生体内に存在する蛋白質の中には、20種類の基本アミノ酸だけでは機能の発現ができない蛋白質が存在する
|
|
|
- ともみ すわ
- 5 years ago
- Views:
Transcription
1 21 Vol.11 No.1 Linda Rasubala Centre National de la Recherche Scientifique Dominique Fourmy Molecular Basis for the selenocysteine incorporation Toyoyuki Ose 1, Linda Rasubala 1, Satoko Yoshizawa 2, Dominique Fourmy 2, Daisuke Kohda 1, Katsumi Maenaka 1 1 Medical Institute of Bioregulation, Kyushu University 2 Centre National de la Recherche Scientifique Selenium, an essential trace element, is found in antioxidant proteins in the form of selenocysteine, the 21 st amino acid. In bacteria, the selenocysteine incorporation into proteins requires the elongation factor SelB that has the unusual property to bind directly to both transfer RNA (trna) and messenger RNA (mrna). The N-terminal domain of SelB is homologous to elongation factor Tu (EF-Tu) that transfers aminoacyl-trnas (aa-trnas) to the ribosomal A site in the form of a ternary complex EF-Tu-GTP-aa-tRNA. The accuracy of mrna decoding depends on base-pairings between the codon on the mrna and the anticodon on trna followed by EF-Tu GTP hydrolysis as well as conformational changes in the ribosome. In the case of the selenocysteine incorporation into polypeptides, a UGA codon is recoded as a Sec codon in the presence of the selenocysteine insertion sequence (SECIS) at its downstream. The unique extra C-terminal domain of SelB recognizes the SECIS mrna hairpin to deliver the selenocysteyl-trna (Sec-tRNA Sec ) at a UGA codon. However, the molecular basis of high specific recognition of SelB to the SECIS mrna hairpin has been unknown. We have reported the 2.3Å resolution crystal structure of the C-terminal mrna-binding domain of Moorella thermoacetica SelB complexed with SECIS RNA hairpin. This is the first structure of a complex between an RNA and a winged-helix domain. The winged-helix domain is famous for DNA-binding motif, recently implicated in RNA binding. The structure illustrates the specificity of binding and reveals a new mode of RNA recognition. A striking feature of the complex is a 70 angle between the RNA helical axis and the protein that allows the complex to wrap around the small ribosomal subunit. This geometry explains how SelB can bind to both mrna and trna whose sites are distant on the ribosome mrna UGA -1-
2 構造生物 Vol.11 No 年 6 月発行 般的に知られる翻訳終結反応では mrna 上に UGA などの終止コドンが現れると そ れに対応してリボソーム上の A-site にはアミノアシル trna ではなく release factor (RF)が取り込まれる RF は P-site の trna とペプチド鎖の間のエステル結合を加水分 解し 翻訳の終了したペプチド鎖が放出される しかし mrna 上の UGA コドンの 下流に SECIS と呼ばれる特殊な配列が存在した場合には 状況が一変する 下流に SECIS を持った mrna の UGA コドンが A-site に取り込まれると UGA は終止コド ンとして機能せずにセレノシステインを指定するコドンへと変身する A-site には RF が取り込まれないのでペプチド鎖はリボソームから解離せずに新たにセレノシステ インを付加して 伸長を続ける 図 1 SelB-SECIS 及びリボソームの相互作用 この現象を図 1 に示 した SECIS配列を有す るmRNAは SelBのC末 端 ド メ イ ン が SECIS RNA領域を認識するた め SelBを結合した状態 でリボソーム小サブユ ニットに引き込まれて い く mrna 上 の SelB がリボソームに到達し たとき SelBのN末端ド 図 2 Moorella thermoacetica 由来 SelB 分子のドメイン構造 メインに結合したセレ ノ シ ス テ イ ル trnasec がA-siteに入り アンチコドン部分がmRNAのUGAコドン部分と相互作用することに より UGAコドンがセレノシステインとして翻訳される つまりSelBはmRNAおよび trnaに結合することのできる多機能な伸長因子であると言える 図 2 は酢酸生産菌 Moorella thermoacetica由来 SelB分子のドメイン構造を表したものである バクテリア 真正細菌 のSelBは図 2 のようにN末端ドメインおよびC末端ドメインの二つのド メインに大別することができる N末端ドメイン(SelB-N)はEF-Tuと相同性のあるアミ ノ 酸 配 列 を 有 す る た め GTP 加 水 分 解 活 性 を 保 持 し セ レ ノ シ ス テ イ ン trnasec(sec-trnasec)を結合することができる 一方で SECISの特徴的な構造を認識 し 結合することできるのはSelBのC末端ドメイン(SelB-C)である -2-
3 NMR X RNA M. thermoacetica SECIS-RNA NMR 1 F(fdhF) mrna SECISmimic RNA 3 SECIS SelB-C SECIS-RNA 1, 2 M. thermoacetica SelB-C ( ) Selmer 3 SelB-C DNA winged helix(wh) 3 fdhf(a) M. thermoacetica(b) SECIS ( 2) SelB N (SelB-N) EF-Tu trna Sec-tRNA Sec SelB SECIS trna A-site SECIS SECIS-RNA SelB-C SECIS 4 ( :SelB-M) RNA SelB SelB-C K d =1µM SECIS-RNA Moorella thermoacetica SelB-M( ) DNA pgex-2t(amersham Biosciences) N GST glutathione agarose column(sigma) -3-
4 thrombin(sigma) glutathione agarose CM_Sepharose 48 RNA SECIS RNA M. thermoacetica FdhF SECIS-mRNA 23 (5 -GGCGUUGCCGGUCUGGCAACGCC-3 ) 3 GC SECIS RNA 10mM Tris-HCl ph7.0, 100mM NaCl, 0.1mM EDTA 8mg ml M Na-HEPES ph7.5, 1.4M trisodium citrate dihydrate 5 ethylene glycol 15% 2-2. P (a=71.69 Å, b=81.69å, c=169.58å ) V M 3 4 -RNA M. thermoacetica SelB-M FdhF-SECIS AMoRe 6 Molrep 7 AMoRe SelB-M Molep SECIS SelB-SECIS -RNA 2.3Å CNS 8 R/R free factor = 0.224/ A B RNA RNA RNA DNA DNA- RNA AB C ( ) RNA C AB D RNA -4-
