 Her2 (total) KHO0701 HGF KAC2211 Histone H3 (total) KHO0661 Histone H3 [ps10] KHO0671 HSP27 (total) KHO0331 HSP27 (ps82) KHO0341 ICAM-1 (soluble) KHS5411 IFN- KHC4011 IFN- (multi subtype) KHC4032 IFN- * KAC1231 Life Technologies ELISA kits 7

8 IFN- KHC4021 KHC4021C KHC4022 IgG1, 2, 3, 4* IL-1ra KAC1181 IL-1 KAC1191 IL-1 * KAC1211 IL-1 KHC0011 KHC0011C KHC0012 IL-1 (ultrasensitive) KHC0014 IL-2* KAC1241 IL-2 KHC0021 KHC0021C KHC0022 IL-2R KHR0021 IL-3 KHC0031 IL-4* KAC1281 IL-4 KHC0041 IL-4 (ultrasensitive) KHC0044 KHC0043 IL-5 KHC0051 IL-6* KAC1261 IL-6 KHC0061 KHC0061C KHC0062 IL-6R KHR0061 IL-8* KAC1301 IL-8 KHC0081 KHC0081C KHC0082 IL-8 (ultrasensitive) KHC0084 KHC0083 IL-10* KAC1321 IL-10 IL-10 (ultrasensitive) KHC0101 KHC0101C KHC0102 KHC0104 KHC0104C KHC0103 IL-12 (p40/p70) KAC1561 KHC0121 KHC0122 IL-12 (p70) KAC1568 IL-13 IL-13 (ultrasensitive) IL-15 KHC0131 KHC0132 KHC0134 KHC0133 KHC0151 KHC0152 IL-16 KHC0161 IL-17 KAC1591 IL-23 heterodimer KHC0231 Insulin KAQ1251 IP-10 KAC2361 IR (total) KHR9111 IR [py1158] KHR9121 IR [pypy1162/1163] KHR9131 * IR [py1334] KHR9161 I B (total) KHO0211 I B [ps32] KHO0221 JNK 1/2 (total) KHO0121 JNK 1/2 [ptpy183/185] KHO0131 Leptin KAC2281 Leukotriene B4 KHL1741 Leukotriene E4 KHL1761 LIF-HILDA KAC1351 MCP-1/CCL2 KHC1011 KHC1012 MIP-1 /CCL3 KAC2201 MIP-1 /CCL4 KAC2291 MMP-2 KHC3081 KHC3082 MMP-3 KAC1541 MMP-9 KHC3061 Osteocalcin KAQ1381 p21 (total) KHO5421 p27kip1 KHO5321 p38 MAPK (total) KHO0061 p38 MAPK [ptpy180/182] KHO0071 p70-s6k [pt389] KHO0581 PAI-1 KHC3071 PARP (cleaved) KHO0741 PRAS40 (total) KHO0411 PRAS40 [pt246] KHO0421 Prostaglandin E2 KHL1701 P-selectin (soluble) KHS2021 RANTES/CCL5* KAC1511 RANTES/CCL5 KHC1031 KHC1032 RBP4 KHP0081 Resistin KHP0051 Retinoblastoma (Rb) (total) KHO0011 Retinoblastoma (Rb) [pt821] KHO0021 SAA (serum amyloid A) KHA0011 KHA0011C KHA0012 SMAD2 (total) KHO2021 SMAD2 [ps465/467] KHO2011 STAT5a (total) KHO0751 STAT5a [py694] KHO0761 STAT5b [py699] KHO5721 Tau (total) KHB0041 KHB0042 Tau [ps396] KHB7031 Tau [ps199] KHB7041 Tau [pt231] KHB8051 Tau [pt181] KHO0631 TGF- 1 (activated/treated) KAC1688 TIMP-1 KHC1491 TNF-RI KAC1761 TNF-RII KAC1771 TNF- * KAC Life Technologies ELISA kits

