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1 J. Cell Biol.,,. Segev, N.Curr. Opin. Cell Biol.,,. Lafont, F., Lecat, S., Verkade, P., & Simons, K.J. Cell Biol.,,. Mostov, K.E., Verges, M., & Altschuler, Y.Curr. Opin. Cell Biol.,,. Sato, T., Mushiake, S., Kato, Y., Sato, K., Sato, M., Takeda, N., Ozono, K., Miki, K., Kubo, Y., Tsuji, A., Harada, R., & Harada, A.Nature,,. Huber, L.A., Pimplikar, S., Parton, R.G., Virta, H., Zerial, M., & Simons, K.J. Cell Biol.,,. Huber, L.A., de Hoop, M.J., Dupree, P., Zerial, M., Simons, K., & Dotti, C.J. Cell Biol.,,. Harada, A., Oguchi, K., Okabe, S., Kuno, J., Terada, S., Ohshima, T., Sato-Yoshitake, R., Takei, Y., Noda, T., & Hirokawa, N.Nature,,. Pecache, N., Patole, S., Hagan, R., Hill, D., Charles, A., & Papadimitriou, J.M.Postgrad Med. J.,,. Analyses of molecules involved in intracellular polarized transport Akihiro Harada Laboratory of Molecular Traffic, Department of Molecular and Cellullar Biology, Institute for Molecular and Cellular Regulation, Gunma University, Showa, Maebashi, Gunma, Japan KaiC KaiC
2 Synechococcus elongatus PCC kaia kaibc kaibc kaia kaib kaic kaibc KaiA KaiC KaiC KaiC KaiC KaiA KaiC KaiB KaiA KaiC N CI C CII ATPase WalkerA B motif X KaiC ATP CII S T ATP γ-oh CII KaiC E Q KaiC E Q KaiC Ala KaiC KaiA KaiB KaiC ATP
3 Q KaiC KaiC in vitro KaiC KaiC SDS-PAGE A S T T S pt ps pt S ps T Bγ- P ATP S pt ps pt ps T A T S T S KaiC KaiC Q T v T v Q v v T T Q KaiC KaiC S T S KaiC S KaiC S C S O Shea KaiC Kai KaiC Kai SKai KaiA KaiB KaiC in vivo in vitro KaiC Kai KaiC KaiB S KaiC KaiA-KaiC KaiB KaiB KaiC A B γ- P ATP KaiC kda B KaiA KaiC KaiC KaiB-KaiC
4 S T KaiC KaiC in vitro γ- P ATP SDS- PAGE CBB S T T S pt ps pt S ps T S pt ps pt ps T CBB KaiC S KaiC KaiA KaiA Kai KaiC KaiC X Kai Kai KaiC KaiC ATPase ATP KaiC KaiC ATPase ATP ATPase KaiC ATPase Q
5 Kai KaiC FLAG KaiC KaiA KaiB FLAG KaiC-AT S A KaiC-DT S D KaiC-AA S A; T A KaiC-DE S D; T E KaiB S KaiC KaiB KaiA-KaiC KaiB KaiC KaiC Kai KaiC γ- P ATP SDS-PAGE nm kda KaiC kda ATPase S T KaiC ATPase ATPase ATPase GS SORST Young, M.-W. & Kay, S.-A.Nat. Rev. Genet.,,. Bell-Pedersen, D., Cassone, V.-M., Earnest, D.-J., Golden, S.- S., Hardin, P.-E., Thomas, T.-L., & Zoran, M.-J.Nat. Rev. Genet.,,. Nakajima, M., Imai, K., Ito, H., Nishiwaki, T., Murayama, Y., Iwasaki, H., Oyama, T., & Kondo, T.Science,,. Ishiura, M., Kutsuna, S., Aoki, S., Iwasaki, H., Andersson, C.-
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R., Tanabe, A., Golden, S.-S., Johnson, C.-H., & Kondo, T. Science,,. Nishiwaki, T., Iwasaki, H., Ishiura, M., & Kondo, T. Proc. Natl. Acad. Sci. USA,,. Xu, Y., Mori, T., & Johnson, C.-H.EMBO J.,,. Iwasaki,
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A S1 9 24 14 00 17 00 Epigenomic regulation of cell fate determination and homeostasis Organizers: (National Institute of GeneticsKyushu University 14 00 S1-1 1 RIKEN BioResource Center 2 Graduate School
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pp. D- D-DAO DAO D- D- D- D- o- DAO-D- D-DAO DAO Hans Krebs FAD X DAO DAO DAO D- Structure and reaction mechanism of D-amino acid oxidase Yasuzo Nishina School of Health Sciences, Kumamoto University,
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Structure and function of nitric oxide synthases 3) Furchgott, R. F., Zawadzki, J. V. : Nature, 288,373-376 (1980) 4) Palmer, R. M., Ferrige, A. G., Moncada, S.: Nature, 327,524-5260987) 5) Alderton,
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インターロイキン インターロイキンは リンパ球や単球 マクロファージなどの免疫反応への寄与が知られる白血球から産生されることから 細胞間 ( inter-) 白血球 ( leukocyte -leukin) より Interleukin と名づけられました 現在までに 30 種類以上のインターロイキンが同定され 発見された順番に番号が付与されており 中にはケモカイン ( IL-8) やインターフェロン
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500μm Before ATP After 10 Sikora J, Orlov SN, Furuya K, and Grygorczyk R (2015) Hemolysis is a primary and physiologically relevant ATP release mechanism in human. Blood 125: 1845 (Response to a letter)
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