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1 Key words: disinfectant, amphoteric surfactant, ethanol, Mycobacterium tuberculosis

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3 Table 1 Bactericidal effects of cresol soap and an amphoteric surfactant (ADG) against M. tuberculosis H37Rv : The numbers of the survived cells were 500 cfu/0.1 ml. Table 2 Bactericidal effects of an amphoteric surfactant (ADG), ADG containing ethanol (Et-ADG) and ethanol against M. tuberculosis H37Rv The numbers of the survived cells were 500 cfu/0.1 ml. Table 3 Bactericidal effects of an amphoteric surfactant (ADG), ADG containing ethanol (Et-ADG) and ethanol against tubercle bacilli in sputum samples The numbers of the control-cells were 165 cfu/0.1 ml.

4 佐藤 94 明正 他 Fig. 1 Scanning electron microscopic findings of M. tuberculosis H37Rv treated with four defferent disinfectants. Top-left : Strong stickiness and/or fusion among cells treated with 30v/v% ethanol-1/50adg. Top-right: Some stickiness and/or fusion among cells treated with 1/50ADG. Bottom-left: A little stickiness and/or fusion among cells treated with 30v/v% ethanol. Bottom-right: No stickiness and/or fusion among untreated cells. の効 果 は,喀 痰 中 の結 核 菌 に対 して は 僅 か で あ っ ADGで た.し か し23v/v%エ ノ ー ル で 処 理 した菌(下 タ ノ ー ル を添 加 したADG溶 処 理 した 場 合 よ り若 干 少 な か った.エ 段 左 の 写 真)で タ も菌 体 表 液(23%Et-1/50ADG)で は,5分 間処 理 で 生 存 菌 面 の粘 性 化 が 観 察 さ れ た が,菌 体 の 融 合 の程 度 は 数 を ゼ ロ に した.ま た25v/v%エ タノール を添加 ADGで した 溶 液(26%Et-1/50ADG)で は3分 間 処 理 で 同 様 な効 果 が 観 察 され た. 照 の 蒸 留 水 処 理 菌(下 段 右 の 写 真)で は菌 体 の 融 合 は観 察 さ れ な か っ た. 4) 消 毒 した 結 核 菌 の走 査 電 子 顕 微 鏡 像:電 像 をFig.1に 処 理 した 場 合 よ りさ らに 少 な か っ た.対 示 す.Et-ADGで 顕 考 察 処 理 した 菌(上 段 ク レ ゾ ー ル 石 鹸 液 は そ の 臭 気 と フ ェ ノー ル類 の 左 の 写 真)で は,菌 体 表 面 の 粘 性 化 変 形 化 に伴 っ 排 水 規 制 に よ り,ほ とん ど使 用 さ れ な くな っ て き て多 数 の 菌 が 互 い に融 合 し,個 々 の菌 の形 態 が 不 て い る.そ 明 に な った も の が 多 く観 察 さ れ た.ADGで し た菌(上 段 右 の写 真)で 処理 も菌体 表 面 の粘 性 化 変 形 化 が 観 察 さ れ た が,菌 体 の 融 合 の程 度 はEt- こで,結 核 菌 に対 して比 較 的 殺 菌 効 力 を有 す る消 毒 剤 につ い て 文献 的 に調 べ た.そ の中 で 最 も有 望 な 消 毒 剤 は両 性 界 面 活 性 剤 を主 成 分 と す る消 毒 剤 で あ った4) 7). 感染 症 学 雑誌 第69巻 第1号

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6 13) Frobisher, M. & Sommermeyer, L.: A study of 8) Takeya, K., Mori, R., Tokunaga,T., Koike, M. & Hisatune, K.: Further studies of the paired Biophys. Biochem. Cytol., 9: , the effect of alcohols on tubercle bacilli & other bacteria in sputum. Am. Rev. Tuberc., 68: , 1953.

7 The Bactericidal Effects of an Amphoteric Surfactant with Ethanol against Mycobacterium tuberculosis kimasa SATO1), Hideaki OHISHI1) & Tetsuji SAKASHITA2) Bactericidal activities of an amphoteric surfactant (12 w/v% Alkyl diaminoethylglycine hydrochloride, 3 w/v% Alkyl dietylenetriaminoglycole hydrochloride, Nissan Anon #300(R), Inui Shouji Co., ADG) against Mycobacterium tuberculosis were examined independently or in cooperation with ethanol. The results obtained in the study are as follows: 1. In the treatment with the surfactant diluted with distilled water in one hundredth, the numbers of tubercle bacilli which survived were 500 cfu/0.1 ml after one minute-treatment, and 25 cfu/0.1 ml after ten minutes-treatment. On the other hand, the surfactant containing 23 v/v% ethanol decreased the bacterial numbers to seven cfu/0.1 ml after one minute and less than one cfu/0.1 ml after ten minutes. The numbers of the survived bacilli treated with the surfactant containing 20 v/v% ethanol were 500 cfu/0.1 ml after one minute-treatment, and less than one cfu/0.1 ml after ten minutes though 20 v/v% ethanol alone was ineffective in ten minutestreatment. 2. The bactericidal activities of the surfactant against tubercle bacilli in sputum samples in which 1.65 ~ 104 cfu/0.1 ml of the organism were contained were also investigated. The numbers of tubercle bacilli which survived were 25 cfu/0.1 ml after one minute-treatment with the surfactant in one fiftieth, two cfu/0.1 ml after five minutes-treatmnet and one cfu/0.1 ml after ten minutes-treatment. The numbers of the organism treated with the surfactant containing 23 v/v% ethanol were less than one cfu/0.1 ml after five minutes. 3. When the scanning electoron microscopies were performed, it was observed that the cell-surface of the organisms exposed to the surfactant in one fiftieth and the surfactant containing 23 v/v% ethanol became to sticky and fusible. The morphological chang was greater in the former than in the later. Those findings indicate that the bactericidal activity of the surfactant against M. tuberculosis is enhanced by cooparation with ethanol.

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