9 TNF- KHC3011 KHC3011C KHC3012 TNF- (ultrasensitive) KHC3013 KHC3014 TNF- KHC3014C VCAM-1 (soluble) KHT0601 VEGF KHG0111 KHG0112 -Synuclein KHB0061 JAK2 (total) KHO5521 JAK2 [pypy1007/1008] KHO5621 NF B P65 (total) KHO0371 4EBP-1 (total) KHO0681 4EBP-1 [pt46] KHO0691 Akt KHO0101 Akt [ps473] KHO0111 AMPK [pt172] KHO0651 ERK1/2 KHO0081 ERK1/2 [ptpy185/187] KHO0091 FAK (total) KHO0431 FAK [py397] KHO0441 GSK-3 (total) KHO0451 GSK-3 [ps9] KHO0461 IGF-1R [pypy1135/1136] KHO0501 IRS-1 (total) KHO0511 IRS-1 [ps312] KHO0521 p70-s6k (total) KHO0571 STAT3 [py705] KHO0481 IFN- IL-2 KPC4021 KPC4022 KPC0021 KPC0022 IL-4 KPC0041 IL-6 KPC0061 IL-8 KPC0081 IL-10 KPC0101 IL-12 KPC0121 IL-12 (p70) KPC9121 TNF- KPC3011 KPC3012 Adiponectin (ACRP30) KMP0041 A 42 KMB3441 IFN- KMC4011 IFN- KMC4041 IFN- KMC4021 KMC4021C KMC4022 Ig 6 plates * IL-1 KMC0011 KMC0011C KMC0012 IL-2 KMC0021 KMC0021C KMC0022 IL-4 KMC0041 KMC0042 IL-5 KMC0051 IL-6 IL-10 IL-12 KMC0061 KMC0061C KMC0062 KMC0101 KMC0102 KMC0121 KMC0121C KMC0122 IL-12 (p70) KMC9121 IL-13 KMC2221 IL-17 KMC3021 IL-23 KMC0231 KC KMC1061 Leptin KMC2281 MCP-1/CCL2 KMC1011 KMC1012 PRAS40 (total) KMO0411 PRAS40 [pt246] KMO0421 RANTES/CCL5 KMC1031 SAA (serum amyloid A) KMA0021 Tau KMB7011 Tau [ps199] KMB7041 TNF- VEGF KMC3011 KMC3011C KMC3012 KMG0111 KMG0112 A 40 [1-40] KMB3481 Adiponectin (ACRP30) KRP0041 Growth hormone KRC5311 IFN- IL-1 KRC4021 KRC4022 KRC0011 KRC0011C KRC0012 IL-2 KRC0021 KRC0022 IL-4 KRC0041 IL-6 IL-10 IL tests KRC0061 KRC0062 KRC0061C KRC0101 KRC0102 KRC0121 KRC0122 Life Technologies ELISA kits 9

10 IL-12 (p70) KRC2371 IL-13 KRC0131 KRC0132 IL-18 KRC2341 Leptin KRC2281 MCP-1/CCL2 KRC1011 KRC1012 MIP-2 KRC1021 KRC1022 RANTES/CCL5 KRC1031 TNF- KRC3011 KRC3011C KRC3012 IFN- KSC4021 KSC4022 IL-4 KSC0041 KSC0042 IL-8 KSC0081 KSC0082 IL-10 KSC0101 KSC0102 TNF- KSC3011 KSC3012 IFN- KBC1231 SAA (serum amyloid A) KAA0021 ELISA ELISA 5 Chemi ELISAwww.lifetechnologies.com/chemielisas 10,000 1, ELISA ELISA ELISA IL-1 KHC0019 IL-6 KHC0069 TARC KHC3089 TNF- KRC ,000 10,000 (pg/ml) 5 Human IL-1 Chemi ELISA Kit KHC0019Human IL-1 Ultrasensitive ELISA Kit KHC0014 Human IL-1 ELISA Kit KHC0011 1pg/mLELISA Life Technologies Chemiluminescent ELISA kits

11 ELISA Life Technologies A Novex ELISA ELISA 8 ELISA Standard Diluent Buffer APP %RPMI Standard Diluent Buffer % pg/ml pg/ml % 1/ / / / pg/ml pg/ml % 1/ / / / OD ,200 1,800 (pg/ml) 6 Human Tau (Total) ELISA Kit KHB ,000 pg/ml 12 pg/ml OD 450 nm Hu Hu ,000 1,200 A 40 A 42 KHB3481 KHB3482 KHB3441 KHB3442 A 42 (ultrasensitive) KHB3544 Aggregated A KHB3491 APP KHB0051 -Synuclein KHB0061 Tau (total) KHB0041 KHB0042 Tau [ps199] KHB7041 Tau [ps396] KHB7031 Tau [pt181] KHO0631 Tau [pt231] NEW KHB8051 A 40 KMB3481 A 42 KMB3441 Tau (total) KMB7011 Tau [ps199] KMB7041 pg/ml Hu 7Hu Standard Diluent Buffer OD Hu Life Technologies Neurobiology ELISA kits 11

