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5 1) Asa, S. L., et al.: Immunohistochemical localization of neuron-specific enolase in the human hypophysis and pituitary adenoma, Arch. Pathol. Lab. Med., 108: 40 `43, ) Chi, B. H. and Kim, R. C.: Immature oligodendroglial cells in developing human fetal spinal cord contain immunoreactive glial fibrillary acidic protein (GFAP), J. Neuro. Pathol. Exp. Neurol., 42: 325, ) Eng, L. F., et al.: An acidic protein isolated from brous astrocytes, Brain Res., 28: 351 `354, fi Summary Immunocytochemical studies of 45 cases of brain tumors (astrocytoma Egrade 2 7, pleomorphic xanthoastrocytoma 1, astroblastoma 1, anaplastic astrocytoma 8, glioblastoma 9, oligodendroglioma 4, ependymoma 1, meningioma 6, neurinoma 2, pituitary adenoma 3, metastatic carcinoma 3) were done using six antibodies of intermediate filaments and neuron specific proteins. GFAP was very useful to discriminate glial tumors and ependymoma from other tumors because glial tumors and ependymoma only were positive, and gliomas showed decrease in high-malignancy. Positive NFP seen in cases of glioblastomas and pituitary adenomas was considered to be due to dysdifferentiation. Positive Vimentin seen in gliomas, which reversely correlated with positivity of GFAP, was explained by dedifferentiation. Cytokeratin was very useful to discriminate metastatic carcinoma and pituitary adenomas from other primary brain tumors, because metastatic carcinomas and pituitary adenomas only were positive. NSE showed no specifically for a certain type of brain tumors. S-100 was very useful to discriminate neurinoma from meningioma, because the both cytologically resemble each another and neurinomas were intense positive and meningiomas were negative or feeble positive. 6) Franke, W. W., et al.: Different intermediated-sized laments distinguished by immunofluorescence fi microscopy, Proc. Nat. Acad. Sci. USA, 75: 5034 `5038, ) Inoue, M., et al.: Immunohistochemical demonstration of neuron-specific enolase in gynecologic malignant tumors, Cancer, 55: 1686 `1690, ) Lauriola, L., et al.: Immunohistochemical detection of folliculo-stellate cells in human pituitary adenomas, Virchows. Arch (cell Pathol), 47: 189 `197, ) McMenemey, W. H.,: An appraisal of smear, diagnosis in neurosurgery, Am. J. Clin. Path., 33: , ) Moore, B. W. and McGregor, D.: Chromatographic and electrophoretic fractionation of soluble proteins of brain and liver, J. Biol. Chem., 240: ,

6 ) Nakajima, T., et al.: Enolase distribution in human brain tumors, retinoblastomas and pituitary immunocytochemical study, Brain Res., 200: 13 `21, adenomas, Brain Res., 38: 215 `222, ) Shelanski, M.L., et al.: Isolation of filament from brain, Science., 174: , ) Roessmann, V., et al.: Glialfibrillary acidic protein (GFAP) in ependymal cells during development; An 22) Vinores, S. A., et al.: Immunohistochemical demonstration of the CNS and other tissues, Arch. Pathol. Lab. Med., 108: 536 `540, 1984.

7 812 写 真1 日本臨床細胞学会雑誌 Astrocytoma(G2).腫 を 示 す. 写 真2 Glioblastoma.一 瘍 細 胞 の細 胞 突 起 が 陽 性 GFAP, 部 の 腫 瘍 細 胞 質 内 に 陽 性 を 示 す. (NFP, 写 真3 Glioblastoma.ほ 示 す, Metastatic す. 写 真5 Ependymoma.ロ と ん どの 腫 瘍 細 胞 質 内 に陽 性 を ( Vimentin, 写 真4 carcinoma,腫 瘍 細 胞 質 内 に陽 性 を 示 (Cyto keratin, ゼ ッ ト形 成 部 に 陽 性 を 示 す. (NSE, 写 真6 Neurinoma.腫 400) 瘍 細 胞 質 内 に 陽 性 を 示 す. (S-100,

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