AriaMx PCR AriaMx PCR PCR AriaMx PCR 0.2 ml 96 6 PCR Ct SYBR Green I PCR Tm TaqMan PCR DNA HRM TaqMan AriaMx 8 Multiple Experiment Analysis PDF RDML P

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1 AriaMx PCR See Deeper. Reach Further.

2 AriaMx PCR AriaMx PCR PCR AriaMx PCR 0.2 ml 96 6 PCR Ct SYBR Green I PCR Tm TaqMan PCR DNA HRM TaqMan AriaMx 8 Multiple Experiment Analysis PDF RDML PCR ROX Rn MIQE AriaMx PC PC 8 PC PC 2 AriaMx Real-Time PCR Solution

3 PCR ml 96 Brilliant III Ultra-FAST QPCR master mix SYBR Green I 43 qpcr Brilliant III Ultra-Fast QPCR Master Mix Cycle 40 Cycles 1 Cycle PC LAN 1 PC USB AriaMx Real-Time PCR Solution 3

4 AriaMx LED QPCR FAM / SYBR nm nm HEX / VIC nm nm Cy nm nm ROX / TexasRed nm nm Cy nm nm 2 AriaMx QPCR FAM VIC Tx- Red Cy5 4 AriaMx Real-Time PCR Solution

TaqMan Allele discrimination 2 TaqMan Dual

5 HRM Class IV SNP A and T Allele AriaMx Brilliant HRM Ultra-FAST Loci Master Mix bp Class IV SNP Tm 0.13 SNP Normalized Melt Normalized Derivative Melt -dr Difference Plot -R Comparative quantification FAM VIC TaqMan MIQE Pfaffl TaqMan Allele discrimination 2 TaqMan Dual color plot Dual color plot Cq Multiple Experiment Analysis AriaMx Real-Time PCR Solution 5

6 PDF RDML RDML Realtime PCR Data Markup Language MIQE RDML 6 AriaMx Real-Time PCR Solution

7 Reference dye normalization R AriaMx Mx3000P ROX ROX Rn A AriaMx B A AriaMx B AriaMx Real-Time PCR Solution 7

AriaMx PCR AriaMx PCR 1 1 AriaMx PCR 1 AriaMx PCR 192-8510 9-1 0120-477-111mail

8 AriaMx PCR AriaMx PCR 1 1 AriaMx PCR 1 AriaMx PCR mail _japan@agilent.com AriaMx PC Windows 7 Professional and Ultimate editions 32 bits 64 bits Microsoft Office Professional Edition GHz Dual Core 2 GB 40 GB 1024 x x 1024 * LAN 1 SPEED SUCCESS OPEN REAGENTS 8 AriaMx Real-Time PCR Solution

9 AriaMx PCR LED x8 / - x8 / FAM/SYBR HEX/VIC ROX/TexasRed Cy3 Cy5 6 3 / 96 Well µl AriaMx V AC, 50 / 60 Hz 11 A % 7, kg 50 cm x 46 cmx 42 cm 0.2 ml 8 96 Windows 7 CD 1 AriaMx PCR FAM 6 / 3 / / Ct < SYBR % AriaMx Real-Time PCR Solution 9

DNA, RNA, TapeStation ScreenTape RNA RNA

RNA Sample intensity FU 5,000 4,000 3,000 2,000

10 DNA / RNA Agilent 2100 / Agilent 2200 TapeStation Agilent2100 Agilent2200 TapeStation DNA, RNA, TapeStation ScreenTape RNA RNA Integrity Number RIN total RNA TapeStation RIN 8.9 RIN 4.5 RIN 6.5 RIN 2.3 RIN HPRT1 5' 3' RNA Integrity Number RIN total RNA PCR RNA Sample intensity FU 5,000 4,000 3,000 2,000 1,000 DNA TapeStation DNA Size bp Size bp 1,000 1,500 TapeStation 10 AriaMx Real-Time PCR Solution

11 AriaMx PCR PCR SYBR Green Assay Brilliant III Ultra-Fast SYBR Green QPCR Master Mix Brilliant III Ultra-Fast SYBR Green QPCR Master Mix, Plus ROX Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix, 1-step 400 Probe Assay Brilliant III Ultra-Fast QPCR Master Mix Brilliant III Ultra-Fast QPCR Master Mix, Plus ROX Brilliant III Ultra-Fast QRT-PCR Master Mix, 1-step 400 HRM Assay Brilliant HRM Ultra-Fast Loci Master Mix 200 cdna AffinityScript QPCR cdna synthesis Kit 50 RNA QPCR Reference Total RNA, Human 25 µg QPCR Reference Total RNA, Mouse 25 µg QPCR StrataClone PCR Cloning Kit StrataPrep DNA Gel Extraction Kit StrataPrep PCR Purification Kit StrataPrep Plasmid Miniprep Kit 50 QPCR Alien QRT-PCR Inhibitor Alert Alien Reference RNA QRT-PCR Detection Kit FAM detection Alien Reference RNA QRT-PCR Detection Kit VIC detection 100 QPCR SideStep II PRT-PCR Master Mix, 1-Step SideStep Lysis and Stabilization Buffer 10 ml SideStep II Cell Lysate Analysis Kit 100 NGS G4880A QPCR NGS Library Quant Kit, Illumina GA 1 G4881A QPCR NGS Library Quant Kit, AB SOLiD HRM AriaMx Calibration Kit SYBR reference plate 1 AriaMx PCR AriaMx 96 well plates, Skirted and Low Profile AriaMx 96 well plates, Skirted and Rigid AriaMx 96 well optical plates, Non-skirted Low Profile AriaMx Adhesive Plate Seals AriaMx Tube Strips Mx3000p Optical Strip Caps 120 AriaMx Real-Time PCR Solution 11

12 * This is a Licensed Real-Time Thermal Cycler(s) or Licensed Real-Time Temperature Cycling I nstrument(s) under ABI s United States Patent No. 6,814,934 and corresponding claims in non-u.s. counterparts thereof, for use in research and for all other applied fields except human in vitro diagnostics. No right is conveyed expressly, by implication or by estoppel under any other patent claim. Agilent is a registered trademark of Agilent Technologies. Excel and PowerPoint are registered trademarks of the Microsoft Corporation. SYBR is a registered trademark of Molecular Probes. Scorpions is a trademark of DxS Ltd. TaqMan is a registered trademark of Roche Molecular Systems, Inc. TRI Reagent is a registered trademark of Molecular Research Center, Inc. FAM, TET, HEX, VIC, JOE, TAMRA, and ROX are trademarks of Applera Corporation or it s subsidiaries in the US and certain other countries. Cy 3 and Cy 5 are trademarks of Amersham Biosciences. RiboGreen is a registered trademark of Molecular Probes. / mail _japan@agilent.com AgilentGenomics.jp Agilent Technologies, Inc Printed in Japan, Nov. 14, JAJP

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