12 ELISA Novex ELISA ELISA A B OD 450 nm (µg) 8 ELISA A STAT 5a [py694] Human ELISA Kit KHO0761 B NuPAGE Novex NP0321 4E-BP1 (total) Human, mouse, rat KHO0681 4E-BP1 [pt46] Human, mouse, rat KHO0691 ACC1 (total) Human KHO1071 ACC1 [ps79] Human KHO1061 Akt (total) Human, mouse, rat KHO0101 Akt [ps473] Human, mouse, rat KHO0111 Akt1 [ps473] Human KHO0541 (ultrasensitive) Akt [pt308] Human KHO0201 AMPK [pt172] Human, mouse, rat KHO0651 -Catenin (total) Human KHO1211 Caspase-3 (active) Human KHO1091 c-myc Human KHO2041 CREB (total) Human, mouse KHO0231 CREB [ps133] Human, mouse KHO0241 Cytochrome c Human KHO1051 EGFR (full-length) Human KHR9061 EGFR [py1068] Human KHR9081 EGFR [py1173] Human KHR9071 ERK1/2 (total) Human, mouse, rat KHO0081 ERK1/2 [ptpy185/187] Human, mouse, rat KHO0091 FAK (total) Human, mouse, rat KHO0431 FAK [py397] Human, mouse KHO0441 GSK-3 (total) Human, mouse, rat KHO0451 GSK-3 [ps9] Human, mouse, rat KHO0461 HER2 (total) Human KHO0701 Histone H3 (total) Human KHO0661 Histone H3 [ps10] Human, mouse, rat KHO0671 HSP27 (total) Human KHO0331 HSP27 [ps82] Human KHO0341 IGF-1R [pypy1135/1136] Human, mouse, rat KHO0501 I B (total) Human KHO0211 I B [ps32] Human KHO0221 IR (total) ( -subunit) Human, mouse, rat KHR9111 IR [py1158] Human, mouse, rat KHR9121 IR [py1334] Human, mouse, rat KHR9161 IR [pypy1162/1163] Human, mouse, rat KHR9131 IRS-1 (total) Human, mouse, rat KHO Life Technologies phosphoelisa kits

13 IRS-1 [ps312] Human, mouse, rat KHO0521 JAK2 (total) Human, mouse KHO5521 JAK2 [pypy1007/1008] Human, mouse KHO5621 JNK 1/2 (total) Human, mouse KHO0121 JNK 1/2 [ptpy183/185] Human, mouse KHO0131 c-met (total) Human KHO0251 c-met Human KHO0281 [pypypy1230/1234/1235] NF- BP65 [total] Human, mouse KHO0371 p21 Waf1/Cip1 (total) Human KHO5421 p27kip1 Human, mouse, rat KHO5321 p38 MAPK (total) Human, mouse, rat KHO0061 p38 MAPK [ptpy180/182] Human, mouse, rat KHO0071 p70-s6k (total) Human, mouse, rat KHO0571 p70-s6k [pt389] Human KHO0581 PARP (cleaved) [214/215] Human KHO0741 PRAS40 (total) Human KHO0411 PRAS40 (total), mouse Human, mouse, rat KMO0411 PRAS40 [pt246] Human KHO0421 PRAS40 [pt246], mouse Human, mouse, rat KMO0421 Rb (total) Human KHO0011 Rb [pt821] Human KHO0021 SMAD2 (total) Human KHO2021 SMAD2 [psps465/467] Human KHO2011 STAT3 [py705] Human KHO0481 STAT5a [py694] Human KHO0761 STAT5a (total) Human KHO0571 STAT5b [py699] Human KHO5721 Tau (total) Human Human KHB0041 KHB0042 Tau [ps199] Human KHB7041 Tau [ps396] Human KHB7031 Tau [pt181] Human KHO0631 Tau [pt231] Human KHB8051 Tau (total) Mouse KMB7011 Tau [ps199] Mouse KMB7041 * Cell Extraction Buffer 100 ml FNN0011 NP40 Lysis Buffer 100 ml FNN0021 Tissue Extraction Reagent I 100 ml FNN0071 Denaturing Cell Extraction Buffer 100 ml FNN0091 * Life Technologies phosphoelisa kits 13