5 4 A, B, C D 2-4. SelB winged helix(wh) RNA SelB-C WH L SECIS-RNA C WH (WH4) ( 5) 5 SelB-M WH(SelB-C WH) helix-turn-helix WH DNA WH DNA RNA WH WH RNA WH -DNA WH 3 (H3: ) DNA major groove minor groove -5-
6 SelB WH H3 N RNA RNA H2 S2-S3 wing(w1)rna SelB-M SelB 452Å 2 buried-surface H3 groove buried-surface Sap1-DNA 818Å 2 buried-surface 9 452Å 2 buried-surface K d =1µM 2-5. SelB-M RNA SelB-C Cα rmsd 0.55Å SECIS SECIS NMR 4 G22-C25 ( 2) SECIS-RNA SelB SECIS-RNA 6a, b G23 U26 U26 6 SECIS-RNA (a, ) SECIS-SelB (b) -6-
7 6a G23 2 U24 O5 U24 2 C25 6b G23 U24 G23 U24 UGA SECIS M. thermoacetica SelB-SECIS SECIS WHH3 W1 SelB-WH4 H3 W1 G23 ( 5) G23 G23 Leu595 Leu610 Arg624 W1 Asp627 Arg624 G23 ( 7) G23 (O6) Arg629 G23 Glu614 G23 RNA 1, 2 Glu614 Arg629 7 SECIS SelB ( ) -7-
8 RNA Arg599, Ser605, Arg606 Lys607 ( 8) Arg606 RNA Arg606 U24 (O4) U24 1, 2 8 SECIS SelB ( ) 4. SelB-SECIS 1998 mrna 70S 10 9a, b 30S Head Body rrna h16 h33 SelB-C L 9c,d 30S SelB-C-CECIS SelB-M SECIS SelB-C SelB-C SECIS-RNA SelB-C WH3, WH4 RNA 70 SelB-C L SECIS-RNA SelB 30S SECIS 5 30S mrna 9a, b, c SECIS-RNA 30S mrna RNA AUG SECIS-RNA G23 23 upper stem 3b 12 -mrna A mrna 12-8-
9 EF-Tu 30S X EF-Tu-tRNA 11 EF-Tu-tRNA-30S 12 30S EF-Tu C SelB-C N 9a, b SelB-N EF-Tu SelB 30S SelB N Sec-tRNA Sec upper stem L SelB-C WH (WH4) UGA trna Sec 30S h16 h33 mrna-selb-sec-trna Sec -mrna ( 9a, b) SelB SECIS - GTP 30S head shoulder 13 SelB-mRNA SelB mrna SECIS mrna 30S SECIS SelB 9e SelB-SECIS (A, B, C) C RNA AB SECIS G23 C SelB-SICIS 5. SelB C (SelB-M) mrna SECIS-RNA SelB-M DNA winged helix SECIS PF SPring8-9-
10 1. Fourmy, D., Guittet, E. & Yoshizawa, S. Structure of prokaryotic SECIS mrna hairpin and its interaction with elongation factor SelB. Journal of Molecular Biology 324, (2002). 2. Kromayer, M., Wilting, R., Tormay, P. & Böck, A. Domain structure of the prokaryotic selenocysteine-specific elongation factor SelB. Journal of Molecular Biology 262, (1996). 3. Selmer, M. & Su, X. D. Crystal structure of an mrna-binding fragment of Moorella thermoacetica elongation factor SelB. The Embo Journal 21, (2002). 4. Yoshizawa, S. et al. Structural basis for mrna recognition by elongation factor SelB. Nat Struct Mol Biol 12, (2005). 5. Rasubala, L. et al. Crystallization and preliminary X-ray analysis of the mrna-binding domain of elongation factor selb in complex with RNA. Acta Crystallogr. F61, (2005). 6. Navaza, J. AMoRe: an automated package for molecular replacement. Acta Crystallogr. A50, (1994). 7. Vagin, A. A. & Isupov, M. N. Spherically averaged phased translation function and its application to the search for molecules and fragments in electron-density maps. Acta Crystallogr D Biol Crystallogr 57, (2001). 8. Brunger, A. T. et al. Crystallography & NMR system: A new software suite for macromolecualr structure determination. Acta Crystallographica D Biol Crystallogr. 54, (1998). 9. Mo, Y., Vaessen, B., Johnston, K. & Marmorstein, R. Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins. Mol Cell 2, (1998). 10. Yusupova, G. Z., Yusupov, M. M., Cate, J. H. & Noller, H. F. The path of messenger RNA through the ribosome. Cell 106, (2001). 11. Nissen, P. et al. Crystal structure of the ternary complex of Phe-tRNAPhe, EF-Tu, and a GTP analog. Science 270, (1995). 12. Stark, H. et al. Visualization of elongation factor Tu on the Escherichia coli ribosome. Nature 389, (1997). 13. Ogle, J. M., Murphy, F. V., Tarry, M. J. & Ramakrishnan, V. Selection of trna by the ribosome requires a transition from an open to a closed form. Cell 111, (2002). -10-
ナノの技術をバイオに応用
本日まで お試し期間 なので 出席は取りません 現代生物学概論 2 遺伝子 ( プログラム ) と蛋白質 ( ナノマシン ) 先進理工学科 化学生物学研究室 准教授 生体機能システムコース 瀧真清 1 本日の概要 : 蛋白質生合成の全スキーム D から蛋白質への情報の流れ アミノ酸から蛋白質への物質の流れ 転写 D 本日は詳細は省略 アミノアシル tr 合成酵素 (RS) 翻訳 mr コドンーアンチコドンの対合
1_alignment.ppt
" " " " n " n n " n " n n n " n n n n " n LGPSSKQTGKGW-SRIWDN! + +! LN-ITKSAGKGAIMRLGDA! " n -------TGKG--------!! -------AGKG--------! " n w w w " n w w " " " 11 12 " n w w w " n w w A! M! O! A!
