1 HLA MHC 2017; 24 (2) HLA HLA 1967 HLA HLA HLA HLA 1980 HLA HLA ABO HLA 2000 HLA DSA 2010 HLA HLA HLA HLA HLA HLA HLA HLA HLA GVHD HLA HLA
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1 Major Histocompatibility Complex 2017; 24 (2): 抄録集 1 HLA
2 1 HLA MHC 2017; 24 (2) HLA HLA 1967 HLA HLA HLA HLA 1980 HLA HLA ABO HLA 2000 HLA DSA 2010 HLA HLA HLA HLA HLA HLA HLA HLA HLA GVHD HLA HLA HLA HLA 151
3 MHC 2017; 24 (2) 1 HLA QCWS 2) QCWS 2) MHC HLA QC QC HLA Quality Control Workshop QCWS DNA DNA QCWS 29 QCWS HLA HLA HLA T antibody mediated rejection: ABMR 1980 ABMR donor specific antibody: DSA de novo DSA ABMR 152
4 1 HLA MHC 2017; 24 (2) HLA HLA HLA GVHD HLA / HLA HLA HLA DSA HLA HLA1 HLA HLA PT/Cy HLA HLA-A -B -DRB1 6 2 HLA 1 HLA DSA SAB SAB LABSreen- Mixed Mixed HLA Scr LABSreen- PRA PRA Scr 67 MDSRAEB II HLA 3 17 Scr 153
5 MHC 2017; 24 (2) Scr Mixed Class I Ratio Class II Ratio Cut Off Ratio3.0 SAB CutOff nmfi1000 DQ nmfi Scr PRA Class I Class II SAB Class I A2 nmfi Class II DQ nmfi Class I SAB 1 HLA A2 nmfi Mixed Cut Off Ratio1.5 Ratio3.0 Cut Off SAB Cut Off Scr Cut Off HLA HLA HLA DSA DSA HLA DSA DSA rituximab 4 Single Antigen DSA nmfi 7814 rituximab DSA DSA nmfi DSA 154
6 1 HLA MHC 2017; 24 (2) HIGH-RESOLUTION HLA TYPING BY NXTYPE TM NGS HLA TYPING KIT Seik-Soon Khor, Yuki Hitomi, Yuko Okudaira 2), Anri Masuya 2), Yuki Ozaki 3), Mayumi Ueta 4), Ken Nakatani, Masaki Nagato 5), Takahiro Ogawa 5), Chie Sotozono 4), Shigeru Kinoshita 4), Hidetoshi Inoko 2), Katsushi Tokunaga Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan 2) GenoDive Pharma Inc., Honatsugi, Kanagawa, Japan 3) The Center of Medical Innovation and Translational Research, Graduate School of Medicine, Osaka University, Osaka, Japan 4) Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan 5) Research and Development Section, Wakunaga Pharmaceutical Co., Takata, Hiroshima, Japan The human MHC region has been shown to be associated with a wide range of diseases. The recent advancement in the next generation sequencing (NGS) technology has definitely helped in increasing the resolution (up to 4-field) of HLA genotyping. However, it remains a challenge in interpreting the results from NGS platform due to biases includin-systematic sequencing error by sequencing platform, difference in the sensitivity and specificity of HLA calling algorithm, ability to resolve HLA alleles ambiguity by incorporating phasing information, and more importantly incompleteness of the current IMGT database. In order to access the sensitivity and specificity of the NXType TM NGS HLA typing kit (One Lambda), a total of 105 Japanese samples were sequenced for HLA class I genes using the Ion Torrent Personal Genome Machine (PGM) following the NXType protocol. HLA calling was performed using the HLATypeStream v with IMGT/HLA databses of Concordance rates were evaluated by comparing HLATypeStream results with Luminex-based HLA typing result for up to 2-field result. With the default setting of HLATypeStream and without manual interpretation of the results, the allelic concordance is more than 98% for HLA-A, HLA-B and HLA-C. Careful inspection of the final results and occasionally manual interpretations of results are necessary especially for rare or novel HLA alleles. Long-range Short-range PCR NGS HLA PCR 3 PCR HLA short-range long-range short-range Long-range 3 HLA PCR bp Long-range 155