14 Luminex Novex Novex Luminex xmap ELISA Novex ELISA 5 50 L 100 xmap Novex ELISA 6 Life Technologies Luminex xmap Novex Luminex xponent ELISA (pg/ml) 1, y = x R 2 = ,000 Novex (pg/ml) 9 ELISA Novex GM-CSF ELISA Novex Novex ELISA9 * ELISA90% 10 pg/ml 2 SD CV 10% CV 10% R2 > 0.99 *Novex 14 Life Technologies Multiplex assays

15 1,400 1,200 1, IL-1ϐ IL-10 IL-6 GM-CSF IL-5 IFN-γ TNF-α IL-2 IL-4 IL CV 10% Novex Cytokine Human Magnetic 10-Plex Panel LHC0001M A 100% B 100% 79% 75% 75% 66% 50% 25% 0% FGF- 1,IL-1b G-CSF IL-10 IL-13 IL-6 IL-12 IL-17 MIP-1a GM-CSF MIP-1b MCP-1 Novex 2 3 IL-15 VEGF IFN-g IL-1RA TNF-a IL-2 IL-7 IP-10 IL-4 IL-8 11 Novex 23Novex 2 MAGPIX A B 50% 25% 0% Novex 2 17% 3 6 Cytokine Human 10-Plex Panel LHC MFI IL-1 IL-2 IL-4 IL-5 IL-6 IL-8 IL-10 TNF- IFN- GM-CSF IL-1 6, IL , IL , IL , IL , IL , IL , TNF , IFN , GM-CSF ,932 Life Technologies Multiplex assays 15

16 Luminex Luminex xmap Novex Luminex Luminex ELISA Novex ELISA 13 Novex 96 Novex -R-PE Luminex Novex 2 1 -R-PE 30 Luminex 13 Novex 16 Life Technologies Multiplex assays

17 Novex Luminex xmap Novex Novex Novex A14179 Adiponectin Polystyrene LHP0041 Aggregated A Polystyrene LHB3491 Aggregated -synuclein Polystyrene LHB0071 A 40 Polystyrene LHB3481 A 42 Polystyrene LHB3441 BDNF Polystyrene LHC7071 CD30 (soluble) Polystyrene LHS6031 C-reactive protein (CRP) Polystyrene LHP0031 DR5 Polystyrene LHR0051 EGF Polystyrene LHG0061 EGFR (total) Polystyrene LHR9061 EGFR [py1068] Polystyrene LHR9081 Eotaxin/CCL11 Polystyrene LHC2231 FGF basic Polystyrene LHG0021 G-CSF Polystyrene LHC2031 GDNF Polystyrene LHC7041 GM-CSF Polystyrene LHC2011 GRO- Polystyrene LHC1061 HGF Polystyrene LHG0071 IFN- Polystyrene LHC4011 IFN- Polystyrene LHC4031 IGF-1R [pypy1135/1136] Polystyrene LHO0501 IL-10 Polystyrene LHC0101 IL-12 (p40/p70) Polystyrene LHC0121 IL-12 (p70) Polystyrene LHC9121 IL-13 Polystyrene LHC0131 IL-15 Polystyrene LHC0151 IL-16 Polystyrene LHC0161 IL-17 Polystyrene LHC0171 IL-1ra Polystyrene LHC0711 IL-1 Polystyrene LHC0811 IL-1 Polystyrene LHC0011 IL-1 (ultrasensitive) Polystyrene LHC0013 IL-2 Polystyrene LHC0021 IL-2R Polystyrene LHR0021 IL-3 Polystyrene LHC0031 IL-4 Polystyrene LHC0041 IL-5 Polystyrene LHC0051 IL-6 Polystyrene Polystyrene LHC0061 LHC0063 IL-6R (soluble) Polystyrene LHR0061 IL-7 Polystyrene LHC0071 IL-8 Polystyrene Polystyrene LHC0081 LHC0083 Insulin Polystyrene LHP0021 IP-10 Polystyrene LHC1081 IRS-1 (total) Polystyrene LHO0511 Leptin Polystyrene LHP0011 MCP-1/CCL2 Polystyrene LHC1011 MCP-2/CCL8 Polystyrene LHC1111 MCP-3/CCL7 Polystyrene LHC1571 MIG/CXCL9 Polystyrene LHC1091 MIP-1 /CCL3 Polystyrene LHC1021 MIP-1 /CCL4 Polystyrene LHC1051 PDGF-BB Polystyrene LHG0041 RANTES/CCL5 Polystyrene LHC1031 Resistin Polystyrene LHP0051 Life Technologies Multiplex assays 17