会報35号表紙.pdf
35 No. (2017 1 ) RNA 2017 2017 TV 365 365 chunky 26 RNA 2017 Congratulations26 30-1 - RNA 2017-2 - - 3 - - 4 - - 5 - - 6 - 2016 8 31 9 2 RNA 2016 1 RNA RNA RNA RNA RNA 1-7 - RNA RNA 3 3 7 31 RNA ncrna
25 6 7 X (crystallizing ligand) Fv Fv 3.5 Co-crystallization of membrane proteins with antibody fragments may emerge as a general tool to facilitate crystal growth and improve crystal quality. The bound
宇宙環境利用蛋白質結晶生成応用利用実証プロジェクトのご案内
16 8 23 1. 14 17 ISS JEM (JAXA) 2. JAXA 14 6 1 4 1 02/02/2003 29/08/2003 29/01/2004 11/08/2004 Baikonur Kazakhstan Progress 03/05/2003 28/10/2003 29/04/2004 19/10/2004 Kazakhstan Soyuz 13 weeks 9 weeks
The Phase Behavior of Monooleoylglycerol-Water Systems Mivoshi Oil & Fat Co.. Ltd. Faculty of Science and Technology, Science University of Tokyo Inst
The Phase Behavior of Monooleoylglycerol-Water Systems Mivoshi Oil & Fat Co.. Ltd. Faculty of Science and Technology, Science University of Tokyo Institute of Colloid and Interface Science, Science University
Microsoft PowerPoint - プレゼンテーション1
A A RNA からタンパク質へ mrna の塩基配列は 遺伝暗号を介してタンパク質のアミノ酸の配列へと翻訳される trna とアミノ酸の結合 RNA 分子は 3 通りの読み枠で翻訳できる trnaは アミノ酸とコドンを結びつけるアダプター分子である (Ψ; プソイドウリジン D; ジヒドロウリジンどちらもウラシルが化学修飾したもの ) アミノアシル trna 合成酵素によって アミノ酸と trna
Fig. 3 Flow diagram of image processing. Black rectangle in the photo indicates the processing area (128 x 32 pixels).
Fig. 1 The scheme of glottal area as a function of time Fig. 3 Flow diagram of image processing. Black rectangle in the photo indicates the processing area (128 x 32 pixels). Fig, 4 Parametric representation
Core Ethics Vol. Epstein, CI CI CI CI CI CI CI CI Epstein, CI CI CI CI CI CI CI CI CI CI Schindler, CI CI CI CI NIH CI Finn FDA / M CI N CI N / M, CI
Core Ethics Vol. Cochlear Implant CI,, CI CI,,, CI CI CI CI CI, CI CI CI CI CI CI CI CI CI CI CI CI CI Core Ethics Vol. Epstein, CI CI CI CI CI CI CI CI Epstein, CI CI CI CI CI CI CI CI CI CI Schindler,
昭和恐慌期における長野県下農業･農村と産業組合の展開過程
No. 3, 169-180 (2002) The Family in Modern Japan: its Past, Present and Future An Essay at Restoring Love as the Basis of Family Ties YAMANE Naoko Nihon University, Graduate School of Social and Cultural
表1.eps
Vol.1 C ontents 1 2 cell-free EMBO J Proc. Natl. Acad. Sci. USA NatureEMBO J 3 RNA NatureEMBO J Nature EMBO J 4 5 HCV HCV HCV HCV RNA HCV in situ 6 7 8 Nat Struct Mol Biol J Biol Chem Nat Commun J Virol
IR0036_62-3.indb
62 3 2016 253 272 1921 25 : 27 8 19 : 28 6 3 1921 25 1921 25 1952 27 1954 291960 35 1921 25 Ⅰ 0 5 1 5 10 14 21 25 34 36 59 61 6 8 9 11 12 16 1921 25 4 8 1 5 254 62 3 2016 1 1938.8 1926 30 1938.6.23 1939.9
NINJAL Project Review Vol.3 No.3
NINJAL Project Review Vol.3 No.3 pp.107 116 March 2013 Learners Spoken Corpus of Japanese and Developmental Sequence of Verbs SAKODA Kumiko 1 C-JAS 2 2.1 1 1 8 13 3 OPI Oral Proficiency Interview 9 10
303 Human Factors in Nuclear Power Plant with Focus on Application of Man-machine Interface and Advanced Data Processing Technologies to Nuclear Power Industry Abstract We discuss issues involved in a
% 1% SEM-EDX - X Si Ca SEM-EDX SIMS ppm % M M T 100 % 100 % Ba 1 % 91 % 9 % 9 % 1 % 87 % 13 % 13 % 1 % 64 % 36 % 36 % 1 % 34 46
Review on Hair Analysis in the Wakayama Arsenic Case Jun KAWAI Department of Materials Science and Engineering, Kyoto University Sakyo-ku, Kyoto 606-8501, Japan Received 6 December 2014, Revised 29 December
A Higher Weissenberg Number Analysis of Die-swell Flow of Viscoelastic Fluids Using a Decoupled Finite Element Method Iwata, Shuichi * 1/Aragaki, Tsut
A Higher Weissenberg Number Analysis of Die-swell Flow of Viscoelastic Fluids Using a Decoupled Finite Element Method Iwata, Shuichi * 1/Aragaki, Tsutomu * 1/Mori, Hideki * 1 Ishikawa, Satoshi * 1/Shin,
Title 社 会 化 教 育 における 公 民 的 資 質 : 法 教 育 における 憲 法 的 価 値 原 理 ( fulltext ) Author(s) 中 平, 一 義 Citation 学 校 教 育 学 研 究 論 集 (21): 113-126 Issue Date 2010-03 URL http://hdl.handle.net/2309/107543 Publisher 東 京
会報36号表紙
36 No. ( 2017 9 ) RNA 2017 RNA 19 7 19 21 7 8 JR RNA RNA 20 RNA RNA RNA RNA 10 50 13 26% 40 RNA RNA-SEQRNAiCRISPRCLIP RNA RNA - 1 - 3 29 8-2 - ips - 3 - - 4 - - 5 - RNA 29 RNA RNA 5 microrna trna? RNA
k = The Last Samurai Tom Cruise [1] Oracle Ken Watanabe (I) has a Bacon number of 2. 1: 6(k 6) (small world p
![k = The Last Samurai Tom Cruise [1] Oracle Ken Watanabe (I) has a Bacon number of 2. 1: 6(k 6) (small world p](/thumbs/91/107565868.jpg "k = The Last Samurai Tom Cruise [1] Oracle Ken Watanabe (I) has a Bacon number of 2. 1: 6(k 6) (small world p")