7 MHC 2017; 24 (2) 1 HLA NGS Ion Torrent system ThermoFisher Scientific MiSeq illumina NGS HLA DNA 1 DNA phase ambiguity index barcode NGS NGS PCR 2 3 NGS PCR NGS NGS HLA KIR NK HLA KIR 150 kb
8 1 HLA MHC 2017; 24 (2) KIR NK HLA KIR KIR 157
16 MHC 2018; 25 (1) 参加費 1 2, , ,000 会議等 :00 13: :00 13: :20 会場地図 TEL JR 350 m 57
Major Histocompatibility Complex 2018; 25 (1): 56 82 抄録集 16 2018 3 3 7 2 4 43 TEL 06 6962 7001 594 1101 840 TEL 0725 56 1220 56 16 MHC 2018; 25 (1) 参加費 1 2,000 2 1,000 3 3,000 会議等 1 3 3 13:00 13:10 2 3
MHC 2013; 20 ( 2 QC QC DNA-QC 3. QCWS 集会参加および参加証明書発行 QCWS QCWS QCWS 解析方法 DNA-QC Luminex SSO SSO SSP SBT QC FlowPRA Lab Screen WAK Flow ICFA 4 HL
Major Histocompatibility Complex 2013; 20 (: 13 16 QCWS # 1. ワークショップの経過 24 1 QCWS HP QCWS 24 2 57 DNA-QC 53 QC 38 1 20 QCWS DNA-QC QC 4 QCWS 4 4 4 16 QCWS 5 21 57 DNA-QC 53 QC 37 5 6 7 HP 8 25 HP 2. QCWS
Major Histocompatibility Complex 2018; 25 (2): 抄録集 2 HLA A 3 128
Major Histocompatibility Complex 2018; 25 (: 128 138 抄録集 2018 4 14 A 3 128 MHC 2018; 25 ( HLA 2013 10.5 10 8 12 10 8 2016 CD34 + HLA NC 10 8 CD34 + CD34 + 10 6 NC10.5 2013.Apr 2014.Mar NC11 2014.Apr 2016.Jun
22 S2-4 教育講演 ( 認定制度講習会 ) 9 月 14( 土 ) 10:00 ~ 12:00 座長 EL-1 EL-2 EL-3 HLA 1,2) 1) 2) HLA HLA 17th QC ワークショップ集会 9 月 14 日 ( 土 ) 13:00 ~ 16:00 タイピング結果解析 1
22 特別講演 Ⅰ 9 月 15 日 ( 日 ) 13:40 ~ 14:40 座長 SL-1 ips 特別講演 Ⅱ( スポンサード ランチ付き ) 9 月 16 日 ( 月 ) 12:00 ~ 13:00 座長 SL-2 HLA シンポジウム Ⅰ 9 月 15 日 ( 日 ) 14:40 ~ 16:40 免疫寛容を誘導する養子免疫療法 : その基礎と臨床応用 座長 S1-1 CREST JST S1-2
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Major Histocompatibility Complex 2016; 23 (3): 168 184 1) 2) 3) 4) 5) 1) 6) 7) 8) 1) 2) 3) 4) HLA 5) 6) 7) 8) HLA 11 25 28 10 22 1 4 61 44 6 1 10 11 5 HLA 60 3 27 3 5 9 5 10 19 5 50 22.4 5.9 8.2% 78.7%
MHC 2013; 20 (1) 11 参加費 1 2, , ,000 会議等 :50 14: :30 13: :00 会場地図 参天製薬株式会社本社案内図 TEL 新大阪駅より
Major Histocompatibility Complex 2013; 20 (1): 63 89 抄録集 11 2013 2 2 3 9 19 TEL: 06 6321 7000 630 0293 1248 1 TEL: 0743 77 0880 E-mail: tubaki@nara.med.kindai.ac.jp 63 MHC 2013; 20 (1) 11 参加費 1 2,000 2
日本組織適合性学会誌第23巻2号
Major Histocompatibility Complex 2016; 23 (2): 115 122 HLA 1 1) 1) HLA MHC 1900 HLA HLA キーワード : HLA HLA HLA HLA 2 HLA HLA HLA 3 1 1 HLA HLA 図 1 2016 6 30 2016 8 2 105 0013 1 10 14 3 5 TEL: 03 5776 0048
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Major Histocompatibility Complex 2016; 23 (1): 45 71 抄録集 14 2016 2 6 7 2 4 43 TEL 06 6962 7001 HLA 600 8813 134 1 2F TEL 075 313 5201 FAX 075 313 5202 E-mail:hla@hla.or.jp 45 MHC 2016; 23 (1) 第 14 回日本組織適合性学会近畿地方会
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Major Histocompatibility Complex 2015; 22 (1): 44 66 抄録集 13 2015 2 7 7 2 4 43 TEL: 06 6962 7001 589 8511 377 2 TEL: 072 366 0221 E-mail: yuketsu@med.kindai.ac.jp 44 MHC 2015; 22 (1) 第 13 回日本組織適合性学会近畿地方会
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