18 SAA (serum amyloid A) Polystyrene LHP0061 Tau (total) Polystyrene LHB0041 Tau [pt181] Polystyrene LHB7051 TGF- 1 (activated/treated) Polystyrene LHG0121 TNF-RI Polystyrene LHC3021 TNF-RII Polystyrene LHC3031 TNF- Polystyrene LHC3011 TNF- (ultrasensitive) Polystyrene LHC3013 VEGF Polystyrene LHG0111 JNK 1/2 (total) Polystyrene LHO0071 JNK 1/2 [ptpy183/185] Polystyrene LHO0081 p38 MAPK (total) Polystyrene LHO0031 p38 MAPK [ptpy180/182] Polystyrene LHO0041 Akt (total) Polystyrene LHO0091 Akt [ps473] Polystyrene LHO0101 GSK-3 (total) Polystyrene LHO0451 GSK-3 [ps9] Polystyrene LHO0461 IGF-1R (total) Polystyrene LHO0491 IR (total) Polystyrene LHR9111 IR [pypy1162/1163] Polystyrene LHR9131 p70s6k (total) Polystyrene LHO0181 p70s6k [ptps421/424] Polystyrene LHO0191 PRAS40 (total) Polystyrene LHO0411 PRAS40 [pt246] Polystyrene LHO0421 G-CSF Polystyrene LPC2031 IFN- Polystyrene LPC4031 IL-10 Polystyrene LPC0101 IL-12 Polystyrene LPC0121 IL-17 Polystyrene LPC0171 IL-2 Polystyrene LPC0021 IL-4 Polystyrene LPC0041 IL-6 Polystyrene LPC0061 IL-8 Polystyrene LPC0081 IP-10 Magnetic LPC1171M MCP-1/CCL2 Polystyrene LPC1011 MIP-1 /CCL3 Polystyrene LPC1021 MIP-1 /CCL4 Polystyrene LPC1051 RANTES/CCL5 Polystyrene LPC1031 TNF-α Polystyrene LPC3011 FGF basic Polystyrene LMG0021 G-CSF Polystyrene LMC2031 GM-CSF Polystyrene LMC2011 IFN- Polystyrene LMC4031 IL-10 Polystyrene LMC0101 IL-12 (p40/p70) Polystyrene LMC0121 IL-12 (p70) Polystyrene LMC9121 IL-13 Polystyrene LMC0131 IL-17 Polystyrene LMC0171 IL-1 Polystyrene LMC0811 IL-1 Polystyrene LMC0011 IL-2 Polystyrene LMC0021 IL-4 Polystyrene LMC0041 IL-5 Polystyrene LMC0051 IL-6 Polystyrene LMC0061 IP-10 Polystyrene LMC1081 KC Polystyrene LMC1061 MCP-1/CCL2 Polystyrene LMC1011 MIG/CXCL9 Polystyrene LMC1091 MIP-1 /CCL3 Polystyrene LMC1021 MIP-1 /CCL4 Polystyrene LMC1051 RANTES/CCL5 Polystyrene LMC1031 TNF- Polystyrene LMC3011 VEGF Polystyrene LMG0111 GM-CSF Polystyrene LRC2011 IFN- Polystyrene LRC4031 IL-10 Polystyrene LRC0101 IL-12 (p40/p70) Polystyrene LRC0121 IL-1 Polystyrene LRC0811 IL-1 Polystyrene LRC0011 IL-2 Polystyrene LRC0021 IL-4 Polystyrene LRC0041 IL-6 Polystyrene LRC0061 TNF- Polystyrene LRC3011 Human Extracellular Protein Buffer Human, LHB0001 Reagent Kit monkey Intracellular Protein Buffer Reagent Human LHB0002 Kit Human Ultrasensitive Cytokine Buffer Human LHB0003 Reagent Kit Mouse Extracellular Protein Buffer Mouse, rat LMB0001 Reagent Kit Multispecies Growth Factor Buffer Human, LMB0002 Reagent Kit mouse, rat Neuroscience Buffer Reagent Kit Human LNB Life Technologies Multiplex assays: singleplex kits