The size of the world It is a small world Araseki Hitoshi Can you believe that everyone is at most six steps away from any other person on the Earth? This phenomenon, which is called small world phenomenon,
ス H95) は EF-G の GTP 結合部位と明確に相互作用しており このループが GTP 加水分解に直接関与していることが示唆されました ( 図 4 右 ) 本研究成果は わが国で推進している タンパク 3000 プロジェクト の一環として行われたものです 本研究成果の詳細は 米国の学術雑誌
報道発表資料 2007 年 3 月 10 日 独立行政法人理化学研究所 ドイツシャリティ ベルリン医科大学 タンパク質生合成工場 リボソーム との相互作用による翻訳伸長因子の活性化メカニズムを解明 - 遺伝子翻訳の重要な過程をスナップショットで可視化 - ポイント T. thermophilus には EF-G の遺伝子が 2 種類あることが判明した 全生物に共通な翻訳伸長因子 EF -G の GTP
Perl + α. : DNA, mrna,,
2009 Perl + α. : DNA, mrna,, DNA .. DNA A C G T DNA 2 A-T, C-G DNA NH 2 NH 2 O - O O N P O - O CH 2 O N N O - O P O CH 2 O N O - O O P O NH 2 O - O - N CH 2 O N O OH OH OH DNA or RNA (U) (A) (G) (C)
<95AA8E718CA4838C835E815B D862E696E6464>
T1r T1r1 T1r3 T1r2 T1r3 [1] T1r T1r 500 LBD 1 LBD T1rLBD [2] T1r T1r2a T1r3 LBD [3] T1r2a/T1r3 L- [4] T1r2a/T1r3LBD M~ M T1rLBD FRET FRET T1rLBD [3] 1 T1r 2 T1r2a/T1r3 X [3] 28 75 March 2017 T1rLBD imsaxst1r2a/t1r3lbd
年次大会原稿最終.PDF
Structural Analysis of Coating Layer Containing Plastic Pigments Yoko Saito, Hideki Touda, Junji Kasai ZEON Corporation Hitomi Hamada, Toshiharu Enomae, Fumihiko Onabe The University of Tokyo Recently,
untitled
ORYZA POLYAMINE ORYZA POLYAMINE -P -PC -LC 1. Ver.1.0 MM ver. 1.0MM 1) Roseeuw D.I. et al., Epidermal keratinocytes actively maintain their intracellular polyamine levels. Cell Tissue Kinet. 16, 493-504
A b a B AaBb Ab ab 1 1 AB ab A-b a-b 2.Meiosis recombination Meiosis mitosis crossover recombination bivalent DNA 2
遺 伝 と 遺 伝 子 2007 年 度 参 考 問 題 リストの 解 答 例 xxax2002@yahoo.co.jp 2007 50 50 1. Mendel s second law of inheritance 2006 2006 A B A B Mendel s second law of inheritance principle of independent segregation P
Core Ethics Vol. -
Core Ethics Vol. - Core Ethics Vol. - AV LSSLSS, CIL CIL, Core Ethics Vol. , Core Ethics Vol.,, K ALS Core Ethics Vol. emotional labor Hochschild : Core Ethics Vol. QOL ALS - / ALS ALS - vol.. vol. :
*.E....... 139--161 (..).R
A Preliminary Study of Internationalization at the Local Level: The Case of Aikawa Town in Kanagawa Prefecture, Japan FUKUSHIMA Tomoko and FUJISHIRO Masahito In recent years, as foreign residents increase
本文３／ＹＡ８１８３Ｅ
pp. N Tom Tom Tom - Tom µm Soft interaction for the recognition of mitochondrial targeting signal Daisuke Kohda Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi,
An Empirical Study on Media Frames: How the Newspapers Covered "All Five Walks" in the Summer High School Baseball Tournament in 1992. IBARAGI Yoshiko Recently the role of "media frames" in the process
untitled
JAIS 1 2 1 2 In this paper, we focus on the pauses that partly characterize the utterances of simultaneous interpreters, and attempt to analyze the results of experiments conducted using human subjects
06_学術_関節単純X線画像における_1c_梅木様.indd
Arts and Sciences X The formulation of femoral heard measurement corrected enlargement ratio using hip joints X-ray Imaging 1 2 1 1 1 2 Key words: Bipolar Hip Arthroplasty (BHA) Preoperative planning Enlargement
研究成果報告書
IgE Can f 1 Can f 2 X -sheet (Can f 3) IgE Human-animal bonds become stronger. Dogs as behalf of pets are tightly associated with human living life. Due to the intimate relationship, human may be sensitized
( ) ( ) (action chain) (Langacker 1991) ( 1993: 46) (x y ) x y LCS (2) [x ACT-ON y] CAUSE [BECOME [y BE BROKEN]] (1999: 215) (1) (1) (3) a. * b. * (4)
![( ) ( ) (action chain) (Langacker 1991) ( 1993: 46) (x y ) x y LCS (2) [x ACT-ON y] CAUSE [BECOME [y BE BROKEN]] (1999: 215) (1) (1) (3) a. * b. * (4)](/thumbs/40/20534161.jpg "( ) ( ) (action chain) (Langacker 1991) ( 1993: 46) (x y ) x y LCS (2) [x ACT-ON y] CAUSE [BECOME [y BE BROKEN]] (1999: 215) (1) (1) (3) a. * b. * (4)")
1 1 (lexical conceptual structure, LCS) 2 LCS 3 4 LCS 5 6 2 LCS (1999) LCS 2 (1) [x ACT(-ON y)] CAUSE [BECOME [z BE-AT w]] 1 (1993) ( ) V1 V2 2 (1) y z y z (5.3 ) ( ) ( ) (action chain) (Langacker 1991)
Safety Performance of Steel Deck Plate (Flat Decks) Used for Concrete Slab Moulding CONTENTS 1. Introduction ---------------------------------------------------------------- (2) 2. Flat Decks ------------------------------------------------------------------
1., 1 COOKPAD 2, Web.,,,,,,.,, [1]., 5.,, [2].,,.,.,, 5, [3].,,,.,, [4], 33,.,,.,,.. 2.,, 3.., 4., 5., ,. 1.,,., 2.,. 1,,