19 Hu Th1/Th2/Th17 Magnetic 8-plex Panel IL-2, IL-4, IL-5, IL-9, IL-10, IL-13, Il-17, IFN- Magnetic LHC0015M NEW Cytokine Human Magnetic 10-Plex Panel GM-CSF, IFN-, IL-1, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, TNF- Magnetic LHC0001M Inflammatory Cytokine Human Magnetic GM-CSF, IL-1, IL-6, IL-8, TNF- Magnetic LHC0003M 5-Plex Panel Cytokine Human Magnetic 25-Plex Panel Eotaxin, GM-CSF, IFN-, IFN-, IL-1, IL-1ra, IL-2, IL-2R, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40/p70), IL-13, IL-15, IL-17, IP-10, MCP-1, MIG, MIP-1, MIP- 1, RANTES, TNF- Magnetic LHC0009M Cytokine Human Magnetic 30-Plex Panel EGF, eotaxin, FGF basic, G-CSF, GM-CSF, HGF, IFN-, IFN-, IL-1, IL-1ra, IL- 2, IL-2R, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40/p70), IL-13, IL-15, IL-17, IP-10, MCP-1, MIG, MIP-1, MIP-1, RANTES, TNF-, VEGF Magnetic LHC6003M Cytokine Ultrasensitive Human Magnetic 10-Plex Panel GM-CSF, IFN-, IL-1, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, TNF- Magnetic LHC6004M Cytokine Human 10-Plex Panel GM-CSF, IFN-, IL-1, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, TNF- Polystyrene LHC0001 Th1/Th2 Cytokine Human 5-Plex Panel IFN-, IL-2, IL-4, IL-5, IL-10 Polystyrene LHC0002 Inflammatory Cytokine Human 5-Plex GM-CSF, IL-1, IL-6, IL-8, TNF- Polystyrene LHC0003 Panel Growth Factor Human 4-Plex Panel EGF, FGF basic, G-CSF, VEGF Polystyrene LHC0004 Chemokine Human 5-Plex Panel Eotaxin, MCP-1, MIP-1, MIP-1, RANTES Polystyrene LHC0005 Death Receptor Human 3-Plex Panel TNF-RI, TNF-RII, DR5 Polystyrene LHC0006 Cytokine II Human 5-Plex Panel IFN-, IL-12 (p40/p70), IL-13, IL-15, IL-17 Polystyrene LHC0007 Cytokine Human 25-Plex Panel Eotaxin, GM-CSF, IFN-, IFN-, IL-1, IL-1ra, IL-2, IL-2R, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40/p70), IL-13, IL-15, IL-17, IP-10, MCP-1, MIG, MIP-1, MIP- 1, RANTES, TNF- Polystyrene LHC0009 Human Chemokine II 5-Plex Panel ENA-78, I-309/CCL1, MDC/CCL22, MIP-3 /CCL20, TARC/CCL17 Polystyrene LHC0012 Chemokine Human 10-Plex Panel Eotaxin, GRO-, IP-10, MCP-1, MCP-2, MCP-3, MIG, MIP-1, MIP-1, RANTES Polystyrene LHC6001 Matrix Metalloprotein Human 3-Plex MMP-3, MMP-9, MMP-13 Polystyrene LHC6002 Panel Cytokine Human 30-Plex Panel EGF, eotaxin, FGF basic, G-CSF, GM-CSF, HGF, IFN-, IFN-, IL-1, IL-1RA, IL- 2, IL-2R, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40/p70), IL-13, IL-15, IL-17, IP-10, MCP-1, MIG, MIP-1, MIP-1, RANTES, TNF-, VEGF Polystyrene LHC6003 Cytokine Ultrasensitive Human 10-Plex Panel GM-CSF, IFN-, IL-1, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, TNF- Polystyrene LHC6004 Acute Phase 4-Plex Panel 2 microglobulin, haptoglobin, CRP, Gc globulin Polystyrene LHC6006 Apoptosis 3-Plex Panel Cytochrome c, caspase-3 [175/176], PARP [214/215] Polystyrene LHO0007 Akt Pathway Phospho Magnetic 7-Plex Panel Akt [ps473], GSK-3 [ps9], IRS-1 [ps312], IGF-1R [pypy1135/1136], IR [pypy1162/1163], p70s6k [ptps421/424], PRAS40 [pt246] Magnetic LHO0001M Akt Pathway Total Magnetic 7-Plex Panel Akt, GSK-3, IGF-1R, IR, IRS-1, p70s6k, PRAS40 Magnetic LHO0002M Akt Pathway Phospho 7-Plex Panel Akt [ps473], GSK-3 [ps9], IRS-1 [ps312], IGF-1R [pypy1135/1136], IR Polystyrene LHO0001 [pypy1162/1163], p70s6k [ptps421/424], PRAS40 [pt246] Akt Pathway Total 7-Plex Panel Akt, GSK-3, IGF-1R, IR, IRS-1, p70s6k, PRAS40 Polystyrene LHO0002 Monkey Cytokine 29-Plex Magnetic Panel NEW EGF, eotaxin, FGF basic, G-CSF, GM-CSF, HGF, IFN-, IL-1, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-15, IL-17, IP-10, I-TAC, MCP-1, MDC, MIF, MIG, MIP-1, MIP-1, RANTES, TNF-, VEGF Magnetic LPC0005M Th1/Th2 Cytokine Monkey 5-Plex Panel IFN-, IL-2, IL-4, IL-10, TNF- Polystyrene LPC0001 Chemokine Monkey 5-Plex Panel IL-8, MCP-1, MIP-1, MIP-1, RANTES Polystyrene LPC0002 Cytokine Mouse Magnetic 10-Plex Panel GM-CSF, IFN-, IL-1, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p40/p70), TNF- Magnetic LMC0001M Inflammatory Cytokine Mouse Magnetic 4-Plex Panel GM-CSF, IL-1, IL-6, TNF- Magnetic LMC0003M Cytokine Mouse Magnetic 20-Plex Panel FGF basic, GM-CSF, IFN-, IL-1, IL-1, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, IL-12 (p40/p70), IL-17, IP-10, KC, MCP-1, MIG, MIP-1, TNF-, VEGF Magnetic LMC0006M Cytokine Mouse 10-Plex Panel GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p40/p70), TNF-α Polystyrene LMC0001 Life Technologies Multiplex assays: multiplex kits 19