![1., 1 COOKPAD 2, Web.,,,,,,.,, [1]., 5.,, [2].,,.,.,, 5, [3].,,,.,, [4], 33,.,,.,,.. 2.,, 3.., 4., 5., ,. 1.,,., 2.,. 1,,](/thumbs/50/25936361.jpg "1., 1 COOKPAD 2, Web.,,,,,,.,, [1]., 5.,, [2].,,.,.,, 5, [3].,,,.,, [4], 33,.,,.,,.. 2.,, 3.., 4., 5., ,. 1.,,., 2.,. 1,,")
THE INSTITUTE OF ELECTRONICS, INFORMATION AND COMMUNICATION ENGINEERS TECHNICAL REPORT OF IEICE.,, 464 8601 470 0393 101 464 8601 E-mail: matsunagah@murase.m.is.nagoya-u.ac.jp, {ide,murase,hirayama}@is.nagoya-u.ac.jp,
パナソニック技報
Panasonic Technical Journal Vol. 64 No. 2 Nov. 2018 Optical Disc Archiving System with 100 Years Lifespan of Digital Data Takuto Yamazaki Yasushi Kobayashi Blu-ray Disc 1 Archival Disc 2 3300 GB 10012
Present Situation and Problems on Aseismic Design of Pile Foundation By H. Hokugo, F. Ohsugi, A. Omika, S. Nomura, Y. Fukuda Concrete Journal, Vol. 29
Present Situation and Problems on Aseismic Design of Pile Foundation By H. Hokugo, F. Ohsugi, A. Omika, S. Nomura, Y. Fukuda Concrete Journal, Vol. 29, No. 8, pp. 4-12, Aug. 1986 Synopsis The pile foundation
システム開発プロセスへのデザイン技術適用の取組み～HCDからUXデザインへ～
HCDUX Approach of Applying Design Technology to System Development Process: From HCD to UX Design 善方日出夫 小川俊雄 あらまし HCDHuman Centered Design SE SDEMHCDUIUser Interface RIARich Internet ApplicationUXUser
01-加藤 実-5.02
Bull. Natl. Mus. Nat. Sci., Ser. E, 30, pp. 1 13, December 21, 2007 1 2 3 1 169 0073 3 23 1 2 523 0058 961 3 248 0036 3 5 6 The Mechanism of the Automatic Wari-koma Dial in the Japanese Clocks and its
資源と素材
(Shigen to Sozai) Vol.116 p. 285 290 (2000) 共存硫酸塩を含む強酸性 MnSO 4 溶液中のオゾン酸化によって生成した沈殿の X 線回折および放電特性 * 西村忠久 1 梅津良昭 2 X-ray Diffraction and Discharge Behavior of Precipitates Produced by Ozone Oxidation in
1 2 1 2012 39 1964 1997 1 p. 65 1 88 2 1 2 2 1 2 5 3 2 1 89 1 2012 Frantzen & Magnan 2005 2010 6 N2 2014 3 3.1 2015 2009 1 2 3 2 90 2 3 2 B1 B1 1 2 1 2 1 2 1 3.2 1 2014 2015 2 2 2014 2015 9 4.1 91 1 2
Instability of Aerostatic Journal Bearings with Porous Floating Bush at High Speeds Masaaki MIYATAKE *4, Shigeka YOSHIMOTO, Tomoaki CHIBA and Akira CH
Instability of Aerostatic Journal Bearings with Porous Floating Bush at High Speeds Masaaki MIYATAKE *4, Shigeka YOSHIMOTO, Tomoaki CHIBA and Akira CHIBA Department of Mechanical Engineering, Tokyo University
研究成果報告書(基金分)
The most remarkable characteristic of the mammalian neocortex is its layered structure. Because all, but only the mammalian species, have this layered structure in the telencephalon, it has been accepted
能書単頁9[1].5(2)
![能書単頁9[1].5(2)](/thumbs/87/96620151.jpg "能書単頁9[1].5(2)")
ips CytoTune -ips ver.1.0 1 I CytoTune ips 3 II 3 3 4 5 III CytoTune ips ips 6 6 6 6 7 1) 2) ips 8 ips 8 SeV 9 SeV 10 IV Q&A 11 V 12 12 VI...13 13...13 2 I CytoTune ips CytoTune ips 4 OCT3/4SOX2 KLF4c
ABSTRACT The Social Function of Boys' Secondary Schools in Modern Japan: From the Perspectives of Repeating and Withdrawal TERASAKI, Satomi (Graduate School, Ochanomizu University) 1-4-29-13-212, Miyamaedaira,
1 Fig. 1 Extraction of motion,.,,, 4,,, 3., 1, 2. 2.,. CHLAC,. 2.1,. (256 ).,., CHLAC. CHLAC, HLAC. 2.3 (HLAC ) r,.,. HLAC. N. 2 HLAC Fig. 2
CHLAC 1 2 3 3,. (CHLAC), 1).,.,, CHLAC,.,. Suspicious Behavior Detection based on CHLAC Method Hideaki Imanishi, 1 Toyohiro Hayashi, 2 Shuichi Enokida 3 and Toshiaki Ejima 3 We have proposed a method for
Influence of Material and Thickness of the Specimen to Stress Separation of an Infrared Stress Image Kenji MACHIDA The thickness dependency of the temperature image obtained by an infrared thermography
ディスプレイと携帯端末間の通信を実現する映像媒介通信技術
Data Transfer Technology to Enable Communication between Displays and Smart Devices 倉木健介 中潟昌平 田中竜太 阿南泰三 あらまし Abstract Recently, the chance to see videos in various places has increased due to the speedup
1
5-3 Photonic Antennas and its Application to Radio-over-Fiber Wireless Communication Systems LI Keren, MATSUI Toshiaki, and IZUTSU Masayuki In this paper, we presented our recent works on development of
22.Q06.Q
57 2220045770 Journal of the University of the Air, No. 222004pp.5770 1 2 Participatory Measures of Protection for Crested Ibis Nipponia Nippon and its Habitat Comparative Case Study of Yang County, China
GUNSLINGER GIRL
009 GUNSLINGER GIRL. TV 1 343 2009 009 GUNSLINGER GIRL 60 70 2 344 SF anime 3 345 2009 009 1968 TV 68 TV 12 1980 009 009 SF 009 4 5 GUNSLINGER GIRL GUNSLINGER GIRL 2003 6 346 . TV 009 7 009 8 347 2009
Vol. 48 No. 3 Mar PM PM PMBOK PM PM PM PM PM A Proposal and Its Demonstration of Developing System for Project Managers through University-Indus
Vol. 48 No. 3 Mar. 2007 PM PM PMBOK PM PM PM PM PM A Proposal and Its Demonstration of Developing System for Project Managers through University-Industry Collaboration Yoshiaki Matsuzawa and Hajime Ohiwa
EP-3 事件と中国の危機管理 : 2001年米中軍用機接触事故の今日的教訓
Kwansei Gakuin University Rep Title Author(s) EP-3 事件と中国の危機管理 : 2001 年米中軍用機接触事故の今日訓 Inoue, Ichiro, 井上, 一郎 Citation 総合政策研究, 43: 1-13 Issue Date 2013-06-10 URL http://hdl.handle.net/10236/10942 Right http://kgur.kawansei.ac.jp/dspace
Key Words: probabilisic scenario earthquake, active fault data, Great Hanshin earthquake, low frequency-high impact earthquake motion, seismic hazard map 3) Cornell, C. A.: Engineering Seismic
４章 困難な課題への挑戦 核酸結晶学
4-2-6. I. a. DNA DNA RNA Watson & Crick 1953 b. DNA DNA DNA DNA RNA Cech et al. 1981 RNA in vitro selection DNA Breaker & Joyce 1994 2% 50% 30% International Human Genome Sequencing Consortium 2001 1 DNA
千葉県における温泉地の地域的展開
1) 1999 11 50 1948 23) 2 2519 9 3) 2006 4) 151 47 37 1.2 l 40 3.6 15 240 21 9.2 l 7. 210 1972 5) 1.9 l 5 1 0.2 l 6 1 1972 1.9 0.4 210 40-17- 292006 34 6 l/min.42 6) 2006 1 1 2006 42 60% 5060 4050 3040
258 5) GPS 1 GPS 6) GPS DP 7) 8) 10) GPS GPS 2 3 4 5 2. 2.1 3 1) GPS Global Positioning System
Vol. 52 No. 1 257 268 (Jan. 2011) 1 2, 1 1 measurement. In this paper, a dynamic road map making system is proposed. The proposition system uses probe-cars which has an in-vehicle camera and a GPS receiver.
1. Precise Determination of BaAl2O4 Cell and Certification of the Formation of Iron Bearing Solid Solution. By Hiroshi UCHIKAWA and Koichi TSUKIYAMA (
1. Precise Determination of BaAl2O4 Cell and Certification of the Formation of Iron Bearing Solid Solution. By Hiroshi UCHIKAWA and Koichi TSUKIYAMA (Central Research Laboratory, Onoda Cement Co., Ltd.,
<332D985F95B62D8FAC93638BA795DB90E690B62E706466>
No.50 pp.5568, 2014 Komazawa Journal of Geography Geography of Religion in Postwar Japan (2) ODA Masayasu 1 10 200211 1219972006 Keywords: geography of religion, Bibliography of Geography, Islam, sacred
<95DB8C9288E397C389C88A E696E6462>
2011 Vol.60 No.2 p.138 147 Performance of the Japanese long-term care benefit: An International comparison based on OECD health data Mie MORIKAWA[1] Takako TSUTSUI[2] [1]National Institute of Public Health,
42 1 Fig. 2. Li 2 B 4 O 7 crystals with 3inches and 4inches in diameter. Fig. 4. Transmission curve of Li 2 B 4 O 7 crystal. Fig. 5. Refractive index
MEMOIRS OF SHONAN INSTITUTE OF TECHNOLOGY Vol. 42, No. 1, 2008 Li 2 B 4 O 7 (LBO) *, ** * ** ** Optical Scatterer and Crystal Growth Technology of LBO Single Crystal For Development with Optical Application
Introduction ur company has just started service to cut out sugar chains from protein and supply them to users by utilizing the handling technology of
Standard PA-Sugar Chain Catalogue Masuda Chemical Industries Co., LTD. http://www.mc-ind.co.jp Introduction ur company has just started service to cut out sugar chains from protein and supply them to users
Table 1. Assumed performance of a water electrol ysis plant. Fig. 1. Structure of a proposed power generation system utilizing waste heat from factori
Proposal and Characteristics Evaluation of a Power Generation System Utilizing Waste Heat from Factories for Load Leveling Pyong Sik Pak, Member, Takashi Arima, Non-member (Osaka University) In this paper,
研究成果報告書
20 6 UAA UGA UAG 20 Pyl. Pyl (AlocLys) N(epsilon)-benzyloxycarbonyl-lysine (ZLys) N(epsilon)-(o-azidobenzyloxycarbonyl) -lysine oazzlys (Yanagisawa et al., 2008b) N(epsilon)-(p-trifluoromethyldiazirinylbenzyloxy
041-057_’¼Œì
542012 4157 Nishino Toshiaki The purpose of this paper is to analyze the present conditions of the mountain villages of Japan in the early 21 st century. The revolution of fuel sources from a predominance
社会技術論文集
STS Studies on Kanemi-Yusho Case with Special Interests on the Accident Investigation 1 1 M.Sc. (E-mail:tnakaji@plum.ocn.ne.jp) 1968 PCB 1. 2003 15 5 23 9;37 1900 2003 33 2 2 BSE 3 8; 14 2001 9 BSE 1 BSE
2 146
28 2004 pp. 145 159 1 Received October 29, 2004 In 1999, North Korea reversed the negative economic growth of the 90s, and displayed a positive trend which, although weak, was maintained at 1.8% in 2003.