20 Th1/Th2 Cytokine Mouse 6-Plex Panel IFN-, IL-2, IL-4, IL-5, IL-10, IL-12 (p40/p70) Polystyrene LMC0002 Inflammatory Cytokine Mouse 4-Plex GM-CSF, IL-1, IL-6, TNF- Polystyrene LMC0003 Panel Chemokine Mouse 5-Plex Panel IP-10, KC, MCP-1, MIG, MIP-1 Polystyrene LMC0005 Cytokine Mouse 20-Plex Panel FGF basic, GM-CSF, IFN-, IL-1, IL-1, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, IL-12 (p40/p70), IL-17, IP-10, KC, MCP-1, MIG, MIP-1, TNF-, VEGF Polystyrene LMC0006 Cytokine Rat Magnetic 10-Plex Panel NEW GM-CSF, IL-1, IFN-, IL-1, IL-2, IL-4, IL-6, IL-10, IL-12 (p40/p70), TNF- Magnetic LRC0002M Cytokine Rat 10-Plex Panel GM-CSF, IL-1, IFN-, IL-1, IL-2, IL-4, IL-6, IL-10, IL-12 (p40/p70), TNF- Polystyrene LRC0002 Swine Cytokine 7-Plex Magnetic Panel NEW IL-1, IL-4, IL-8, IL-10, IFN-, IFN-, TNF- Magnetic LSC0001M TEL FAX TEL FAX FAX TEL FAX TEL FAX TEL FAX TEL FAX The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. 2013, Life Technologies Japan Ltd. All rights reserved. Printed in Japan. PA008-A1310OB