研究成果報告書
COE 8 4 月 13 3 17-19,20-22 -COO - NH4 + 7 Fig.1 た 2 プ Fig.1 =0.154mol/L =0.1ml/min = 0.665g=20 PET AA PET Fig.2 AA AA PET PET AA Fig.2 PET AA-graft PET (3.17%, 6.37%) Fig.3 Fig.4 14 RDSv/h=1.10*e (-0.055d)
RT-PCR プロトコール.PDF
Real -Time RT-PCR icycler iq Bio Rad RT-PCR RT-PCR 1 icycler iq Bio Rad icycler iq 30 2 Ready-To-Go T-Primed First-Strand Kit (amersham pharmacia biotech) Ready-To-Go T-Primed First-Strand Kit QuantiTect
Database Center for Life Science Online Service
36 蛋 白質 核酸 酵素 Vol.42 No.13(1997) くに 蛋 白質 問 の 認 識 ドメ イ ン と し て の 役 割 に 重 点 を お い て 述 べ て み た い ま た,筆 たLIMド 者 らに よって見 いだ され メ イ ン を もっ プ ロ テ イ ンキ ナ ー ゼ,LIMキ ー ゼ(LIMK)フ ナ ァ ミ リー に つ い て 最 近 の 知 見 を紹 介 す る
16_.....E...._.I.v2006
55 1 18 Bull. Nara Univ. Educ., Vol. 55, No.1 (Cult. & Soc.), 2006 165 2002 * 18 Collaboration Between a School Athletic Club and a Community Sports Club A Case Study of SOLESTRELLA NARA 2002 Rie TAKAMURA
Author Workshop 20111124 Henry Cavendish 1731-1810 Biot-Savart 26 (1) (2) (3) (4) (5) (6) Priority Proceeding Impact factor Full paper impact factor Peter Drucker 1890-1971 1903-1989 Title) Abstract
PDCA
PDCA / / -- -- -- -- -- -- % % --- --- - No.--- --- --- A B C D + + + + + + + + + A B C D........................ --- OJT PDCA Eliminate Combine ECRS Rearrange Simplify -- - BKC IT BKC BKC APU -- :
地質調査研究報告/Bulletin of the Geological Survey of Japan
Takayuki Uchino and Makoto Kawamura (2010) Glaucophane found from meta-basalt in the Nedamo Terrane, Northeast Japan, and its geologic significance. Bull. Geol. Surv. Japan, vol. 61 (11/12), p. 445-452,
,,.,.,,.,.,.,.,,.,..,,,, i
22 A person recognition using color information 1110372 2011 2 13 ,,.,.,,.,.,.,.,,.,..,,,, i Abstract A person recognition using color information Tatsumo HOJI Recently, for the purpose of collection of
37-4.indd
ISSN 0916-3328 東京大学アイソトープ総合センター VOL. 37 NO. 4 2007. 3. 26 アイソトープ総合センターの将来展望 40 18 18 20 3 800 1/3 1300 14 18 20 3 19 2 生きた細胞内の分子過程を 時間 空間計測する蛍光プローブの開発 1. はじめに Fura-2 Fura-2 198 1, 2 3, 4 2. 生体脂質分子の機能とその分析
Kyushu Communication Studies 第２号
Kyushu Communication Studies. 2004. 2:1-11 2004 How College Students Use and Perceive Pictographs in Cell Phone E-mail Messages IGARASHI Noriko (Niigata University of Health and Welfare) ITOI Emi (Bunkyo
PowerPoint プレゼンテーション
バイオインフォマティクスにおける ゲノム情報の基礎知識 Database of Pathogenic Variants もくじ 1. ゲノム 1-1 DNAの構造 1-2 DNAの複製 1-3 RNA 1-4 セントラルドグマ 1-5 構造遺伝子 1-6 コドン 3. 変異 3-1 遺伝子の変異 3-2 病的変異の種類 2. 転写と翻訳 2-1 転写 (DNA mrna) 2-2 転写に関わる領域
WATOC2005 Best Poster Diamond Certificate α -(BEDT-TTF) 2 IBr 2 H.turkmenica X H C H O Brønsted Al K Electronic structure of CeNiGe 2 x Si x studied by Ce 4d-4f resonant and angleresolved photoemission
Financing through Stadium Securitization \ Case Study of Professional Football Clubs in England \ õ Yoshiaki IIDA * Abstract The purpose of this study was to clarify the way in which the stadium securitization
weak ferromagnetism observed on Shimotokuyama and Ayumikotan natural crystals behaves as pre dicted by Dzyaloshinsky and Moriya, while Wagasennin and
Magnetic Behavior of a-fe2o3, I. Origin of Weak Ferromagnetism and Magnetic Characteristics Masako IWATA (The Research Institute for Iron, Steel and Other Metals, Tohoku University, Katahiracho, Sendai)
タンパク質の合成と 構造 機能 7 章 +24 頁 転写と翻訳リボソーム遺伝子の調節タンパク質の構造弱い結合とタンパク質の機能
タンパク質の合成と 構造 機能 7 章 +24 頁 転写と翻訳リボソーム遺伝子の調節タンパク質の構造弱い結合とタンパク質の機能 タンパク質の合成 セントラル ドグマによると 遺伝子が持つ情報は タンパク質を合成することで発現 (Expression) される それは 2 段階の反応で進行する DNA 転写 (Transcription) DNA の塩基配列から mrna の塩基配列へ染色体の
<4D F736F F D20D2E5E7E8F1FB E3EEE45FE8F1EFF0>
13 1 71 71 71 71 7 1 7 1 7 1 7 1 71 71 71 71 71 71 7 (1 71 71 71 7 1 71 7) 1 7 1 71 71 71 71 71 71 7 1 71 7 1 7 1 71 71 71 71 7 1 71 71 71 71 71 71 71 7 1 71 71 71 71 7, 1 71 71 71 71 71 71 7 1 7 1 71
untitled
(a) (b) (c) (d) (e) (f) (g) (f) (a), (b) 1 He Gleiter 1) 5-25 nm 1/2 Hall-Petch 10 nm Hall-Petch 2) 3) 4) 2 mm 5000% 5) 1(e) 20 µm Pd, Zr 1(f) Fe 6) 10 nm 2 8) Al-- 1,500 MPa 9) 2 Fe 73.5 Si 13.5 B 9 Nb
2 194
32 2008 pp. 193 210 1 Received October 31, 2008 The Japanese auxiliary verbs in benefactive construction can be classified in terms of the following two semantic functions: ones that only represent the
1..FEM FEM 3. 4.
008 stress behavior at the joint of stringer to cross beam of the steel railway bridge 1115117 1..FEM FEM 3. 4. ABSTRACT 1. BackgroundPurpose The occurrence of fatigue crack is reported in the joint of
, (GPS: Global Positioning Systemg),.,, (LBS: Local Based Services).. GPS,.,. RFID LAN,.,.,.,,,.,..,.,.,,, i
25 Estimation scheme of indoor positioning using difference of times which chirp signals arrive 114348 214 3 6 , (GPS: Global Positioning Systemg),.,, (LBS: Local Based Services).. GPS,.,. RFID LAN,.,.,.,,,.,..,.,.,,,
yasi10.dvi
2002 50 2 259 278 c 2002 1 2 2002 2 14 2002 6 17 73 PML 1. 1997 1998 Swiss Re 2001 Canabarro et al. 1998 2001 1 : 651 0073 1 5 1 IHD 3 2 110 0015 3 3 3 260 50 2 2002, 2. 1 1 2 10 1 1. 261 1. 3. 3.1 2 1
IHIMU Energy-Saving Principle of the IHIMU Semicircular Duct and Its Application to the Flow Field Around Full Scale Ships IHI GHG IHIMU CFD PIV IHI M
IHIMU Energy-Saving Principle of the IHIMU Semicircular Duct and Its Application to the Flow Field Around Full Scale Ships IHI GHG IHIMU PIV IHI Marine United Inc. ( IHIMU ) has already developed several
ÿþ
Abstract In this paper, we analyze the mechanism of open-source development, in which anybody can join and leave to the collaboration to develop complex software system, as implausible phenomena. Here,
KII, Masanobu Vol.7 No Spring
KII, Masanobu 1 2 3 4 5 2 2.1 1945 60196075 19759019904 002 Vol.7 No.1 2004 Spring 1194560 1 1946 2 195328 3 4 1958 1960 2196075 5 6 7 8 1946 2 1972 1/23/4 1974 1968481973 1969 3197590 9 1980 1987 41990
Table 1. Reluctance equalization design. Fig. 2. Voltage vector of LSynRM. Fig. 4. Analytical model. Table 2. Specifications of analytical models. Fig
Mover Design and Performance Analysis of Linear Synchronous Reluctance Motor with Multi-flux Barrier Masayuki Sanada, Member, Mitsutoshi Asano, Student Member, Shigeo Morimoto, Member, Yoji Takeda, Member
Core Ethics Vol.
Core Ethics Vol. Core Ethics Vol. . % % % Core Ethics Vol. %, Core Ethics Vol. % % % -. %. Core Ethics Vol. a b : Core Ethics Vol. pp... pp.. pp... pp. pp.. pp...pp.... pp. pp. pp.. pp.. Vol.. pp... pp..
00.\...ec5
Yamagata Journal of Health Science, Vol. 6, 23 Kyoko SUGAWARA, Junko GOTO, Mutuko WATARAI Asako HIRATUKA, Reiko ICHIKAWA Recently in Japan, there has been a gradual decrease in the practice of community
1 Credit: NASA/JPL-Caltech/R. Hurt SSC-Caltech ,
397 0101 10762 30 e-mail: matsunaga@ioa.s.u-tokyo.ac.jp 8 3 20 2 3 3 7 2 3 1. 100 1912 1 1 4 12 10 3 1 20 2,500 2 1 3 4 374 2012 6 1 Credit: NASA/JPL-Caltech/R. Hurt SSC-Caltech 3 2. 5 3 6 100 7 8, 9 10
SPring8菅野印刷.PDF
20021219Spring-8 Ah/kg Ah/dm 3 Li -3.01 540 3860 2090 Na -2.71 970 1160 1140 Al -1.66 2690 2980 8100 Zn -0.76 7140 820 5800 Fe -0.44 7850 960 7500 Cd -0.40 8650 480 4100 Pb -0.13 11340 260 2900 H 2 0
Fig. 2 Signal plane divided into cell of DWT Fig. 1 Schematic diagram for the monitoring system
Study of Health Monitoring of Vehicle Structure by Using Feature Extraction based on Discrete Wavelet Transform Akihisa TABATA *4, Yoshio AOKI, Kazutaka ANDO and Masataka KATO Department of Precision Machinery
磁気測定によるオーステンパ ダクタイル鋳鉄の残留オーステナイト定量
33 Non-destructive Measurement of Retained Austenite Content in Austempered Ductile Iron Yoshio Kato, Sen-ichi Yamada, Takayuki Kato, Takeshi Uno Austempered Ductile Iron (ADI) 100kg/mm 2 10 ADI 10 X ADI
(2) (2) (3) (3) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux LineGene (9) 1 RT-PCR (10) (12) (13) F. Hoffmann-La Roche Ltd. Roche Molecular
05-8 SYBR Green Realtime PCR Master Mix (Code No. QPK-201, QPK-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A3314K (2) (2) (3) (3) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux
1 [1, 2, 3, 4, 5, 8, 9, 10, 12, 15] The Boston Public Schools system, BPS (Deferred Acceptance system, DA) (Top Trading Cycles system, TTC) cf. [13] [
![1 [1, 2, 3, 4, 5, 8, 9, 10, 12, 15] The Boston Public Schools system, BPS (Deferred Acceptance system, DA) (Top Trading Cycles system, TTC) cf. [13] [](/thumbs/91/106628466.jpg "1 [1, 2, 3, 4, 5, 8, 9, 10, 12, 15] The Boston Public Schools system, BPS (Deferred Acceptance system, DA) (Top Trading Cycles system, TTC) cf. [13] [")
Vol.2, No.x, April 2015, pp.xx-xx ISSN xxxx-xxxx 2015 4 30 2015 5 25 253-8550 1100 Tel 0467-53-2111( ) Fax 0467-54-3734 http://www.bunkyo.ac.jp/faculty/business/ 1 [1, 2, 3, 4, 5, 8, 9, 10, 12, 15] The